Pastoralism Research Articles

Time for a change? Investigating shifts in agricultural economies and food in southern-central Norway (11th -16th c. AD)

Cattle and, to a lesser extent, sheep/goat and pigs, were crucial components of Norway’s socio-cultural and economic dynamics in the Middle Ages. Zooarchaeological analyses of faunal samples from different urban sites reveal that changes in cattle husbandry practices occurred in Norway over the medieval period, and especially in its early phases. This is clearly demonstrated in the case of medieval Oslo, where an in-depth zooarchaeological study provides the first comprehensive review of cattle husbandry strategies. In the first centuries of the medieval period (11th -mid 12th c. AD), cattle were mainly raised for milk and meat production, while fewer young and more numerous older individuals, most of which probably oxen, were present from mid 12th -13th c. AD onwards. This hypothesis is supported by biometrical analyses of cattle postcranial bones, indicating changes in the sexual composition of cattle herds over time. Therefore, from ca. mid 12th -13th c. AD onward, cattle seem to have been more used for ploughing and, only once old, to have been culled for their meat. In turn, this evidence suggests that more arable lands had to be ploughed, especially in eastern and central Norway. Here, demographic growth and settlement expansion would have led to an increase in food demand; at the same time, more favourable climatic conditions might have contributed to an increase in the availability of arable fields. This article suggests that medieval Norwegian farming was not static as historically thought, but rather connected to wider-scale agricultural innovations characterising other parts of medieval Europe.

Полиморфизм гена GDF5 крупного рогатого скота калмыцкой породы и его влияние на экстерьерные показатели

Purpose. Study of the influence of polymorphism of GDF5 gene of Kalmyk cattle on exterior characteristics. Materials and Methods. The polymorphism of the GDF5 gene was examined in 60 individuals of Kalmyk cattle breed belonging to the APC “Plodovitoe” of the Maloderbetovsky district of the Republic of Kalmykia. DNA samples were isolated from whole blood collected from the jugular vein using an automated extraction method. Real-time polymerase chain reaction was performed to determine the T586C polymorphism of the GDF5 gene. Results. Among the studied herd of cattle, individuals with the TT genotype predominated - they accounted for 68.3% of the total. Animals with TC and CC genotypes accounted for 21.7 and 10.0%, respectively. The frequency of occurrence of the T allele of GDF5 gene was 0.792, which exceeded the frequency of the C allele (0.208) by 58.2%. The observed frequency of the TT and CC genotypes exceeded the expected ones by 5.6 and 5.7%, respectively. At the same time, the frequency of the TC genotype was lower than expected by 11.2%. Individuals with the TT genotype of the GDF5 gene were superior to their peers with the TC and CC genotypes in all measured parameters. Conclusion. TT genotype of GDF5 gene in the studied livestock of Kalmyk cattle breed, owned by the APC “Plodovitoye”, occurs with the highest frequency (0.683). A positive effect of the TT genotype of GDF5 gene on linear physique measurements of Kalmyk cattle was revealed.

Seroprevalence of bovine leukemia virus and association with bovine infectious abortion in Creole breeds from tropical grazing herds in the Colombian Caribbean.

In the Caribbean region of Colombia, the concomitance of endemic infectious agents is a common problem, and coinfections are possible, increasing the complexity of cattle herds' sanitary, reproductive, and productive problems. This study aimed to estimate the seroprevalence of bovine leukemia virus and its association with bovine infectious abortion in grazing Creole breeds from tropical herds in the Colombian Caribbean. For the determination of bovine leukemia virus (BLV), bovine viral diarrhea virus (BVDV), bovine herpes virus-1 (BoHV-1), and Neospora Caninum (NC), the enzyme-linked immunosorbent assay technique was used. Matrix analysis was performed to represent multiple seroprevalence in the same cow. To explore the association between the seroprevalence of BLV and bovine infectious abortion agents, a multivariate logistic regression model was used. The seroprevalence was as follows: BLV 30.78%, BVDV 33.01%, BoHV-1 12.85%, and NC 8.96%. In the multivariate logistic regression model, seroprevalence of BVDV (OR 10.8; 95% CI: 7.5-15.6) and seroprevalence of BoHV-1 (OR 1.8; 95% CI: 1.1-3.0) were associated with the seroprevalence of BLV. Animals infected with BLV are more susceptible to coinfections with BVDV and BoHV-1. Implementing healthy measures against these two immunosuppressive infections could enhance the hygiene of numerous cattle herds. This study was designed as a retrospective cross-sectional study, which limits the ability to confirm that BLV is the primary infection. Further studies to confirm the primary infection of BLV with an active viral coinfection are necessary and the factors associated with these phenomena.

Wildlife corridors in a Southern African conservation landscape: the political ecology of multispecies mobilities along the arteries of anthropogenic conservation

The decline of biodiversity is a key topic in public discussions around the globe. These debates have triggered massive efforts to increase protected areas and to safeguard the corridors connecting them. The wildlife corridors dealt with in this article are mainly thought to facilitate the mobility of elephants and some other large herbivores (for example, zebra and buffalo). Wildlife corridors are not only essential for species connectivity but also an integral part of the booming ecotourism in north-eastern Namibia’s conservation landscapes. Coexistence infrastructure is meant to contribute to economic development and local incomes. Conservancies — community-based conservation organisations in the Namibian context — gazette corridors and market wildlife abundance to ecotourists, potential investors in tourism and commercial hunters. The coexistence of humans and wildlife is challenging, though. Human-wildlife interactions frequently result in damage, and often conservationist environmental infrastructuring runs against the aims of farmers to expand their fields for commercial crop production and to gain pastures for growing cattle herds. It also runs against other governmentally endorsed infrastructuring that brings tarred roads, water pipelines and boreholes. This article analyses contested wildlife corridors as part of a larger conservationist project in the western parts of Namibia’s Zambezi Region.

Constraints Associated with the Adoption of Sustainable Cassava Weed Management Technology for Cassava Systems in Nigeria

The study investigated the constraints associated with the adoption of sustainable cassava weeds management technology for cassava systems (SCWMTCS) in Nigeria. Multistage was utilized in selecting 384 respondents for the study. Descriptive and inferential statistics were used to analyse the data collected. Results revealed that respondents have varied perceptions of the severity of constraints encountered in their process of adoption of SCWMTCS in the study area. Constraints comprising of frequent gluts in cassava market frustrates farmers, lack of processing equipment and poor value chain system for cassava products and frequent encroachments or destructive grazing of cassava farms by nomadic herders is very frustrating were rated high severity in the study area. Also, 17 constraints were rated to be moderately severe in the study area out of the 26 listed constraints encountered by cassava farmers.

Risk-Based Targeting of Animals for Ancillary Testing during a Bovine Tuberculosis Breakdown Is Associated with a Reduced Time to Test Failure: Indirect Evidence of Mycobacterium bovis Exposure?

Bovine tuberculosis (bTB) continues to have significant economic and veterinary health impacts on cattle herds where the disease remains endemic. The continual tailoring of policies to address such maintenance requires an in-depth analysis of national data, underpinning new control strategies. In Ireland, when outbreaks occur, ancillary testing of herd mates deemed to be at the highest risk of exposure to reactors is undertaken using the interferon gamma (GIF) test. This highest risk cohort was hypothesised to be of a higher future risk despite this ancillary testing. We used a dataset from Ireland to model bovine test failure to the comparative tuberculin skin test using a survival analysis (observations: 39,248). Our primary exposure of interest was whether an animal that tested negative had a GIF test after the disclosure of infection within a herd during a bTB breakdown. There was evidence that animals with a negative GIF test during a breakdown had an increased risk of failing a test relative to other animals from the same herds without this exposure. The time to failure was 48.8% (95%CI: 38.3-57.5%) shorter for the exposed group relative to the unexposed group during a two-year follow-up period (2019-2022; time ratio: 0.51; 95%CI: 0.43-0.62; p < 0.001). The results from this study suggest that animals who were GIF-tested, having been deemed to have a higher risk of exposure, subsequently had shorter time-to-test failure periods. The absolute numbers of failure are small (only 2.5% of animals go on to fail during 2-year follow-up). Importantly, however, a high proportion of these high-risk herds included in the dataset failed at least one test at the follow-up (21/54 herds), impacting breakdown duration or recurrence. Such risk-informed targeting of animals could be utilised in future control policies, though further research is warranted.

Faecal microbial diversity in a cattle herd infected by Mycobacterium avium subsp. paratuberculosis: a possible effect of production status

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, or paratuberculosis (PTB) in ruminants, besides having zoonotic potential. It possibly changes the gut microbiome, but no conclusive data are available yet. This study aimed at investigating the influence of MAP on the faecal microbiome of cattle naturally infected with PTB. In a follow up period of 10 months, PTB status was investigated in a herd of dairy cattle with history of clinical cases. Each animal was tested for MAP infection using serum and milk ELISA for MAP anti-bodies and IS900 real-time PCR and recombinase polymerase amplification assays for MAP DNA in the faeces and milk monthly for 4 successive months, then a last one after 6 months. The faecal samples were subjected to 16S rDNA metagenomic analysis using Oxford Nanopore Sequencing Technology. The microbial content was compared between animal groups based on MAP positivity rate and production status. All animals were MAP positive by one or more tests, but two animals were consistently negative for MAP DNA in the faeces. In all animals, the phyla firmicutes and bacteroidetes were highly enriched with a small contribution of proteobacteria, and increased abundance of the families Oscillospiraceae, Planococcaceae, and Streptococcacaceae was noted. Animals with high MAP positivity rate showed comparable faecal microbial content, although MAP faecal positivity had no significant effect (p > 0.05) on the microbiome. Generally, richness and evenness indices decreased with increasing positivity rate. A significantly different microbial content was found between dry cows and heifers (p < 0.05). Particularly, Oscillospiraceae and Rikenellaceae were enriched in heifers, while Planococcaceae and Streptococcaceae were overrepresented in dry cows. Furthermore, abundance of 72 genera was significantly different between these two groups (p < 0.05). Changes in faecal microbiome composition were notably associated with increasing MAP shedding in the faeces. The present findings suggest a combined influence of the production status and MAP on the cattle faecal microbiome. This possibly correlates with the fate of the infection, the concern in disease control, again remains for further investigations.

An epidemiological investigation into the reasons for high bovine tuberculosis incidence in cattle herds of the Burren, Ireland, prior to 2020

Herd-level bovine tuberculosis (bTB) incidence was examined in the Burren, an area in the west of Ireland where herd owners practice distinctive transhumance practices, with upland winter grazing. Prior to the initiation of our study in 2020, bTB incidence had for many years been unusually high in the Burren in comparison with the rest of the country, although the most recent figures have come down to being closer to the national average. Using data from the period prior to 2020, we mapped bTB infection in Burren herds alongside a range of indicators thought to have an association with it - herd size, herd density, herd type, cattle movement, and badger (Meles meles) population and control data, as well as rainfall and altitude. We also looked at how summary statistics for these variables differed when Burren herds with a history of bTB were compared to other Burren herds, as well as bTB positive and negative herds from outside the Burren. We found that for many indicators Burren herds would be expected to be low risk when compared to other herds in Ireland. An exception to this was for rainfall: hot spot areas for bTB in the Burren were found in areas of higher rainfall, on average herds in the Burren experienced more rainfall than those outside it, and bTB herds in the Burren experienced higher rainfall than non-bTB herds. Separately, for Burren herds only, a logistic regression model was developed to explain bTB breakdown occurrence using a matched case-control approach. Cases were herds which had experienced a new bTB breakdown between 2015 and 2019 (n = 260) and these were matched on herd type and herd size with the same number of herds not experiencing a breakdown during this period. This showed that, of a range of exogenous variables, rainfall was the most strongly associated with herd-level bTB incidence. These results suggest that high levels of exposure to inclement weather, and/or better environmental survival of Mycobacterium bovis in the environment, may contribute to high bTB rates in the Burren. However, as rainfall showed a highly aggregated distribution, this relationship may be due to an unmeasured factor correlated with it. Mapping and graphical output suggested that, although herd sizes in the Burren were on average lower than nationally, within the Burren they were higher in areas of higher prevalence, suggesting that mechanisms associated with herd size, such as increased contacts between and within herd, and with wildlife, may also play a role.

Spatial Distribution and Hierarchical Behaviour of Cattle Using a Virtual Fence System.

Interest in virtual fencing has increased due to its flexibility for agriculture and rewilding. However, systems like Nofence© require large financial investments, and the need for individual collars complicates large-scale use. If cattle herds maintain cohesive groups around leading individuals, fewer collars could be used, thereby enhancing cost efficiency. This study investigates the pattern in spatial distribution in a herd of 17 Angus cows on Fanø in Denmark with GPS locations, using a Nofence© system. The aim of this paper is to determine how individuals position themselves in a herd, spatially, and identify a pattern in ranks. The method used in this study examines the distances between an individual to the rest of the herdmates using nearest neighbour and descriptive statistics. Contrary to expectations, this study did not reveal a distinct pattern in herd distribution. While some tendencies in spatial distribution patterns were observed, only a low concordance could be found (W=0.15,p<0.001), indicating great variability in the cattle's ranks. A cumulative curve of the ranks estimated over the entire periods, however, allowed a rough estimation of the hierarchy and allowed identification of the highest-ranked cows, making the use of a cumulative curve a possible solution to finding the high-ranked cows. This research underscores the complexity of cattle social structures and highlights the need for extended observation periods and alternative methodologies to enhance the cost-effectiveness and scalability of virtual fencing in agricultural and rewilding contexts.

Insights on early response to acute heat shock of bovine mammary epithelial cells through a multimethod approach

Heat stress is a significant challenge in dairy cattle herds, affecting milk production and quality, and generating important changes at the cellular level. Most in vitro research on heat shock (HS) effects on dairy cow mammary cells was focused on medium-long-term effects. In recent years, Fourier transform-infrared (FT-IR) micro-spectroscopy has been increasingly used to study the effects of several external stresses on different cell lines, down to the level of single cellular components, such as DNA/RNA, lipids, and proteins. In this study, the possible changes at the biochemical and molecular level induced by acute (30 min-2 h) HS in bovine mammary epithelial (BME-UV1) cells were investigated. The cells were exposed to different temperatures, thermoneutral (TN, 37 °C) and HS (42 °C), and FT-IR spectra were acquired to analyse the effects of HS on biochemical characteristics of BME-UV1 cellular components (proteins, lipids, and DNA/RNA). Moreover, cell viability assay, reactive oxygen species production, and mRNA expression of heat shock proteins (HSPA1A, HSP90AA1, GRP78, GRP94) and antioxidant genes (SOD1, SOD2) by RT-qPCR were also analysed. The FT-IR results showed a change already at 30 min of HS exposure, in the content of long-chain fatty acids, which probably acted as a response to a modification of membrane fluidity in HS cells compared with TN cells. After 2 h of HS exposure, modification of DNA/RNA activity and accumulation of aggregated proteins was highlighted in HS cells. The gene expression analyses showed the overexpression of HSPA1A and HSP90AA1 starting from 30 min up to 2 h in HS cells compared with TN cells. At 2 h of HS exposure, also the overexpression of GRP94 was observed in HS cells. Acute HS did not affect cell viability, reactive oxygen species level, and SOD1 and SOD2 gene expression of BME-UV1 cells. According to the results obtained, cells initiate early defence mechanisms in case of acute HS and probably this efficient response capacity may be decisive for tolerance to heat stress of dairy cattle.

Impact of reproductive traits on productive life in Iranian Holstein dairy cows.

Enhancing reproductive performance is a key strategy to mitigate involuntary culling rates, thereby extending productive life (PL) and ultimately improving profitability in dairy cattle herds. A piecewise Weibull proportional hazards model was used to investigate the effect of several important reproductive traits on PL in Holstein dairy cows. Data comprised 200,747 lactation records from 82,505 cows sired by 1952 bulls across 36 dairy herds. PL was defined as the number of days from the first calving to the last milk record or censoring. The statistical model accounted for the time-dependent fixed effects of changes in herd size, year-season, milk production, fat and protein contents, and the time-independent fixed effect of age at first calving. Herd-year and sire effects were also included as random effects. Reproductive traits include calving traits such as calving ease (CE), calf size (CZ), and calf survival (CS), as well as female fertility traits such as number of inseminations per conception (NI), days from calving to first service (CFS), days from first service to conception (FSC), and days open (DO). All reproductive traits had a significant effect on PL (p < 0.001). Each reproductive trait was analysed separately. The relative risk (RR) of being culled increased as the severity of calving difficulties increased in both primiparous and multiparous cows. Cows that calved small or large calves showed a higher risk of being culled compared with those that calved medium size calves. The increased RR of culling was observed only for primiparous cows that gave birth to dead calves. In addition, cows that required more NI, a longer CFS, FSC, and DO had shorter longevity. These insights can deepen our comprehension of the factors affecting PL and provide information for refining management and breeding strategies, which could lead to increased profitability and sustainability in Iranian dairy farming.

Non-invasive sampling based screening of dairy cattle herds for Mycobacterium sps

Non-invasive sampling based screening of dairy cattle herds for Mycobacterium sps

Integrated Analysis of Transcriptome Profiles and lncRNA-miRNA-mRNA Competing Endogenous RNA Regulatory Network to Identify Biological Functional Effects of Genes and Pathways Associated with Johne's Disease in Dairy Cattle.

Paratuberculosis or Johne's disease (JD), a chronic granulomatous gastroenteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), causes huge economic losses and reduces animal welfare in dairy cattle herds worldwide. At present, molecular mechanisms and biological functions involved in immune responses to MAP infection of dairy cattle are not clearly understood. Our purpose was to integrate transcriptomic profiles and competing endogenous RNA (ceRNA) network analyses to identify key messenger RNAs (mRNAs) and regulatory RNAs involved in molecular regulation of peripheral blood mononuclear cells (PBMCs) for MAP infection in dairy cattle. In total, 28 lncRNAs, 42 miRNAs, and 370 mRNAs were identified by integrating gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. In this regard, we identified 21 hub genes (CCL20, CCL5, CD40, CSF2, CXCL8, EIF2AK2, FOS, IL10, IL17A, IL1A, IL1B, IRF1, MX2, NFKB1, NFKBIA, PTGS2, SOCS3, TLR4, TNF, TNFAIP3, and VCAM1) involved in MAP infection. Furthermore, eight candidate subnets with eight lncRNAs, 29 miRNAs, and 237 mRNAs were detected through clustering analyses, whereas GO enrichment analysis of identified RNAs revealed 510, 22, and 11 significantly enriched GO terms related to MAP infection in biological process, molecular function, and cellular component categories, respectively. The main metabolic-signaling pathways related to MAP infection that were enriched included the immune system process, defense response, response to cytokine, leukocyte migration, regulation of T cell activation, defense response to bacterium, NOD-like receptor, B cell receptor, TNF, NF-kappa B, IL-17, and T cell receptor signaling pathways. Contributions of transcriptome profiles from MAP-positive and MAP-negative sample groups plus a ceRNA regulatory network underlying phenotypic differences in the intensity of pathogenicity of JD provided novel insights into molecular mechanisms associated with immune system responses to MAP infection in dairy cattle.

Ways of identifying lichen and plant species by the Nenets reindeer herders in Yamal

Yamal Nenets herders have historically developed a rich knowledge of lichens and vascular plants, which feature in the diet of their migratory reindeer herds in the tundra zone of northwest Siberia. In the Nenets language there are native names for certain species of lichens and other reindeer forage plants, including graminoids, herbs, shrubs, berries, and mushrooms. During participant fieldwork together with nomadic tundra Nenets herders, we documented names and definitions of reindeer food on herding territories during their long migration routes from the northern forest-tundra transition zone to the northern coastal tundra. Like many other Indigenous peoples of Siberia, Nenets have noticed that the Arctic is changing and some of its recent dynamics are seriously affecting their livelihood. The degradation of some lichen composition and cover on tundra pastures has also contributed to a decrease of herders’ linguistic palette for describing these losses in a concrete manner. Since the Nenets language is on the list of endangered languages of the world, this has an especially negative impact on the language skills and traditional knowledge of the younger generations of Nenets people, who may not know what these lichens look like and why they are important for the Nenets reindeer herding culture.

Government’s failure to protect its citizens against nomadic herders’ aggression: a tacit permission for self-defense in Plateau State, Nigeria

PurposeThis paper aims to present the continuous Nigerian Government’s failure to protect the lives and property of its citizens against the incessant itinerant herders’ violence, despite its numerous programs in attempts to end the carnage. It sought also to examine the relationship between this government’s failure to meet its responsibility and the ineluctable self-defense mechanisms adopted by the people of Plateau State, Nigeria.Design/methodology/approachThe research was both quantitative and qualitative. The study was conducted in four of the 17 Local Government Areas of the state: Bassa, Jos-south, Riyom and Barkin Ladi. A sample size of 400 was determined using Yamane Taro’s sampling size formula. Four hundred respondents were interviewed using a Google questionnaire (found at this link: https://forms.gle/tu96ZDwP85e8JsGu8). In this study, a total of seven key informant interviews and nine focus group discussions were conducted.FindingsThe finding revealed that most indigenous ethnic groups were dissatisfied with the government’s handling of the nomadic herders’ aggression. Therefore, 99.1% of Berom, 99.0% of Irigwe and 92.9% of other ethnicities argued that the government’s failure to protect them is a tacit permission for self-defense. On the contrary, 60.0% of the Fulani were satisfied with the government’s strategies in ending the aggression and 95.0% of them argued that the government’s failure to protect its citizens is not an implied permission for self-defense. It was also found that a relationship exists between the government’s lack of capacity to end the nomadic herders’ aggression and implied consent for self-defense in Plateau State, Nigeria.Originality/valueThis is a research paper that uses primary data. The findings are germane to ending the challenge of recurrent aggression of nomadic herders on other Nigerians. The study concludes that the government must live up to its responsibility of the protection of its citizens’ lives and property, failure to do so is an implicit permission to the citizens to defend themselves. It also recommended that the government should return displaced people to their communities.

Lameness in Cattle-Etiopathogenesis, Prevention and Treatment.

The aim of this review was to analyse the health problem of lameness in dairy cows by assessing the health and economic losses. This review also presents in detail the etiopathogenesis of lameness in dairy cattle and examples of its treatment and prevention. This work is based on a review of available publications. In selecting articles for the manuscript, the authors focused on issues observed in cattle herds during their clinical work. Lameness in dairy cattle is a serious health and economic problem around the world. Production losses result from reduced milk yield, reduced feed intake, reproductive disorders, treatment costs, and costs associated with early culling. A significant difficulty in the control and treatment of lameness is the multifactorial nature of the disease; causes may be individual or species-specific and may be associated with the environment, nutrition, or the presence of concomitant diseases. An important role is ascribed to infectious agents of both systemic and local infections, which can cause problems with movement in animals. It is also worth noting the long treatment process, which can last up to several months, thus significantly affecting yield and production. Given the high economic losses resulting from lameness in dairy cows, reaching even >40% (depending on the scale of production), there seems to be a need to implement extensive preventive measures to reduce the occurrence of limb infections in animals. The most important effective preventive measures to reduce the occurrence of limb diseases with symptoms of lameness are periodic hoof examinations and correction, nutritional control, and bathing with disinfectants. A clean and dry environment for cows should also be a priority.

Revealing bovine schistosomiasis in Malawi: Connecting human and hybrid schistosomes within cattle

In Malawi, the putative origin of a newly described Schistosoma haematobium-mattheei hybrid human schistosome was assessed upon a seminal molecular parasitological survey of cattle. Using miracidia hatch test (MHT) and carcass inspection at slaughter, mean prevalence of bovine schistosomiasis was 49.1% (95% CI: 43.7–54.6%) and 10.3% (95% CI: 6.0–16.2%) respectively, though significant spatial heterogeneity was noted. Approximately 2.0% of infected cattle, and only those from Mangochi District, shed S. haematobium-mattheei and/or S. haematobium in faeces. To quantify schistosome (re)infection dynamics, where a S. haematobium-mattheei hybrid was present, we undertook a novel pilot GPS-datalogging sub-study within a specific herd of cattle (n = 8) on the Lake Malawi shoreline, alongside a praziquantel (40 mg/kg) treatment efficacy spot check. At sub-study baseline, all GPS-tagged cattle had proven daily water contact with the lake. Each animal was patently infected upon MHT, with older animals shedding less miracidia. At one month review, whilst parasitological cure was 100.0%, from six weeks onwards, (re)infection was first noted in the youngest animal. By three-month review, all animals were patently (re)infected though only miracidia of S. mattheei were recovered, albeit in much lower numbers. To conclude, infection with S. mattheei is particularly common in cattle and demonstrates a previously cryptic burden of bovine schistosomiasis. Within Mangochi District, bovine transmission of both S. haematobium-mattheei hybrids and S. haematobium are now incriminated, with unequivocal evidence of contemporary zoonotic spill-over. Future control of urogenital schistosomiasis here in the southern region needs to develop, then successfully integrate, a One Health approach with appropriate mitigating strategies to reduce and/or contain bovine schistosomiasis transmission.

Glycyrrhizin alleviates BoAHV-1-induced lung injury in guinea pigs by inhibiting the NF-κB/NLRP3 Signaling pathway and activating the Nrf2/HO-1 Signaling pathway.

Varicellovirus bovinealpha 1 (BoAHV-1) is a significant pathogen responsible for respiratory disease in cattle, capable of inducing lung damage independently or co-infection with bacteria. The widespread spread of BoAHV-1 in cattle herds has caused substantial economic losses to the cattle industry. The pathogenic mechanisms of BoAHV-1 are often relevant to robust inflammatory responses, increased oxidative burden, and the initiation of apoptosis. Glycyrrhizin (GLY) is a small-molecule triterpenoid saponin compound obtained from the herb liquorice, which has a broad spectrum of pharmacological properties such as antiviral, anti-inflammatory, and antioxidant effects. Furthermore, GLY regulates lung physiology by modulating oxidative stress, inflammatory response, and cell apoptosis through interference with the NF-κB/NLRP3 and Nrf2/HO-1 Signaling pathways. However, the potential of GLY to mitigate lung injury induced by BoAHV-1 and its underlying mechanism remains unclear. Therefore, in this study, we investigated the protective effect of GLY against pulmonary injury induced by BoAHV-1 in a guinea pig model by reducing viral load and suppressing the inflammatory response, oxidative stress, and apoptosis. The results of this study demonstrated that GLY exerted a protective effect against BoAHV-1-induced lung injury in guinea pigs. Specifically, GLY reduced the levels of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and interleukin (IL)-8 in guinea pig tissues while suppressing the expression of Caspase-1. Additionally, GLY reduced BoAHV-1 load and the number of TUNEL-positive lung cells in guinea pig lungs while inhibiting Caspase 3 protein expression. Furthermore, GLY significantly enhanced lung antioxidant capacity by increasing superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity while simultaneously reducing malondialdehyde (MDA) levels. Lung histological observation and score further validated the protective effect of GLY on BoAHV-1-induced lung injury. Furthermore, we observed that the expression of phosphorylated NF-κB p65 (p-NF-κB p65) and NLRP3 proteins in the lung tissue of BoAHV-1-infected guinea pigs decreased after GLY treatment while the expression of Nrf2 and HO-1 proteins increased. These results indicated that GLY inhibited the NF-κB/NLRP3 Signaling pathway and activated the Nrf2/HO-1 Signaling pathway during BoAHV-1 infection. Ultimately, our findings demonstrated that GLY alleviates BoAHV-1-induced inflammation response, oxidative stress, and cell apoptosis by inhibiting the NF-κB/NLRP3 Signaling pathway and activating the Nrf2/HO-1 Signaling pathway to protect guinea pigs from lung injury caused by BoAHV-1. Ultimately, our findings demonstrated that GLY alleviates BoAHV-1-induced inflammation response, oxidative stress, and cell apoptosis by inhibiting the NF-κB/NLRP3 Signaling pathway and activating the Nrf2/HO-1 Signaling pathway to protect guinea pigs from lung injury caused by BoAHV-1. Importantly, this study provides a compelling argument for the GLY in combating respiratory disease in cattle caused by BoAHV-1.

Policies Affecting the Efficiency of Beef Production in Alberta: A Supply Chain Analysis

Shoppers face high beef prices at the supermarket, but those prices are not a reflection of what Canadian farmers and ranchers earn from their cow-calf herds. In the past 30 years, the average beef producer’s operating margin has never reached $50,000, despite the fact that the average beef farm’s asset base stands at more than $2 million. Better access to export markets, including the U.S., South Asia and North Africa, would help to remedy the producers poor returns. Export prices would need to cover production costs, the largest of which is feed for the producers’ cattle herds, accounting for 77 per cent of the average ranch’s cash costs. As of July 2023, Alberta’s herd consisted of 1.77 million beef and dairy cows. With demand for livestock-derived food expected to jump by 38 per cent in the next 30 years, Canadian cattle ranchers need to take advantage of this global increase through freer trade. Canadian beef can remain competitive globally if the supply chain accesses world markets beyond the U.S., especially in developing countries where consumer incomes are increasing. The industry also needs investments in research, farm extension and supply chain co-ordination from national and provincial self-funded producer groups. Producers must look outward to global trade but be ready to capture new innovations at home. The dominant economies of scale are available to beef processors and finding savings is difficult for farmers and ranchers. However, there is potential for the supply chain to see savings from new technology, which is why investment in continuing support for ranch-level production research is necessary. Producers also need to focus on national co-ordination aimed at protecting trade access and responding to trends in consumer demand for beef. Any new industry policies must also consider key factors that currently affect market demand and expansion including: changing consumer preferences globally, the welfare of animals raised for slaughter and the effects of greenhouse gas emissions on supply chain sustainability. As some of the output and byproducts of the grain production sector provide feed for cattle, policies meant to support the grain sector may be indirectly influencing the beef sector significantly — for good and bad. Infrastructure required for worker safety, animal welfare improvements or improved food safety also adds to the cost of the beef supply. Protectionist trends and increased tariffs pose a threat to the supply chain because they too can create new costs. This is why access to foreign markets is crucial for producers, along with continued investment in research, sector-wide co-ordination to support market access and reviewing crop support to ensure livestock producers are compensated if grain policy changes harm them. Although live animals and much processed Canadian beef are exported to the U.S., fostering good trade relations in Asia and Africa is vital, given the growth in incomes and consumer demand for beef that is predicted for those regions. Free trade is the basis of good agriculture policy and any move towards protectionist policies and higher tariffs is the biggest threat for new costs in the supply chain. Canada’s beef sector requires low-cost access to foreign markets, making free trade policy the single most important policy focus for the sector.

WPŁYW ZRÓZNICOWANEJ ZAWARTO ˙ SCI BIAŁKA I MOCZNIKA W MLEKU, JAKO BIOMERKERÓW ´ ENERGETYCZNO-BIAŁKOWEGO ZBILANSOWANIA DAWEK POKARMOWYCH, NA POZIOM WYBRANYCH CECH UZYTKOWO ˙ SCI MLECZNEJ KRÓW RASY POLSKIEJ ´ HOLSZTYNSKO-FRYZYJSKIEJ

The aim of the study was to assess the impact of varied levels of milk protein and urea content on selected milk production traits of Polish Holstein-Friesian cows. The research included 34,397 data sets related to daily milk production of Polish Holstein-Friesian cows. These animals were kept in 15 cattle herds located in the Mazovian and Podlasie voivodeships. In the first stage of the research, the animals were differentiated based on the level of urea in their milk and the percentage of protein. According to the adopted assumptions, the energy and protein balance level of feed rations was determined based on the concentration of urea and protein in the milk. Due to the level of urea, three cow groups were distinguished: &lt;150, 150–250, and &gt;250 mg · L-1. Depending on the concentration of protein in the milk, the following three groups of animals were determined: &lt;3.2%, 3.2–3.6%, and &gt;3.6%. The assessment of the impact of varied energy and protein balance levels in feed rations on selected milk production traits of cows was conducted in subsequent lactation periods, production level and fat to protein (F/P) groups. Four lactation periods were distinguished, which covered successive months of lactation: 1–3, 4–6, 7–10, and 11–18, three production groups, determined based on the following levels of daily milk yield: &lt;20, 20–30, and &gt;30 kg and as well three groups of cows with different F/P ratio in milk were distinguished: &lt;1.2, 1.2–1.6, &gt;1.6. In the next stage of the study, a detailed assessment was conducted on the impact of varied levels of energy and protein balance in feed rations on the analyzed milk production traits. The analyzed population of cows was divided into 9 groups based on the level of urea content (mg · L-1) and protein concentration (%), with varying levels of coverage of the protein and energy requirements of cows. The interdependencies between milk performance traits of cows fed diets with balanced levels of energy and protein and their peers, for whom the nutritional diets were differentiated based on energy-protein balance, were determined using Pearson correlation coefficients and linear regression. When assessing the energy supply of cows, the study showed that too low energy levels in diets lead to a decrease in the chemical composition of milk, while too high levels lead to a decrease in milk yield compared to peers fed optimally balanced diets. Milk from cows with inadequate energy and protein nutrition contained less fat and protein by 0.54% and 0.59%, respectively, compared to milk from cows fed optimally. On the other hand, excessive energy supply was accompanied by an increase in fat (+0.66%) and protein (+0.57%) concentration with a decrease in daily milk yield (-4,1 kg). When evaluating the level of protein coverage for cows, the study showed that an excess of protein in feed led to an increase in urea levels in milk. The level of urea in milk from cows fed with excess protein in their feed (&gt;250 mg · L-1 and &lt;3.2%; &gt;250 mg · L-1 and &gt;3.6%; &gt;250 mg · L-1 and 3.2–3.6%) contained more urea per liter compared to milk from cows fed a balanced diet, with increases of 127, 107, and 109 mg, respectively.