Abstract
ObjectiveThe present study attempts to compare the immunohistochemistry (IHC) of von Willebrand factor (vWf) , endothelial cadherin, Caveolin and endothelial Nitric Oxide Synthase (eNOS) in VasoView Endoscopic Vein Harvesting (EVH) versus traditional Open Vein Harvesting (OVH) techniques for Coronary Artery Bypass Graft (CABG) Surgery performed in Javad al Aemeh Hospital of Mashhad, Iran in 2013,.Methods and materialsForty-seven patients were scheduled for CABG (30 EVH and 17 OVH) among whom patients with relatively same gender and similar age were selected. Three separate two cm vein samples were harvested from each patient’s saphenous vein. Each portion was collected from distal, middle and proximal zones of the saphenous vein. The tissues were deparaffinized, and antigen retrieval was done using EZ-retriever followed by an immunohistochemistry evaluation with vWf, e-cadherin, Caveolin and eNOS. In addition, demographic questioner as of Lipid profile, FBS, BMI, and cardiovascular risk factors were collected. Data analyses, including parametric and nonparametric tests were undertaken using the SPSS 16 software. A P value < 0.05 was regarded as statistically significant.ResultsThe mean age of the EVH and OVH groups were 63.76 ± 9.51 and 63.63 ± 8.31 years respectively with no significant difference between them (p = 0.989). In addition, there was no great difference between the EVH and OVH groups in lipid profile, DM, HTN, smoking history, CVA, and valvular dysfunction (P > 0.05). Qualitative report of vWf, e-cadherin, Caveolin and eNOS reveals no significant difference between the EVH and OVH (P > 0.05).ConclusionThis study indicates that VasoView EVH technique causes no endothelial damage in comparison with OVH. This study could be a molecular confirmation for the innocuous of EVH technique.
Highlights
Many people around the world suffer from coronary artery disease (CAD) that causes an enormous morbidity and mortality
There was no great difference between the Endoscopic Vein Harvesting (EVH) and Open Vein Harvesting (OVH) groups in lipid profile, Diabetes mellitus (DM), HTN, smoking history, Cerebrovascular accident (CVA), and valvular dysfunction (P > 0.05)
Qualitative report of von Willebrand factor (vWf), e-cadherin, Caveolin and endothelial Nitric Oxide Synthase (eNOS) reveals no significant difference between the EVH and OVH (P > 0.05)
Summary
Many people around the world suffer from coronary artery disease (CAD) that causes an enormous morbidity and mortality. In order to rescue individuals with this disease there is the major and best operation/cardiac surgery known as coronary artery bypass grafting (CABG) [1]. This specified surgery is performed on individuals with each of followed diseases: 1) left main coronary artery disease, 2) 3-vessel disease, 3) 3-vessel disease in diabetics, 4). Endoscopic greater saphenous vein harvesting (EVH) decreases the wound complications related with open techniques [5]. In order to decrease the considerable morbidity and wound complications associated with the extensive incisions made in traditional approach to vein harvest, minimally invasive techniques such as EVH is recommended [6]. EVH uses CO2 and an endoscope to harvest the saphenous vein by a single tiny incision [9]
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