Non-crosslinked Acellular Dermal Matrix Research Articles

Sacrocolpopexy: A Modification of the Standard Laparoscopic Procedure to Adopt It to the Properties of a Biologic Matrix Patch

Abstract Objective: By 80 years of age, 11.1% of women are expected to undergo pelvic organ prolapse (POP) surgery and one-third of women are expected to undergo reoperation. The “gold standard” procedure is sacrocolpopexy, which uses a graft to support the vaginal apex to the ligaments of the sacrum. The conventional graft (polypropylene mesh) has been associated with dyspareunia, persistent pelvic pain, and increased mesh erosion. In an effort to combat these complications, a noncrosslinked acellular dermal matrix (ADM; STRATTICE™ Reconstructive Matrix, LifeCell, an ACELITY Company, Bridgewater, NJ) was utilized for this study. Materials and Methods: Sacrocolpopexy, with ADM, was used for 9 patients between January 2011 and December 2013. The patients completed Pelvic Floor Distress Inventory-20 (PFDI-20) and pelvic floor impact questionnaires and reported on postsurgical outcome satisfaction. Patient satisfaction with clinical outcomes was assessed. Biopsies of the sacrocolpopexy were performed and tis...

Clinical research progress on tissue defect repairing with acellular dermal matrices

Acellular dermal matrix(ADM) is a special biological material that contains only collagen, elastin and other extracellular matrix. ADMs are made by special methods of separating skin epidermis and dermis, and removing Langerhans cells, vascular endothelial cells, fibroblasts cells and major histocompatibility complex (MHC) within the dermis. ADM causes no graft rejection, and provides a good scaffold for tissue reconstruction, angiogenesis and implantation of host cells. The preparation process of ADM includes the separation of epidermis and dermis, decellularization, perforation and freeze-drying. According to the classification method, ADMs can be classified into allogeneic and xenogeneic ADM, patch and injection type ADM, as well as cross-linked and non-crosslinked ADM, which can meet various clinical requirements. ADM is widely used in surgical field, such as treatment of deep burn wounds, breast reconstruction after mastectomy, oral mucosa repair, abdominal wall hernia repair, and treatment of vocal cord paralysis. Due to the characteristics ADM, its application prospect is considerable. The research progress of ADM in recent years was summarized. Key words: Acellular dermal matrix; Preparation; Clinical application

Levels of IL-4 and IFN-γ in serum of mice after the sheep skin acellular dermal matrix transplant

To evaluate the immunogenicity of self-made sheep skin acellular dermal matrix (ADM). Both cross-linked and non-crosslinked ADMs were made after the sheep skin ADM was ready. A total of 160 healthy mice were then randomly divided into 4 groups. Group A, cross-linked ADM was implanted under skin; Group B, non-crosslinked ADM was implanted under skin; Group C, allosome skin was embedded under skin; Control group, without any implantation. Any visible changes in implantation region and any apparent rejection was recorded. Any symptoms of inflammatory reaction in implantation region were also observed by microscope. The levels of IL-4 and IFN-γ in serum were measured on the 3rd day, 1 week, 2 weeks and 3 weeks after the operation, respectively. Visual study: wound healing in implantation regions were obvious, the immunologic rejection was not significant, and the sheep ADM was absorbed gradually by ambient tissues. Microscope results: the connection between the sheep ADM and surrounding tissues was very tight with infiltration of a few inflammatory cells into the depth of sheep ADM. The structure of sheep ADM was gradually destroyed by inflammatory cells. The levels of IL-4 and IFN-γ were not significant difference between the group of A, B or C and the control group on the 3rd day after the operation. The inflammatory reaction in implantation region of the control group degraded after 3 days, concomitant with a decrease in the levels of IL-4 and IFN-γ. But the levels of IL-4 and IFN-γ in the Group A, B or C remained at high level, suggesting the elevation of IL-4 and IFN-γ levels were related to the immunologic reaction by sheep ADM. The values of IL-4/IFN-γ were significant difference between the group A, B or C and the control group at the end of the 1st week, 2nd week, and 3rd week. However, there were not significantly different among the Group A, B and C. Sheep skin ADMs do not cause apparent rejection from mice after the transplantation because of the low immunogenicity. The cross-linked and non-crosslinked ADMs do not obviously affect the levels of IL-4 and IFN-γ.

Successful Integration and Revascularization of a Porcine Non-crosslinked Acellular Dermal Matrix in High Risk Ventral Hernia Repairs

Successful Integration and Revascularization of a Porcine Non-crosslinked Acellular Dermal Matrix in High Risk Ventral Hernia Repairs