Natural Isotope Research Articles

Correction to: Natural uranium isotopes and 226Ra in surface and groundwater from a basin of a semiarid region in Brazil

Correction to: Natural uranium isotopes and 226Ra in surface and groundwater from a basin of a semiarid region in Brazil

Evaluating the Serum Carbon Isotope Ratio as a Biomarker for Animal Protein Ratio in a Controlled Feeding Study of US Adults

ObjectivesRecent studies have identified the serum natural abundance carbon isotope ratio (CIR) as a candidate biomarker of animal protein intake in postmenopausal women. Such a biomarker would help clarify the contribution of dietary protein source (animal vs. vegetable) to chronic disease risk. Here we evaluate biomarker performance and develop a biomarker calibration equation in a mixed-age and – gender cohort. MethodsWe conducted a 15-d feeding study of 100 adults (age 18–70, 55% women) in Phoenix, AZ. Participants were provided individualized diets that approximated habitual food intakes. Total CIR and nitrogen isotope ratio (NIR) were measured in sera collected at the end of the feeding period. We expressed animal protein as a ratio of total protein intake (APratio). We evaluated a model of serum CIR based on APratio, the serum NIR, gender, age and body weight, and the resulting regression equation was inverted to develop an equation for the APratio that we call the calibrated biomarker. We evaluated the association of the calibrated biomarker with actual APratio using Pearson correlation and 5-fold cross validation. ResultsAnimal protein intake in this study was 73 ± 30 g/d (mean ± SD) and the APratio was 0.63 ± 0.13. Our model explained a large proportion of the variation in serum CIR (R2 = 0.77) and APratio was the only significant model effect (coefficient = 6.22, SE = 0.44, P < 0.0001). Inverting that model generated the following biomarker calibration equation: APratio = (CIR – 26.35 – 0.06 (gender) + 0.068 * In age – 0.215 * In body weight – 0.204 * serum NIR)/6.22, where gender = 1,0 (male, female). There was a strong correlation between model-predicted and actual APratio (rP = 0.85, P < 0.0001), with the mean model-predicted APratio differing from mean actual APratio by 0.0015 (SE = 0.0077). The standard deviation of the prediction error was 0.076. The 5-fold cross validation procedure produced very similar model R2, effects, and prediction errors. ConclusionsThese data suggest that the serum CIR has potential as a predictive biomarker of APratio, providing a useful tool for objectively assessing dietary protein intake patterns. Such a tool could help resolve the contribution of dietary patterns favoring animal protein intake to chronic disease risk. Funding SourcesThis work was funded by NIH U01 CA197902.

Accumulation conditions and exploration directions of Ordovician lower assemblage natural gas, Ordos Basin, NW China

Based on drilling cores, well logging and seismic data, source rocks and reservoirs are evaluated; and the natural gas genesis is identified through the analysis of natural gas isotopes, components and fluid inclusions, to study the gas accumulation conditions of the gypsum salt rock related strata of the Ordovician lower assemblage in Ordos Basin. (1) The natural gas from Ordovician lower assemblage is high thermal evolution dry gas from marine source rock, characterized by relatively light δ 13 C value of methane and heavy δ 13 C value of ethane. The natural gas is identified as gas cracking from crude oil according to component analysis. Thermochemical sulfate reduction (TSR) reaction has happened between the hydrocarbon fluid and sulfate as sulfur crystals are found in the cores, hydrogen sulfide is found in the natural gas, and hydrocarbon and hydrogen sulfide fluid inclusions are widespread in secondary minerals. (2) Around the gypsum-salt lows, argillaceous rocks are extensive in the Ordovician lower assemblage, reaching a cumulative thickness of 20–80 m. The effective source rocks include argillaceous rock rich in organic laminae, algal clump and algal dolomite. Analysis shows that the source rocks have a dominant TOC of 0.1%–0.5%, 0.31% on average and 3.24% at maximum. The source rocks have an average TOC of 0.58% after recovered through organic acid salt method, indicating the source rocks have high hydrocarbon supply potential. (3) In the sedimentary period, the palaeo-uplift controlled the distribution of reservoirs. The inherited secondary palaeo-uplift in Wushenqi–Jingbian east of the central palaeo-uplift and the low uplift formed by thick salt rocks near Shenmu–Zizhou area controlled the distribution of penecontemporaneous grain shoal dolomite reservoirs. The salinization sedimentary environment of gypsum salt rock can promote the development of reservoir. There are three types of dolomite reservoirs, the one with intercrystalline pore, with dissolution pore, and with fracture; intercrystalline and dissolution pores are main reservoir spaces. (4) There are two types of cap rocks, namely tight carbonate rock and gypsum-salt rock, constituting two types of source-reservoir-cap assemblages respectively. The general accumulation model is characterized by marine source rock supplying hydrocarbon, beach facies limy dolomite reservoir, small fractures acting as migration pathways, and structural-lithologic traps as accumulation zones. (5) The third and fourth members of Majiagou Formation are major target layers in the lower assemblage. The Wushengqi–Jingbian secondary paleo-uplift area and Shenmu–Zizhou low uplift are dolomite and limestone transition zone, there develops tight limestone to the east of the uplift zone, which is conducive to the formation of gas reservoir sealed by lithology in the updip. Two risk exploration wells drilled recently have encouraging results, indicating that the two uplift zones are important prospects.

Groundwater Flow Model In The Center Of West Progo Dome, Kaligesing, Purworejo, Central Java And Its Surrounding Area, Based On Hydrochemical And Isotopic Characteristics

Groundwater studies were carried out in the center of the West Progo Dome, at Kaligesing, Purworejo District, Central Java, and its surrounding area, with an emphasis on hydrochemical problems. As a water-scarce area, groundwater studies are urgently needed in this area. This research is intended as a hydrogeological study with the aim of knowing the conceptual groundwater flow model in the study area. The method used is a field hydrogeological survey as well as hydrochemical and natural isotope analysis supported by chemical and groundwater isotope data. Less clear hydrochemical evolution indicates that the process of groundwater flow is dominant in the local flow system. Groundwater facies is dominated by bicarbonate type, neutral pH, relatively low total dissolved solid (TDS), and electric conductivity (EC), and influenced by season or rainfall. The dominant hydrochemical processes in the groundwater system are leaching, ion exchange, sulfate reduction, and dilution. Groundwater facies is determined by the rock minerals marked by differences in hardness and TDS. Whereas, stable isotope contents of groundwater vary from light to heavy. Springs with light isotopes show the circulation of deep groundwater flow or from a relatively high recharge zone, either locally or from other places around it. Isotopic enrichment in all seasons can occur due to evaporation or mixing with surface water that has undergone previous evapotranspiration, indicated by increasing of heavy isotopes or δD-excess (d) of groundwater. There are two types of groundwater flow patterns, namely shallow and deep groundwater flow patterns. Shallow groundwater is characterized by heavy isotopes, shifted with relatively small d. Deep groundwater circulation pattern is characterized by a consistent, light δD value and appreciable d.

Carbon and Nitrogen Sourcing in High Elevation Landscapes of Mustang in Central Nepal

Mustang valley in the central Himalaya of Nepal is a unique landscape formed by massive soil mass during a glacial period, which is attributed to a mix of vegetations and long agricultural history. Soil nutrients and their sourcing is highly important to understand the vegetation assemblage and land productivity in this arid zone. Twenty soil samples (from 0 to 20 cm depth) were collected from three landscape positions in Mustang district: valley, ridge, and midslope. We explored nutrient sourcing using natural abundance carbon (δ13C) and nitrogen isotope (δ15N) employing isotope ratio mass spectrophotometry. The results showed that the total soil carbon (TC) and total nitrogen (TN) ranged from 0.3 to 10.5% and 0.3 to 0.7%, respectively. Similarly, the CN ratio ranged from 0.75 to 15.6, whereas soil pH ranged from 6.5 to 7.5. Valley soil showed higher values of TN, CN, and soil pH than the ridge and midslope soils. The valleys had more positive δ15N signatures than ridge and midslope, which indicates higher inorganic and organic N fertilizer inputs in the valley bottom than in the midslope and ridge. This suggests that a higher nutrient content in the valley bottom likely results from agro-inputs management and the transport of nutrients from the ridge and midslope. Soil pH and CN ratio were a non-limiting factor of nutrient availability in the study regions. These findings are crucial in understanding the nutrient dynamics and management in relation to vegetation and agricultural farming in this unique topography of the Trans-Himalayan zone of Mustang in central Nepal.

Measurement of Snow Physical Properties and Stable Isotope Variations in the Canadian Sub-Arctic and Arctic Snowpack

ABSTRACT In northern Canada, the annual peak in river discharge is dominated by the seasonal input of snowmelt. As such, climatic changes that alter snowmelt properties and timing will have cascading impacts on the hydrological system as the Arctic warms. Geochemical tracers provide a tool to characterize the various processes governing the seasonal evolution of the snowpack; however, a lack of snow observations from a variety of Arctic landscapes limits the broad applicability of such tracers and further impedes our understanding of the various processes governing snowpack evolution and its ultimate contribution to the spring discharge peak. This study aims to gain a better understanding of the spatial distribution and the temporal evolution of the natural stable isotope signatures of snow from two distinct ecoregions: open tundra and taiga. More specifically, we describe the geophysical and stable isotope properties of the snow cover at Wekweètì (Northwest Territories), a high sub-Arctic taiga site, and within the Greiner Lake Watershed, near Cambridge Bay (Nunavut), an open Arctic tundra site. Results illustrate a link between snowpack formation and stable isotope distributions at both study sites. Stable oxygen isotope ratios of snow (δ 18O-H2O) show a wide range from −41‰ to −17‰ across all snow depth classes; however, heavy isotope enrichment is clearly visible in the bottom snow layers at both sites. Vapour flux from the ground under a strong temperature gradient is considered to be the main driver for this enrichment due to kinetic metamorphism, which is more prominent at the open tundra site. The stable isotope signatures of the bottom hoar layers during winter were found to be similar to river water values sampled during spring and summer, highlighting the need for more in-depth hydrological cycle assessment.

Competition of equilibrium and kinetic silicon isotope fractionation during silica precipitation from acidic to alkaline pH solutions in geothermal systems

As an important volcanic geothermal region in China, Tengchong belongs to the Mediterranean-Himalayan geothermal belt and is characterized by a wide distribution of geothermal systems. The study area- Rehai- is the sole geothermal area in Tengchong in mainland China that discharges both acidic and alkaline hot springs with pH values ranging from 1.6 to 9.7 at temperatures between 50 and 97 °C. Natural geothermal environments with large variations in pH and temperature are ideal study sites to investigate silicon (Si) isotope fractionation mechanisms which are still insufficiently understood. In this study, we investigated 3 different geothermal systems, which have distinct fluid characteristics: (1) type I fluid from Diretiyan Spring (pH: 1.6–2.5, HSO4 water, saturated with respect to quartz), (2) type II fluid from Zhenzhuquan Spring (pH: 6.4, NaClSO4 water, saturated with respect to quartz), and (3) type III fluid from Dagunguo Spring (pH: 7.8–9.7, NaCl water, oversaturated with respect to amorphous silica: SiO2am). The mechanisms controlling Si isotope fractionation during Si precipitation differ significantly between the three fluid types. In type I fluid, equilibrium fractionation controls Si isotope variability during silica precipitation, causing the highest natural Si isotope equilibrium fractionation (Δ30Si) detected to date, ranging in Δ30Siquartz-fluid from +1.09‰ to +2.78‰. The equilibrium isotope fractionation is essentially caused by balanced precipitation and dissolution rates and high ionic strength of the geothermal fluids. In type II fluid, amorphous silica formed during evaporation and/or cooling of the spring water during capillary rise and a kinetically controlled, uni-directional Si isotope fractionation is observed. In type III fluid, the Δ30SiSiO2am-fluid ranges from −1.57‰ to +0.55‰ at the temperature range of 80–100 °C. The Δ30SiSiO2am-fluid show a shift from kinetic to equilibrium controlled fractionation and demonstrate that isotopic re-equilibration can reset the original kinetic Si isotope values in dependence of the hydrochemical conditions like pH, temperature and ionic strength as well as reaction time. Here we show that isotopic re-equilibration after solid formation can shift the isotopic signatures originally inherited during precipitation, challenging the interpretation of Si isotope values in the ancient rock record.

Microstructure of bismuth centers in silicon before and after irradiation with 15 MeV protons

A decrease of two-gamma annihilation rate of a positron in a strong spin–orbit field of the annihilation site of bismuth impurity center 209Bi (J = 9/2) in silicon with natural isotope composition was revealed (J is the nuclear spin). This decrease was observed along with increasing occupancy of Bi donor states (binding energy E{Bi} ≈ 69 meV). Atoms of 29Si (J = 1/2) isotope are involved in spin interactions of positron with Bi impurity centers. The growth of occupancy of Bi donor states inhibits two-gamma annihilation rate. The estimated cross-section of positron trapping by the Bi impurity center is σ + ≈ (1.23–1.5) × 10−13 cm2. Together with this surprisingly large value, the integral rate of two-gamma annihilation in a hypothetical polyelectron system of the Bi impurity center is by a factor of just Δ ∼ 2.18 higher compared to the magnitude ≈2.09 × 109 s−1 known for elemental isolated polyelectron, (e−e+e−). Possible formation of the positron-containing exciton-like states, (e+)D 0 X (D = Bi, P) is also discussed. Irradiation of material with 15 MeV protons results in decreasing the factor Δ by ∼11% due to forming the radiation complex in which Bi atom is in an open volume ambient it. Such complex is suggested to have D 3d symmetry and be the deep donor. Low-temperature measurements of both the positron annihilation rate and Hall effect have been applied for studying the isochronal annealing of these point radiation defects which were found to be thermally stable up to ∼370 °C; they can be annealed at ∼430 °C – 470 °C. According to available data of ab initio cluster calculations, the complex of Bi atom with a simulated vacancy has D 3d symmetry with the energy gain ∼0.92 eV, thus indicating qualitative agreement between experimental and theoretical data.

In situ carbon and oxygen isotopes measurements in carbonates by fiber coupled laser diode-induced calcination: A step towards field isotopic characterization

Natural stable isotopes ratios (δ13Ccarb and δ18Ocarb) of carbonates archived in the geological record are routinely used to reconstruct local and global paleo temperatures and the secular evolution of the biogeochemical carbon cycle. The state-of-the-art technique, employed since the mid 20th century, to measure these isotopic ratios starts with field sampling followed by several steps of physical and chemical laboratory preparation including: (i) microdrilling and/or sawing and crushing, (ii) CO2 release by wet acid digestion, (iii) gas equilibration, purification and transfer, before (iv) gas phase IRMS measurements. While these steps are time and resource consuming, they provide accurate measurements of δ13Ccarb, δ18Ocarb and carbonate contents. This study presents a new protocol involving a compact and modernized laser calcination system that decreases drastically the analyses time by reducing the number of preparations steps together with offering the possibility of performing spatially resolved analysis at the mm scale. This new method is based on the use of a fiber coupled laser diode device emitting 30 W in the near infrared at 880 nm. The energy provided by the laser source induces the decomposition of calcium carbonate into lime and carbon dioxide. In this work, the CO2 was collected in sample tubes under a controlled atmosphere for offline analysis, however additional developments should permit online analysis in the near future.We analyzed 9 different types of carbonate minerals encompassing a range of isotopic compositions VPDB between +3.3 and − 18.2‰ and between −1.7 and − 14.6‰ for δ13Ccarb and δ18Ocarb, respectively. A comparison of isotopic results was performed for carbonate zones analyzed both by classic methods (micro-drilling followed by acid digestion) and laser calcination. This isotopic cross-calibration exercise shows a direct positive co-variation between both methods with a correlation coefficient of 0.99 and a regression slope of 1 within uncertainties for the δ13Ccarb values. The δ18Ocarb values also compared well with a correlation coefficient of 0.96, suggesting a constant gas-solid phase isotopic equilibrium between carbon dioxide and lime. The reproducibility of our laser calcination method performed on replicate analyses of dolomite, siderite and malachite shows a 1σ standard deviation of 0.31 and 0.77 for δ13Ccarb and δ18Ocarb, respectively. These reproducibilities are within the observed isotopic natural inhomogeneity of samples (up to 1.3 and 0.57‰ for the δ13Ccarb and δ18Ocarb, respectively) as assessed by microdrilling and acid digestion.Based on the suit of samples analyzed in this study, we demonstrate that (i) fiber coupled laser diode calcination enables accurate and reproducible C and O isotopic characterization of natural carbonates, (ii) physical effects during calcination do not introduce any isotopic fractionation for C and is accompanied by a constant isotopic offset for O over a range of isotopic compositions and mineral matrices. These findings pave the way for a new range of possibilities for carbonate δ13C and δ18O measurements directly in the field using rapid, portable, and easy to manipulate laser preparation devices paired with CRDS/IRIS optical-mass spectrometers.

Natural abundance isotope ratios to differentiate sources of carbon used during tumor growth in vivo

BackgroundRadioactive or stable isotopic labeling of metabolites is a strategy that is routinely used to map the cellular fate of a selected labeled metabolite after it is added to cell culture or to the circulation of an animal. However, a labeled metabolite can be enzymatically changed in cellular metabolism, complicating the use of this experimental strategy to understand how a labeled metabolite moves between organs. These methods are also technically demanding, expensive and potentially toxic. To allow quantification of the bulk movement of metabolites between organs, we have developed a novel application of stable isotope ratio mass spectrometry (IRMS).ResultsWe exploit natural differences in 13C/12C ratios of plant nutrients for a low-cost and non-toxic carbon labeling, allowing a measurement of bulk carbon transfer between organs in vivo. IRMS measurements were found to be sufficiently sensitive to measure organs from individual Drosophila melanogaster larvae, giving robust measurements down to 2.5 μg per sample. We apply the method to determine if carbon incorporated into a growing solid tumor is ultimately derived from food or host tissues.ConclusionMeasuring tumor growth in a D. melanogaster larvae tumor model reveals that these tumors derive a majority of carbon from host sources. We believe the low cost and non-toxic nature of this methodology gives it broad applicability to study carbon flows between organs also in other animals and for a range of other biological questions.

A search for rare and induced nuclear decays in hafnium

A measurement of hafnium foil using a modified ultra-low-background high purity detector with optimized sample-to-detector geometry was performed at Laboratori Nazionale del Gran Sasso. Radiopurity of the stock Hf foil was studied in detail, in addition to an analysis of data collected over 310 days to search for rare processes that can occur in natural Hf isotopes. Firstly, limits on alpha decays of all natural Hf isotopes to the first excited state of the daughter nuclides were established in the range of 1016–1018a (90% C.L.). Secondly, a search for modes of double electron capture and electron capture with positron emission in Hf174 was performed, yielding half-life limits 1016–1018a (90% C.L.). Lastly, novel dark matter-induced nuclear excitations in hafnium isotopes were investigated. For dark matter with 1 TeV/c2 mass, leading limits on the inelastic dark matter–nucleon cross section are set for mass splittings in the range 428 keV <δM< 473 keV in the adopted model framework.

Effects of Phyllostachys pubescens expansion on underground soil fauna community and soil food web in a Cryptomeria japonica plantation, Lushan Mountain, subtropical China

In order to determine the interactive effect of Phyllostachys pubescens (moso bamboo) expansion to adjacent coniferous forest (Cryptomeria japonica, Japanese cedar) on soil fauna communities and soil food web, we conducted a tangible investigation combined with natural abundance isotope analysis to examine how moso bamboo expansion process affects soil faunal composition and underground soil food web in Lushan mountain, southeast China. Exact treatments are as follows: (1) moso bamboo forest, (2) ecotone area and (3) Japanese cedar forest. We collected 74 arthropod groups from the field. The groups of Acari and Collembola were the two main soil fauna taxa with the highest abundance which accounted for 18.86–98.9% of the relative total abundance among various habitats. Peak of soil faunal density in moso bamboo and Japanese cedar forests appeared in May and November. Soil fauna community in ecotone was more similar to that in moso bamboo forest, indicating that the expansion process was still in infancy stage, and there was no significant difference in soil fauna community diversity index among the three forest types. Moso bamboo expansion did not affect the nutrient level of Collembola and Oribatida, but decreased that of Megsostigmata. The nutrient level of Hymenoptera and Coleoptera increased in ecotone, and Diptera kept in the third nutrient level in all three forest types, while the Hemiptera, Araneida and Pseudo-scorpionidea remained at a high level. The results demonstrated that in moso bamboo expansion process, soil fauna groups with low nutrient levels were more affected, while the soil faunas with high nutrient level were less affected.

Mechanistic investigation of Rh(i)-catalysed asymmetric Suzuki–Miyaura coupling with racemic allyl halides

Understanding how catalytic asymmetric reactions with racemic starting materials can operate would enable new enantioselective cross-coupling reactions that give chiral products. Here we propose a catalytic cycle for the highly enantioselective Rh(i)-catalysed Suzuki–Miyaura coupling of boronic acids and racemic allyl halides. Natural abundance 13C kinetic isotope effects provide quantitative information about the transition-state structures of two key elementary steps in the catalytic cycle, transmetallation and oxidative addition. Experiments with configurationally stable, deuterium-labelled substrates revealed that oxidative addition can happen via syn- or anti-pathways, which control diastereoselectivity. Density functional theory calculations attribute the extremely high enantioselectivity to reductive elimination from a common Rh complex formed from both allyl halide enantiomers. Our conclusions are supported by analysis of the reaction kinetics. These insights into the sequence of bond-forming steps and their transition-state structures will contribute to our understanding of asymmetric Rh–allyl chemistry and enable the discovery and application of asymmetric reactions with racemic substrates. A major drive in current chemistry research is to develop asymmetric versions of widely used carbon–carbon bond-forming reactions, such as Suzuki-Miyaura cross-couplings. Now, the origins of diastereo- and enantioselectivity in a Rh-catalysed cross-coupling of boronic acid and racemic allyl halides have been established.

Investigation on laser selective photoionisation of the 177mLu isomer

The selectivity of laser photoionisation of 177mLu nuclear isomer with respect to natural isotopes has been studied for the first time using a three-stage lutetium photoionisation scheme .

Updating the Fast Grain Boundary program: Temperature-time paths from intragrain oxygen isotope zoning

This contribution presents a rewritten and expanded version of the Fast Grain Boundary (FGB) program (Eiler et al., 1994) with the motivation of adding to the geochemical tools available for reconstructing temperature-time (T–t) histories that inform studies of tectonics and crustal evolution. Both the original and the new FGB programs model the oxygen-isotope compositional evolution of a rock resulting from diffusive oxygen isotope exchange between minerals. The new FGB program is coded in Python and includes a graphical user interface. Additionally, C-compiled versions of the code are available that provide a 20x speedup of model calculations. The new implementation also allows for inversion of the FGB model to extract unbiased thermal histories from oxygen isotope data. The Levenberg-Marquardt (LM) algorithm is applied to search for cooling histories that maximize agreement between the model output and the measured oxygen isotope data. Tests with synthetic datasets show that the LM algorithm is able to distinguish between simple linear cooling and more complex thermal histories that include reheating events. Inversion of a natural oxygen isotope zoning dataset from titanite shows that, within the resolution of the models and data, the Adirondack Mountains sample location experienced rapid (30–70 °C/m.y.), monotonic cooling from 700 to 500 °C. We develop a heuristic guide to sampling and analytical approaches that improve the resolution of inversion solutions for current SIMS analytical capabilities, and we suggest targets for future improvements of SIMS analysis. Our tests indicate that the current SIMS analytical precision for in situ oxygen isotope measurements is sufficient to allow for temperature-time path recovery with thermal resolution of 25–50 °C and temporal resolution of 2–3 million years.

Calculating radiation dose rate from online real-time environmental γ-spectrum using NaI(Tl) detector

Calculating gamma radiation dose rate from online real-time environmental gamma spectrum using NaI(Tl) detector has been developed into a software named RADAPROC V.1 in the Center for Operating the National Network of Environmental Radiation Monitoring And Warning (CONNERMAW). Currently, hundreds of online gamma spectra per day from online monitoring stations are processed to calculate the total ambient dose equivalent rate and the ambient dose equivalent rate of typical natural radioactive isotopes such as K-40, Bi-214, Tl-208 according to the method of using the function G(E) and the photo-peak area method. The calculated results have been compared with the results of calculating the dose rate from the specific activity of radioactive isotopes in soil samples collected at the same monitoring location and analyzed in the laboratory. The difference between the methods is less than 25%. The ambient dose equivalent rates of typical natural radioactive isotopes are a bit higher than those calculated with SARA-NMC software. The software will be improved shortly for better results.

Geochemical characteristics, origin, and mechanism of differential accumulation of natural gas in the carboniferous kelameili gas field in Junggar basin, China

Since the discovery of the Kelameili gas field, the exploration of volcanic gas reservoirs in the Junggar Basin has attracted considerable attention. However, owing to the limitations involved in studying the characteristics of the origin and distribution of the volcanic gas reservoirs in this area, the study of gas reservoirs in the South fault terrace area of the Kelameili gas field as a typical peripheral area is greatly restricted, and the exploration process is relatively slow. Therefore, it is necessary to analyse and compare the differences in the geochemical characteristics of the natural gas between the Kelameili gas field and the fault terrace area of the south belt by analysing the natural gas geochemistry, hydrocarbon generation evolution, and structural evolution. In addition, geochemical experiments involving the natural gas components, carbon isotopes, light hydrocarbons, hydrocarbon generation, and evolutionary history of the source rocks and the interpretation of seismic profiles reflecting the structural evolution, help clarifying the genetic type of the volcanic gas reservoirs and the source of the natural gas in this area. The results indicate the following. (1) The natural gas in the study area has undergone thermal genesis and is generated by kerogen cracking. It is a typical humic gas with high maturity. (2) The origin of the natural gas is the Carboniferous source rock in the depression, which is significantly different from the natural gas from the typical Permian and Jurassic. (3) The primary reason for the difference in natural gas accumulation is that the Carboniferous source rock entered the high-maturity stage in the Early Jurassic. The formation time of the trap is earlier than that of the high-maturity stage of the source rock evolution, which can capture multi-stage source rock products. (4) Based on the hydrocarbon source conditions that the hydrocarbon supply horizon of the Kelameili gas field has a high maturity of organic matter, and insufficient hydrocarbon supply, it is considered that traps capturing the multi-stage source rock products represent a larger scale of natural gas accumulation. The results of this study can deepen the understanding of volcanic gas reservoirs in the Junggar Basin and guidethe exploration and development of global volcanic gas reservoirs and the surrounding large-scale gas fields.

Nitrite isotope characteristics and associated soil N transformations

Nitrite (NO2−) is a crucial compound in the N soil cycle. As an intermediate of nearly all N transformations, its isotopic signature may provide precious information on the active pathways and processes. NO2− analyses have already been applied in 15N tracing studies, increasing their interpretation perspectives. Natural abundance NO2− isotope studies in soils were so far not applied and this study aims at testing if such analyses are useful in tracing the soil N cycle. We conducted laboratory soil incubations with parallel natural abundance and 15N treatments, accompanied by isotopic analyses of soil N compounds (NO3−, NO2−, NH4+). The double 15N tracing method was used as a reference method for estimations of N transformation processes based on natural abundance nitrite dynamics. We obtained a very good agreement between the results from nitrite isotope model proposed here and the 15N tracing approach. Natural abundance nitrite isotope studies are a promising tool to our understanding of soil N cycling.

Tracing plant-environment interactions from organismal to planetary scales using stable isotopes: a mini review.

Natural isotope variation forms a mosaic of isotopically distinct pools across the biosphere and flows between pools integrate plant ecology with global biogeochemical cycling. Carbon, nitrogen, and water isotopic ratios (among others) can be measured in plant tissues, at root and foliar interfaces, and in adjacent atmospheric, water, and soil environments. Natural abundance isotopes provide ecological insight to complement and enhance biogeochemical research, such as understanding the physiological conditions during photosynthetic assimilation (e.g. water stress) or the contribution of unusual plant water or nutrient sources (e.g. fog, foliar deposition). While foundational concepts and methods have endured through four decades of research, technological improvements that enable measurement at fine spatiotemporal scales, of multiple isotopes, and of isotopomers, are advancing the field of stable isotope ecology. For example, isotope studies now benefit from the maturation of field-portable infrared spectroscopy, which allows the exploration of plant-environment sensitivity at physiological timescales. Isotope ecology is also benefiting from, and contributing to, new understanding of the plant-soil-atmosphere system, such as improving the representation of soil carbon pools and turnover in land surface models. At larger Earth-system scales, a maturing global coverage of isotope data and new data from site networks offer exciting synthesis opportunities to merge the insights of single-or multi-isotope analysis with ecosystem and remote sensing data in a data-driven modeling framework, to create geospatial isotope products essential for studies of global environmental change.

Soil carbon dynamics of no-till silage maize in ley systems

Converting grassland to arable cropping leads to reduced soil organic carbon (SOC) stocks and thereby affects soil and climate protection goals. However, in forage production systems, silage maize produces considerable amounts of herbage and metabolizable energy yields for ruminants. To answer the question whether using direct drilling, as a minimal soil invasive technique, is able to prevent SOC stock degradation without loss of herbage yield in ley-arable systems, a two-year field experiment was set up on a sandy loam soil in northern Germany. In pre-management, a 10-year-old grassland sward was eradicated with a glyphosate and then sown with silage maize with (i) direct drilling (no-till, NT); and (ii) by conventional mouldboard ploughing (conventional tillage, CT). Each treatment included a non-N-fertilized (N0) and a 90 kg N ha−1 year−1 fertilized (N1) treatment. All silage maize treatments were analyzed in terms of above- (ANPP) and belowground net primary production (BNPP) and potential C input in comparison with the grassland control (GC) during the two years after conversion to arable silage maize. Quantification of SOC stocks and origin of C derived from residual plant material was estimated by the determination of natural 13C isotope abundance. Long-term impacts on SOC of a proposed ley-arable system (three years of grassland followed by three years of silage maize) using NT and CT practices were predicted using a soil C model. Results showed that ANPP and BNPP did not differ (p > 0.05) between tillage treatments and the two experimental years. Fertilization increased ANPP only in the second year with a lower fraction of roots belowground in comparison with N0 (p < 0.05). According to the 13C signature the total of silage maize-derived C after two years was up to 2.5–3.2 t C ha−1 in both tillage treatments, of which ∼76% can be explained by the measured C inputs from plant residues. The NT treatment showed lower amounts of decay of previously sequestered C in the upper 10 cm during the two-year period after land use change to silage maize. However, long-term predictions revealed ongoing breakdown of soil C regardless of tillage management used for continuous silage maize after conversion from grassland. The insertion of a three-years grass phase followed by three years of silage maize established with NT techniques was able to maintain SOC stocks in the long-term.