Natural Aging Process Research Articles

Revision Rhytidectomy: Pearls and Pitfalls.

While rhytidectomy can yield remarkable results, some patients may subsequently require revision rhytidectomy due to either unsatisfactory outcomes from primary surgery or the natural aging process many years later. Like most other secondary plastic surgical procedures, revision rhytidectomy is a complex undertaking and fraught with potential pitfalls. This can be attributable to natural or postsurgical alterations that can occur with the facial skin, superficial musculoaponeurotic system/platysma muscle, and/or facial nerve landmarks. Additionally, complications such as cobra neck deformity, pixie ear deformity, and synkinesis can add an extra level of complexity to revisional surgery. Furthermore, as more surgeons are performing deep-plane facelifts and more aggressive neck procedures, the risks are further amplified in revisional surgery. In this article, we review the challenges that a facelift surgeon may face when performing revision rhytidectomy and the technical considerations to overcome these obstacles.

EFFECT OF CRYOGENIC AND NATURAL AGING PROCESS APPLIED TO Al-Zn-Mg-Cu ALLOYS ON LIFE TIME CALCULATION

In this study, life time calculation of aluminum 7075 alloy with cryogenic and natural aging processes was performed by thermal analysis. The aluminum alloy was quenched after solid solution treatment at 480°C and naturally aged for 10-100 days at room temperature (25°C). Other samples were cryogenically treated at -40°C and -80°C for 2 hours after solid solution treatment at 480°C. After the cryogenic treatment, natural aging was done at room temperature for 10-100 days. At the end of each period determined for the samples, the hardness values were measured. It was observed that there was no significant change in hardness values at the end of 10 and 100 days at -80 °C. Thus, it was determined that the natural aging process does not start after cryogenic treatment at -80 °C. It was observed that the hardness value of naturally aged samples after cryogenic treatment at -40 °C increased more than the natural aged samples only. This showed that -40 °C improved the mechanism by creating a driving force in the material. Life time calculations between 30 °C and 320 °C also showed that -40 °C cryogenic treatment + natural aging increased life time by approximately 20% compared to natural aging alone.

EFFECT OF CRYOGENIC AND NATURAL AGING PROCESS APPLIED TO Al-Zn-Mg-Cu ALLOYS ON LIFE TIME CALCULATION

In this study, life time calculation of aluminum 7075 alloy with cryogenic and natural aging processes was performed by thermal analysis. The aluminum alloy was quenched after solid solution treatment at 480°C and naturally aged for 10-100 days at room temperature (25°C). Other samples were cryogenically treated at -40°C and -80°C for 2 hours after solid solution treatment at 480°C. After the cryogenic treatment, natural aging was done at room temperature for 10-100 days. At the end of each period determined for the samples, the hardness values were measured. It was observed that there was no significant change in hardness values at the end of 10 and 100 days at -80 °C. Thus, it was determined that the natural aging process does not start after cryogenic treatment at -80 °C. It was observed that the hardness value of naturally aged samples after cryogenic treatment at -40 °C increased more than the natural aged samples only. This showed that -40 °C improved the mechanism by creating a driving force in the material. Life time calculations between 30 °C and 320 °C also showed that -40 °C cryogenic treatment + natural aging increased life time by approximately 20% compared to natural aging alone.

Infraorbital Rejuvenation Combined with Thread-Lifting and Non-cross-linked Hyaluronic Acid Injection: A Retrospective, Case-Series Study

BackgroundInfraorbital aging develops during the natural aging process. Various treatment options offer unique benefits, accompanied by diverse side effect profiles, and can be synergistically combined to optimize results. This study aimed to evaluate the efficacy of a comprehensive approach involving non-cross-linked hyaluronic acid injection and smooth absorbable PPDO (poly p-dioxanone) thread insertion for infraorbital rejuvenation.MethodsThis retrospective case series study enrolled ten female patients with infraorbital aging from March 2022 to April 2023. Clinical outcomes, patient satisfaction, and adverse events were assessed at 1, 3, and 6 months posttreatment.ResultsThe median Global Aesthetic Improvement Scale scores evaluated by the operator and blinded evaluator were 1.70 ± 0.42 and 1.80 ± 0.35, respectively, at six months posttreatment. The median Allergan Infraorbital Hollows Scale determined by the operator was 1.15 ± 0.34 at six months posttreatment, whereas the scores evaluated by the blinded evaluator were 1.15 ± 0.53. At six months after treatment, 50% of patients were satisfied, and an additional 40% reported strong satisfaction with the clinical improvement following treatment. No serious adverse events, such as infections, lumps, irregularities, Tyndall effect, hematoma, or skin necrosis, occurred during the treatment period.ConclusionsThe combination of PPDO thread insertion and non-cross-linked hyaluronic acid injection yielded satisfactory and effective clinical outcomes with no occurrence of serious adverse events for infraorbital rejuvenation. We anticipate that this study will contribute to the advancement of novel treatment options for infraorbital aging.Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

Changes in physical performance after Fontan operation: a follow-up study

Abstract Background Maximal oxygen consumption (peak VO2) is markedly compromised in patients with Fontan circulation compared to healthy subjects. In addition to the natural aging process, adaptive mechanisms inherent to univentricular physiology may further contribute to a decline in peak VO2 over time. These patients may have limitations in their ability to engage in physical activity, which may exacerbate the decline in peak VO2. This study aims to quantify the impairment of functional capacity over time in patients in the late postoperative period of Fontan operation and the relationship with a sedentary lifestyle. Method We studied 46 patients, 57% female, with a median age of 28 (24 - 35) years and 17 (15 - 21) years after Fontan surgery. Maximum and submaximal variables were analyzed from two standardized cardiopulmonary exercise tests (CPET) on a treadmill and performed with a range of five (4 - 9) years between them. All patients' physical activity levels were ranked using the short version of the International Physical Activity Questionnaire (IPAQ), comparing the functional capacity between active and sedentary patients. Clinical and surgical data were extracted from medical records. We performed a descriptive analysis of the demographic, clinical, and surgical data and a comparative analysis between the variables of the first and second CPET. The annual change was calculated by dividing the differences between the last and first tests by the time interval between them. Maximal variables were analyzed only for patients who achieved a maximum test (respiratory exchange ratio was equal to or greater than ≥ 1.1, heart rate > 85% of the predicted, and signs of intense effort. Results Among the 46 patients in the study, 40 performed maximum CPET. The characteristics of patients are shown in Table 1. There was an annual decrease in peak VO2 of 0.42 (1.17- -0.71) mL/kg/min and 0.72 (-2.14 - 0.71) % of predicted VO2. Impaired submaximal parameters occurred in all patients. There was an increased number of sedentary subjects on the second test compared with the first (p=0.009) (Table 2). Comparing the functional capacity of inactive patients with active, we found lower values in peak VO2: 23.1 (20.8-25.1) vs. 35.1 (32.0-35.4) mL/kg/min (p<0.001) and in a percentage of predicted 62 (59-67) vs. 84 (76-97) % (p=0.012). When analyzing the ejection fraction of the systemic ventricle by most recent echocardiogram and brain natriuretic peptide (BNP) plasmatic level, these variables were not significantly changed. Conclusion Functional capacity decreased over time in the studied population, represented mainly by a significant annual loss in peak VO2 and the predicted age values. Reduced functional capacity was observed in the inactive patients compared with active. No change in ventricular function was identified, as well as in BNP plasmatic level over time.Table_1Table_2

The concept and development direction of elderly oriented food

Active response to the challenges posed by aging has been established as a national strategy, accompanied by continuous refinement of policies concerning aging work. Decreased digestive system function is commonly present in the natural aging process of older people, which directly affects their food intake, digestion, and absorption, leading to decreased appetite and various forms of malnutrition. It is also a risk factor for age-related diseases such as sarcopenia, osteoporosis, cognitive impairment, and frailty. Nutrition is the foundation for achieving healthy aging. In order to meet the nutritional needs of the elderly population, the Nutrition and Healthy Aging Working Group at the Shanghai Junshi Institute of Life Sciences introduced the concept of "elderly-oriented food" in 2022. In the future, there is an urgent need for comprehensive collaborative efforts to conduct a series of investigations focused on the dietary habits and nutritional requirements of older individuals, in order to evaluate the level of awareness, genuine demands, dietary preferences, and capabilities of selection regarding to the elderly-oriented food. Simultaneously, it is recommended to introduce "Guidelines for the production and application of elderly-oriented food" at the national level to promote the sustainable and orderly development in food industry.

Computational Analysis of the Influence of Menopause and Ageing on Bone Mineral Density, Exploring the Impact of Bone Turnover and Focal Bone Balance-A Study on Overload and Underload Scenarios.

This study aims to investigate the impact of hormonal imbalances during menopause, compounded by the natural ageing process, on bone health. Specifically, it examines the effects of increased bone turnover and focal bone balance on bone mass. A three-dimensional computational bone remodeling model was employed to simulate the response of the femur to habitual loads over a 19-year period, spanning premenopause, menopause, and postmenopause. The model was calibrated using experimental bone mineral density data from the literature to ensure accurate simulations. The study reveals that individual alterations in bone turnover or focal bone balance do not fully account for the observed experimental outcomes. Instead, simultaneous changes in both factors provide a more comprehensive explanation, leading to increased porosity while maintaining the material-to-apparent density ratio. Additionally, different load scenarios were tested, demonstrating that reaching the clinical osteoporosis threshold is independent of the timing of load changes. However, underload scenarios resulted in the threshold being reached approximately 6 years earlier than overload scenarios. These findings hold significant implications for strategies aimed at delaying the onset of osteoporosis and minimizing fracture risks through targeted mechanical stimulation during the early stages of menopause.

Genitourinary Syndrome of Menopause: A Narrative Review Focusing on Its Effects on the Sexual Health and Quality of Life of Women.

Genitourinary syndrome of menopause (GSM) is a progressive condition due to a hypoestrogenic state affecting perimenopausal and menopausal women. GSM was previously known as urogenital syndrome, vulvovaginal atrophy, or atrophic vaginitis. The term vulvovaginal atrophy did not encompass the symptoms of the urinary tract like incontinence, urgency, and discomfort, or allude that it is due to a hypoestrogenic state. Although a significant segment of the population is affected by GSM, it is very sparsely studied, detected, and treated. GSM affects the quality of life and sexual health of most menopausal women suffering from it. Only a few healthcare providers ask about the symptoms of GSM and a tiny percentage of women seek consultation for it. This may be because they are either embarrassed or believe it to be a part of the natural process of aging. As the life expectancy of women has increased in general, the prevalence of GSM has also risen, while it still remains underdiagnosed and untreated. Properly educating women so that they can seek consultation regarding symptoms of GSM, and training healthcare professionals about communicating with the patient, as well as correctly identifying, diagnosing, and managing the patient are all important to overcome this communication barrier.Once we cross the barrier of diagnosing patients with GSM, we still have to manage the patients with tailor-made prescriptions according to the severity of the symptoms and their preferences. While there are various treatment options, the most effective one is low-dose topical estrogen therapy. In this review, we intend to explore the existing knowledge about GSM and its effect on the quality of life and sexual health of women along with the treatment options for managing and reversing the effects of GSM.

Risky and non-risky financial investments and cognition

Much policy attention has been placed on encouraging saving behaviours to avoid financial deprivation at older adulthood. However, optimising financial investments is highly dependent on cognitive capacity, which can be deteriorated by the natural ageing process. This paper explores the relationship between age-related cognitive deterioration with risky and non-risky financial investments. The data analysed comes from eight waves (2002 to 2019) of the English Longitudinal Study of Ageing (ELSA) which, for a nationally representative sample, contains information about cognitive ability and their ownership of financial investments over time. Cognition emerges as one of the strongest predictors of the type and number of financial products that individuals hold. Specifically, the results show a positive relation of cognitive level with risky and non-risky financial investments. Even alongside other important factors – such as education, labour status, age, and household wealth – the explanatory power of cognition is found to be significant at the 0.1% significance level. The results are robust when accounting for unobservables and when using a genetic measure of cognition as main explanatory variable. More importantly, cognitive deterioration is only significantly associated with risky financial investments. That means, individuals reduce their risky financial investments when they start suffering old age related cognitive deterioration, while they do not change their holdings in non-risky ones in a significant manner. There are also no significant differences in the reactions to stock-market fluctuations due to cognitive level. In other words, individuals react to stock market fluctuations changing their financial holdings, but those reactions do not differ due to their cognitive level.

Effect of natural aging on mechanical properties and corrosion behavior of 2219 aluminum alloy cold sprayed coatings

The study delves into examining how the natural aging (NA) process influences the mechanical properties and corrosion behavior of cold spray (CS) coatings. The results reveal several key insights: the consolidation behavior of auxiliary steel balls weakens, leading to a transition in the microhardness distribution of the coating from a “stepped” pattern to a more “uniform” pattern, with an average microhardness value fluctuating around 120 HV. Tensile strength experienced a reduction of 4.3 %, and elongation increased by 13.4 %. Electrochemical and peeling corrosion experiments revealed that, after aging, the coating still exhibited no signs of peeling. The corrosion resistance of the coating remains significantly superior to that of the base material (BM). Remarkably, the CS coating continues to provide effective protective functionality for joints even after undergoing 1825 days of NA.

Assessing the impact of anthropogenic influences on the sediment quality of Owalla Reservoir, Southwest, Nigeria

The study aims to investigate the impact of anthropogenic influences within the catchment areas on the sediment physico-chemical quality of Owalla Reservoir in Osun State, Nigeria with the primary aim of creating baseline information on the aspect of limnology. Twenty sampling stations were established along the reservoir representing, its three different sections and two zones. The sampling was conducted for a period of two years to cover both seasons of the year in the area. The sediment samples were collected using a Van-Veen grab of 0.04 m2 area (0.2m × 0.2m) from the waterbed at each sampling locations; labelled, kept in cooler at 4°C and transported to the laboratory for the analysis. The reservoir sediment was mainly clayey-sand in texture, while the particle size distribution was in this order: sand (73.1%) > clay (17.4%) > silt (9.4%). The exchangeable cations order of dominance was in this order: Mg2+ > Ca2+ > H+ > Al3+ > K+ >Na+ and anions in this order: PO34- > SO42- > NO3-> Cl-. The highest mean values for water depth, transparency, air temperature, water temperature and sediment temperature (5.28 ± 0.40 m, 1.60 ± 0.05 m, 31.90 ± 0.29°C, 30.6 ± 0.18°C and 27.6 ± 0.23°C) were recorded during the dry season compared with the rainy season. The results obtained from this study, shows that Owalla Reservoir is fairly clean and not contaminated with toxic pollutants. The sediment pH is within acidic range, and enriched with nutrients due to the anthropogenic activities around this reservoir catchment areas. However, if the organic enrichments and siltation in this reservoir is not controlled, it could lead to the future eutrophication of this waterbody, which can cause water quality degradation, alter the aquatic biota and natural ageing process of this reservoir making it unsuitable for its purposes.

Saponins of Panax japonicus ameliorates cardiac aging phenotype in aging rats by enhancing basal autophagy through AMPK/mTOR/ULK1 pathway

Heart disease is a significant health concern for elderly individuals, with heart aging being the primary cause. Recent studies have shown that autophagy can play a protective role in preventing cardiac aging. Our previous research confirmed that Chikusetsu saponin IVa, a fundamental component of Saponins of Panax japonics (SPJ), can enhance basic autophagy levels in cardiomyocyte of isoproterenol induced cardiac fibrosis mice. However, it remains unclear whether SPJ possesses a protective effect on cardiac dysfunction during the natural aging process. Rats were randomly divided into four groups: adult control group (6 months old), aging group (24 months old), aging group treated with 10 mg/kg SPJ, and aging group treated with 30 mg/kg SPJ. The heart function, blood pressure, and heart mass index (HMI) were measured. Hematoxylin and eosin staining (H&E) and Wheat Germ Agglutinin (WGA) staining were used to observe the changes in morphology, while Masson staining was used to examine collagen deposition in the rat hearts and CD45 immunohistochemistry was conducted to examine the macrophage infiltration in heart tissues. TUNEL kit was used to detect apoptosis level of cardiomyocyte, and western blot was used to evaluate autophagy-related proteins as well as AMPK/mTOR/ULK1 pathway-related markers. SPJ treatment improved the cardiac function, reduced HMI, attenuated myocardial fiber disorder, inhibited inflammatory cell infiltration, and decreased collagen deposition and cardiomyocyte apoptosis in aging rats. Additionally, SPJ treatment decreased the expression of aging-related proteins and restored the expression of autophagy-related markers. SPJ activated autophagy through the activation of AMPK, which in turn increased the phosphorylation of ULK1(Ser555), while inhibited the phosphorylation of mTOR and ULK1(Ser757). Our study demonstrates that SPJ improves the cardiac function of aging rats by enhancing basal autophagy through the AMPK/mTOR/ULK1 pathway. These results offer a theoretical foundation and empirical evidence to support the clinical advancement of SPJ in enhancing age-related cardiac dysfunction.

Effects of Cu and pre-aging on the clustering behavior in Al-Mg-Si alloys

The clustering behaviors in two Al-Mg-Si alloys with and without Cu after different pre-aging and natural aging conditions were systematically quantified using microhardness, differential scanning calorimetry (DSC), and atom probe tomography. Cu addition slowed down the natural aging process after either solutionizing or pre-aging regardless of pre-aging temperature and time, and reduced the negative effect of post pre-aging natural aging on β” precipitation by changing the composition of the Mg and Si rich clusters. This work complements the limited literature on the effect of long natural aging, and provides insights into the impacts of alloy composition and storage time on precipitation behavior during subsequent artificial aging.

Inducing role of thymus in the body’s ageing process

The ageing process should be considered with respect to all homeostatic systems of the body, their importance for the existence of the organism itself and different timing of their switching-on in the process of age-related changes. An attention should be paid to the literature data on age-related changes in the functional activity of immune system, which starts from almost zero at birth, reaches a maximum at the age of about 40-50 years and significantly decreases during the natural ageing process. The vast majority of most socially significant diseases of modern humans are reasonably associated with this last stage of age-related changes in the functional activity of the immune system. So far, there is probably no consensus on whether ageing is a disease, or not a disease, but just a kind of natural wear of the “biological machine”. In any case, one should emphasize that thymic gland is one of the first organs where negative age-related changes are revealed, one of the two central organs of the immune system. Bone marrow is the second central immune organ. Age-related changes in the thymus are not “all or nothing”, “now or never”, they are normally characterized by a gradual decrease of the cell production and changes in their qualitative characteristics, which undoubtedly result into some negative effects upon the indices of immune system activity. There is a need to develop new approaches towards the assessment of functional parameters of the thymus, highlighting the search for therapeutic approaches that would maintain functional activity of the immune system at a high level.

The Comparation of Renal Anti-Senescence Effects and Blood Metabolites between Dapagliflozin and Metformin in Non-Diabetes Environment.

Delaying kidney senescence process will benefit renal physiologic conditions, and prompt the kidney recovering from different pathological states. The renal anti-senescence effects of sodium-glucose cotransporter-2 inhibitors (SGLT2i) and metformin have been proven in diabetic settings, but the roles of each one and combination of two drugs in natural kidney aging process remain undefined and deserve further research. Senescence-accelerated mouse prone 8 (SAMP8) were orally administered dapagliflozin, metformin, and a combination of them for 16 weeks. Dapagliflozin exhibits better effects than metformin in lowering senescence related markers, and the combination therapy shows the best results. In vitro experiments demonstrate the same results that the combination of dapagliflozin and metformin can exert a better anti-senescence effect. Blood metabolites detection in vivo shows dapagliflozin mainly leads to the change of blood metabolites enriched in choline metabolism, and metformin tends to induce change of blood metabolites enriched in purine metabolism. In conclusion, the results suggest dapagliflozin may have a better renal anti-senescence effect than metformin in non-diabetes environment, and the combination of the two drugs can strengthen the effect. The two drugs can lead to different blood metabolites alteration, which may lead to different systemic effects.

Medication-Related Problems and Their Intervention in the Geriatric Population: A Review of the Literature.

In order to implement the principles of providing clinically and economicallyeffective care, the current state of healthcare must be evaluated, and challenges must be addressed. As part of a physician's role in such a context, one tool consists of identifying medication-related problems (MRPs) and accordingly implementing best practices and innovative strategies to improve patient healthcare outcomes. The geriatric population is expected to have passed through the natural ageing process and experienced several physiological and biological changes that impact their bodies and lives.In the presence of geriatric syndromes and the increased number of medications consumed, the risk of MRPs such as polypharmacy, potentially inappropriate medication (PIM), adverse events, drug-drug interactions, and risk of non-adherence increases. Different interventions that focus on practical and perceptual barriers have been studied, and different tools to define clinically important prescribing problems relating to PIM have been established. The Beers Criteria and STOPP (Screening Tool of Older Persons' Prescriptions)/START (Screening Tool to Alert to Right Treatment) criteria are the most widely used sets of explicit PIM criteria; however, they are still limited in Saudi Arabia. These tools should be considered in clinical settings to improve healthcare outcomes in the geriatric population, and the clinical relevance of enhancing medication should also be explored from the point of view of both the patient and healthcare practitioners.

Efectos del control postural en la tasa de error anti-sacádico en personas mayores

BackgroundThe anti-saccadic (AS) error-rate is used to diagnose neurological disorders. The natural aging process could generate difficulty in carrying out parallel neural processes of conscious motor inhibition and eye movement. Therefore, if balance control is imposed on an elderly person in biped positions during an AS movement, an increase in the AS error-rate is expected. ObjectiveTo study the effects of postural control on the AS error-rate in older people. MethodsAn intra and intergroup comparison was made of AS error-rate in an experimental group of older people (PM) and another control group of young people (PJ). For this, blocks of AS and pro-saccadic movements (control) were used randomly in four different postures: (1)sitting (SENT), (2)standing normally (NORMAL), (3)feet together (REDUC), and (4)feet in line (TANDEM). ResultsThe PM group, compared to the PJ group, showed a progressive increase in the AS error-rate from the sitting position to all standing positions, with the maximum AS error-rate in more complex vertical postures. In contrast, the PJ group did not present significative variability of this AS error-rate in all positions. ConclusionsIt is confirmed that the aging process is associated with an increase in the AS error-rate. This study reveals for the first time a significant increase in the AS error-rate when control of body balance is required for PM, implying a decrease in the multiple processing capacity in PM for the execution of complex and parallel tasks.

Life cycle costs and asset management for protective structures against natural hazards

AbstractWith increasing risks from natural hazards in alpine regions in Austria OEBB‐Infra and Hoffmann‐Consult have developed a consistent methodology for condition assessment, prediction, and life cycle optimization of protective structures. Based on a thorough assessment of the asset stock standardized life cycles for key structures like rockfall and avalanche protection structures have been derived. The developed life cycle models allow a generalized assessment of asset condition, residual service life, and remaining asset value. In contrast to typical transport assets, protective structures are both subject to natural aging processes and random events with a certain occurrence rate. Beyond standardized life cycles on network level an adjustment to project level is necessary to account for actual condition development and asset specific events. The paper provides an overview on the developed generic life cycle with application to rockfall protection structures with prices 2022 in Austria.

Ageing effect on ignition sensitivity of lignocellulosic dust

As the use of biomass as a carbon-neutral energy source increases, so does the need for storage facilities, especially for those that are highly seasonal. Consequently, the stored materials may be subject to a natural ageing process before they are used. Such ageing can modify the deflagration parameters of the materials since it can reduce hygroscopicity, increase stiffness and brittleness and change chemical composition in terms of cellulose, hemicellulose and lignin contents. Hydrothermal treatment is a process that accelerates the ageing of wood and lignocellulosic materials. In this work, several lignocellulosic powders from industrial processes were subjected to accelerated ageing to investigate their influence on flammability properties. Grape marc, cork flour, olive pomace, wood dust and lignocellulosic residues from processing waste were selected based on their morphology, chemical characterization and lignin/cellulose content. Based on literature data, ageing conditions in terms of temperature and humidity were chosen to reproduce naturally aged materials. In this study, the effects of ageing on the minimum ignition energy (MIE) and combustion/pyrolysis behaviour were investigated by chemico-physical screening and thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) in an inert and oxidative atmosphere. Results showed how ageing can change the risk of ignition. For example, the MIE of grape marc decreases, while wood-based samples do not ignite after ageing. The decrease in the case of grape pomace is consistent with the decrease in lignin content and moisture. At the same time, passivation of the particle surface could explain the behaviour of the wood samples.

Time- and Gender-Dependent Alterations in Mice during the Aging Process.

Compared to young people and adults, there are differences in the ability of elderly people to resist diseases or injuries, with some noticeable features being gender-dependent. However, gender differences in age-related viscera alterations are not clear. To evaluate a potential possibility of gender differences during the natural aging process, we used three age groups to investigate the impact on spleens, kidneys, and adrenal glands. The immunofluorescence results showed that male-specific p21 proteins were concentrated in the renal tubule epithelial cells of the kidney. Histological staining revealed an increase in the frequencies of fat vacuoles located in the renal tubule epithelial cells of the cortex, under the renal capsule in the kidneys of male mice with age. In female mice, we found that the width of the globular zone in the adrenal gland cortex was unchanged with age. On the contrary, the male displayed a reduction in width. Compared to females, the content of epinephrine in adrenal gland tissue according to ELISA analysis was higher in adults, and a greater decline was observed in aged males particularly. These data confirmed the age-dependent differences between female and male mice; therefore, gender should be considered one of the major factors for personalized treatment in clinical diagnosis and treatment.