Introduction: Angiogenesis is a dynamic process, essential for embryogenesis, morphogenesis, tumorigenesis and other biological processes, being the most important factor for growth and proliferation of tumors. Aim: Angiogenic growth factors vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) ensure vascularisation of the growing tumor tissue. The aim of our study was to analyze the relationship between the expression of VEGF and bFGF in pituitary adenomas cells and the corresponding serum levels in individual patients. The study also evaluated the potential of serum levels of the two growth factors as diagnostic markers. Materials and methods: VEGF and bFGF expression were determined by immunohistochemistry in 30 tumor samples, respectively. Serum VEGF and bFGF levels were determined in 20 pituitary adenoma patients and 10 healthy subjects, using a quantitative sandwich enzyme immunoassay technique. Results: Median values of VEGF serum levels in pituitary adenoma patients was 199.77 pg/ml, versus 95.45 pg/ml (healthy subjects). There is also a difference between median values of VEGF in invasive adenomas 213.1 pg/ml and non-invasive adenomas 159.77 pg/ml. Median values of bFGF serum levels in pituitary adenoma patients was 6.25 pg/ml, versus 4.64 pg/ml (healthy subjects). The mean of bFGF serum level was 6.95 pg/ml in invasive adenomas compared with 4.95 pg/ml in non-invasive adenomas. By immunohistochemistry VEGF and bFGF expression is more prominent in certain adenoma subtypes (functioning); stronger in invasive pituitary adenomas. Conclusions: A direct correlation was observed between VEGF and bFGF expression in pituitary adenomas cells, serum level of two growth factors and tumor behavior.