You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 2015PD36-06 EFFICACY OF ROLIPRAM LOADED NANOSPHERES IN LOCALIZED DELIVERY TO SITES OF NERVE INJURY FOR PREVENTION OF POST-PROSTATECTOMY ERECTILE DYSFUNCTION USING A RAT MODEL OF CAVERNOUS NERVE INJURY Neal Patel, Amirali H. Salmasi, Michael Dinizo, Izak Faiena, Ritu Goyal, Geun Taek Lee, Johanna L. Hannan, Trinity J. Bivalacqua, Joachim Kohn, and Isaac Y. Kim Neal PatelNeal Patel More articles by this author , Amirali H. SalmasiAmirali H. Salmasi More articles by this author , Michael DinizoMichael Dinizo More articles by this author , Izak FaienaIzak Faiena More articles by this author , Ritu GoyalRitu Goyal More articles by this author , Geun Taek LeeGeun Taek Lee More articles by this author , Johanna L. HannanJohanna L. Hannan More articles by this author , Trinity J. BivalacquaTrinity J. Bivalacqua More articles by this author , Joachim KohnJoachim Kohn More articles by this author , and Isaac Y. KimIsaac Y. Kim More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2255AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Currently, there is no effective therapy to re-establish penile innervation and regenerate damaged cavernous nerves in post- radical prostatectomy (RP) patients. We previously reported potential neuroprotective mechanism of Phosphodiesterase type 4 inhibitors using cell culture data. We now explore the efficacy of Rolipram, a selective PDE-4 inhibitor, on post-nerve injury erectile dysfunction using our nanosphere (NSP) based delivery system to local sites of nerve injury. METHODS Rolipram loaded NSP which are tyrosine-derived copolymers that spontaneously self-assemble in aqueous media were produced. Twenty-four male Sprague Dawley rats were randomized into 4 groups. Three groups of rats received bilateral crush nerve injury (BCNI) and one group received no injury (sham, n=6). Of the rats that underwent BCNI, one group received no intervention (BNCI, n=6), while one group received rolipram loaded NSP over site of induced crush injury bilaterally (BCNI+Rolipram+NSP, n=6), and the last group was treated with empty nanospheres after BCNI (NSP, n=6). After 14 days, each rat underwent erectile function testing and immunohistochemistry of phalloidin, neurofilaments, and neuronal nitric oxide synthase in the penile dorsal nerve was performed on mid penile cross-sections. RESULTS Erectile function in rats treated with Rolipram loaded NSPs was significantly improved compared to the BNCI group (p<0.05). Phalloidin, neurofilament, and neuronal nitric oxide synthase quantified expression was significantly greater in Rolipram loaded NSP group compared to the BNCI group (p<0.05, Figure 1). CONCLUSIONS Immediate placement of a localized drug delivery system such as Rolipram loaded NSPs to the site of nerve injury showed improvement in erectile function. This therapeutic approach of localized drug delivery at the time of surgery would benefit a large number of post-RP patients and improve cavernous nerve regeneration. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e766-e767 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Neal Patel More articles by this author Amirali H. Salmasi More articles by this author Michael Dinizo More articles by this author Izak Faiena More articles by this author Ritu Goyal More articles by this author Geun Taek Lee More articles by this author Johanna L. Hannan More articles by this author Trinity J. Bivalacqua More articles by this author Joachim Kohn More articles by this author Isaac Y. Kim More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...