Abstract
A total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements.
Highlights
IntroductionMostly for the production of malt and food
Barley is economically significant, mostly for the production of malt and food
The result of our study concluded that, the accessions of H. vulgare L. exotic gene pools shows great genetic diversity within their agro-morphometric parameters and within total seed storage protein profile assay detected through polyacrylamide gel electrophoresis SDS-PAGE technology
Summary
Mostly for the production of malt and food. The value of this crop has increased due to its consumption as a functional food with a range of beneficial effects on health (Hua et al, 2015). Application of barley such as malt in brewing, animal feed, human consumption and the distilling industry make it a globally important crop. Cultivators choose the best variety of barley according to specific climate and its applications such as the distilling industry, human consumption, animal feeding and malt preparation (Ferreira et al, 2016). There have been many studies involving testing of barley diversity through many parameters in which genetic diversity in both wild and cultivated barley has been reported (Baik & Ullrich, 2008; Yahiaoui et al, 2008; Ledovskoy et al, 2010; Khodayari, 2012; Nandha & Singh, 2014)
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