This work employs nitrogen plasma immersion ion implantation (PIII) to modify electrospinning polylactic acid membranes and immobilizes essential fibroblast growth factors(bFGF) by forming cross-linking bonds. The study investigates the modified membranes' surface characteristics and the stimulatory effects of cross-linked bFGF polylactic acid membranes on osteoblast and fibroblast proliferation. The PIII process occurs under low vacuum conditions and is controlled by processing time and power pulse width. The experimental results indicate that, within a 400-second N2-PIII treatment, the spun fibers remain undamaged, demonstrating an increase in hydrophilicity (from 117° to 38°/36°) and nitrogen content (from 0% to 7.54%/8.05%). XPS analysis suggests the formation of a C-N-C=O cross-linked bond. Cell culture and activity assessments indicate that the PIII-treated and cross-linked bFGF film exhibits significantly higher cell growth activity (P<0.05) than the untreated group. These intergroup differences are attributed to the surface cross-linking bond content. In osteogenic induction, the results for each day show that the treated group performs better. However, the intergroup disparities within the cross-linked bFGF group disappear with prolonged culture time due to the rapid osteogenesis prompted by bFGF. The findings suggest that PIII treatment of electrospinning polylactic acid membranes holds promise in promoting osteogenesis in bone tissue scaffolds.