The level of soil fertility plays a decisive role in the growth and development of crops and agricultural production. Reasonable fertilization time is of great significance for maintaining and improving soil fertility, increasing crop yield, and economic benefits. Rice is one of the main grain crops in China, and its yield and quality are influenced by natural environment, variety, water content, and fertility. Among them, fertility has the greatest impact on the yield and quality of rice. Within the scope of Design of experiments, rice yield is directly related to fertilizer application time. With the increase of fertilizer application time, rice yield increases significantly, and fertilizers and nutrient promoters play a good ecological and economic benefit. Although Biopesticide do not have a large yield increase effect, they can play the same effect as chemical pesticides. The amount of fertilizer used is the main factor in increasing crop yield, and the longterm application of nutrient promoters can also become an important factor in increasing yield. Different fertilization treatments also have different effects on the soil nutrient content of vegetable fields, and fertilization can significantly increase the soil nutrient content. This article takes rice as an example to study the effects of different fertilization times on rice yield and soil fertility changes.