Applying nitrogen fertilizer strategically enhances rice productivity in nitrogen-deficient soils. Splitting fertilizer doses across growth stages optimizes nutrient use efficiency. Monitoring chlorophyll levels correlates closely with leaf nitrogen content, crucial for assessing crop health. Non-destructive chlorophyll content meter techniques, such as the Chlorophyll Content Index (CCI), allow for precise nitrogen management adjustments in the field. However, its critical value may differ depending on crops and varieties. Hence, to find out the critical CCI value for rice cv. ADT 43, observational trials were conducted at the Experimental Farm of Annamalai University during two seasons Kuruvai (June-Oct 2023) and Navarai (Dec-March 2024). The pooled data of the present study indicated that the average LNC (maximum tillering, panicle initiation and booting stages) has a linear relationship (R2=0.8545) with the yield of rice crop. The corresponding higher yield ( greater than 7t/ha) coincided with LNC of 3.1%. Likewise, the average CCI has a linear relationship (R2=0.9031) with the LNC of rice plants. The corresponding LNC ( greater than 7t/ha) was matched with the CCI value of 29. This CCI value of 29 is considered as the critical CCI value. CCI can be used as a decision-support tool for N-fertilization of short duration rice crop, and when CCI reduced below this critical value the farmers can fertilise the field with adequate N.