Net Protein Requirements Research Articles

Energy and protein requirements for maintenance and weight gain of Canindé goats supplemented on pasture in the semi-arid region

The purpose of this study was to estimate the carcass composition, and energy and protein requirements for maintenance and weight gain of Canindé goats supplemented on pasture in a semi-arid region. Forty-six castrated animals, with initial body weight (BW) of 15.7 ± 0.96 kg, grazing in Caatinga were used. Six animals were slaughtered at the beginning of the experiment representing the initial carcass composition (reference animals; 16.1 ± 1.15 kg BW), while the other animals were randomly distributed in four treatments (concentrate supplementation at 0%, 0.5%, 1.0%, and 1.5% BW), with ten animals per treatment. When one of the animals of each group reached 25 kg BW, all animals in that group were slaughtered. The whole empty body was weighed, ground, homogenized, and sampled for further chemical analyses. The level of supplementation influenced dry matter intake, retained energy, and body weight gain of Canindé goats. The energy (kcal/kg) and fat (g/kg EBW) contents in the carcass of goats of 15, 20, and 25 kg were 2.29 and 86.11, 2.39 and 113.48, and 2.47 and 140.53, respectively. The minimum nitrogen losses were 188 mg N/EBW0.75. The net and metabolizable energy for maintenance were 72.11 kcal/EBW0.75/day and 2.19 g/kg EBW0.75/day, respectively. The net and metabolizable protein requirements were 2.19 and 1.88 g/kg EBW0.75, respectively. The net energy and protein requirements for weight gain ranged from 2.61 to 2.81 Mcal/kg EBW gain and 194.37–222.55 g protein/kg EBW, respectively, for animals from 15 to 25 kg BW. It is concluded that the energy and protein nutritional requirements of Canindé goats supplemented on pasture in the semi-arid region vary according to body weight.

Protein requirements for pregnant dairy cows

We aimed to estimate the protein requirements of pregnant Holstein × Gyr cows. Sixty-one Holstein × Gyr (HG) cows were used, with an average initial BW of 480 ± 10.1 kg and 5 ± 0.5 yrs of age. Cows were divided into 3 groups: pregnant (n = 43), non-pregnant (n = 12), and baseline (n = 6). Baseline animals were slaughtered before starting the experiment to estimate the initial body composition of the remaining animals. Pregnant and non-pregnant cows received 2 diets: maintenance and ad libitum. Pregnant cows were slaughtered at 139, 199, 241, and 268 gestation days (GD). First, we used data only from non-pregnant cows to determine the requirements for maintenance and growth in adult cows. The requirements of metabolizable protein for maintenance (MPm; g/EBW0.75/d) were estimated using a linear regression between the metabolizable protein intake (MPI, g/d) and average daily gain (g/d), and the MPm was defined as the intercept divided by the average BW0.75. Net protein requirements for gain (NPg; g/d) were estimated by the first derivative of the allometric equation between final crude protein (CP) in the body (kg) and the final EBW (kg). The efficiency of use of metabolizable protein for gain (k) was calculated from the regression between the retained protein (g/EBW0.75/d) and the MPI (g/EBW0.75/d), and k was the slope of this regression. The MPI was estimated by combining microbial protein synthesis (purine derivatives) with the digestible rumen undegradable protein [(total protein intake minus RDP) × intestinal digestibility]. Second, an exponential model was used to fit the protein accumulation in the gestational components in the function of GD. The first derivative of that model was considered the net requirement for gestation (NPgest). The efficiency of protein utilization for gestation (kgest) was calculated by the iterative method using the equation: Δ = MPI - (MPm + NPg/kg + NPgest/kgest). The iteration was performed aiming at a zero deviation between observed MPI and MP estimated by the requirements determined herein. We obtained a value of 3.88 g/EBW0.75/d for MPm. The estimation of NPg can be calculated according to the following equation: NPg = 0.716 × (EBWopen-0.308) × EBGcorrected, where EBWopen is the empty BW (kg) for non-pregnant animals and EBGcorrected is the empty body gain (kg/d) corrected for the gestational component. The k was determined as 0.347. The net protein requirements for gestation (NPgest) were determined as NPgest (g/d) = 0.0008722 × exp(0.01784 ×GD) × (calfweight/35). The efficiency of using metabolizable protein for gestation (kgest) was 0.625. It is important to highlight that different methods of MP estimates should not be mixed and that the proposed method requires the estimate of microbial protein (estimate it via urinary estimates) and might be a limitation for practical application. In conclusion, new studies should be conducted to validate our results and the methodology adopted to determine protein requirements for pregnancy in dairy cows. Due to the pattern of protein accumulation in the gestational components, we suggest that exponential model to describe protein requirements for pregnancy for dairy cows.

A new protein requirement system for dairy cows

Accurate prediction of protein requirements for maintenance and lactation is needed to develop more profitable diets and reduce N loss and its environmental impact. A new factorial approach for accounting for net protein requirement for maintenance (NPM) and metabolizable protein (MP) efficiency for lactation (EMPL) was developed from a meta-analysis of 223 N balance trials. We defined NPM as the sum of the endogenous protein fecal and urinary excretion and estimated it from the intercept of a nonlinear equation between N intake and combined total N fecal and urinary excretion. Our model had a strong goodness-of-fit to estimate NPM (6.32 ± 0.15 g protein/kg metabolic body weight; n = 807 treatment means; r = 0.91). We calculated the EMPL as a proportion of the N intake, minus N excreted in feces and urine, that was secreted in milk. A fixed-EMPL value of 0.705 ± 0.020 was proposed. In a second independent data set, nonammonia-nonmicrobial-N and microbial-N ruminal outflows were measured, and the adequacy of the MP prediction (51 studies; n = 192 means treatments) was assessed. Our system based on the fixed-EMPL model predicted the MP requirement for lactation and maintenance with higher accuracy than several North American and European dairy cattle nutrition models, including the INRA (2018) and NASEM (2021). Only the NRC (2001), CNCPS 6.5, and Feed into Milk (2004) models had similar accuracy to predict MP requirement. Our system may contribute to improve the prediction for MP requirements of maintenance and lactation. However, most refined predictive models of intestinal digestibility for rumen undegradable protein and microbial protein are still needed to reduce the evaluation biases in our model and external models for predicting the MP requirements of dairy cows.

92 Maternal Nutrient Restriction of Primiparous Beef Heifers During Late Gestation Decreases Colostrum Yield and Reduces Calf Vigor

Abstract We hypothesized that late gestational nutrient restriction (NR) would reduce colostrum yield and impair offspring transition to postnatal life. Primiparous, fall-calving crossbred beef heifers (BW: 451 ± 28 [SD] kg; BCS: 5.4 ± 0.7) were individually-fed either 100% (control; CON; n = 12) or 70% (n = 13) of NASEM net energy and metabolizable protein requirements for maintenance, pregnancy, and growth from d 160 of gestation to parturition. Delivery duration was determined as minutes from first fetal feet expulsion to birth. Calves were reared naturally by their dams and monitored for times from birth to first sternal recumbency, attempt to stand, and stand; vigor scores (1 = very weak, 5 = extremely vigorous) were assigned at 2, 5, 10, and 20 min of age. Total colostrum from one rear quarter was collected pre-suckling. Calf rectal temperatures were recorded at 0, 6, 12, 24, and 48 h postnatally. Data were analyzed with the fixed effects of treatment (single point) or treatment, hour, and their interaction (over time, using repeated measures). Date of calving was a fixed effect; calf sex was included when P < 0.25. Heifers fed CON had greater (P = 0.04) colostrum weight and volume than NR. Although gestational nutrition did not affect (P = 0.72) calf birth weight, calves born to CON heifers had faster (P = 0.02) times to stand and tended to have faster (P = 0.09) times to attempt to stand. Calves from CON heifers had greater (P = 0.05) 20 min vigor scores. Rectal temperatures at 0 h were greater (P = 0.02) in CON calves, but at 24 h were greater (P = 0.04) in NR calves. These data indicate that heifers nutrient restricted during late gestation have reduced colostrum yield and less vigorous calves, which may influence postnatal calf survival.

525 Late-Breaking: Late Gestational Nutrient Restriction of Primiparous Beef Heifers Decreases Milk Yield and Pre-weaning Calf Growth

Abstract Fall-calving Hereford-SimAngus heifers (single-sired; BW: 451 ± 28 [SD] kg; BCS: 5.4 ± 0.7) bred to a single sire were individually-fed 100% (control; CON; n = 12) or 70% (nutrient restricted; NR; n = 13) of NASEM net energy and metabolizable protein requirements for maintenance, pregnancy, and growth from d 160 of gestation to calving. Post-calving, all females were limit-fed chopped tall-fescue hay supplemented to meet lactational nutrient requirements in Calan gates to constrain calves’ diets to milk only until d 147. Four-hour milk yields were measured 5 times; calf size was determined every 21 d. Data were analyzed with treatment, calving date, and calf sex (when P < 0.25) as fixed effects; day and day x treatment were included for milk yield. From calving to d 147, CON cows had greater (P ≤ 0.003) BW, BCS, and backfat, but NR cows gained more (P ≤ 0.004) BW, BCS, and backfat. Milk weight and volume was 15% less (main effect; P < 0.04) for NR cows. Calf size did not differ at birth (P ≥ 0.27). From d 42 to 147, calves born to NR dams had lower (P ≤ 0.02) BW. Heart, abdominal, and flank girths were less (P ≤ 0.07) for NR calves through d 147. Calf ribeye area tended to be less (P ≤ 0.11) in NR calves at d 42, 84, and 126. Nutrient restricted calves had less shoulder to rump length (d 63 to 147; P ≤ 0.08), shoulder height (d 63 to 126; P ≤ 0.02), cannon length (d 42 to 105 and 147; P ≤ 0.09), and cannon circumference (d 63 and 105 to 147; P ≤ 0.06). These data indicate that even when dams are fed to meet lactational requirements, milk yield and calf growth are reduced by late gestational nutrient restriction.

Energy and Protein Requirements for the Maintenance of Growing Male Sika Deer (Cervus nippon).

The objective of this study is to study the effects of dietary intake levels on energy metabolism, carbon (C), and nitrogen (N) balance and to determine the maintenance requirements of energy and protein for male sika deer during their growing period. A total of 16 1-year-old male sika deer with similar body weight (BW) (63.25 ± 2.42 kg) were selected, with four animals per feed intake level. The feeding levels of the four groups for deer were 40, 60, 80, and 100% of the recommended amount, respectively. The nutrient digestibility and methane production were measured through digestion trials and respiratory trials. A 4 × 4 Latin Square design was performed in a respirometry trial. The results show that the apparent digestibility of C and N gradually increased as the level of feed intake decreased. Furthermore, with a decrease in feed intake level, the metabolic energy intake (MEI), heat production (HP), and retained energy (RE) of male sika deer significantly decreased (P < 0.01). The requirements of metabolic energy for maintenance (MEm) and net energy for maintenance (NEm) of growing deer are 251.17 and 223.62 kJ kg−1BW0.75d−1, respectively, as estimated according to the logarithmic regression equations between HP and MEI. The net N requirement for maintenance (NNm) and net protein requirement for maintenance (NPm) of growing male sika deer based on the linear relationship between retained nitrogen (RN) and daily nitrogen intake (NI) were 251.8 mg kg−1BW0.75d−1 and 1.57 g kg−1BW0.75d−1, respectively. The NEm and NPm values obtained from this experiment fill the gap in net energy and protein requirements and serve as basic data for establishing the nutritional standards forsika deer breeding in China.

Determination of energy and protein requirements of preweaned dairy calves: A multistudy approach

The first studies concerning nutrient requirements for preweaned dairy calves were from the 1920s and 1930s; however, few studies were published in the following decades. We aimed to determine energy and protein requirements of preweaning Holstein and Holstein × Gyr dairy calves in a multistudy meta-regression. We used a database composed of individual measurements of 166 preweaned male calves (138 submitted to treatments and 28 used as the reference group) from 4 studies that used the methodology of comparative slaughter. Animals with less than 15/16 of Holstein genetic composition were considered crossbred Holstein × Gyr, whereas other animals were considered Holstein. Net energy requirements for maintenance (NEM) were determined by the regression between heat production and metabolizable energy intake (MEI). The metabolizable energy requirements for maintenance were calculated by the iterative method, and the efficiency of use of metabolizable energy for maintenance was obtained by NEM divided by the metabolizable energy requirements for maintenance. Net energy requirements for gain (NEG) were estimated using a regression of the retained energy (RE) as a function of empty body weight (EBW) and empty body gain (EBG). The efficiency of use of metabolizable energy for gain was estimated by the regression of RE as a function of MEI, but with partitioning the MEI into MEI from liquid feed and MEI from starter feed. Additionally, the effect of a liquid feed (milk or milk replacer) was tested on the slope of the regression. The metabolizable protein requirements for maintenance (MPM) were estimated using the intercept of the regression between the metabolizable protein intake (MPI) and average daily gain. The MPM was determined as the ratio between the intercept and the metabolic body weight. Net protein requirements for gain (NPG) were estimated by the regression between retained protein, EBG, and RE. The efficiency of use of metabolizable protein for gain was estimated by the regression of the retained protein as a function of MPI, but with partitioning the MPI into MPI from liquid feed and MPI from starter feed. Additionally, the effect of a liquid feed (milk or milk replacer) was tested on the regression slope. Breed did not influence any of the nutrient requirements' estimates. The NEM was estimated as 70.2 kcal/metabolic body weight per day. The efficiency of use of metabolizable energy for maintenance observed was 66%. The NEG was estimated by the equation NEG = 0.0901 × EBW0.75 × EBG0.9539. The efficiency of use of metabolizable energy for gain was estimated as 57.6, 49.3, and 41.2% for milk, milk replacer, and starter feed, respectively. The MPM was estimated as 4.22 g/EBW0.75 per day, and the NPG was determined by the equation: NPG = 30.06 × EBG + 70.98 × RE. The efficiency of use of metabolizable protein for gain was estimated as 71.9, 59.2, and 44.4% for milk, milk replacer, and starter feed, respectively. We concluded that no differences were observed in energy and protein requirements between Holstein and Holstein × Gyr crossbred cows. The efficiencies of use of metabolizable energy and protein are greater for milk when compared with milk replacer and starter feed. Therefore, we propose that the equations generated herein should be used to estimate energy and protein requirements of preweaned Holstein and Holstein × Gyr crossbred dairy calves raised under tropical conditions.

Metabolizable Protein: 2. Requirements for Maintenance in Growing Saanen Goats.

This study aimed to estimate the protein requirements for the maintenance of growing Saanen goats of different sexes from 5 to 45 kg of body weight (BW) using two methods and applying a meta-analysis. For this purpose, two datasets were used. One dataset was used to evaluate the effects of sex on the protein requirements for maintenance using the comparative slaughter technique. This dataset was composed of 185 individual records (80 intact males, 62 castrated males, and 43 females) from six studies. The other dataset was used to evaluate the effects of sex on the protein requirements for maintenance using the N balance method. This dataset was composed of 136 individual records (59 intact males, 43 castrated males, and 34 females) from six studies. All studies applied an experimental design that provided different levels of N intake and different levels of N retention, allowing the development of regression equations to predict the net protein requirement for maintenance (NPM) and the metabolizable protein (MP) requirements for maintenance (MPM) in Saanen goats. The efficiency of MP use for maintenance (kPM) was computed as NPM/MPM. The efficiency of MP use for gain (kPG) was calculated using the equation of daily protein retained against daily MP intake above maintenance. A meta-analysis was applied using the MIXED procedure of SAS, in which sex was considered a fixed effect, and blocks nested in the studies and goat sex were considered as random effects. The NPM did not differ between sexes, irrespective of the approach used. The daily NPM estimated was 1.23 g/kg0.75 BW when using the comparative slaughter technique, while it was 3.18 g/kg0.75 BW when using the N balance technique for growing Saanen goats. The MPM estimated was 3.8 g/kg0.75 BW, the kPM was 0.33, and the kPG was 0.52. We observed that the NPM when using the comparative slaughter technique in growing Saanen goats is lower than that recommended by the current small ruminant feeding systems; on the other hand, the MPM was similar to previous reports by the feeding systems. Sex did not affect the protein requirements for maintenance and the efficiencies of use of metabolizable protein.

Protein requirements for maintenance and growth of Corriedale lambs

The protein requirements of lambs for growth and fiber production can be breed specific. The Corriedale is a dual-purpose lamb breed, suitable for fleece and meat production; however, there is no information regarding the specific protein requirements for its growth and maintenance. In this study, we aimed to determine the protein requirements of castrated Corriedale lambs for maintenance, weight gain, and wool growth. Purebred Corriedale lambs with average body weights (BW) of 28, 33, and 38 kg were distributed into three respective trial groups. Their requirements of net protein for maintenance (NPm), metabolizable protein for maintenance (MPm), net protein for weight gain (NPg), net protein for wool growth (NPw), metabolizable protein for weight gain (MPg), metabolizable protein for wool growth (MPw), and their efficiencies for metabolizable protein use were determined. The NPm and MPm of the Corriedale lambs with 28–38 kg BW were 1.44 ± 0.58 g/d/kg0.75 and 3.07 ± 1.28 g/d/kg0.75 of their fasting BW, respectively, and the MP efficiency use for maintenance was 0.47. The NPg and NPw increased with the lambs’ weight gain (from 100 to 250 g/day) and ranged from 9.53 ± 0.001–21.8 ± 0.003 and 7.13 ± 0.038–24.4 ± 0.079 g/d, respectively, in the lambs with 28–38 kg BW. The partial MP efficiencies for the maintenance use of a fleece-free body and wool protein retention were 0.72 and 0.27, respectively. The MPg and MPw ranged from 13.2 ± 0.002–30.3 ± 0.004 g/d and 26.4 ± 0.14–90.4 ± 0.29 g/d, respectively, in the lambs with 28–38 kg BW and an average daily weight gain of 100–250 g. The findings of this study can help feeding systems to improve their protein requirement recommendations for Corriedale sheep in the future.

Performance, body composition and net requirements of protein and energy for weight gain in steers supplemented with or without addition of lipids

This research aimed to evaluate the performance, body composition and net requirements for protein and energy in grazing Nellore steers supplemented with or without addition of lipids. Twenty-eight steers, 301 ± 5.8 kg BW, were used in the experiment. The comparative slaughter method was used, with four steers used as references. The remaining 24 steers were randomly distributed into four groups, during 180 days, according to treatment: only Panicum maximum cv. Mombasa grass; Mombasa grass with concentrated supplementation based on soybean meal; Mombasa grass with concentrate containing lipids from soybean oil; and Mombasa grass with concentrate containing lipids derived from soybean grains. The total DMI of the steers fed only the pasture did not differ (P &gt; 0.05) from the average intake of the treatments with or without addition of lipids. There were no differences (P &gt; 0.05) between treatments for total daily gain, carcass and non carcass for protein, energy and fat. The requirements of protein and energy did not differ (P &gt; 0.05) among the treatments. When the pasture is of good quality, supplementation does not alter the body composition or the net requirements of protein and energy for weight gain. The supplementations with higher concentrations of lipids (oil and grain) were not enough to promote greater gains in fat and energy in carcass.

Genotype effects on energy and protein requirements in growing male goats

Goat genotype may alter the net energy and protein requirements for maintenance (NEm and NPm, respectively) and weight gain (NEg and NPg).This study was designed to investigate and quantify the effect of goat type on NEm, NPm, NEg and NPg, and quantify the net requirements for energy and protein for dairy, meat and indigenous growing male goats. For that, comparative slaughter studies were gathered and a meta-analytical approach was used. Two distinct databases were organized: one composed of 233 individual records from 11 studies of meat (n = 81), dairy (n = 97) and indigenous (n = 55) growing male goats weighing from 4.50 to 51.0 kg, to depict NEm and NPm; and another database composed of 239 individual records from nine studies of meat (n = 87), dairy (n = 97) and indigenous (n = 55) growing male goats weighing from 4.30 to 51.0 kg, to depict NEg and NPg. Our findings showed that NEm of meat goats was 8.5% greater (336 ± 10.8 kJ/kg0.75 of empty BW; EBW) than dairy and indigenous goats (310 ± 8.20 kJ/kg0.75 EBW; P < 0.05). Whereas, NPm was not affected by goat type (1.92 ± 0.239 g/kg EBW; P = 0.91). The NPg was 185.1 ± 1.82 g/kg of EBW gain for goats weighing 5 kg BW and 192.5 ± 4.33 g/kg of EBW gain for goats weighing 45 kg BW, and thus did not change across goat type (P = 0.12). On the other hand, NEg increased from 7.29 ± 0.191 to 11.9 ± 0.386 MJ/kg of EBW in male dairy goats, and from 7.32 ± 0.144 to 15.7 ± 0.537 MJ/kg of EBW in meat and indigenous growing male goats weighing between 5 and 45 kg BW. When body protein was used as a predictor in the allometric equation instead of EBW seeking to account for the degree of maturity, goat type differences disappeared; however, this predictor showed a high variation among individuals. In conclusion, energy and protein requirements for gain in distinct goat types reflect on body composition differences. Future research should focus on better understanding the maturity degree and its consequences in the energy requirement of growing male goats and better depict the goat type effect on it, as well as on the efficiency of utilization.

Development of an equation to predict net protein requirements for the growth of Zebu beef cattle

The accurate estimation of protein requirements for beef cattle is a key factor in increasing livestock profitability and decreasing the environmental impacts of excessive N excretion due to mismatching between assumed requirements and diet formulation. A meta-analysis was conducted to evaluate and validate a new equation to predict the net protein requirements for growth (NPg) of Zebu beef cattle. For the development of the new approach, a database of 552 observations comprised of bulls, steers, and heifers of different genetic groups (Zebu, beef crossbreed, and dairy crossbreed) was assembled. The new approach was evaluated and compared to current models devised by the international nutrient requirements system committees (Agricultural Research Council, 1980; Beef Cattle Nutrient Requirements Model, 2016; BR-CORTE, 2016) to predict NPg. The model evaluation was performed through the model evaluation system (version 3.1.16) using an independent data set (n = 177 observations). An equation was considered the best estimator of NPg if the following conditions were met: (1) the intercept and slope of the regression between ordinary residues and/or predicted NPg values must have been equal to zero and one, respectively; and (2) the greatest concordance correlation coefficient (CCC) and determination coefficient (R), and lowest mean squared error of prediction (MSEP) were attained. Based on the regression models of the observed v. predicted NPg of Zebu beef cattle, both the new approach and that of the ARC (1980) correctly estimated NPg, since the intercept and slope were not different (P > 0.05) from zero and one, respectively. Additionally, the new approach’s determination coefficient was the greatest and the closest to one. The fact that the new model achieved a higher CCC and lower MSEP than the existing models indicated its superior reproducibility and accuracy. The equations proposed by BR-CORTE (2016) and the BCNRM (2016) did not correctly estimate NPg in that the intercept and slope were different (P < 0.01) from zero and one, respectively. Thus, the equations proposed by the new approach and the ARC (1980) accurately and precisely estimated NPg and are recommended for Zebu cattle. Furthermore, the inclusion of equivalent empty BW (EQEBW) in the new approach improves the estimation of NPg. We suggest the use of the following equation to calculate NPg for Zebu beef cattle: NPg = 176.01 × EBG – 0.381 × EQEBW0.75 × EBG1.035 (R = 0.80 and CCC = 0.75); where NPg = net protein requirements for growth, EBG = empty body gain, and EQEBW = equivalent empty BW.

Energy and protein requirements of crossbred Holstein × Gyr calves fed milk with milk replacer containing increasing dry-matter concentrations

Context There is a lack of studies concerning the nutrient requirements of dairy calves, mainly, evaluating different genetic groups. Aims The objective was to quantify energy and protein requirements of dairy calves up to 60 days, testing the influence of genetic composition (Holstein or crossbred Holstein × Gyr) on these requirements. Methods The study involved 42 bull calves (3 days of age), including animals with less than 15/16 Holstein composition (considered crossbred) and animals with more than 15/16 Holstein pedigree (considered purebred). Six calves were slaughtered at the start of the experiment to estimate the initial body composition of the animals. Of the remaining animals, four formed the maintenance group (fed 3 L/day of raw milk), and the other 32 were distributed into four treatments, which consisted of 6 L/day of raw milk, with increasing DM contents of 13.5%, 16.1%, 18.2% and 20.4% respectively. The DM contents were corrected for adding milk replacer to the raw milk. All animals had free access to starter feed and water. Digestibility trials were conducted at 28 and 56 days of life, with total faeces collection being performed for 5 days and urine collection for a period of 24 h. At 60 days of life, the animals were slaughtered to determine their body composition. Key results Net energy requirements for maintenance and metabolisable energy requirements for maintenance were 57.6 and 86.8 kcal/(empty bodyweight, EBW)0.75.day respectively. The efficiency of utilisation of metabolisable energy for maintenance was 66%. Net energy requirements for gain (NEg, Mcal/day) can be estimated by the following equation: , where EBG is empty body gain (kg/day) and EBW is in kilograms. The efficiency of utilisation of metabolisable energy for gain was 27%. The metabolisable-protein requirement for maintenance was 3.22 g/EBW0.75.day. Net protein requirement for gain (NPg, g/day) can be estimated by the following equation:, where RE is retained energy (Mcal/day). The efficiency of utilisation of metabolisable protein for gain was 59.1%. Conclusions Genetic group does not affect energy or protein requirements of pre-weaned calves. The estimates presented here can be used to calculate nutrient requirements of pre-weaned calves aged up to 60 days. Implications Inclusion of milk replacer in the liquid feed had a negative impact on diet quality.

Energy and protein requirements of Holstein × Gyr crossbred heifers

Nutrient requirements in cattle are dependent on physiological stage, breed and environmental conditions. In Holstein × Gyr crossbred dairy heifers, the lack of data remains a limiting factor for estimating energy and protein requirements. Thus, we aimed to estimate the energy and protein requirements of Holstein × Gyr crossbred heifers raised under tropical conditions. Twenty-two crossbred (½ Holstein × ½ Gyr) heifers with an average initial BW of 102.2 ± 3.4 kg and 3 to 4 months of age were used. To estimate requirements, the comparative slaughter technique was used: four animals were assigned to the reference group, slaughtered at the beginning of the experiment to estimate the initial empty BW (EBW) and composition of the animals that remained in the experiment. The remaining animals were randomized into three treatments based on targeted rates of BW gain: high (1.0 kg/day), low (0.5 kg/day) and close to maintenance (0.1 kg/day). At the end of the experiment, all animals were slaughtered to determine EBW, empty body gain (EBG) and body energy and protein contents. The linear regression parameters were estimated using PROC MIXED of SAS (version 9.4). Estimates of the parameters of non-linear regressions were adjusted through PROC NLIN of SAS using the Gauss–Newton method for parameter fit. The net requirements of energy for maintenance (NEm) and metabolizable energy for maintenance (MEm) were 0.303 and 0.469 MJ/EBW0.75 per day, respectively. The efficiency of use of MEm was 64.5%. The estimated equation to predict the net energy requirement for gain (NEg) was: NEg (MJ/day) = 0.299 × EBW0.75 × EBG0.601. The efficiency of use of ME for gain (kg) was 30.7%. The requirement of metabolizable protein for maintenance was 3.52 g/EBW0.75 per day. The equation to predict net protein requirement for gain (NPg) was: NPg (g/day) = 243.65 × EBW−0.091 × EBG. The efficiency of use of metabolizable protein for gain (k) was 50.8%. We observed noteworthy differences when comparing to ME and protein requirements of Holstein × Gyr crossbred heifers with other systems. In addition, we also observed differences in estimates for NEm, NEg, NPg, kg and k. Therefore, we propose that the equations generated in the present study should be used to estimate energy and protein requirements for Holstein × Gyr crossbred dairy heifers raised in tropical conditions in the post-weaning phase up to 185 kg of BW.

Energy and protein requirements of woolless sheep under tropical conditions

The objective of this study was to determine the energy and protein requirements for maintenance and weight gain of growing woolless sheep and, in addition, to estimate the shrunk body weight (SBW) and empty body weight (EBW) of the animals according to their body weight (BW) in the fed state. Data from 150 animals from three comparative slaughter experiments with confined castrated sheep averaging 5 ± 0.96 months of age and similar BW (20.74 ± 2.99 kg) were used. Three models were evaluated to estimate EBW from SBW: linear, linear with intercept and non-linear. The following requirements were estimated: net energy for maintenance (NEm), metabolizable for maintenance (MEm), net energy for gain (NEg), efficiency of use of metabolizable energy for gain, metabolizable protein requirement for maintenance (MPm), net protein requirement for gain (NPg), and efficiency of use of metabolizable protein for gain (k). Fasting losses corresponded to 6.18% of BW and the better model to establish the relationship between SBW and EBW was nonlinear. The NEm and MEm were 70.44 and 109.34 kcal/kg EBW0.75/day, respectively. For NEg, the adjusted model was based on the ratio of the energy retained as a function of the EBW and the desired empty body weight gain (EBG). Considering two lambs weighing 15 kg and 30 kg with a daily gain of 100 g, the estimated NEg is 181 and 360 kcal/kg EBW0.75/day, respectively. The estimated kg was 0.407. For MPm, the value of 3.19 g/kg EBW0.75/day was estimated. The estimated NPg for animals of 15 and 30 kg, with the same gain rate was 13.13 and 13.61 g/day, respectively, which corresponds to the protein concentration in gain of 170 and 161 g/kg EBG, and efficiency of use of the metabolizable protein for gain ranging from 0.66 to 0.41. The nutritional requirements of woolless sheep did not follow the standards assumed by the international feeding systems, except for the energy requirements for maintenance and efficiency of use of the metabolizable energy for maintenance, which did not differ. The losses represented by the gastrointestinal tract (GIT) after fasting and slaughtering periods are higher in woolless sheep compared to those presented by the international feeding systems.

Estimation of the net energy and protein requirements for maintenance of male arctic foxes (Alopex lagopus) during the growth period1,2.

The maintenance requirements of net energy and net protein were assumed to represent the most accurate and important values totally for the animal's utilization. The objective of this experiment was to determine the net energy and net protein requirements for maintenance of growing arctic foxes. The experiments was evaluated using regression models estimated from data collected by means of indirect calorimetry, nitrogen balance trials, and digestion and metabolism experiments. Thirty-six growing arctic foxes (3 487 ± 261.7 g) at the age of 85 days were randomly assigned to four groups with 9 animals in each group. Arctic foxes were fed a complete formula diet at four intake levels (100%, or 80%, 60%, and 40% of feed requirements) from 24 July 2017 to 23 September 2017. Arctic foxes in each treatment were kept individually in respiration chambers after 1-d adaptation at day 2 for a 3-d balance trial and then at day 5 followed by a 3-d fasting period. The metabolizable energy intake (MEI), heat production in the fed state (HP), and retained energy (RE) of arctic foxes significantly decreased (P < 0.01) as the feed intake level decreased. Fasting heat production (FHP) of arctic foxes was not influenced by feed intake level (P > 0.05). The metabolizable energy maintenance requirement (MEm) and net energy maintenance requirement (NEm) estimated from the linear relationship between RE and MEI were 230 and 217 kJ/kg of body weight BW0.75/d, respectively. The MEm and NEm estimated by logarithmic regression of HP on MEI were 225 and 209 kJ/kg BW0.75/d, respectively. The net N maintenance requirement (NNm) and net protein maintenance requirement (NPm) estimated from the linear relationship between retained nitrogen (RN) and daily nitrogen intake (NI) were 179.6 mg/kg BW0.75/d and 1.123 g/kg BW0.75/d, respectively. It is concluded that NEm and NPm values obtained fill the net energy and protein requirements shortage, and provide the basic data for establishing the standard of nutrition demand of breeding arctic foxes in China.

Oscillating and static dietary crude protein supply: II. Energy and protein requirements of young Nellore bulls.

:The objective of this study was to evaluate whole body chemical composition and energy and protein nutrient requirements for maintenance and gain of Nellore bulls. Fifty young bulls, with an average age of 7 ± 1 mo and initial body weight (BW) of 260.0 ± 8.1 kg, were used in this experiment. Four bulls were used as baseline reference animals and were slaughtered at the beginning of the experiment. Four bulls were fed at maintenance (12 g dry matter [DM]/kg of BW), whereas 42 bulls were divided into six groups (n = 7/group) and were randomly assigned to the following dietary treatments 105 (low [LO]), 125 (medium [MD]), or 145 (high [HI]) g crude protein (CP)/kg DM, LO to HI (LH), LO to MD (LM), or MD to HI (MH) oscillating CP at a 48-h interval for 140 d. At the end of the experiment, bulls were slaughtered and samples of the whole body were collected. All samples were lyophilized, ground, and composed as percentage of component of empty body weight (EBW) from each bull. A power model was used to estimate carcass, non-carcass components, and gastrointestinal content of the shrunk body weight (SBW), and CP and water present in the empty body, whereas an exponential model was used to estimate adipose tissue and ether extract (EE) present in the EBW. Nonlinear regression equations were developed to predict heat production from metabolizable energy (ME) intake and retained energy (RE). The net energy requirements for maintenance and ME for maintenance were 77 and 122.75 kcal/EBW0.75/d, respectively. The efficiency of ME utilization for maintenance was 62.7%. The equation obtained for net energy for gain (NEg) was: NEg (Mcal/EBW0.75/d) = 0.0535 × EBW0.75 × EBG0.7131, where EBG is the empty body gain, and the efficiency was 24.25%. Net protein requirement for growth (NPg) was: NPg (g/d) = 227.372 × EBG – 19.479 × RE. There was a linear increase for carcass, CP, and water present in the EBW as the animal grew. The EE deposition exponentially increased as EBW increased.

Energy and protein requirements of crossbred Holstein × Gyr calves fed commercial milk replacer and amino acid supplement

This experiment aimed to estimate the energy and protein requirements for Holstein × Gyr calves up to 60 days of age fed with milk replacer and amino acid supplement. Fifty male calves were used, of which seven were randomly allocated into the reference group and slaughtered at 8 days of age, seven were randomly allocated into the maintenance group slaughtered at 30 days of age, and the 36 remaining calves were included in the experiment at 8 days of age and randomly supplied with four dietary methionine+cysteine:lysine ratios (RMCL; 44%, 48%, 52%, and 56%) through amino acid supplement added as 1 kg/day of milk replacer reconstituted at 13.8% of dry matter. Different RMCL were tested for the models, and there were no significant effects on energy and protein requirements. The net energy requirement for maintenance was 75.2 kcal/empty bodyweight (EBW)0.75.day, with an energy use efficiency for maintenance of 67.38%. The prediction equation of net energy requirement for gain (Mcal/day) was energy retained = 0.0879 × EBW0.75 × empty bodyweight gain (EBWG)0.7580, with an energy use efficiency for gain of 47.57%. The estimated requirements for metabolisable protein for maintenance were 4.83 g/EBW0.75.day. The estimated equation for net protein requirements for gain (g/day) was NPg = EBWG × 246.73 × EBW–0.1204, with a protein use efficiency for gain of 71.55%. The estimated requirements for energy and proteins were greater than the values reported for calves fed with milk. Milk replacers are less efficiently used by calves up to 60 days of age when compared with whole milk.

Net protein requirements and metabolizable protein use for growing ram lambs fed diets differing in concentrate level and roughage source

Two trials were conducted to determine net protein requirements and the use of metabolizable protein (MP) for growing ram lambs when fed diets with different concentrate levels and roughage quality. In a comparative slaughter trial, eighty-four ½ Dorper × ½ Santa Inês ram lambs (18.0 ± 3.3 kg of BW) were individually penned and divided into 2 diet groups differing in roughage source: low-quality (sugarcane bagasse) roughage (LQR) or medium-quality (coastcross hay) roughage (MQR). In each group, 7 lambs were randomly selected and slaughtered after a 10 days adaptation period (baseline). Other 21 lambs from each diet group were fed ad libitum and slaughtered at 25, 35, or 45 kg of BW, and the remaining 28 lambs (14 from each diet group) were submitted to 1 of 2 levels of feed restriction (70 or 50% of the ad libitum intake). Body N content and retention were determined. In the second trial, 6 ram lambs (44.3 ± 5.6 kg of BW) were kept in metabolic cages and used in a 6 × 6 Latin square experiment designed to determine N digestibility and microbial N synthesis, and to compute MP supply by the 2 diets and 3 levels of intake. Nitrogen intake and microbial N synthesis were greater for the animals fed the MQR diet than for those fed the LQR diet (P < 0.05). Neither the endogenous and metabolic N losses, nor the net protein requirements for growth differed between diets (P > 0.05). However, the proportion of MP used for both maintenance and growth was lower for the animals fed the MQR diet (P < 0.05), likely because of an excess of MP reaching duodenum of these animals. We concluded that a reduction of the concentrate level in diets for growing ram lambs, achieved by improving the quality of the diet roughage, allows a greater supply of MP and may contribute to reduce diet protein concentration.

Net protein and energy requirements for weight gain of Santa Inês and Morada Nova sheep

The objective of this study was to determine the body composition and the dietary protein and energy requirements for weight gain of Santa Inês and Morada Nova sheep. A total of 48 intact lambs (24 Santa Inês and 24 Morada Nova), with initial weights of 21.7 ± 1.2 kg and 20.8 ± 0.8 kg and ages of 170 and 190 days, respectively, were used. At the beginning of the experimental phase, six lambs of each breed were slaughtered to determine body composition. The remaining 36 animals were divided into six groups of six animals each, separated by slaughter weights (25, 28 and 31 kg) and genotypes. The comparative slaughter technique was used to determine the nutritional requirements. The respective net requirements for energy gain ranged from 3.07 to 3.42 and 3.40 to 4.08 Mcal/kg gain of empty body weight (EBW), while for protein gain, the requirements ranged from 140 to 132 and 113 to 102 g/kg of EBW gain for intact genotypes lambs weighing between 22 and 31 kg. The Santa Inês sheep had higher net protein but lower net energy requirements for weight gain than the Morada Nova sheep. The body composition and the net requirements of protein and energy gains of genotypes sheep differ from the values reported by the main international committees of nutritional assessments.