Non-conventional Techniques Research Articles

Bioactive Compounds of Sanrego (Lunasia Amara Blanco.) Extracted Using Different Methods: A Review

The use of plants as medicine has been trusted by the community since time immemorial. Sanrego (Lunasia amara Blanco.) is one plant that has been believed to be an aphrodisiac. Sanrego is a wild plant found in the forests of Sulawesi, Java, Borneo, Maluku, Philippines, Papua New Guinea and Australia, and belongs to the Rutaceae Family. Sanrego has several properties such as treating stomach pain, anti-inflammatory, anti-cancer, snake bites and increasing sexual aggressiveness. This is because sanrego has bioactive ingredients as pharmacological. Sanrego extract contains secondary metabolites or bioactive compounds that include flavanoids, tannins, alakloids, steroids and saponins. Given the large variety of bioactive compounds in a sanrego plant, it is necessary to pay attention to extraction techniques in filtering these compounds. Some environmentally friendly extraction methods that can be used include maceration extraction methods, soxhlet, reflux, ultasonic, microwave, and enzyme assistance. This review discusses and describes the various extraction techniques used to obtain plant bioactive compounds. These extraction techniques include conventional extraction techniques and non-conventional extraction techniques. This review analyzes the advantages and disadvantages of this extraction technique. This review covers the application of extraction methods to sanrego plants.

Hybrid Warfare: Strategies and Counterstrategies in the India-Pakistan Rivalry

Hybrid Warfare represents a sophisticated amalgamation of diverse warfare strategies, seamlessly blending both conventional and non-conventional techniques. This nuanced approach encompasses an array of tactics, incorporating elements from fake news to cybercrimes, civil unrest to economic warfare, and diplomacy to separatist movements. Also recognized as asymmetrical or new-generation warfare, its primary objective is not confrontation but to meticulously identify and exploit the fault lines, vulnerabilities, and trigger points within the targeted nation. Pakistan has been subject to hybrid security threats from its historic rival India since the day of independence. The paper sheds light on Indian hybrid warfare against Pakistan which broadly encompasses political and diplomatic support to the separatist movements, targeted campaigns on social media, and hindering the timely completion of CPEC and other developmental projects. Given the intensity of hybrid security threats and their fall-flanged impact on the peace, development, and security of the state, the article has attempted to provide comprehensive policy options for Pakistan to effectively counter the hybrid threats.

Sensitivity of non-conventional electro-diagnostic techniques to detect neuropathy in patients with hepatitis “C” viral infection

BackgroundHepatitis “C” virus (HCV) infection has become a growing global health concern. Chronic HCV infection is usually accompanied by extra-hepatic manifestations. Peripheral neuropathies are the most common neurological complications. Electrophysiological tests play an important role in diagnosing polyneuropathy and identifying its pathophysiology. This study aimed at highlighting the sensitivity of non-conventional, quantitative, electrophysiological techniques in the detection of neuropathy in patients having HCV infection. Forty-five HCV patients were recruited for this study. Conventional motor and sensory conduction studies (NCS) were initially performed followed by interference pattern analysis (IPA). Two quantitative nerve conduction scores were also studied: motor amplitude score (MAS) and sensory amplitude score (SAS).ResultsIn the two patients’ subgroups (having numbness versus having no numbness), MAS showed a significant difference, while the SAS showed a non-significant difference. In the two subgroups (with normal NCS versus those with abnormal/suspected NCS), the MAS and the SAS mean values were significantly different. Also, there was a positive significant correlation found between the IPA mean amplitude and the SAS parameter. Finally, we presented the results of the diagnostic performance of the MAS and the SAS in detecting abnormal NCS.ConclusionsMAS and/or SAS scores could be used as good screening tools for the detection of neuropathy. We here recommend expanding the non-conventional NCSs to other etiologies of polyneuropathy.

Investigation on the impact of sub-zero treated electrodes in EDM

ABSTRACT Owing to the challenges faced during conventional machining of Titanium (Ti) alloys due to their low thermal conductivity and high chemical reaction, non-conventional machining techniques are used. Electrical Discharge Machining (EDM) is a promising solution that can precisely machine hard materials into intricate geometries but faces lower material removal rate, higher tool wear rate, and poor surface quality. In this research work, the effect of sub-zero treatments on EDM electrodes was investigated, and experimental investigations were conducted to identify the impact of sub-zero treated electrodes during EDM of Ti-6Al-4V under different discharge energy regimes. Peak Current, Pulse-On-Time and Gap Voltage were varied in 3 levels to evaluate the effect on MRR and TWR. A comprehensive study on EDMed surfaces has been assessed using the morphological and metallurgical observations from SEM micrographs, EDAX and XRD patterns. Sub-zero treated electrodes outperformed the Untreated (UT) electrodes regarding erosion rates and surface quality.

Design And Development Of A Spiral Tube Water Wheel Pumping System: A

This paper provides an overview of the ancient evolution,design and development of different types of spiral tube water wheel pumping system (STWWPS). It has always been a challenge to lift water and carry it to some other locations for executing meaningful work, making use of the alternative sources of power. Several kinds of non-conventional energy sources and techniques have been tried. Each one is having its own merits and demerits. Making use of the kinetic energy of flowing water can be achieved through different kinds of water wheel.Spiral tube powered by kinetic energy of flowing water has been utilized for lifting and carrying water for irrigation and other purposes, contributing to the replacement of conventional diesel and electric power sources. These conventional sources are expensive (Diesel) and often unreliable in rural areas (Electricity).This review paper examines the design, development, and performance evaluation of various spiral tube pumping systems, including single, double, multilayer, pedal type, four scoop type, and multi-purpose models. It examines the load torque, power, efficiency, and stress analysis of shaft and bearing, and the impact of rotational speed, submerged ratio, and number of spiral pipes on the performance of the pumping system.The working principle allows these pumps to create a column of water within its coil that alternatively the air which is compressed as it moves towards the center of wheel. These pumpsnot only save electricity and diesel cost but the maintenance cost is also lowapart from contributing to the clean environment.

NADES-Assisted Extraction of Polyphenols from Coriander Seeds: A Systematic Optimization Study.

Coriandrum sativum L. seeds are widely recognized for their traditional use in medicine. Among the most investigated components, the terpenoid linalool and monounsaturated petroselinic acid have attracted interest for their nutritional value. Instead, minor attention was paid to the polyphenolic fraction, resulting still being incomplete today. This study aimed to develop a systematic approach in which green natural deep eutectic solvents (NADES) were combined with conventional (maceration, MAC) or non-conventional (ultrasound-assisted extraction, UAE) techniques in a one-step methodology to recover polyphenols from coriander seeds. The NADES system choline chloride-citric acid (ChCl:CA, 1:1) was firstly evaluated, coupled with MAC or UAE, and then compared with ChCl-Urea (ChCl:Ur, 1:1) and ChCl-Glucose (ChCl:Glu, 1:1) under optimal conditions (20 min extraction time). The system ChCl:Ur UAE significantly improved the extraction of chlorogenic acid and its isomer (453.90 ± 4.77 and 537.42 ± 1.27 µg/g, respectively), while the system ChCl:Glu UAE improved the extraction of protocatechuic, caffeic and p-coumaric acids (131.13 ± 6.16, 269.03 ± 4.15 and 57.36 ± 0.06 µg/g, respectively). The highest levels of rutin were obtained with ChCl:CA-based NADES when the MAC technique was applied (820.31 ± 28.59 µg/g). These findings indicate that the NADES composition could be appropriately modulated to tailor extraction towards higher levels of a desirable bioactive for further applications.

Parametric investigation and optimization in laser based directed energy deposition of tungsten carbide-cobalt

Cemented carbide (WC-Co), the widely used tool-die material, is difficult to be machined by conventional and nonconventional techniques. This inspired exploring additive manufacturing (AM) of this material. However, porosity, brittleness due to cobalt depletion, etc. have been reported in the literature with rare success. For the AM of WC-Co, the current work focuses on directed energy deposition, which can be implemented with existing laser cutting-welding workstations, with modifications. To ensure the retention of cobalt even after inevitable vaporization of some of its initial content during deposition, 20 wt. % of Co was mixed with WC powder by low-energy ball milling. Laser power, scan speed, and powder flow rate were varied following a full-factorial design of experiments. The analysis of variance revealed that the experimental model and most of the parameters were significant. Only the laser power came out to be insignificant for the contact angle. The track height and width increased with the laser power and reduced with the scan speed. The contact angle increased with the scan speed and reduced with the powder flow rate. Cross sections of the deposited track showed no pores or cracks. Multiobjective optimization with gray relational analysis was conducted to get the parameter combination giving high values of the contact angle, track height, and width simultaneously. The optimum parameter combination, thus, obtained is 700 W laser power, 5 mm/s scan speed, and 5 g/min powder flow rate. This yielded 305 ± 40 μm track height, 2132 ± 33 μm width, and 152° ± 2° contact angle.

Development of Non-Conventional Method of Lissajous pattern for gear fault diagnosis using Machine Learning Technique

Gears are major elements in machine parts and automotive systems, to transmitting torque and energy between components. As gears are under severe loads and stresses, they can create different types of faults and defects over period, impacting their own performance and reliability. Fault detection is an essential part of gear maintenance because that allows potential issues to be detected before they induce failures or expensive breakdowns. Technicians can identify early indications of wear, imbalance, damage, or even other concerns that might compromise their performance or lead to premature failure by monitoring the condition of gears. The industry has conventionally used vibration signals, thermography, oil analysis, and visually inspecting to diagnose faults. These techniques also have significant limitations which can decrease their accuracy in detecting faults and the cost is also very high. To overcome these limitations, new methods such as Lissajous pattern developed for fault detection in gears. These techniques have proved impressive outcomes in fault detection which have been previously undetectable using Conventional methods. This study evaluates the accuracy, performance, and cost-effectiveness of conventional and non-conventional techniques to determine the best method for fault diagnosis. Once compared to the conventional method, the Lissajous method obtained better accuracy than conventional method.

State and role of water confined in cement and composites modified with metakaolin and additives

Cement as a porous material contains pores and cracks on the whole nanoscale forming an interconnected pore structure. Its interaction with water during the rehydration of cement plays a key role in every application, such as in nuclear waste treatment, where cement binders are commonly used for conditioning liquid radioactive waste. In the prediction of the long-term disposal the knowledge of water mobility and distribution in the formed porous cement matrix is essential.In this study we systematically investigate the effects of metakaolin and several components of the liquid phase (complexing agents, model ions, boric acid, etc.) on the hydrated solid structure by various nuclear magnetic resonance (NMR) methods. The novelty lies in the complex use of such non-conventional techniques that separately provide only partial information of the cement structure; however, the gained results complete each other to offer a new approach in the structural study under several conditions. Water mobility is deduced from NMR T2 relaxation distributions and D2O-H2O exchange diffusion measurements, and the conclusion on structural properties is supported by the macropore structure revealed from NMR cryoporometry and SEM (scanning electron microscopy) images, as well as by following the formation of pores and water domains in the curing process with NMR relaxometry. The results confirm that metakaolin was built into the CSH (calcium silicate hydrate) gel region without altering its mesoporous structure, however, it significantly decreased the capillary pore size and the diffusion coefficient of water. Complexing agent concentration between 2 and 20 g/l and the presence of model ions did not cause significant structural changes, while the rehydration decreased the size of capillary pores due to swelling.

Extraction of dietary fiber from kinnow (Citrus reticulata) peels using sequential ultrasonic and enzymatic treatments and its application in development of cookies

The paradigm shifts from discarding horticulture byproducts to exploiting them for their bioactive potential has encouraged research on non-conventional extraction techniques such as extraction using microwave, ultrasound, high-pressure, etc. Kinnow peels, a primary residue of kinnow processing industry, are profuse with polysaccharides, such as dietary fiber which can be isolated and used as a functional ingredient in fortified/enriched foods. In the present study, ultrasound-assisted extraction (UAE) and ultrasound-assisted-enzymatic extraction (UAEE) have been optimized to maximize dietary fiber yield from kinnow peel waste. Total dietary fiber (TDF) of 52.042 ± 0.862% was observed using UAE at 38% amplitude, liquid to solid ratio (LSR) of 40 mL/g, at 44 °C, after sonication time of 13 min, which increased to 60.974 ± 0.827% upon sequential enzymatic treatment. The WHC, OHC, and GAC of extracted dietary fiber ranged between 8.17 and 9.24 ± 0.11 g/g, 2.96–4.68 ± 0.09 g/g, and 1.56–2.28 ± 0.04 mmol/g. The physicochemical characterization of dietary fiber using analytical instruments and its functional properties such as oil/water holding, and glucose adsorption capacities proved that kinnow peels dietary fiber can be used to develop fiber-enriched products. The extracted fiber has been used to develop cookies which showed good nutritional, sensory, and textural properties with an overall acceptability of 7.15–7.84. This research will be helpful in scale-up studies on the valorization of different citrus peels through an extraction approach.

Innovative methods for extraction of essential oils from medicinal plants

Essential oils are concentrated liquids of intricate combinations of volatile substances, extracted from various plant parts. Several bioactive substances with antibacterial and antioxidant activities are abundant in essential oils. Some essential oils have also been employed in medicine. Due to the risk associated with employing synthetic preservatives, the use of essential oils as natural additives for extending the shelf life of food products has also drawn considerable attention. They are used in the pharmaceutical, cosmetic, and food industries for their functional properties. There are various methods for extraction, but both the quality and the percentage yield of essential oil never remain the same. So, innovative and non-conventional techniques of essential oils extraction from medicinal plants were evoled to get quantitative and qualitative yield. In the present article, we searched and reviewed innovative techniques used for the extraction of essential oils from medicinal and aromatic plants through electronic searches of PubMed, Medline, Wiley, Scopus, and Google Scholar. For the extraction of essential oils, several innovative/non-conventional techniques have been reported in literature. Extraction of essential oil by using innovative techniques retards the risk of losing the essential components of plants, maintains the quality, reduces chemical risk, extraction time, acts eco-friendly, and increases the percentage yield of the essential oils. This paper presents the success story of innovative extraction methods of essential oils in accordance with sustainable development and environmental protection.

Investigation of the composition, structure and magnetic properties of the Nd2Fe14B ceramics dependence on the initial powder characteristics and spark plasma sintering modes

High phase and structural sensitivity of magnetic properties strongly impacts the fabrication quality of the permanent magnets, therefore revealing effects of synthesis conditions on physico-chemical characteristics of magnetic materials is of paramount importance for optimization of magnet production especially in case when non-conventional techniques are involved. Here we report for the influence of pulse current frequency on structure, composition, and magnetic properties of an industrially-important Nd2Fe14B magnetic ceramics on the initial powder characteristics and the modes of its spark plasma sintering (SPS) in a vacuum environment. The influence of initial powder mechanical grinding as well as the number of generated electric current pulses (in the range from 3.3 to 326.7 ms) in SPS conditions on the structure (defects and pores formation, grain size), phase and element composition (formation of secondary nonmagnetic phases and oxides, impurities) of Nd2Fe14B alloy was studied by SEM, TEM, EDS and XRD methods. The regularity of changes in the magnetization saturation value (σ) and coercive force (Нс) depending on the morphology, structure and composition of the initial Nd2Fe14B powder and the alloy obtained by mechanical milling and subsequent sintering at different modes of pulse current has been established.

A comprehensive review of conventional and non-conventional solvent extraction techniques

A comprehensive review of conventional and non-conventional solvent extraction techniques

Non-conventional machining of nickel based superalloys: A review

Nickel, the 24th most abundant element, is found in 0.016 % of the earth's crust. Addition of Nickel is used as an alloying element in hardenable steels, stainless steels, high-temperature alloys,corrosion-resistant and“nickel-silver,” and aluminium-nickel alloys. Nickel improves the toughness and ductility of cast iron. Nickel-base super alloys (Inconel) are widely regarded as one of the most difficult materials to machine because of their extreme hardness, high strength at high temperatures, tendencyto react with tool materials, and low thermal diffusivity. Because of various issues that arise during the machining process, nickel-based superalloys are regarded as difficult to machine materials; heat will advance into the tool material, causing tool wear tear and premature tool failure. To overcome these challenges, a non-conventional machine process is beneficial for super alloy machining. The present study is review of various nickel-based super alloys and their types, introduction to the non-conventional machining techniques applied to superalloys over the past few decades. Machining with non-conventional methods concludes with some key findings and gaps regarding nickel-based superalloys.

Green Solvents: Emerging Alternatives for Carotenoid Extraction from Fruit and Vegetable By-Products

Carotenoids have important implications for human health and the food industry due to their antioxidant and functional properties. Their extraction is a crucial step for being able to concentrate them and potentially include them in food products. Traditionally, the extraction of carotenoids is performed using organic solvents that have toxicological effects. Developing greener solvents and techniques for extracting high-value compounds is one of the principles of green chemistry and a challenge for the food industry. This review will analyze the use of green solvents, namely, vegetable oils, supercritical fluids, deep eutectic solvents, ionic liquids, and limonene, combined with nonconventional techniques (ultrasound-assisted extraction and microwave), for carotenoid extraction from fruit and vegetable by-products as upcoming alternatives to organic solvents. Recent developments in the isolation of carotenoids from green solvents and their inclusion in food products will also be discussed. The use of green solvents offers significant advantages in extracting carotenoids, both by decreasing the downstream process of solvent elimination, and the fact that the carotenoids can be included directly in food products without posing a risk to human health.

Step-by-Step Modeling and Experimental Study on the Sol–Gel Porous Structure of Percolation Nanoclusters

Non-conventional crystallization techniques have been developed in recent years. Non-conventional crystallization techniques use primary structural elements (for example, clusters) rather than atoms and molecules. Modern nanomaterial science is going through great changes as an entirely new approach of non-conventional growth mechanisms is emerging due to cluster coupling, catalyzing interest in cluster physics. The formation of fractal and percolation clusters has increased. We carried out step-by-step modeling and an experimental study of the formation of fractal and percolation clusters based on tin dioxide and silicon dioxide and formed by sol–gel technology. In this paper, the growth of fractal aggregates (clusters) from sol particles SnO2 and SiO2 based on the modified models of diffusion-limited and cluster–cluster aggregation is discussed. A percolation model using simulated fractal clusters of SnO2 and SiO2 particles is proposed. Experimental data on the sol–gel percolation structure of porous nanocomposites are presented. The modeling of SnO2 and SiO2 particles, which also consist of clusters (the next step in the hierarchy), is shown. We propose a generalized hierarchical three-dimensional percolation cluster model that allows calculating the surface area, knowing the experimental sizes of macropores and taking into account the micro- and mesopores (sizes less than a few nanometers).

Impact of the Extraction Method on the Chemical Composition and Antioxidant Potency of Rosmarinus officinalis L. Extracts.

Rosmarinus officinalis L. is a dietary source that produces polyphenols as secondary metabolites. These natural compounds with potent antioxidant abilities are increasingly recommended as a supplement to inhibit oxidative stress. In the current work, we evaluated the impact of the extraction method on the chemical composition of R. officinalis extract, especially on the content of carnosic (CA) and rosmarinic (RA) acids using UPLC-MS-DAD as well as on their antioxidant potency. Four extracts of Tunisian rosemary were obtained from non-conventional extraction techniques:ultrasound-assisted extraction (UAE),supercritical extraction (SFE) and UAE and SFE combined ((UAE-SFE(I), UAE-SFE(II)). The UAE exhibited the best total phenolic compounds (i.e., 85.27 mg GAEg-1), the highest content of CAand RA and the strongest antioxidant abilities (i.e., IC50 = 0.13 mg/mL and EC50 = 0.93 mg/mL for DPPH scavenging test and iron reducing power ability assay). The evaluation of antioxidant activity of UAE inhuman skin fibroblast (HS-68) cell line was carried out after the induction of oxidative stress. The results determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed a strong protective effect against H2O2oxidative stress induction in cells pretreated with UAE. The obtained results allow us to give new insight about the effect of the extraction method on the chemical composition and biological activities of the extract and the importance of the choice of the most appropriate processing technique to prepare rosemary extract with a high antioxidant potency and protective effect against oxidative stress.

Parametric optimisation of plasma polishing process using response surface methodology

ABSTRACT The plasma polishing process is one of the non-conventional techniques used to remove material at the atomic level from the substrate. During the polishing of the fused silica substrate, the process parameters, namely radio-frequency (RF) power, pressure ratio (SF6/O2), and total pressure of the plasma chamber, are investigated and optimised for material removal rate (MRR) and % change in surface roughness (% ΔRa ) using response surface methodology. The optimum values obtained for MRR and % ΔRa are 0.012 mm3/min and 3.59, at RF power of 60 W, pressure ratio of 3, and total pressure of 14.3 mbar. The experimental results reveal that surface roughness slightly increases from 0.344 to 0.356 μm after plasma processing at optimised process conditions. Moreover, the plasma-processed fused silica substrate is characterised using field emission scanning electron microscopy and energy dispersive X-ray spectroscopy, which depict the presence of silicon, oxygen, and fluorine on the processed substrate.

Co-extraction of soy protein and polysaccharide with lipid-lowering activity: Characterization of functional property, nutritional property and colonic fermentation property through a metabolomics approach

Defatted soy meal is a candidate for preparing lipid-lowering active ingredient. The study aimed to optimally co-extract soy protein and polysaccharide with lipid-lowering activity and improved functional and nutritional properties using non-conventional extraction techniques, i.e. thermal-, high pressure homogenization- and enzyme-assisted extraction. Non-conventional extraction enhanced the yields of soy protein and soluble saccharides and inactivated anti-nutrients. Soy extract (SE) prepared by pectinase-assisted extraction possessed not only strong in vitro lipid-lowering activity (i.e. cholesterol micellar inhibition activity, bile salts binding capacity, pancreatic lipase inhibition activity and cholesterol esterase inhibition activity), but also excellent functional (i.e. instant solubility of protein, low turbidity and acceptable color) and nutritional properties (i.e. none agglutinin activity). Resistant-digested fragment of 7S/11S played key role in lipid-lowering activity of SE. Moreover, SE beneficially altered colonic fermentation property of soy protein through promoting the release of bioactive peptides and organic acids, accelerating tryptophan and linoleic acid metabolism to produce indole acrylic acid, indole lactic acid and linoleic acid, and inhibiting putrefactive compounds (i.e. piperidine and tyramine). Oligosaccharide and polysaccharide in SE improved functional and nutritional properties of soy protein without compromising its lipid-lowering activity.

Optimization of Ultrasound- and Microwave-Assisted Extraction for the Determination of Phenolic Compounds in Peach Byproducts Using Experimental Design and Liquid Chromatography-Tandem Mass Spectrometry.

The conversion of plant byproducts, which are phenolic-rich substrates, to valuable co-products by implementing non-conventional extraction techniques is the need of the hour. In the current study, ultrasound- (UAE) and microwave-assisted extraction (MAE) were applied for the recovery of polyphenols from peach byproducts. Two-level screening and Box-Behnken design were adopted to optimize extraction efficiency in terms of total phenolic content (TPC). Methanol:water 4:1% v/v was the extraction solvent. The optimal conditions of UAE were 15 min, 8 s ON-5 s OFF, and 35 mL g-1, while MAE was maximized at 20 min, 58 °C, and 16 mL g-1. Regarding the extracts' TPC and antioxidant activity, MAE emerged as the method of choice, whilst their antiradical activity was similar in both techniques. Furthermore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine chlorogenic acid and naringenin in byproducts' extracts. 4-Chloro-4'-hydroxybenzophenone is proposed as a new internal standard in LC-MS/MS analysis in foods and byproducts. Chlorogenic acid was extracted in higher yields when UAE was used, while MAE favored the extraction of the flavonoid compound, naringenin. To conclude, non-conventional extraction could be considered as an efficient and fast alternative for the recovery of bioactive compounds from plant matrices.