Narrow Depth Range Research Articles

Seiche‐Induced Fish Kills in the Sea of Galilee—A Possible Explanation for Biblical Miracles?

AbstractIn Lake Kinneret (the biblical Sea of Galilee), Israel, internal waves of significant amplitude are induced by westerly winds. These waves give rise to upwelling into the surface mixed layer of colder, oxygen‐depleted water from the hypolimnetic and metalimnetic layers. If upwelling occurs soon after the onset of annual thermal stratification, when surface mixed layer extends over a narrow depth range, but the hypolimnion is already anoxic, there is a potential for massive fish kills as fish cannot escape the anoxic water that intrudes into the surface mixed layer along the western shore. This study uses a coupled three‐dimensional atmosphere‐lake model to elucidate the mechanisms behind these infrequent major fish kills in Lake Kinneret. Remarkably, nowadays fish‐kill events happen at the same location in the lake where the biblical Miracle of Loaves and Fishes and presumably the Miraculous Catch of Fish occurred two millennia before the present and may explain the appearance of large numbers of easy‐to‐collect fish close to the shore described in the biblical narratives.

Shallow Focal Depths of the 2022 Ms 6.8 Luding Earthquake and Its M ≥3 Aftershocks

Abstract The accurate determination of earthquake focal depths is crucial, yet notably challenging. The 2022 Ms 6.8 Luding earthquake sequence is a typical example for which focal depths remain controversial. In this study, we conducted a comprehensive regional waveform analysis, including depth-phase waveform modeling and relative location methods, to determine the focal depths of the Ms 6.8 mainshock and its M ≥ 3 aftershocks. Specifically, we first inverted the focal mechanisms of these events using the generalized cut-and-paste method. We then determined their absolute focal depths using array-based stacking and Pn depth-phase modeling. For events without clear depth phases, we calculated their relative depths with respect to the known ones using differential travel times of Pg, Sg, and Pn phases. Our results indicate that the mainshock’s hypocenter lies at a depth of 11 km, whereas its centroid appears at a shallower depth of ∼6.5 km. All M ≥ 3 aftershocks are located within a narrow depth range of 3–7 km, in contrast to the deeper focal depths previously obtained using conventional travel-time-based location methods. This shallow seismogenic depth aligns well with the rheological behavior of the crust along the southern Xianshuihe fault, in which the brittle–ductile transition depth is estimated to be about 10km. Our results reveal that the mainshock nucleated at the base of the seismogenic zone and ruptured toward the shallower portion, consistent with the geodetically constrained mainshock slip distribution. Our study also emphasizes the necessity of caution when interpreting focal depths determined by routine travel-time-based methods, especially in regions with sparse seismic station coverage.

Multi-focus Image Fusion via Blind Deconvolution and Registration based on Photoacoustic Microscopy

Abstract Optical resolution photoacoustic microscopy (OR-PAM), which can present the microstructure of biological tissues is useful in the disease diagnosis. However, conventional OR-PAM can only obtain images with high resolution in a narrow range of depths around the focal plane. In this study, we proposed a multi-focus photoacoustic image fusion method based on the blind deconvolution. This method is divided into two steps. First, we used the method of blind deconvolution to obtain an approximate point spread function (PSF) for each photoacoustic images obtained from multiple layers, and then got the corresponding deblurred photoacoustic images. Second, we used the method of multi-focus images registration and fusion to accurately align the multi-focus photoacoustic images and effectively fuse the features of different images. Finally, the proposed method was applied to process the photoacoustic images obtained from photoacoustic imaging experiments on ants with irregular morphology using fast controllable confocal focus PAM (FC-PAM). The results show that our method improves the quality of photoacoustic images, and can quickly obtain multi-focus fused photoacoustic images with high resolution. It indicates that the proposed multi-focus photoacoustic image fusion method based on FC-PAM has great potential in fast imaging of living biological tissues at multiple imaging depths.

Environmental preferences and critical habitat for the velvet belly lanternshark (Etmopterus spinax) in Icelandic waters.

The velvet belly lanternshark (Etmopterus spinax) is a small, bioluminescent shark that is caught as bycatch in many deep-sea fisheries in the Atlantic Ocean. Using data from 10,597 seasonal research survey tows spanning 11 years, the distribution, relative abundance, life history, and environmental preferences of E. spinax in Icelandic waters was examined for the first time. E. spinax (n = 8774) were only captured in relatively deep offshore waters to the south and west of Iceland. Females grew to larger sizes than males and reached 50% sexual maturity at a total length of 50 cm. Females at a late stage of maturity and very small juveniles (<20 cm) were restricted to the central south Icelandic shelf, suggesting that this might be critical habitat for the reproduction of the species. Most of the sharks were captured at depths of 400-500 m, a relatively narrow depth range, and classified as a stenothermic warm-water species with habitat temperature restricted to about 6.3-8.0°C. Teleosts, crustaceans and cephalopods made up most of the diet. There was no indication of a decline in abundance over the time span of the survey. However, climate-induced warming of the deep ocean may shift the distribution of the species to more northerly waters within Iceland.

An experimental study on the effect of chemical additives in coolant on steam explosion

In assessment of severe accident risk in light water reactors (LWRs), steam explosion is a nonnegligible phenomenon following a relocation of core melt (corium) into coolant, and thus various research efforts have been paid to steam explosion. There had been numerous studies showing that the occurrence of steam explosions is influenced by several factors such as melt and coolant temperatures, melt materials, non-condensable gasses, etc. However, most of the existing experiments used deionized (DI) water or tap water as coolant, with little consideration of the effect of chemicals (e.g. boric acid, sodium hydroxide, sodium phosphate) commonly applied in reactor coolant. To examine the effect of the chemical additives in coolant on steam explosion, the present study performs a series of molten Tin droplet-coolant interaction tests using DI water and different chemical solutions, including H3BO3 solutions, NaOH + H3BO3 neutral solutions, and Na3PO4 + H3BO3 neutral solutions. The experimental results show that adding NaOH and Na3PO4 in boric acid solution significantly affects the occurrence probability of spontaneous steam explosion, because of the presence of PO43− and H+ ions. When different solutions have equivalent concentrations of H3BO3, the peak pressure values of the spontaneous steam explosion of Sn droplets are similar among various solutions. Compared with those in DI water, steam explosion in the chemical solutions occurs predominantly within a narrow range of depth from 28 mm to 40 mm and produces a much higher peak pressure. This implies that more energetic steam explosions may occur in the chemical solutions.

Semi-automated template matching and machine-learning based analysis of the August 2020 Castelsaraceno microearthquake sequence (southern Italy)

The accurate characterization of microearthquake sequences allows seismologists to better understand the physical processes involved in earthquake nucleation and rupture propagation and to gain insights on fault geometry at depth. Standard procedures for seismic sequences analysis are based on manual detection and phase-picking, requiring a huge amount of work from expert seismologists, particularly in the case of microseismic events. Here we show how the investigation of a low-magnitude foreshock-mainshock-aftershock sequence, occurred in August 2020 close to Castelsaraceno village (southern Italy), greatly benefited from the application of a semi-automated template matching and machine-learning based workflow. The phase-picking was automatically performed through a deep-learning algorithm on 202 microearthquakes detected between July and October 2020, followed by an automatic multi-step absolute and relative earthquake location procedure. The 72 relocated events of the seismic sequence were clustered in time (7–12 August) and in a narrow range of depths (10–12 km). The Ml 2.1 foreshock doublet and the Ml 2.9 mainshock identified a persistent asperity. The joint analysis of aftershocks distribution, the mainshock focal mechanism, and the geology of the study area suggest the occurrence of the sequence along a NNE-SSW left-lateral, transtensional fault in the brittle portion of the crystalline basement.

Seamount coral reefs are egg case nurseries for deep-sea skates.

Egg case nurseries of the boreal skate (Amblyraja hyperborea) and Richardson's skate (Bathyraja richardsoni) were defined and mapped on a bathyal seascape (~500-1,900 m depths) south of Tasmania, Australia, using 99 towed-camera transects (157 linear km; N= 50,858 images). In total, 738 skate egg cases were observed (present in 240 images, absent in 50,618); among 113 egg cases examined to identify parent species, 70% were A. hyperborea, 10% B. richardsoni and 20% unidentified Bathyraja species. 'Recently laid' egg cases were differentiated from 'aged' by classifying their colour and condition. The great majority (98%) of egg cases were observed in ~1,100-1,400 m depths on seamounts (15 of 36 surveyed), not seamount bases or adjacent continental slope. Egg cases were associated with reefs formed by accumulated skeletal matrix of the stony coral Solenosmilia variabilis, with >90% egg cases (including most of those recently laid) observed on living S. variabilis that characterises a 'coral zone' in ~950-1,350 m depths. Water in the coral zone is warmer (+0.66 to 2.37°C) than at the deep distributional limits of adult A. hyperborea and B. richardsoni (2,000 and 3,000 m, respectively), potentially providing for accelerated embryonic development. Co-occurrence with living coral infers an energetically favourable local-scale hydrodynamic environment for egg cases, particularly on seamount peaks, where increased water flow over egg cases would avert smothering by suspended sediment, and compensate for lower oxygen concentration compared to deeper depths occupied by adult skates. Criteria identifying egg case nurseries are strongly met for A. hyperborea at Seamount Z110 (468 egg cases of varied ages, maximum density of 5.47 m-2 ) and to a lesser extent on five others (Seamounts K1, Z16, Hill U, Z5 and Hill V). An abundance (density) criterion for defining nurseries needs to be flexible because it is a spatially scale-dependent measure that differs between surveys according to the tools and design employed. Off Australia, coral reef egg case nursery habitat is restricted to a narrow depth range in temperate latitudes where it is scarce and impacted by historical bottom trawl fishing in many locations. There has been effective conservation of nursery habitat, however, because four of the six nursery sites identified here and extensive coral reef areas are protected within marine parks. This article is protected by copyright. All rights reserved.

Do upper-plate material properties or fault frictional properties play more important roles in tsunami earthquake characteristics?

Tsunami earthquakes are a type of shallow subduction zone events that rupture slowly (<1.5 km/s) with exceptionally long duration and depleted high frequency radiation, resulting in a large discrepancy of Mw and Ms magnitudes and abnormally large tsunami along coastal areas. Heterogeneous fault frictional properties at shallow depth have been thought to dominate tsunami earthquake generation. Some recent studies propose heterogeneous upper-plate material properties determine rupture behavior of megathrust earthquakes, including characteristics of tsunami earthquakes. In this study, we use a recently developed 3D dynamic earthquake simulator to explore tsunami earthquake generation and systematically examine roles of upper-plate material properties and fault frictional properties in tsunami earthquake characteristics in a physics-based framework. For heterogeneous fault friction, we consider isolated asperities with strongly velocity-weakening properties embedded in a conditionally stable zone with weakly velocity-weakening properties. For heterogeneous upper-plate properties, we consider a generic depth profile of seismic velocity and rigidity constrained from seismic surveys. We design a set of models to explore their effects on tsunami earthquake generation and characteristics. We find that the conditionally stable zone can significantly slow down rupture speeds of earthquakes that nucleate on asperities to be <1.5 km/s over a large depth range (1–20 km), while heterogeneous upper-plate properties can only reduce rupture speeds to be ∼1.5–2.0 km/s over a narrow depth range (1-3 km). Nevertheless, heterogeneous upper-plate properties promote cascading rupture over multiple isolated asperities on the shallow subduction plane, contributing to large tsunami earthquake generation. We also find that fault frictional properties play much more important roles than upper-plate material properties in long normalized duration, high-frequency depletion and low moment-scaled radiated energy in tsunami earthquakes. In addition, the effective normal stress on the subduction plane, which affects fault frictional strength, also influences the characteristics of tsunami earthquakes, including duration, stress drop and moment-scaled radiated energy.

Pure Inertial Waves Radiating From Low‐Frequency Flows Over Large‐Scale Topography

AbstractMooring data collected at a flat‐topped seamount suggest the generation of pure inertial waves (PIWs; waves with a dominant frequency equal to the local inertial frequency f) by low‐frequency flows over large‐scale topography. Energetic PIWs were observed within a narrow depth range (∼100 m) near the seafloor at the edge of the summit. These waves could be associated with low‐frequency flows. A two‐dimensional nonhydrostatic model was used to show that the observed PIWs are most likely internal wave beams generated by low‐frequency flows over the seamount. Two types of PIWs were identified via observation and model. The first is a PIW that can only travel horizontally. The other propagates upward, with super‐inertial intrinsic frequency that is Doppler‐shifted by the flows to f. Nonlinear triadic interactions among waves with the frequencies [0, f, f] may transfer energy from mean flows to PIWs, promoting energy decay of geostrophic flows over large‐scale topography.

Quaternary coral, coralline algal and vermetid assemblages as sea-level indicators: a review

Corals, coralline algae and vermetid gastropods are indirect (marine limiting) relative sea-level (RSL) indicators. The precision in sea-level reconstruction based on fossils of those organisms depends on the probable palaeodepth in which they grew. Constraining such palaeodepth depends, in turn, on the available information about the habitats of their living counterparts. Diverse genera, species and species assemblages of corals, coralline algae and vermetid gastropods have historically been proposed as reliable indicators of narrow, shallow depth ranges. However, the increased information on depth distribution of marine benthos in the last two decades has challenged some early assumptions about depth ranges of taxa considered diagnostic of precise palaeodepths. Here, the authors test the reliability of coral, coralline algal and vermetid assemblages that have been extensively used in RSL reconstructions in the light of data from Ocean Biogeographical Information System (OBIS) and other recently published data. In the Indo-Pacific province, these data support the use of the robust-branching and the shallow, high-energy encrusting coral assemblages with a 0 to 10 m uncertainty. In both cases many component species have unimodal distributions and both median and average water depths are shallower than 10 m. The reliability of these coral assemblages as indicative of shallow water depths is strengthened when corals are encrusted by thick plants of the coralline alga Porolithon gr. onkodes. According to OBIS data, coralline algae of this species group in the Indo-Pacific are restricted to very shallow waters (95% probability of occurrence shallower than 0.2 m and in 99.6% of records shallower than 6 m). However, such a narrow depth range and the overall scarce data on coralline algal species in the OBIS database are questionable due to difficulties of coralline algal species identification with the naked eye. A comprehensive survey of the modern distribution of coralline algae at One Tree Reef (southern Great Barrier Reef) indicates that P. gr. onkodes has a log-normal distribution with median depth of less than 5 m and 95% of occurrence probability of thick crusts (&gt; 0.2 mm) shallower than 8.8 m. Data on modern distribution of vermetids are scarce. In the OBIS database, vermetid species are reported from relatively wide depth ranges. However, relatively high densities (&gt; 10 individuals per m2) on coral and coralline algal surfaces only occur from above mean low tide to some 6 m depth. In the Western Atlantic-Caribbean province Acropora palmata is the most precise RSL marker and no additional components of fossil assemblages improve its palaeodepth information. The confident use of coralgal and vermetid assemblages as RSL indicators relies on the identification of fossil corals and coralline algae at the species or species-group level. The scarcity of available data highlights the need for further studies on distribution of coralline algal species and vermetid in modern coral reefs from a variety of oceans and reef settings.

364 A Dangerous Case of the “Goldilocks Effect”: Experimental Demonstration of Potential Vascular Injury Mechanism with Central Venous Catheter Insertion

364 A Dangerous Case of the “Goldilocks Effect”: Experimental Demonstration of Potential Vascular Injury Mechanism with Central Venous Catheter Insertion

Spatial patterns in the cover and composition of macroalgal assemblages on fringing and nearshore coral reefs

Context Although increases in macroalgal cover on coral reefs are often reported alongside declines in coral, the composition of algal assemblages and their spatial dynamics are not commonly investigated. Aims To quantify changes in macroalgal assemblage composition over two spatial environmental gradients, depth and distance from shore, within a nearshore reef system in Kimbe Bay, Papua New Guinea, where coral cover has declined. Methods Benthic cover was quantified at three depths (reef flat, 10 and 15 m) on the windward reef slopes of six reefs located three distances from shore (fringing reefs, and platform reefs 100–200 m and 0.7−1 km offshore). Key results Macroalgal cover was highest on the reef flat, and assemblage composition varied among depths and distances from shore. Macroalgal cover was not correlated with coral cover except where macroalgal cover was greater than 20%, where a negative correlation occurred. There was no correlation between macroalgal cover and turf algal cover. All three benthic groups were negatively correlated with the combined total cover of sand and gravel. Conclusions These results indicated a fine-scale spatial structure of macroalgal assemblages on coral reefs over a narrow depth range and short distance from shore and highlighted the importance of a solid substratum. Implications It is likely that the ecological interactions between corals and macroalgae vary considerably over narrow spatial gradients.

Mesophotic coral communities escape thermal coral bleaching in French Polynesia.

Climate change and consequent coral bleaching are causing the disappearance of reef-building corals worldwide. While bleaching episodes significantly impact shallow waters, little is known about their impact on mesophotic coral communities. We studied the prevalence of coral bleaching two to three months after a heat stress event, along an extreme depth range from 6 to 90 m in French Polynesia. Bayesian modelling showed a decreasing probability of bleaching of all coral genera over depth, with little to no bleaching observed at lower mesophotic depths (greater than or equal to 60 m). We found that depth-generalist corals benefit more from increasing depth than depth-specialists (corals with a narrow depth range). Our data suggest that the reduced prevalence of bleaching with depth, especially from shallow to upper mesophotic depths (40 m), had a stronger relation with the light-irradiance attenuation than temperature. While acknowledging the geographical and temporal variability of the role of mesophotic reefs as spatial refuges during thermal stress, we ought to understand why coral bleaching reduces with depth. Future studies should consider repeated monitoring and detailed ecophysiological and environmental data. Our study demonstrated how increasing depth may offer a level of protection and that lower mesophotic communities could escape the impacts of a thermal bleaching event.

Machine-learning-based earthquake locations reveal the seismogenesis of the 2020 Mw 5.0 Qiaojia, Yunnan earthquake

SUMMARY A moment magnitude (Mw) 5.0 earthquake hit Qiaojia, Yunnan, China on 18 May 2020. Its hypocentre is only approximately 20 km away from the Baihetan reservoir, the second largest hydropower station in China. The Baihetan Reservoir is located at the junction of multiple fault zones on the eastern boundary of the Sichuan-Yunnan rhombic block, an area with high background seismic activity. The Baihetan Reservoir was planned to be impounded in April 2021 and the Mw 5.0 earthquake occurred during its water-retaining. Thus, it is critical to investigate the seismogenesis of the Qiaojia Mw 5.0 main shock and evaluate the risk of inducing earthquakes near the Baihetan Reservoir after impoundment. In this study, we built a complete and accurate earthquake catalogue to analyse seismicity in the reservoir area before and after the Mw 5.0 Qiaojia earthquake. We adopted a machine learning-based seismic phase picker, PhaseNet, to automatically detect seismic picks from continuous raw seismic data. Seismic phase picks were associated and located using sequential earthquake association and location methods, including REAL, VELEST and hypoDD. We eventually obtained high-precision locations of 1640 earthquakes by the hypoDD. The distribution of earthquake locations indicates that a concealed fault nearly vertical to the surface accommodated the Mw 5.0 Qiaojia main shock. The majority of its aftershocks is located within a narrow depth range of 8–13 km, indicating that the stresses in the hypocentral area were concentrated near the hypocentre of the Mw 5.0 earthquake. Along with focal mechanism solutions, we suggested that the Mw 5.0 Qiaojia earthquake is more likely a tectonic earthquake. However, we cannot exclude the possibility that earthquakes could be induced after the impoundment of Baihetan Reservoir, because the identified concealed fault is located in the middle of many large fault zones and only 20 km away from the Baihetan Reservoir.

Seasonal evolution of deep phytoplankton assemblages in the Black Sea

The seasonal evolution of the species composition and depth of the deep maximum of phytoplankton (DPM) in the Black Sea has been studied. Vertical series of phytoplankton samples were collected at 278 stations in the deep-water basin during a 35-year period from 1985 to 2019. On average, the bulk of the total phytoplankton biomass is in the upper mixed layer (UML) throughout the year. The maximum total phytoplankton biomass is located in a narrow depth range of 10–15 m and irradiance level of 10–20% of the surface photosynthetically available radiation, regardless of the season. DPM is regularly present from May to September. However, the frequency of its occurrence is low (only 40%) compared to the UML maximum profiles (60%). In the DPM, diatoms predominate in biomass in spring, dinoflagellates in late spring, coccolithophores in early summer, dinoflagellates in summer, and nanoflagellates in autumn. Out of 41 main species, only eight form the maximum biomass in the thermocline or sub-thermocline. Among them are dinoflagellates Tripos muelleri and T. fusus, Diplopsalis lenticula, Pronoctiluca pelagica, and Gyrodinium fusus, which also produce biomass maxima in the UML. Сoccolithophore Acanthoica quattrospina and silicoflagellate Octactis speculum reach maximum biomass in winter in the UML, which indicates that these species just survive at depth during the warm seasons. Cyanobacterium, Synechococcus sp., is the only species which develops en masse exclusively in the thermocline in summer. With this exception, all species growing at depth in the Black Sea cannot be classified as a specific obligate deep flora.

Thermoelasticity of Water in Silicate Melts: Implications for Melt Buoyancy in Earth's Mantle

AbstractA comprehensive analysis of experimental data and theoretical simulations on the partial molar volume of water in silicate melt indicates that finite strain theory successfully describes the compression of the H2O component dissolved in silicate melt at high pressures and temperatures. However, because of the high compressibility of the water component, a fourth order equation of state fit is required to accurately simulate experimental results on water's volume in silicate melts at a deep upper mantle, transition zone, and lower mantle pressures. Data from previous shock compression experiments on hydrous minerals in which melting occurs along the Hugoniot are used to provide an experimental constraint on the partial molar volume of water in silicate melt at deep mantle temperatures and pressures. The equation of state of the water component indicates that, depending on elastic averaging technique, the amount of water that could be present in neutrally or negatively buoyant mafic/ultramafic melts above the 410 km seismic discontinuity is upper‐bounded at 5.6 wt%: smaller than previously inferred, and consistent with melt being confined to a narrow depth range above the 410 km discontinuity. If melt is predominantly distributed along grain boundaries in low aspect ratio films, extents of melting as low as 2% could produce observed seismic velocity reductions. The ability of the lowermost mantle to contain negatively buoyant hydrous liquids hinges on the trade‐off between iron content and hydration: at these depths, substantially higher degrees of hydration could be present within partial melts.

Oxidation state and metasomatism of the lithospheric mantle beneath the Rae Craton, Canada: strong gradients reflect craton formation and evolution

We present the first oxidation state measurements for the subcontinental lithospheric mantle (SCLM) beneath the Rae craton, northern Canada, one of the largest components of the Canadian shield. In combination with major and trace element compositions for garnet and clinopyroxene, we assess the relationship between oxidation state and metasomatic overprinting. The sample suite comprises peridotite xenoliths from the central part (Pelly Bay) and the craton margin (Somerset Island) providing insights into lateral and vertical variations in lithospheric character. Our suite contains spinel, garnet-spinel and garnet peridotites, with most samples originating from 100 to 140 km depth. Within this narrow depth range we observe strong chemical gradients, including variations in oxygen fugacity (ƒO2) of over 4 log units. Both Pelly Bay and Somerset Island peridotites reveal a change in metasomatic type with depth. Observed geochemical systematics and textural evidence support the notion that Rae SCLM developed through amalgamation of different local domains, establishing chemical gradients from the start. These gradients were subsequently modified by migrating melts that drove further development of different types of metasomatic overprinting and variable oxidation at a range of length scales. This oxidation already apparent at ~ 100 km depth could have locally destabilised any pre-existing diamond or graphite.

Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture stop. Placing the phase mask at the aperture stop significantly reduces the size of the device, and varying the focal lengths enables a uniform resolution across a wide depth range. The phase mask encodes the 3D fluorescence intensity into a single 2D measurement, and the 3D volume is recovered by solving a sparsity-constrained inverse problem. We provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the field-varying aberrations in miniature objectives. We demonstrate a prototype that is 17 mm tall and weighs 2.5 grams, achieving 2.76 μm lateral, and 15 μm axial resolution across most of the 900 × 700 × 390 μm3 volume at 40 volumes per second. The performance is validated experimentally on resolution targets, dynamic biological samples, and mouse brain tissue. Compared with existing miniature single-shot volume-capture implementations, our system is smaller and lighter and achieves a more than 2× better lateral and axial resolution throughout a 10× larger usable depth range. Our microscope design provides single-shot 3D imaging for applications where a compact platform matters, such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

The moderate size 2019 September Mw 5.8 Silivri earthquake unveils the complexity of the Main Marmara Fault shear zone

SUMMARYThe unbroken section of the North Anatolian Fault beneath the Sea of Marmara is a major source of seismic hazard for the city of İstanbul. The northern and currently the most active branch, the Main Marmara Fault (MMF), is segmented within a shear zone and exhibits both partially creeping and locked behaviour along its 150 km length. In 2019 September, a seismic activity initiated near MMF, off-coast the town of Silivri, generating 14 earthquakes ≥ Mw 3.5 in a week. The Mw 5.8 Silivri earthquake, is the largest in the Marmara Sea since the 1963 Mw 6.3 Çınarcık earthquake. Our analyses reveal that the activity started in a narrow zone (∼100 m) and spread to ∼7 km following an Mw 4.7 foreshock within ∼2 d. The distribution of relocated aftershocks and the focal mechanisms computed from regional waveforms reveal that the Mw 5.8 earthquake did not occur on the MMF, but it ruptured ∼60° north-dipping oblique strike-slip fault with significant thrust component located on the north of the MMF. Finite-fault slip model of the main shock shows 8 km long rupture with directivity toward east, where the ruptured fault merges to the MMF. The narrow depth range of the slip distribution (10–13 km) and the aftershock zone imply that the causative fault is below the deep sedimentary cover of the Marmara Basin. The distribution of aftershocks of the Mw 5.8 event is consistent with Coulomb stress increase. The stress changes along MMF include zones of both stress decrease due to clamping and right-lateral slip, and stress increase due to loading.

Diverse nitrogen cycling pathways across a marine oxygen gradient indicate nitrogen loss coupled to chemoautotrophic activity.

Genetic markers and geochemical assays of microbial nitrogen cycling processes, including autotrophic and heterotrophic denitrification, anammox, ammonia oxidation, and nitrite oxidation, were examined across the oxycline, suboxic, and anoxic zones of the Cariaco Basin, Venezuela. Ammonia and nitrite oxidation genes were expressed through the entire gradient. Transcripts associated with autotrophic and heterotrophic denitrifiers were mostly confined to the suboxic zone and below but were also present in particles in the oxycline. Anammox genes and transcripts were detected over a narrow depth range near the bottom of the suboxic zone and coincided with secondary NO2 - maxima and available NH4 + . Dissolved inorganic nitrogen (DIN) amendment incubations and comparisons between our sampling campaigns suggested that denitrifier activity may be closely coupled with NO3 - availability. Expression of denitrification genes at depths of high rates of chemoautotrophic carbon fixation and phylogenetic analyses of nitrogen cycling genes and transcripts indicated a diverse array of denitrifiers, including chemoautotrophs capable of using NO3 - to oxidize reduced sulfur species. Thus, results suggest that the Cariaco Basin nitrogen cycle is influenced by autotrophic carbon cycling in addition to organic matter oxidation and anammox.