ABSTRACT We analyzed the effect of obesity on daily physical activity (DPA), light-intensity physical activity (LPA), moderate-intensity physical activity (MPA) and vigorous- intensity physical activity (VPA) in children and adolescents. Overall, 462 students from a public school (53.6% boys; aged 7-12 years) were involved in this one-year longitudinal study. Physical activities and sedentary behaviors (SB) were reported in the questionnaire Food Intake and Physical Activity of Students (Web-CAAFE). Obesity was evaluated through Body Mass Index (BMI z score ≥ 2). Obesity was observed in 16.5% of participants, but it did not influence LPA, MPA or DPA. However, VPA were 20% less frequent among obese students (β2=0.80; CI 95%: 0.66-0.98). Obese girls showed less frequency of VPA than non-obese boys (Mean Difference = -0.97; CI 95%: -1.36 to -0.57). Non-obese girls showed less VPA than non-obese boys (Mean Difference = -1.18; CI 95%: -1.40 to -0.95) and obese ones (Mean Difference = -0.57; CI 95%: -0.90 to -0.24). Obese boys showed fewer VPA, if compared to non-obese ones (Mean Difference = -0.61; CI 95%: -0.96 to -0.26). The analysis of the obesity-sex-age interaction showed a greater reduction in VPA among obese girls aged 10 years or more when compared to obese girls aged 7-9 years, and among boys obese and non-obese regardless of age. Amounts of VPA and SB increased simultaneously among non-obese boys and non-obese children aged 7-9 years. Obesity reduced VPA in the sample during the follow up of one year, independently of age and SB.