Depopulation Research Articles

The effectiveness of some rhizobacteria on Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae) in cucumber plants

In this study, the possibilities of using 3 specific rhizobacteria isolates for the control of Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nematoda: Meloidogynidae) in cucumber plants of Beith alpha cultivar were investigated in 2023. The variables of the climate chamber experiment were seedling and seed treatments of the specific bacterial isolates and the QST713 (Serenade®) commercial isolate, nematode (1500 J2/pot) and non-nematode treatments, as well as positive and negative control treatments. As a result of the study, when the rate of root galling on cucumber roots was determined according to the Zeck scale, the most successful treatment in decreasing root galling was the seedling treatment of KD29 isolate (2.64), while the highest rate of root galling was observed in the positive control (6.27). When the bacterial treatments were compared with the positive control, it was observed that all bacterial treatments had a decreasing effect on the number of egg mass. When the effects of the treatments on the reproduction rate of the J2 population were analyzed, it was found that seedling treatments of isolate KD238 (0.69) and commercial isolate QST713 (0.86) had a decreasing effect on the J2 reproduction rate in the soil. As a result of the laboratory experiment, it was determined that KD157, KD238 and KD29 isolates had 42.25, 33.98 and 27.77% mortality effect on J2s after 96 hours, respectively. However, especially considering the decrease in the J2 population in the soil, the amount of root growth and the decrease in the number of egg mass, these bacteria stimulate the induced systemic resistance (ISR).

Peripheral-central network analysis of cancer cachexia status accompanied by the polarization of hypothalamic microglia with low expression of inhibitory immune checkpoint receptors

While the excessive inflammation in cancer cachexia is well-known to be induced by the overproduction of inflammatory mediators in the periphery, microflora disruption and brain dysfunction are also considered to contribute to the induction of cancer cachexia. Hypothalamic microglia play a crucial role in brain inflammation and central-peripheral immune circuits via the production of inflammatory mediators. In the present study, we evaluated possible changes in excessive secretion of gut microbiota-derived endotoxin and the expression timeline of several inflammation-regulatory mediators and their inhibiting modulators in hypothalamic microglia of a mouse model of cancer cachexia following transplantation of pancreatic cancer cells. We demonstrated that the plasma level of lipopolysaccharide (LPS) was significantly increased with an increase in anaerobic bacteria, especially Firmicutes, in the gut at the late stage of tumor-bearing mice that exhibited dramatic appetite loss, sarcopenia and severe peripheral immune suppression. At the early stage, in which tumor-bearing mice had not yet displayed “cachexia symptoms”, the mRNA expression of pro-inflammatory cytokines, but not of the neurodegenerative and severe inflammatory modulator lipocalin-2 (LCN2), was significantly increased, whereas at the late “cachexia stage”, the level of LCN2 mRNA was significantly increased along with significant decreases in levels of inhibitory immune checkpoint receptors programmed death receptor-1 (PD-1) and CD112R in hypothalamic microglia. In addition, a high density of activated neurons in the paraventricular nucleus (PVN) of the hypothalamus region and a significant increase in corticosterone secretion were found in cachexia model mice. Related to the cachexia state, released corticosterone was clearly increased in normal mice with specific activation of PVN neurons. A marked decrease in the natural killer cell population was also observed in the spleen of mice with robust activation of PVN neurons as well as mice with cancer cachexia. On the other hand, in vivo administration of LPS in normal mice induced hypothalamic microglia with low expression of inhibitory immune checkpoint receptors. These findings suggest that the induction of cancer cachexia may parallel exacerbation of the hypothalamic inflammatory status with polarization to microglia expressed with low levels of inhibitory immune checkpoint receptors following LPS release from the gut microflora.

Comparison Study of a Thermal-Driven Microstructure in a High-Ni Cathode for Lithium-Ion Batteries: Critical Calcination Temperature for Polycrystalline and Single-Crystalline Design.

High-Ni layered oxide cathodes are promising candidates for lithium-ion batteries due to their high energy density. However, their cycle stability is compromised by the poor mechanical durability of the particle microstructure. In this study, we investigate the impact of the calcination temperature on microstructural changes, including primary particle growth and pore evolution, using LiNi0.88Mn0.08Co0.04O2 (N884), with an emphasis on the critical calcination temperature for polycrystalline and single-crystal designs in high-Ni cathodes. As the calcination temperature increases, the primary particles undergo a rectangular growth pattern while the pore population decreases. Beyond a certain critical temperature (in this case, 850 °C), a sudden increase in primary particle size and a simultaneous rapid reduction in the pore population are observed. This sudden microstructure evolution leads to poor cycle retention in N884. In contrast, single-crystal particles, free of grain boundaries, synthesized at this critical temperature exhibit superior cycle retention, underscoring the significance of microstructural design over crystalline quality for achieving long-term cyclability. Our study sheds light on the interplay between calcination temperature and microstructural evolution, proposing the critical temperature as a key criterion for single-crystal synthesis.

Supplementation of Alternanthera sissoo pellets on feed digestion, rumen fermentation, and protozoal population in Thai native beef cattle

The objective of this experiment was to study the effects of Brazilian spinach (Alternanthera sissoo) pellet (BSP) supplementation on rumen fermentation, protozoal population, and methane (CH4) estimation in beef cattle. Four male Thai native beef cattle, 3 years old, with an average bodyweight of 180 ± 5 kg, were randomly arranged in a 4 × 4 Latin square design. The cattle were supplemented (on-top) with four levels of BSP (2, 4, 6, and 8% dry matter intake (DMI), respectively). The roughage component, derived from rice straw, was fed at 40 % of DMI, while the concentrate diet was fed at 60 % of DMI. The result of the experiment demonstrated that BSP supplementation had no effect on the DMI, nutrient intake, or nutrient digestibility (p > 0.05). Rumen pH and ammonia-nitrogen concentration were not significant, while the average protozoal population linearly decreased (p = 0.002) with BSP supplementation. Mean blood urea-nitrogen concentration was linearly increased (p = 0.004) when increasing the level of BSP. Brazilian spinach pellet had no significant effect on total volatile fatty acids (TVFA), VFA profiles, and CH4 estimation (p > 0.05). Nitrogen balance was no different from the supplementation of BSP. The study indicates that Brazilian spinach pellet supplementation showed no noticeable effects on feed intake, rumen parameters, and nitrogen utilization; however, at 6–8% of DMI, there was a decrease in protozoal population, with no corresponding reduction in CH4 estimation.

Fertility and self-rated health of migrant women of childbearing age—an analysis of moderating effects based on socioeconomic status

BackgroundAs fertility rates continue to decline and negative population growth emerges, China has sequentially introduced encouraging fertility policies to raise fertility levels. The impact of fertility on women’s health remains inconclusive. It is essential to explore further the correlation between fertility and the health status of 113 million migrant women of childbearing age in China.ObjectiveTo investigate how fertility affects the health status of migrant women of childbearing age and determine if migrant women’s socioeconomic status plays a moderating role in this process.MethodsUsing a nationally representative dataset from the 2018 China Migrants Dynamic Survey (CMDS), we examined the effects of fertility on the self-rated health of migrant women of childbearing age. An ordinary least squares regression model with moderating effects was used for the empirical study, and robustness tests were conducted based on the ordered probit model and propensity score matching to address endogeneity.ResultsThe empirical results indicated that a rise in the number of children born significantly reduces the self-rated health of migrant women of childbearing age. An increase in years of schooling and household income can significantly mitigate the negative impact of childbearing on the health of migrant women. The robustness of the above results was validated through alternative models and propensity score matching (PSM) methods. The heterogeneity analysis revealed that fertility exerts a negative impact on the health status of migrant women with rural household registration and on the health status of inter-provincial and inter-city migrant women. Further investigation found that the occurrence of childbirth during migration and an increase in the number of girls significantly negatively impacted the health status of migrant women. In contrast, the increase in the number of boys did not show a significant effect. Improving the health of migrant women of childbearing age significantly positively impacted their future childbearing intentions.ConclusionsMigrant women of childbearing age bear the dual burden of migration and childbirth. Our findings showed the rise in the number of children born and the occurrence of childbirth during migration posed greater challenges to the health status of female migrants, particularly among those with lower socioeconomic status. Government and community efforts for enhancing health among migrant women of childbearing age are recommended.

Teotihuacan ancient culture affected by megathrust earthquakes during the early Epiclassic Period (Mexico)

Teotihuacan was one of the thriving cultures in the Mesoamerica pre-Hispanic times, located in the Central Valley of Mexico. The city-state was a dominant centre point during the Classic period and its influence affected other contemporaneous cultures. Around the year 550 CE, a continuous decrease in urban population and selective building destruction was noted, accompanied by widespread fire. The layout of the city is identified by an avenue that articulates the political-administrative and religious centres, with such significant and impressive buildings as the Pyramid of the Sun, the Pyramid of the Moon and the Temple of Feathered Serpent. A systematic analysis of building damage in the pyramids reveals several Earthquake Archaeological Effects, (EAEs) potentially related to seismic loading. A damage pattern compatible with a strong ground shaking was also identified in the west staircase of the Old Temple of the Feathered Serpent, in the first rows of the west staircase of the Adosada platform (New Temple), and in the Pyramid of the Sun. In total, five ancient earthquakes have been determined from the damage, dated from the Tzacualli cultural period (1–100 CE), to the Xolalpan – Metepec period (450–550 CE). Unfortunately, this methodology does not determine the earthquake source. Therefore, we consider the possibility that repetitive megathrust earthquakes (Mw > 8.5) from the Middle American Trench (Pacific coast) could be responsible for the spatial pattern of the building damage. This proposal does not conflict with other existing theories for the Teotihuacan abrupt collapse, considering that the sudden overlapping of natural disasters like earthquakes could increase internal warfare (uprising), and civil unrest.

The effect of land cover on the foraging behavior and pollen in the honey of the giant bee Apis dorsata in Sumatra

IntroductionApis dorsata, the common bee pollinator in tropical forests, is experiencing a population decrease due to several anthropogenic factors that lead to land cover changes and habitat loss. Land cover changes may alter their resource supply and foraging behavior. Our study aimed to determine foraging behavior and botanical origin using pollen of A. dorsata honey in two land cover types: plantationdominated landscape (PL) in Kampar (Riau) and forest-agriculture-dominated landscape (FL) in Kerinci (Jambi) Sumatra, Indonesia.MethodsWe observed two colonies of A. dorsata flight direction and flight activities in each land cover from 9 am–3pm. Honey was harvested from both nests of A. dorsata and the pollen in the honey was analyzed using acetolysis procedure. Vegetation analysis in both locations was conducted based on the flight directions of the giant honey bees.ResultsThe foraging data of A. dorsata showed a difference in the total number of bees between these two land cover types. The number of bees flying out and returning to the nest was higher in Kerinci than in Kampar, while high morning foraging activities were recorded in both land cover types. Furthermore, the foraging activity of the colonies in the PL landscape, i.e., flying out and returning to the nest with and without pollen, decreased at noon. The palynological results of the honey showed that in the PL landscape, pollen diversity was very low and mainly consisted of Elaeis gueneensis pollen (97%). Meanwhile, pollen types and concentrations were much higher in the FL than in the PL.DiscussionThis result suggests that A. dorsata exhibits a more varied foraging behavior in a diverse and heterogeneous landscape in Kerinci compared to a plantation-dominated habitat in Kampar.

The Establishment of the Agricultural Landscape of Central Sicily Between the Middle Neolithic and the Beginning of the Iron Age

The possible co-variation of human occupation and vegetation from the Middle Neolithic to the beginning of the Iron Age (7.5–2.8 ka BP) in Central Sicily in the context of the central Mediterranean between Middle and Late Holocene are analysed in this paper to provide new insights on Sicilian prehistoric demography. The demographic and economic trends during these millennia were reconstructed using archaeological, Accelerator Mass Spectrometry 14C dates, palynological, archaeobotanical, and zooarchaeological data from the northern, central, and southern sectors of Central Sicily through a diachronic comparison with variation in Arboreal Pollen, Anthropogenic Pollen Indicators, Olea-Juglans-Castanea pollen, microcharcoals, and Sporormiella from four pollen cores from sites in different ecosystems. A very significant spread of farming activities was found at the end of the Neolithic, together with an apparent demographic gap during the Middle Copper Age, and the emergence of agricultural landscapes at the end of the Copper Age associated with a striking increase in population. A combination of cultural and climatic changes during the late phase of the Bronze Age resulted in a subsequent overall decrease in population.

Labor market in rural areas of Akmola region of Kazakhstan: regional aspect

The goal is to identify the features and determine quantitative parameters of the regional labor market in the Akmola region. Methods – analytical and statistical industry information is systematized and summarized using economic-statistical, balance sheet and abstract-logical methods from open access sources and scientific literature. Results - the features were studied and characteristics of labor potential of the region were given, analysis of absolute and relative indicators characterizing the dynamics of region's population movement was carried out, which made it possible to identify the main trends in its change in recent years. Demographic factors affecting the formation of labor force are shown. The volumes of socio-demographic load were calculated. Conclusions - it has been established that proportion of village residents tends to decrease both in absolute value and in the structure of total number. The main criteria are population migration and decrease in its natural increase. The reduction in the number of economically active residents of rural areas is explained by negative balance of difference between people who have entered active age and those who have retired, as well as migration processes that have their own specifics. It is noted that the level of the region’s labor resources is directly related to demographic development of the given territory. The deterioration of the demographic situation has a negative impact on quantitative and qualitative dimensions characterizing the regional labor market. The youth segment remains rather unbalanced, the areas of employment of young specialists are closely related to low levels of income and labor productivity, the persistent gap between professional orientation of youth and the labor needs of enterprises becomes one of the barriers to the employment of young people.

Evaluation of the Quality Changes in Three Commercial Pastourma Samples during Refrigerated Storage Using Physicochemical, Microbiological, and Image Analyses Combined with Chemometrics.

Despite the inherent stability of dried and cured products, such as pastourma, appropriate refrigeration remains essential for preserving their optimal characteristics. This study explored quality and safety characteristics in lamb, beef, and buffalo pastourma during 16-day refrigeration storage after package opening. The comprehensive approach employed Attenuated Total Reflection-Fourier-Transform Infrared (ATR-FTIR) spectroscopy, colorimetry, and image analysis, alongside physicochemical and microbiological analyses, to shed light on these alterations. The findings reveal a reduction in textural uniformity and color vibrancy (fading reds and yellows) across all samples during storage, with lamb pastourma exhibiting the most pronounced effects. Notably, image analysis emerged as a powerful tool, enabling the accurate classification of samples based on storage duration. Additionally, significant variations were observed in moisture content, hue angle, firmness, and TBARS levels, highlighting their influence on pastourma quality. The study documented a gradual decrease in lactic acid bacteria and aerobic plate count populations over time. ATR-FTIR spectra's interpretation revealed the presence of lipids, proteins, carbohydrates, and water. Protein secondary structures, demonstrably influenced by the meat type used, exhibited significant changes during storage, potentially impacting the functional and textural properties of pastourma. Overall, the findings contribute to a deeper understanding of pastourma spoilage during storage, paving the way for the development of improved preservation and storage strategies.

Abstract 5304: ABL407, a LILRB4x4-1BB bispecific antibody with a wild type Fc, exhibits potent antitumor activity by modulating immune system in multiple ways involving T cells, myeloids, and regulatory T cells

Abstract Tumor-associated myeloid cells such as myeloid-derived suppressor cells (MDSC) accumulate in the tumor microenvironment (TME) and suppress anti-tumor immune responses in a broad range of cancers. LILRB4 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 4), an emerging myeloid target, is specifically expressed in tumor-associated myeloid cells and suppresses the immune response, thus promoting cancer progression and metastasis. ABL407 is a First-in-Class bispecific antibody designed to simultaneously target LILRB4 and 4-1BB, enabling LILRB4 expression-dependent 4-1BB activation. By antagonizing LILRB4 signaling, ABL407 inhibited LILRB4 mediated immunosuppression, restoring T cell activity. In addition, ABL407 inhibited fibronectin, a potential LILRB4 ligand, -mediated suppression of myeloid cells. To evaluate the efficacy of ABL407, we established EL4 murine tumor model overexpressing LILRB4 (EL4/LILRB4) in h4-1BB transgenic mice and hLILRB1/4-h4-1BB triple transgenic mice. We observed LILRB4 was overexpressed in M-MDSC in the blood and liver-metastasized tumors. A significant decrease of M-MDSC population and tumor growth inhibition was observed in mice treated with anti-LILRB4 antibody and ABL407. ABL407 treatment resulted in a proportion increment of T cells. However, the percentage of Treg cells and M-MDSC in the liver and blood was reduced by ABL407 treatment in hLILRB1/4-h4-1BB triple transgenic mice. Mice with complete remission (CR) were further protected from the rechallenge of previously exposed tumor after 3 months of cessation of ABL407 treatment, implicating that immunological memory might be generated. In conclusion, our findings suggest that ABL407, LILRB4x4-1BB bispecific antibody, may be a promising strategy for the treatment of cancer. It can be applied as a "LILRB4 antagonist" as well as a "LILRB4-dependent 4-1BB agonist" across a broad spectrum of cancers. Citation Format: Minkyu Seon, Yangsoon Lee, Hyunseong Youn, Yelim Park, Sora Kim, Kyungjin Park, Jonghwa Won. ABL407, a LILRB4x4-1BB bispecific antibody with a wild type Fc, exhibits potent antitumor activity by modulating immune system in multiple ways involving T cells, myeloids, and regulatory T cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5304.

Prediction Of Population Size and Age Structure in China Based on Grey Prodiction and Leslie Model

Population is a major problem that restricts China's comprehensive, coordinated and sustainable development. Therefore, it is of great significance to establish a model that can accurately predict the population and age structure. This paper uses GM (1,1) to obtain the gender ratio of future newborn population and total population, quantifies the three-child policy as the increase in the fertility rate of women of childbearing age, uses the Leslie model to obtain the female population at all ages, and finally gets the national population and age structure in the next five years under the background of the three-child policy. The results show that the opening of the three-child policy positively impacts the increase of the newborn population and the growth rate of the total population in the early future. But as time goes by, it still cannot restrain the decrease in the newborn population and the net increase of the total population. In addition, the aging problem has not been effectively improved in the short term. In the next five years, the proportion of the aged population continues to rise, while the young population gradually decreases. In terms of the labor force coefficient, there will be a downward trend from 2021 to 2025 and a slight increase from 2025 to 2026.

Prediction Of Population Size and Age Structure in China Based on Grey Prodiction and Leslie Model

Population is a major problem that restricts China's comprehensive, coordinated and sustainable development. Therefore, it is of great significance to establish a model that can accurately predict the population and age structure. This paper uses GM (1,1) to obtain the gender ratio of future newborn population and total population, quantifies the three-child policy as the increase in the fertility rate of women of childbearing age, uses the Leslie model to obtain the female population at all ages, and finally gets the national population and age structure in the next five years under the background of the three-child policy. The results show that the opening of the three-child policy positively impacts the increase of the newborn population and the growth rate of the total population in the early future. But as time goes by, it still cannot restrain the decrease in the newborn population and the net increase of the total population. In addition, the aging problem has not been effectively improved in the short term. In the next five years, the proportion of the aged population continues to rise, while the young population gradually decreases. In terms of the labor force coefficient, there will be a downward trend from 2021 to 2025 and a slight increase from 2025 to 2026.

Effect of combination of essential oils on in vitro methane production, volatile fatty acid fractions and feed digestibility with goat rumen flora

Different combination of garlic oil (GO from Allium sativum L.), clove oil (CO from Eugenia spp.) and peppermint oil (P from Mentha piperita L.) as GO+PO (1:1), GO+CO (1:1), CO+PO (1:1) and GO+PO+CO (1:1:1) was evaluated in in vitro gas production test for their effect on total gas production, methane production, dry matter organic matter digestibility and volatile fatty acid fractions. The addition level was low (D1) at 0.25 g/L, medium (D2) at 0.5 g/L, and high (D3) at 1.0 g/L of fermentation medium. Concentrate mixture and bengal gram straw (Cicer arietinum L.) in 40:60 ratio was incubated with incubation medium containing goat rumen liquor for 24 h at 39°C in glass syringes. Three goats fed with concentrate pellet and Bengal gram straw served as donor of the rumen liquor. In vitro fluid pH varied between 6.50 to 6.59. Total gas production (mL/g DM) in control group was 154.07, at lower dose level (0.25 g/L) there was increase in gas production which significantly decreased at highest dose level. There was significant (P<0.05) decrease in the methane production (mL/g DM and mL/g DDM) on GO and PO supplementation together. At low dose level (D1, 0.25g/L) there was no statistically (P>0.05) significant difference in in vitro dry matter and organic matter digestibility with the addition of different EO combinations evaluated as compared to control except PO+ CLO (1:1). At dose level (D2 and D3) GO+PO combination led to a moderate decrease in the in IVDMD and IVOMD along with decrease in in vitro methane production. Fraction of propionic acid was higher on addition of garlic and peppermint oil together. There was relative decrease in the methanogens population with bacteria as housekeeping gene as expressed by RT-PCR amplification with GO+PO addition. Present study concluded that among the EOs combinations in vitro tested, garlic and peppermint oil combination (GO+PO) has better potential in reducing in vitro methane production with lower effect on in vitro digestibility.

Progress toward some of sustainable development goals in China's population-shrinking cities

A decrease in population is an emerging challenge in China's urbanization trajectory. This study utilizes data from the Sixth and Seventh Population Censuses to identify Chinese cities experiencing population decline, focusing on prefecture-level cities. It employs 30 indicators relevant to nine Sustainable Development Goals (SDGs) concerning urban areas and populations to evaluate the progress of these shrinking cities in 2010 and 2020. A novel metric, "SDG population elasticity", is introduced to quantify the association between population reduction and SDG indexes. The study employs K-means++ clustering for 130 shrinking cities. The findings reveal that out of the 130 cities, 63 exhibit a downward trend in SDG index scores, predominantly in China's northeast and western regions. The Northeast region has negative SDG population elasticity, indicating a lag in sustainable development compared to that in other areas. Additionally, the research categorizes China's shrinking cities into four groups: cities with strong population elasticity feedback-significantly declining SDGs, cities with weak population elasticity feedback-slightly regressing SDGs, cities with high population loss-negative SDGs in decline, and cities with smart population decline-steady SDGs growth. These findings offer a foundation for crafting bespoke policies in shrinking cities to ensure a balanced approach to sustainable development and monitor advancement toward achieving the SDGs.

Use of Carbon-Based Additives in Bio-Electrochemically Assisted Anaerobic Digestion for Cheese Whey Valorisation

This study explores the possibility of utilising electrochemically assisted anaerobic digestion supplemented with carbon-based materials to stimulate methanogenesis. Two different carbonaceous materials—commercial activated carbon (AC), and pyrolysed argan (PA, derived from argan shells)—were employed as supplements, with cheese whey (CW) being used as the substrate. Methane production slightly increased in the electrochemically assisted digesters, potentially translating into a 2–4% increase in the output of industrial digesters. In addition, reactors supplemented with PA also exhibited better production rates (496–508 L·kgVS−1), although there was no observed improvement in the quantity of biogas at the end of the biodegradability experiment. In contrast, when commercial AC was used as the supplement, the start-up phase was accelerated (5 days), although methane productivity decreased (273–352 L·kgVS−1). These observations were supported by microbiological analyses, demonstrating that the reactors with the poorest performance (those supplemented with AC) experienced the most significant decrease in both archaeal and bacterial populations.

Animal husbandry market in Kazakhstan: Dynamics and prognosis

Studies of livestock dynamics and productivity is an effective mechanism to monitor the possible risks to the breeding of animals of different species and a timely response to strengthen the food security of the Republic of Kazakhstan. The research aims to conduct an analytical review of the state of livestock breeding on the main species of animals that are bred in its territory and the forecast of development soon. The materials for the analysis were reports from the Bureau of National Statistics of the Republic of Kazakhstan, as well as information in Kazakh and foreign periodicals on the state of livestock breeding and current news in the agricultural sector of the country. The statistical materials on the number of animals of different species, gross output received from them, investment activities, and other indicators directly or indirectly related to the dynamic indicators in animal husbandry were used. The results of the analytical review revealed positive annual dynamics of increasing the number of livestock of most species of farm animals in the country. Thus, the growth of cattle population is 2-4% from year to year, a similar situation is observed in sheep (2-4%) and poultry (4-10%); the only exception is a decrease in pig population by 5-9%. Populations of cattle, sheep and goats, horses, and camels are mainly concentrated in small farms and individual households, so a significant part of products from them does not get to industrial processing, which leads to the need to import them to meet the needs of the urban population. The main mass of poultry, although represented by the livestock of specialized agricultural enterprises, the volume of output does not provide the necessary volume, which is reflected in an increase in purchases abroad. Therefore, to date, there are no visible prerequisites for reducing the number of animals of all species that are bred in Kazakhstan, and the state support of livestock breeding can contribute to the further growth of livestock

Carbonate microbialites and chemotrophic microbes: Insights from caves from south‐east China

ABSTRACTChemosynthetic microorganisms facilitate microbialite development in many caves throughout the world. In Youqin Cave and Tian'e Cave, located in the Carboniferous–Triassic carbonates on the South China Block, five Quaternary speleothems (stalagmite, stalactite and cave pearl) that are 2.3 to 11.0 cm long were examined for their petrographic, geochemical and microbiological features to reveal how chemotrophs contribute to microbialite growth. In the speleothems, millimetre‐sized stromatolites, thrombolites and calcified microbial mats are characterized by alternating light, calcitic microlaminae and dark, clay and organic‐rich calcite microlaminae. Filamentous (reticulate, smooth, nodular and helical), coccoid and bacilliform microbes, originally carried into the caves from surface soils, are more common in the dark microlaminae/clots than in the light microlaminae. 16S rRNA gene sequencing shows that the biotas in the microbialites are dominated by chemoorganotrophic heterotrophic bacteria, including primarily Sphingomonas, Crossiella and Acinetobacter, and rare Archaea. Diverse metabolic pathways of these prokaryotes, including ureolysis, denitrification and nitrite ammonification, contributed to increases in localized pH and/or dissolved inorganic carbon in these microenvironments, prompting carbonate precipitation. Development of the cave microbialites was probably controlled by the evolution of the cave microbial community as environmental conditions changed. Microbialite growth was probably mediated by the microorganisms that flourished on the speleothem surfaces during periods of low drip water rates and slow calcite precipitation. The change from microstromatolites to microthrombolites was probably linked to a decrease in cell populations in the microbial communities. These cave microbialites provide clear insights regarding the biogenicity and growth mechanisms of chemosynthetic microbialites. Given their association with chemolithotrophic activities that can date back to the Meso‐Archean, cave microbialites provide insights into the biogenicity and growth mechanisms of chemosynthesis‐based microbialites throughout geological history.

Length-Weight Relationship and Condition Factor Channa Marulioides (Bleeker, 1851) In Kapuas River – West Kalimantan as a Conservation Aquatic Resources Effort

Channa marulioides is one of the species that is facing a population decrease due to high fishing activity. This study aims to determine the value of the length-weight relationship and condition factors of C. marulioides in the Kapuas River as a relevant conservation effort. Several sampling areas in this study were used to represent the Kapuas River, including Kapuas Hulu River in Kapuas Hulu Regency, Putussibau District; The central part of the waters area of ​​Sintang Regency in Sintang Regency; and in the downstream waters of Pontianak City. The study carried out the sampling by analyzing the criteria for specimens with a dimension weight > 500 grams and length > 30 cm, with the same amount for each sex. The results showed that the growth type in the upper and middle waters was positive allometric with a value of b 3.1; however, it is negative allometric in the middle and upstream waters with b values ​​of 2.5 and 1.7. After research, the condition factor of the upstream and middle waters was observed to be in good condition with a value of 1. However, in the downstream waters the condition of the fish was rather bad with a value below 1, namely 0.99. The study concludes there is a decrease in the quality of the aquatic environment and the food availability from the middle waters to the downstream. This is what underlines conservation efforts in the form of domestication activities within the scope of structured fish farming.

Forecasting the Early Impact of COVID-19 on Physician Supply in EU Countries.

Many countries faced health workforce challenges even before the pandemic, such as impending retirements, negative population growth, or sub-optimal allocation of resources across health sectors. Current quantitative models are often of limited use, either because they require extensive individual-level data to be properly calibrated, or (in the absence of such data) because they are too simplistic to capture important demographic changes or disruptive epidemiological shocks such as the SARS-CoV-2 pandemic. We propose a population-dynamic and stock-flow-consistent approach to physician supply forecasting that is complex enough to account for dynamically changing behaviour, while requiring only publicly available time-series data for full calibration. We demonstrate the utility of this model by applying it to 21 European countries to forecast the supply of generalist and specialist physicians to 2040, and the impact of increased healthcare utilisation due to COVID-19 on this supply. Compared with the workforce needed to maintain physician density at 2019 levels, we find that in many countries there is indeed a significant trend towards decreasing generalist density at the expense of increasing specialist density. The trends for specialists are exacerbated by expectations of negative population growth in many Southern and Eastern European countries. Compared to the expected demographic changes in the population and the health workforce, we expect a limited impact of COVID-19 on these trends, even under conservative modelling assumptions. Finally, we generalise the approach to a multi-professional, multi-regional and multi-sectoral model for Austria, where we find an additional suboptimal distribution in the supply of contracted versus non-contracted (private) physicians. It is therefore vital to develop tools for decision-makers to influence the allocation and supply of doctors across specialties and sectors to address these imbalances.