National Agricultural Research Center Research Articles

Anti-stress phytohormones impact on proteome profile of green gram (Vigna radiata) under salt toxicity

Anti-stress phytohormones impact on proteome profile of green gram (Vigna radiata) under salt toxicity

EFFECT OF INDIGENOUS VARIETY OF ALOE VERA ON BACTERIOCIN PRODUCTION BY PROBIOTICS CULTURE

Background: Aloe vera has long been used as a medicinal plant in the preparation of various gels, lotions and ointments besides its use in various food products.Methodology: The present study was carried out at the Plants Genetics Research Institute, National Agricultural Research Centre, Islamabad. For the purpose of this study, an indigenous variety of Aloe vera was taken along with probiotics culture of Lactobacillus acidophilus, Lactococcus lactis and Lactobacillus helveticus. Aloe vera gel matrix was separated from outer cortex of leaves and media were prepared for the growth of respective probiotics cultures. Aloe vera was used in each probiotic culture against two pathogenic strains, i.e E. coli and S. aureus. In one plate, tryptone was replaced by 1% Aloe vera extract, while 0.5% and 1% Aloe vera extracts were added to other two plates. All the three probiotic cultures were separately inoculated in test tubes, which were incubated at 370C for 24 hours.Results: After incubation of 24 hours, plates were observed on the next day for the measurement of inhibition zones. Strong to medium zones of inhibition were formed against E. coli and S. aureus for all three probiotic cultures when 1% Aloe vera extract was used.Conclusions: This study revealed that the indigenous variety of Aloe vera exhibited significant anti microbial properties.

私達の線虫研究の歩み－IPM 研究と防除手段30年の転変－

私達の線虫研究の歩み－IPM 研究と防除手段30年の転変－

Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan)

A total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements.

Evaluation of sperm mobility activation media for egg Fertiliztion in locally bred American channel catfish (Ictalurus punctatus)

Fish sperm motility is now thought to be the prime indicator for the peculiarity of fish spermatozoa. Sperm motion characteristics from over 305 different fish species have been published in over 1450 scholarly articles spanning a wide variety of issues, from molecular biology to ecology. The purpose of the current study was to evaluate the impact of two activation media (A, B) created in the lab and distilled water as a control on male American channel catfish milt quality and fertilization rate (Ictalurus punctatus). Brooders were developed from locally bred catfish seed and selected on the basis of maturity at Aquaculture and Fisheries Program, National Agriculture Research Center (NARC), Islamabad Pakistan. The experiment used a complete randomized design (CRD) with three replicates for each of the three treatments: media A, B, and Control (distilled water). As considerable number of sperms are present in every microliter of sperm, hence several forms of activation or dilution media were utilized to utilize sperm or milt more effectively. Following milt collection, it was diluted in a ratio of 1:29 with the aforementioned matching activation media before being subjected to in vitro testing for a number of parameters, including sperm mobility, sperm mobility duration, and sperm viability. The same diluted milt aliquots were used to check fertilization rates, and the findings showed that the components were substantially different in the specified conducts (p

The effect of seed density, variety and soil inoculant on the yield of soybean

The research was established in one growing season (2018), in 4 repeats, on 10 square meter random layout plots in the research farm of the Department of Field Crops Research of National Agricultural Research and Innovation Centre, in Szeged-Öthalom.
 In our investigation we used two different seeding rates (40 and 60 seeds m-2) on two soybean varieties (Pannónia kincse, Bahia). This study was carried out in inoculated field soil and non-inoculated field soil. We determined the yield and evaluated our results with ANOVA according to the different seeding density and varieties.
 From the results of our one-year survey, we could determine that Bahia had a higher yield than Pannonia kincse, which could be proved at 5% level of significance. Based on this data, we can see that the 60 seeds m-2 plots produced higher yields than the lower seed density (40 seeds m-2) plots. The results show that soybean yields increased as a result of soil inoculation.

Impact of nutrient level and seed density on the yield of some winter wheat varieties

The effects of four different nutrient levels (60 kg ha-1 N, 0 kg ha-1 P2O5, 0 kg ha-1 K2O; 90 kg ha-1 N, 30 kg ha-1 P2O5, 30 kg ha-1 K2O; 120 kg ha-1 N, 60 kg ha-1 P2O5, 60 kg ha-1 K2O; 150 kg ha-1 N, 60 kg ha-1 P2O5, 60 kg ha-1 K2O), as well as three different seeding rates (300, 500 and 700 seeds m-2) on different winter wheat breeds have been investigated in this publication. The research was established in one growing season (2017/2018), with 5 winter wheat varieties (GK Arató, GK Bagó, Cellule, Lithium, GK Petur), in 4 repeats, on 10 square meter random layout plots in the research farm of the Department of Field Crops Research of National Agricultural Research and Innovation Centre, in Szeged-Öthalom. We determined the yield and evaluated our results with analysis of variance according to the different nutrient levels and seeding rates. Increasing the seed density, the yield of winter wheat increased too, in a decreasing rate. Increased nutrient inputs resulted in higher yields. The reaction to the fertilizers was very different among the varieties, we could show different yield order at different nutrient levels and seed density. Our results proved that the use of different varieties and agrotechnical elements cause a big difference in yield, which also determines the economic efficiency of the farm.

Survey of root knot nematodes and RMi resistance to Meloidogyne incognita in soybean from Khyber Pakhtunkhwa, Pakistan

The root knot nematode (RKN), Meloidogyne spp. is one of the most significant pests limiting the agricultural productivity. The RKN constitute the most widely distributed group among the plant parasitic nematodes. This study was carried out to find the pathogenicity of Meloidogyne incognita in soybean. Occurrence and prevalence of Meloidogyne species in soybean fields along with categorization of soybean varieties on the basis of resistance to M. incognita was also studied. An extensive survey for RKN was carried out from Khyber Pakhtunkhwa (KPK) province of Pakistan. The most prevalent specie in all studied hot spots was M. incognita, followed by M. javanica that was observed in Akbar Pura, Bari Kot, and Dargai. In this study, fifteen soybean varieties were generously provided by agricultural field’s farmers and National Agriculture Research Centre of Pakistan. These selected varieties were assessed against M. incognita in experimental area of National Nematological Research Center, University of Karachi in three separate trials from 2014 to 2016. Internationally known susceptible (Bossier and Lee) and resistant (Gregg and Forrest) soybean varieties were checked as positive controls. The reproduction factor (RF) and damage index (DI) were employed to sort out the varieties as resistant or susceptible. Statistically significant differences (P < 0.01) were noticed amongst the studied varieties for RKN reproduction factors such as egg mass index (EMI), gall index (GI) and Juveniles/250 g soil. Moreover, thirty SSR markers were used for the identification of RMi gene, amongst them only three diagnostic markers exhibited the presence of RMi gene in resistant varieties. The resistant genotypes are potential materials against M. incognita. In the present investigation, molecular results confirmed locus of RMi gene in resistant genotypes and their functional presence in nematode management programs for choosing resistant varieties in the field. For the confirmation of resistance in soybean against RKN different biochemical indicators, which play active role in resistance mechanism, were also studied. Resistant varieties exhibited maximum amount of phenol, salicylic acid, chlorogenic acid and ascorbic acid as compared to susceptible plants.

Salt tolerance screening of seven rice landraces

Rice is grown on about one-tenth of the earth’s arable land. It is the single largest source of food energy to more than half of the world population. Salinity confines the efficiency of production at the mature stage. Soil salinity decreases the plant growth rate and can harshly limit the crop production. Salinity tolerance differs extensively between species of plants and within species A pot study was conducted to evaluate the salt tolerance of rice varieties under different salinity cum sodicity levels [S0=2.70dSm-1+ 11.38 (mmol L-1)1/2, S1= 5.16dSm-1 +27.88 (mmol L-1)1/2, S2= 6.15 dSm-1 +30.38 (mmol L-1)1/2 and S3= 6.78 dSm-1 +33.74 (mmol L-1)1/2 ]. Seeds of seven rice varieties namely PB-95, SRI-12, T-05, No 1121, SRI-13, Basmati-515 and Shaheen Basmati were used for screening against salt tolerance in pots with different salinity cum sodicity combinations at green house of Land Resources Research Institute, National Agricultural Research Centre, Islamabad, Pakistan during, 2017. Completely randomized deign was applied with three repeats. Data on grain yield were collected. This study showed significant differences in grain yield with seven rice varieties. At S0 [2.40dSm-1+ 11.38 (mmol L-1)1/2] Basmati-515 rice variety gained the top position in grain yield (4.18 tha-1) and the lowest position was attained by T-05 with grain yield (3.41 tha-1). PB-95 produced the highest paddy yield (3.18tha-1) and Shaheen Basmati attained grain yield (3.06tha-1). All other rice under S1 [5.106dSm-1+27.88 (mmol L-1)1/2] showed the lowest grain yield. Under S2 [6.15dSm-1 +30.38 (mmol L-1)1/2] PB-95 rice variety attained the highest grain yield (2.16 tha-1). Rice line 1121 also received the least position (1.66tha-1) in this salinity level. % decrease at S2 over S0 was indicated salt tolerance of rice varieties. PB-95 rice variety attained the lowest % decrease at S2 over S0 (45.17). PB-95 rice variety attained the highest grain yield (1.08tha-1) at S3 [6.78dSm-1 +33.74 (mmol L-1)1/2]. Rice line 1121 also received the least position (0.62tha-1) in this salinity level. % decrease at S3 over S0 was indicated salt tolerance of rice varieties. PB-95 rice variety attained the lowest % decrease at S3 over S0 (72.58). Therefore, this variety had the maximum salt tolerance than other rice varieties under this experiment.

Effect of the Planting Location (Elevation) on the Composition of Fatty Acids in Olive Oil

In Jordan there are different environments and altitudes that have an effect on olive oil properties and fatty acid composition, which therefore affect its quality. Taking into account that there are no previous similar studies in this field, this study investigates the ratios and composition of the main fatty acids in mixed olive oil from different areas in Jordan (above and below the sea level); namely the Jordan Valley, Subaihi, Kufranja and Madaba areas where the elevation ranges between (-230 m) below sea level and (+785 m) above sea level. The study measures the impact of the location of olive orchards on the fatty acid composition of olive oil and of its quality. Three olive oil samples were collected from the &amp;ldquo;Nabali Muhasan&amp;rdquo; and &amp;ldquo;Baladi&amp;rdquo; varieties from each area. They were analyzed in the National Agriculture Research Center (NARC) laboratories using GAS Chromatography. The results showed that the ratios of fatty acids are significantly affected by the degree of above elevation of the olive trees sea level. This confirms the impact of the geographical and climatic environment, which is reflected on the olive oil quality due to differences in the ratios of fatty acids in the four planting areas. This shows that the mean concentration of the fatty acids in the olive oil in these four planting areas is not equal. In general, there is a significant difference in the fatty acid concentrations compared to the standard concentration because of the planting location of olive trees, given that olive oil fatty acids in each location work together in a balanced integrative relationship.

Assessment of genetic diversity in round gourd (Praecitrullus fistulosus) germplasm of Pakistan considering morphological characters

Round gourd (Praecitrullus fistulosus (Stocks) Pangalo), generally familiar as Indian round gourd, apple gourd and Indian baby pumpkin, is a squash type cucurbit, cultivated for its immature fruit, a vegetable notably trendy in South Asia. This rare squash type gourd is native to India. For carrying out this research, 25 round gourd accessions were obtained from National Agricultural Research Centre (NARC), Islamabad, Pakistan, for estimation of their morphological variations on the basis of different vegetative and reproductive attributes. Multivariate analysis of variance (Principal component analysis and Cluster analysis) was performed for grouping round gourd accessions on the basis of similarities in morphological parameters of leaves, vine, flowers and fruits using XLSTAT (2017). One way analysis of variance (ANOVA) was practiced for statistical analysis and significant variations between means were recorded by Duncan’s multiple range (DMR) test at P < 5% for quantitative data of morphological characters. Morphological variation was most apparent in leaf size, leaf color, fruit shape and fruit skin color. Accession 20399 was morphologically very close to other accessions except for its cordate leaf shape whereas rest of the accessions had lobed leaves. Results of the principle component analysis for quantitative morphological traits indicated that the first two PCs explained 32.21% and 14.91% (a total of 47.12%) of the total variation. Cluster analysis grouped all round gourd accessions of Pakistan into two main clusters with 61.65% variability within class and 38.35% diversity between classes. The work presented here is the first report about morphological characterization of Pakistani P. fistulosus germplasm.

The Impact of Knowledge Documentation Process as an Intermediary Variable among Knowledge Acquisition Process, Organizational Culture and Human Capital

The aim of this paper is to investigate the impact knowledge acquisition process, knowledge documentation process, on human capital, and impact organizational culture on documentation process, Accordingly, a questionnaire-based survey was designed to test the aforementioned model based on dataset of 302 employees&amp;rsquo; from the National Agriculture Research Center (NARC) in Jordan, questionnaires which include 29 items were used to gather information from the respondents. Multiple regression and simple regression analyses were conducted to test the research hypotheses. This study identified knowledge acquisition and knowledge documentation are the most important factors affecting the accumulation of human capital. The results indicated that knowledge acquisition process and knowledge documentation process positively and significantly affect human capital. However, organizational culture did not prove to be positively related to knowledge documentation process. Moreover, knowledge documentation process positively and significantly mediated the relationship between knowledge acquisition process and human capital. The results have enormous implication for the government sector in Jordan.

Effect of salinity, humic acid, biozote and vermicompost on soil physicochemical properties and olive plants species

The study was carried out at National Agricultural Research Centre (NARC) Islamabad during August, 2017 to October, 2017 to evaluate the impact of salinity plus humic acid, Biozote and Vermicompost on 3 olive varieties (Nocellera, Frontoio and Carolea) on physicochemical properties of soil and plant ionic concentration used in plastic nursery bags of three months olive sprouted cuttings in tunnel under saline conditions. The completely randomized design was applied with three replications. Olive soil salinity was developed artificially with the mixture of different salts at 2.0 dSm-1. Biozote, humic acid, and Vermicompost were applied in the artificially developed soil salinity filled in polythene bags planted three months olive cuttings. Treatments were; humic acid solid mixed with soil at the time of planting, humic acid liquid to the soil at the time of planting, humic acid sprayed to cuttings (after every 10 days), dip cuttings in humic acid at the time of planting. Addition of vermizote in soil at the time of planting, addition of Biozote in soil at the time of planting and dip cutting in biozote at the time of planting. Results showed that salinity negatively affected the uptake of nutrient elements. Physiochemical properties of soil showed significant variations among different treatments when interacted with varieties at P<0.05. This study pointed out the tolerance and sensitivity levels against salinity in three olive varieties at 2.0 dSm-1. Nocellera olive variety is the most tolerant variety in terms of Na uptake as compared to the others. Whereas the Frontoio olive cultivar is the most susceptible olive variety against salinity stresses. As K uptake is concerned, Nocellera is the variety of maximum uptake and Carloea olive variety with the minimum. The results depicted that Nocellera olive variety proved to be the most salinity tolerant variety.

Calculation nitrogen and sodium budget from lysimeter-grown short-rotation willow coppice experiment

Alternative water resources utilization should take priority over the conventional irrigation water resources (surface and subsurface waters) in the future in Hungary as well, similarly to the global trends because of the climate change impacts. However because of the environmental risks (e.g. nitrate leaching and soil salinization) the reuse of the wastewater require sustainable practices hence the farmers and researchers are responsible for development soil management practices and irrigation principles. Aim of our study was to determine the impact of a wastewater originated from an African intensive catfish farm on the nitrogen budget of the soil-water-plant system in order to evaluate the nitrogen substitution effect and the risk of the nitrate leaching. On the other hand, the aim of the study was to calculate also sodium budget to assess the risk of the sodicity regard to the high sodium concentration of the wastewater. The experiment was conducted at the National Agricultural Research and Innovation Centre (NAIK), Research Institute of Irrigation and Water Management (OVKI) in Szarvas, Hungary. The experiment was set up in the NAIK ÖVKI Lysimeter Station in 2014 with energy willow. During the study (between 03.07.2015 and 21.04.2017) irrigation water quality, rainwater quality, willow N and Na uptake by stems and N and Na leaching was measured. Nitrogen and sodium budget were calculated for two years (2015, 2016) from these data. According to our results the wastewater had high nitrogen content what was able to increase the nitrogen amount in the examined budget however without supplementary fertilizer it could not able to balance the budget only just at W60 treatment (irrigation with wastewater: N concentration 22,7 mg/l). The wastewater had high environmental risk as soil sodicity according to results of the sodium budget.

In-vitro Assessment of Heavy Metal Removal from Contaminated Agricultural Soil by Native Plant Species

Soil pollution by toxic metals such as cadmium (Cd), lead (Pb), chromium (Cr) and copper (Cu) is major problem in different agricultural areas of Pakistan especially Gujranwala and Lahore. The objective of this study was to assess the accumulation and uptake of toxic metals from contaminated soil by using local plants such as Soybean (Glycine max), Barley (Hordeum vulgare), Sunflower (Helianthus annuus), Pearl millet or Pearl millet (Pennisetum americanum), Maize (Zea mays), Canola (Brassica napus) and Wheat (Triticum aestivum). This study was based on the comparison of heavy metal concentrations and their accumulation in different parts of plants. In-vitro experiments were conducted by collecting soil from peri-urban areas of Gujranwala and Lahore being irrigated with untreated wastewater. Different seed varieties of local plants were brought from sale shop at National Agriculture Research Center (NARC) Islamabad. Seeds were germinated and grown in laboratory in ambient environment. Plants were irrigated with different levels of each toxic metal and a control was also run. Plant samples were harvested on 20th day and heavy metal concentrations were checked in roots and shoots of plants. Accumulation of heavy metals in the shoots and roots of local plants was measured using Flame Atomic Absorption Spectrophotometer (FAAS). The evidences provided by this experiment indicated that native crops like maize and canola were effective for phytoremediation of toxic metal polluted soil. In the future further experiments should be performed in order to investigate the phytoremediation potential of these crops for metals.

EFFECT OF INOCULATION METHODS OF BIOZOTE-MAX (PLANT GROWTH PROMOTING RHIZOBACTERIA-PGPR) ON GROWTH AND YIELD OF RICE UNDER NATURALLY SALT-AFFECTED SOIL

A field experiment was carried out at the experimental farm of Soil Salinity Research Institute, Pindi Bhattian during 2016-17 to assess evaluate different Rhizobial inoculation methods on growth and yield of rice (Oryza sativa) cv. Basmati-385 under naturally salt-affected soil (pH= 8.12, ECe= 7.88 dS m-1and SAR=24.96). Seeds of rice were inoculated with Biozote–Max (Plant Growth Promoting Rhizobacteria-PGPR) produced in Soil Biology and Biotechnology Research Programme of Land Resources Research Institute, National Agricultural research Centre (NARC), Islamabad under PARC-Agrotech. Company (Pvt) Ltd. NARC, Islamabad in three ways i.e. rice seeds inoculated for direct seeding and nursery and dipping seedling roots in the solutions of these rhizobial strains comparable of control un-inoculated (control) by each inoculation method. The performances of Rhizobial strain Biozote–Max used as seed and seedling root dipping inoculation were superior to uninoculated control in all the parameters of the rice crop. Among the treatments, seed and seedling root dipping inoculation with Rhizobial strain Biozote–Max performed best in recording plant height, panicle length, number of tillers, 1000-grain weight and grain yield of rice crop. Maximum tillering was observed with all strains under different inoculation methods. Although, the strains performed better as compared to control, however, dipping of nursery roots produced significantly higher yield followed by seed inoculation for direct seeding. Overall, among all the inoculation methods seedling root dipping produced comparable paddy yield. The highest paddy yield (367 g m-2) was harvested with SBCC (M8) seed inoculation which was 13% more than that of un-inoculated (control).

Inducing salt tolerance in wheat through inoculation with rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase activity

Wheat (Triticum aestivum) is the fundamental feed of people as it contributes 60% of the daily diet of ordinary man in Pakistan. Salinity is one of the most imperative stresses that hinder agricultural productivity in nearly every part of the world. Improved biosynthesis of ethylene in plants under salinity stress is well established. Higher ethylene concentration retards root growth and eventually disturbs the overall plant growth. Plant growth promoting Rhizobacteria (PGPR) emits 1-aminocyclopropane-1-carboxylate (ACC) deaminase action under salt stressed conditions which minimizes the power of ACC and ethylene justifying the lethal effects of salt stress on plant growth. The seeds inoculated with PGPR having ACC deaminase are comparatively more tolerant to salt stress. The study was conducted at National Agriculture Research Centre Islamabad to examine the influence of PGPR on wheat growth (cultivars Pak-13 NARC-11 and NARC-09) and ionic concentration under saline environment to see the impact of bacterial strains having ACC deaminase on wheat growth and ionic concentration. The experiment was set up following completely randomized design with three repeats. Wheat seeds were inoculated following rhizobacteria strains, WPR-61, WPR-51, WPS-09 and Consortium of WPR-61, WPR-51 and WPS-09. Salinity (10.62dS m-1) was artificially developed using salts. Shoot and root length significantly affected by different rhizobial strains. The maximum root and shoot length attained by the consortium of three strains. The best results were achieved on NARC-09 wheat variety.Pak-13 wheat variety was significant affected by different rhizobial strains and maximum phosphorus concentration attained by WPS-09 strain in Pak-13.

FIRST REPORT OF BACTERIAL HEAD ROT DISEASE CAUSED BYPECTOBACTERIUM ATROSEPTICUM ON SUNFLOWER IN PAKISTAN

is an important oil seed crop in Pakistan. During a field visit at National Agricultural Research Center (NARC), Islamabad, Pakistan bacterial head rot disease caused by Pectobacterium atrosepticum (formly Erwinaia caratovora subsp. atroseptica) has been observed on both local and exotic hybrids of Sunflower crop. Pathogenicity test has been carried out to fulfill Koch’s postulates. White and creamy colony growth was observed upon isolation and purification of the bacteria. Biochemical tests were conducted at Crop Diseases Research Institute (CDRI), NARC, Islamabad. Pathogenicity test has confirmed the presence of Pectobacterium atrosepticum on sunflower. This is first report documented this pathogen on sunflower crop in Pakistan.

ANTAGONISTIC POTENTIAL OF TRICHODERMA HARZIANUM AGAINST FUSARIUM OXYSPORUM F. SP. CUBENSE ASSOCIATED WITH PANAMA WILT OF BANANA

Fusarium oxysporum f. sp. cubense is a soil loving pathogen of banana that can cause distortion of vascular system. It is responsible for the disease Fusarium wilt of bananas also renowned as panama wilt disease that has responsible of immense losses in the banana industry worldwide. In this study, diseased samples were collected from rhizosphere of banana plants in the research area of National Agriculture Research Centre (NARC), Islamabad and cultured on PDA to isolate pathogenic strains of F. oxysporium f. sp. cubense. Antagonistic potential of Trichoderma harzianum against F. oxysporum f. sp. cubense was evaluated under controlled conditions. T. harzianum produced up to 75.5% inhibition of colony growth of the pathogen followed by incubation for 72 h at 28+2°C in vitro. In pot culture T. harzianum considerably reduced disease severity. It proves that it is a potential biological control agent against banana wilt pathogen.

Faunistic studies of the tribe Brachinini (Carabidae: Coleoptera) from northern Pakistan.

A faunistic study of the tribe Brachinini from northern Pakistan was carried out including extensive fieldwork collections and specimens housed in the Insect Repository, Pakistan Museum of Natural History, Islamabad and the National Insect Museum, National Agricultural Research Centre, Islamabad. Ten species belonging to three genera were identified from the study area, including four new records for Pakistan, Brachinus (Brachinus) ejaculans Fischer Von Waldheim, 1828, Brachinus (Neobrachinus) pallidipes Reitter, 1919, Pheropsophus (Stenaptinus) consularis (Schmidt-Goebel, 1846) and Pheropsophus (Stenaptinus) lineifrons Chaudoir, 1850. A key for these species is provided. A new combination is proposed for Brachinus (Brachynolomus) pallidipes Reitter, 1919, namely Brachinus (Neobrachinus) pallidipes Reitter, 1919, comb. nov.