Abstract
Purpose. Study of the influence of polymorphism of GDF5 gene of Kalmyk cattle on exterior characteristics. Materials and Methods. The polymorphism of the GDF5 gene was examined in 60 individuals of Kalmyk cattle breed belonging to the APC “Plodovitoe” of the Maloderbetovsky district of the Republic of Kalmykia. DNA samples were isolated from whole blood collected from the jugular vein using an automated extraction method. Real-time polymerase chain reaction was performed to determine the T586C polymorphism of the GDF5 gene. Results. Among the studied herd of cattle, individuals with the TT genotype predominated - they accounted for 68.3% of the total. Animals with TC and CC genotypes accounted for 21.7 and 10.0%, respectively. The frequency of occurrence of the T allele of GDF5 gene was 0.792, which exceeded the frequency of the C allele (0.208) by 58.2%. The observed frequency of the TT and CC genotypes exceeded the expected ones by 5.6 and 5.7%, respectively. At the same time, the frequency of the TC genotype was lower than expected by 11.2%. Individuals with the TT genotype of the GDF5 gene were superior to their peers with the TC and CC genotypes in all measured parameters. Conclusion. TT genotype of GDF5 gene in the studied livestock of Kalmyk cattle breed, owned by the APC “Plodovitoye”, occurs with the highest frequency (0.683). A positive effect of the TT genotype of GDF5 gene on linear physique measurements of Kalmyk cattle was revealed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
