Escherichia coli (E. coli) infects millions worldwide annually, leading to thousands of deaths, particularly impacting those low to middle-income countries where E. coli can be found in contaminated soil, water, and surfaces (Winstead et al. 2019). In this investigation, lavender essential oil and ampicillin disks were tested against E. coli grown on an agar plate to see if they could be used as a potential antimicrobial agent in regions where E. coli infections are largely prevalent. Based on the Kirby-Bauer tests conducted in this investigation, lavender essential oil displayed a clear zone of inhibition, though it was relatively smaller than the zone of inhibition for ampicillin. Future applications to such findings could be to utilize lavender in both plant and essential oil form as a treatment for E. coli soil contaminations and a natural pest repellent, both in the agricultural industry and in regions where cost-effective, environmentally sustainable treatments may not currently exist.