In this study, an uncertainty analysis for the infrared radiation characteristics of rocket exhaust plumes at representative trajectory points is performed. Owing to the instability of the rocket motor’s working characteristics, numerical rocket plume infrared radiation predictions possess significant uncertainties. In this study, four epistemic uncertain variables (freestream velocity, nozzle exit pressure, temperature, and velocity) are considered for uncertainty and sensitivity analyses. Based on the infrared signature analysis tool, the response surface of statistical samples is established through the point collocation nonintrusive polynomial chaos expansion method. Polynomial chaos expansion coefficients are solved using the quadrature method to calculate the statistical characteristics and uncertainty of random input variables. The tensor-product quadrature sparse grid method is utilized to reduce the number of samples for multiple input variables. Based on these models, the uncertainty quantification of infrared radiation for Atlas-IIA rocket plumes is analyzed, including the flows, radiation images, spectra, and radiance. The results show that the uncertainty mainly results from afterburning at low altitude, and the nozzle exit velocity has a significant influence on the radiation intensity of the plume. With an increase in altitude, the uncertainty of infrared radiation owing to the afterburning effect decreases, and the influence of the freestream velocity increases. In addition, the proportion of radiation intensity in the 4.3-μm band is higher than that in the 2.7-μm band, and the corresponding uncertainty band is gradually widened. The nozzle exit temperature is the dominant factor that affects the radiation characteristics of the plume at high altitudes. These results of uncertainty and sensitivity analyses are helpful for improving numerical models of the plume infrared signature.