Narrow Mass Research Articles

Anesthesia Preoperative Clinic Evaluation of Obstructive Sleep Apnea Using Nasal Fiberoptic Videoendoscopy: A Pilot Study Comparison with Polysomnography.

BackgroundNasal fiberoptic videoendoscopy is an established technique to assess upper airway pathology in conscious and sedated patients.ObjectivesThe authors conducted a prospective proof-of-concept pilot study to evaluate whether airway narrowing detected using nasal fiberoptic videoendoscopy in the anesthesia preoperative clinic was capable of defining the severity of obstructive sleep apnea (OSA) in patients scheduled for elective surgery.MethodsAfter application of topical local anesthesia (4% lidocaine with phenylephrine), sixteen patients (ASA physical status 2 or 3) underwent nasal fiberoptic videoendoscopy in sitting position. The magnitudes of retropalatal and retrolingual luminal narrowing were assessed as predictors of OSA. Patients also underwent polysomnography and completed STOP-Bang questionnaires. The endoscopist’s clinical impression of OSA severity based on the history and airway examination was quantified.ResultsRetropalatal luminal narrowing and STOP-Bang score ≥ 4 predicted OSA severity as either “none or mild” or “moderate to severe” in 13 (81%) and 9 (56%) of 16 patients who underwent polysomnography, respectively. OSA severity was significantly (Spearman’s rank correlation coefficient) associated with retropalatal airway narrowing (P = 0.0048), STOP-BANG score (P = 0.0072), and body mass index (P = 0.0091), whereas clinical impression and retrolingual pharyngeal narrowing were not (P=0.093 and P = 0.11, respectively).ConclusionsThe current results suggest that nasal fiberoptic videoendoscopy quantification of retropalatal airway narrowing may be a useful tool for assessing the severity of OSA in the anesthesia preoperative clinic. The current findings document a proof-of-concept feasibility of nasal fiberoptic videoendoscopy as a screening tool for OSA in conscious patients during preoperative evaluation that may justify further prospective clinical trials of this technique.

Flux rope breaking and formation of a rotating blowout jet

We analyzed a small flux rope eruption converted into a helical blowout jet in a fan-spine configuration using multi-wavelength observations taken by SDO, which occurred near the limb on 2016 January 9. In our study, first, we estimated the fan-spine magnetic configuration with the potential field calculation and found a sinistral small filament inside it. The filament along with the flux rope erupted upward and interacted with the surrounding fan- spine magnetic configuration, where the flux rope breaks in the middle section. We observed compact brightening, flare ribbons and post-flare loops underneath the erupting filament. The northern section of the flux rope reconnected with the surrounding positive polarity, while the southern section straightened. Next, we observed the untwisting motion of the southern leg, which was transformed into a rotating helical blowout jet. The sign of the helicity of the mini-filament matches the one of the rotating jet. This is consistent with the jet models presented by Adams et al. (2014) and Sterling et al. (2015). We focused on the fine thread structure of the rotating jet and traced three blobs with the speed of 60-120 km/s, while the radial speed of the jet is approx 400 km/s. The untwisting motion of the jet accelerated plasma upward along the collimated outer spine field lines, and it finally evolved into a narrow coronal mass ejection at the height of approx 9 Rsun . On the basis of the detailed analysis, we discussed clear evidence of the scenario of the breaking of the flux rope and the formation of the helical blowout jet in the fan-spine magnetic configuration.

Search for pair production of vector-like quarks in the [formula omitted] channel from proton–proton collisions at [formula omitted

A search is presented for the production of vector-like quark pairs, TT‾ or YY‾, with electric charge of 2/3 (T) or −4/3 (Y), in proton–proton collisions at s=13TeV. The data were collected by the CMS experiment at the LHC in 2016 and correspond to an integrated luminosity of 35.8fb−1. The T and Y quarks are assumed to decay exclusively to a W boson and a b quark. The search is based on events with a single isolated electron or muon, large missing transverse momentum, and at least four jets with large transverse momenta. In the search, a kinematic reconstruction of the final state observables is performed, which would permit a signal to be detected as a narrow mass peak (≈7% resolution). The observed number of events is consistent with the standard model prediction. Assuming strong pair production of the vector-like quarks and a 100% branching fraction to bW, a lower limit of 1295 GeV at 95% confidence level is set on the T and Y quark masses.

INVESTIGATION THE OLIGOMERIZATION PROCESS OF HEXENE-1

In article for oligomerization and alkylation processes, to obtain a useful and highly stable active catalyst, New Catalytic Complex (NCC) synthesized which is based on aluminum metal using C2H4Cl2 and this complex has shown high stability in alkylation, oligomerization processes compared to other aluminum-containing catalysts. It is difficult to regulate the molecular mass distribution of received products during oligomerization, either Intensificate the oligomerization process of olefins that is why this process take place in presence of obtaining of bimetal catalytic complex with a highly stable activity from the modification of the New Catalytic Complex(NCC) with transition metal chloride. Comparatively studied oligomerization of Hexen-1 in present of new bimetallic catalytic complex which was modified by NCC and chromium salt (NCC/CrCl3), effect of temperature, reaction time, concentration of the catalyst and the proportion of catalyst components (Al:Cr) in the process was investigated.It was determined that at the initial time (10 min) conversion hexene-1 and the yield of purposeful oil fraction were low. Increasing time, conversion rate of Hexen-1 increases and the optimal time for the process is considered as 60 minutes. In low concentrations of catalyst (0,1-0,3) oligomerization of hexene-1 is low but in high concentrations(>1%) reaction goes with high rate, at optimal concentration 0,5% mass both the yield of oil fraction and its chemical-physical properties were high.In oligomerization process investigated ratio effect of catalyst components Al: Cr that was used Al: Cr=(4-32):1 mol.It has been revealed that oligomerization of Hexen-1, at low mol ratio of components (4:1), received yield of oligomer fraction decreases while mol ratio increases (16:1, 32:1).Studies determined that bimetallic complex at 8:1 mole has shown the maximal activity in oligomerization process of Hexen-1.The effect of the temperature on the oligomerization process was studied in the range of 30-60ºC, increasing the temperature up to 50 °C has a positive effect on the course of the process and despite providing complete oligomerization of hexene-1 its also providing increasing the amount of oil fractions with high viscosity. According to the researches, the optimal conditions for the oligomerization process of hexene-1 with the participation of the bimetallic catalytic complex: reaction time – 60 min., concentration of catalyst – 0,5 % by wt., mol ration of components Al:Cr = 8:1, temperature – 50 °C. In known optimal condition its possible to obtain oligomers with physical indications, characterized by narrow molecular mass distribution, by new catalytic complex that was modified by chromium salt.Such products have special importance, as low-freezing, high viscosity index qualitative engine oils.Synthesis of bimetallic catalytic complexes, with their presence in oligomerization of olefins and fractions, opens wide range perspectives in synthesis of different high-molecular compounds in industry

Demystifying the compressed top squark region with kinematic variables

The ongoing perplexing scenario with no hints of new physics at the Large Hadron Collider can be elucidated amicably if the exotic particle spectrum in many of the well-motivated theoretical models possesses degenerate mass. We investigate the usefulness of different kinematic variables sensitive to the compressed mass region, and propose a search strategy considering a phenomenological supersymmetric scenario where the top squark undergoes a four-body decay due to its extremely narrow mass difference with the lightest supersymmetric particle. Considering a challenging but relatively clean dileptonic decay channel, we demonstrate that one can effectively restrain the significant background from the top quark, which provides a complementary approach to the present CMS analysis. With the new strategic approach the current limit can be extended to a phase-space region that was not explored before.

Development and validation of an ultra performance liquid chromatography Q-Exactive Orbitrap mass spectrometry for the determination of fipronil and its metabolites in tea and chrysanthemum

A fast, sensitive and reliable method for the determination of fipronil and its metabolites in tea and chrysanthemum was developed using a modified QuEChERS technique and an ultra performance liquid chromatography Q-Exactive Orbitrap mass spectrometry. The mixture of adsorbents containing primary secondary amine (PSA), octadecylsilane (C18) and carbon nanotubes (CNTs), was used as QuEChERS adsorbents. The use of mass resolution at 70000 full width at half maximum (FWHM) and narrow mass windows at 5 ppm achieved high selectivity and repeatability. Satisfactory linearity with correlative coefficient (R2) higher than 0.996 was achieved for all compounds. Recoveries at three levels (2, 10 and 50 μg kg−1) ranged from 86% to 112%, while the intra- and inter-day accuracies were less than 15%. Limits of quantification for fipronil and its metabolites were 2 μg kg−1, which fulfils the requirement of maximum residue limits formulated by European Union and Japan.

Primordial black hole evolution in two-fluid cosmology

Several processes in the early universe might lead to the formation of primordial black holes with different masses. These black holes would interact with the cosmic plasma through accretion and emission processes. Such interactions might have affected the dynamics of the universe and generated a considerable amount of entropy. In this paper we investigate the effects of the presence of primordial black holes on the evolution of the early universe. We adopt a two-fluid cosmological model with radiation and a primordial black hole gas. The latter is modelled with different initial mass functions taking into account the available constraints over the initial primordial black hole abundances. We find that certain populations with narrow initial mass functions are capable to produce significant changes in the scale factor and the entropy.

Rapid fingerprinting of lignin by ambient ionization high resolution mass spectrometry and simplified data mining

Ambient ionization techniques are typically used to analyze samples in their native states with minimal or no sample pretreatment prior to mass spectrometric (MS) analysis. Desorption electrospray ionization (DESI) and direct analysis in real time (DART) were systematically investigated in this work for direct solid analysis of depolymerized lignin samples, with the goal of rapidly fingerprinting these samples, to efficiently characterize the subunits of this renewable energy source. High resolution MS was required for enhanced selectivity in this study due to the inherent structural complexity of lignin. DESI provided results across a broader mass range (up to m/z 700) than DART and also ionized saturated compounds of low oxygen-to-carbon (O/C) ratios and low double bond equivalents (DBE). While DART detected the same core lignin monomeric and dimeric compounds as seen with DESI and electrospray ionization (ESI), results were restricted to a narrower mass range to m/z 500, due to thermal degradation and losses of methoxy groups. In contrast to DESI and ESI, the DART spectra were nearly void of saturated components. On a structural level, the core lignin compounds were visually fingerprinted and ionization method performances critically assessed by employing simplified Kendrick-based data mining approaches. A novel simplified data visualization approach was developed in this work based on modified Kendrick mass defect (KMD) filtering for lignin subunits and plotting the mass defect values against the degree of unsaturation. Direct visualization of monomeric, dimeric and trimeric lignin species was simplified by the KMD separation plots, easily allowing the core lignin compounds to be visually identified and compared. Modified KMD bases, namely methoxy and phenol bases, which represent monomer-specific lignin constituents, were successfully used to classify and group the complex mixture of lignin species. Further separation of methoxy-related lignin species was successfully achieved by employing the more specific phenolic KMD base.

Application of ultra-high-performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry for identification, confirmation and quantitation of illegal adulterated weight-loss drugs in plant dietary supplements.

In this paper, an ultra-high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap high resolution mass spectrometry (UHPLC-LTQ-Orbitrap HRMS) method was developed and validated for identification, confirmation and quantitation of illegal adulterated weight-loss drugs in plant dietary supplements. 13 wt-loss drugs were well separated by the gradient elution of 10mmol/L ammonium acetate - 0.05% formic acid H2O and acetonitrile at a flow rate of 0.2mL/min within 12min. The MS analysis was operated under the positive ion and in full MS/dd-MS2 (data-dependent MS2) mode. The full MS scan with resolution at 60 000 FWHM and narrow mass windows at 5ppm acquired data for identification and quantitation, and dd-MS2 scan with resolution at 15 000 FWHM obtained product ions for confirmation. The method validation showed good linearity with coefficients of determination (r2) higher than 0.9951 for all analytes. Meantime, all the LOD and LLOQ values were in the respective range of 0.3-2 and 1-9ng/g. The accuracy, intra- and inter-day precision were in the ranges of -1.7 to 3.4%, 1.7-5.0% and 1.9-4.4%, respectively. The mean recoveries ranged from 85.4 to 107.1%, while the absolute and relative matrix effect were in the corresponding range of 98.2-108.6% and 2.6-8.7%. Among 120 batches of weight loss plant dietary supplements, sibutramine and fluoxertine or both were positive in 29 samples. In general, LTQ-Orbitrap HRMS technology was a powerful tool for the analysis of illegal ingredients in dietary supplements.

A LC-HRMS After QuEChERS Cleanup Method for the Rapid Determination of Dye Residues in Fish Products

The reliability of a rapid LC-HRMS method was studied in order to find a sensitive and accurate, simple, cheap, and rapid method for screening and quantitative determination of malachite green (MG), leucomalachite green (LMG), brilliant green (BG), crystal violet (CV), and leucocrystal violet (LCV) in fish muscle. All the analytes were extracted using a mixture of acetonitrile and citrate buffer, while the cleanup phase was carried out by Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method. All the data analyzed were acquired using both full MS and dd-MS2 modes. Good specificity, precision (relative standard deviation <11% for each sample tested), recovery (10–60%), decision limit (CCα between 0.55 and 0.62 μg kg−1), detection capabilities (CCβ between 0.65 and 0.78 μg kg−1), and ruggedness were achieved for the reliability of the method. Satisfactory results were obtained during the linearity test in the range of 0.10–2 ng mL−1 (r 2 > 0.999). Best recoveries were obtained by the QuEChERS cleanup method for all the analytes examined, presenting values between 70 and 104%. The application of 70,000 FWHM mass resolution and narrow mass windows significantly improved the selectivity of the method, leading to simultaneous screening and quantification of dye residues in comparison to other methods proposed in literature. The optimized method proposed in this work enables a simple and fast preparation; it offers exceptional sensitivity and selectivity and maximizes efficiency and reproducibility with a low consumption of reagents. Finally, the present method was successfully employed to detect dye residues in 73 fish samples, as provided for the national residue control plan.

The VIMOS Public Extragalactic Redshift Survey (VIPERS)

We use the full VIPERS redshift survey in combination with SDSS-DR7 to explore the relationships between star-formation history (using d4000), stellar mass and galaxy structure, and how these relationships have evolved since z~1. We trace the extents and evolutions of both the blue cloud and red sequence, by fitting double Gaussians to the d4000 distribution of galaxies in narrow stellar mass bins, for four redshift intervals over 0<z<1. This reveals downsizing in star formation, as the high-mass limit of the blue cloud retreats steadily with time from M*~10^11.2 M_sun at z~0.9 to M*~10^10.7 M_sun by the present day. The number density of massive blue-cloud galaxies (M*>10^11 M_sun, d4000<1.55) drops sharply by a factor five between z~0.8 and z~0.5. These galaxies are becoming quiescent at a rate that largely matches the increase in the numbers of massive passive galaxies seen over this period. We examine the size-mass relation of blue cloud galaxies, finding that its high-mass boundary runs along lines of constant M*/r_e or equivalently inferred velocity dispersion. Larger galaxies can continue to form stars to higher stellar masses than smaller galaxies. As blue cloud galaxies approach this high-mass limit, they start to be quenched, their d4000 values increasing to push them towards the green valley. In parallel, their structures change, showing higher Sersic indices and central stellar mass densities. For these galaxies, bulge growth is necessary for them to reach the high-mass limit of the blue cloud and be quenched by internal mechanisms. The blue cloud galaxies that are being quenched at z~0.8 lie along the same size-mass relation as present day quiescent galaxies, and seem the likely progenitors of today's S0s.

On the gravitational wave background from black hole binaries after the first LIGO detections

The detection of gravitational waves from the merger of binary black holes by the LIGO Collaboration has opened a new window to astrophysics. With the sensitivities of ground based detectors in the coming years, we will principally detect local binary black hole mergers. The integrated merger rate can instead be probed by the gravitational-wave background, the incoherent superposition of the released energy in gravitational waves during binary-black-hole coalescence. Through that, the properties of the binary black holes can be studied. In this work we show that by measuring the energy density ΩGW (in units of the cosmic critical density) of the gravitational-wave background, we can search for the rare ∼ 100 M⊙ massive black holes formed in the Universe. In addition, we can answer how often the least massive BHs of mass ≳ 3M⊙ form. Finally, if there are multiple channels for the formation of binary black holes and if any of them predicts a narrow mass range for the black holes, then the total ΩGW spectrum may have features that with the future Einstein Telescope can be detected.

Products of hydrothermal treatment of lignin and the importance of ortho-directed repolymerization reactions

Alkaline hydrothermal treatment (HTT) of Protobind 1000 lignin was studied to improve the understanding of lignin conversion paths. HTT was performed at 300°C using NaOH as base catalyst. Product separation by acidification and solvent fractionation resulted in a liquid product of low molecular mass and narrow molecular mass distribution and two high molecular mass solid fractions. Twenty volatile compounds accounted for about 36wt% of the liquid product, however, the analysis by liquid injection GC–MS was estimated to detect only about 12wt% of the total products. Analytical pyrolysis was applied to investigate the pyrolyzable fraction of the liquid product and the high molecular mass fractions. The pyrolyzable mass accounted for 49wt% of the total products. The liquid product was quantitatively pyrolyzed at 500°C (91±10wt%) while less than half of the high molecular mass fractions were pyrolyzed. Several chemical features of the pyrolysates suggested that ortho-directed alkylation and condensation reactions of phenolate ions were important for the production of oligomeric and polymeric aromatic compounds during HTT. Increased abundancy of hydroxyphenyl and o-alkyl aromatic pyrolysates in the high molecular mass fractions suggested that the syringyl units of the raw lignin were less affected by repolymerization reactions than hydroxyphenyl and guaiacyl units. These results suggest that the monolignol composition of lignin is an important factor for the valorization of lignin by alkaline hydrothermal treatment.

P‐140: Lamination Technology for Flexible AMOLED

The development of next generation display is toward to the form of novel curved display or flexible display to final users. Due to the characteristic of flexibility, the active matrix organic light‐emitted diode (AMOLED) is suitable to manufacture of these types of display. By laminating process, the AMOLED can be bonded onto the curvature glass to form a curved display or bonded onto the flexible substrate to form a flexible display. In this article, the technical trend and the development of lamination technology are demonstrated. Two types of laminator are showed, the roller type and air bag type, in which the former possess higher accuracy and lower tact time than air bag type. To meet the requirement of manufacture narrow border display and mass production, the equipment with high accuracy and low tact time are necessary. We also showed the method to laminate a curved display and showed the solution to overcome the problem beyond lamination.

Methodology and Characterization of Isolation and Preconcentration in a Gas-Filled Digital Linear Ion Guide.

Digital operation of linear ion guides allows them to operate as traps and mass filters by modulating the duty cycles of the two driving waveforms. A gas-filled (5 mTorr) digitally driven quadrupole ion guide was used to demonstrate ion isolation and preconcentration. These abilities allow ion trapping mass spectrometers to be filled to capacity with only ions in the range of interest at essentially any value of m/z. Due to the unique performance characteristics of digitally operated quadrupoles, isolation with purely duty cycle enhanced waveforms was developed with three increasingly sophisticated isolation methods. First, the guide was used as a gas-filled transmission mass filter using the waveform duty cycle to generate a narrow mass window. The second method used broadband trapping to collect ions and translationally cool along the transmission axis before shifting the duty cycle to filter the trapped ions. A subsequent duty cycle change axially ejected the filtered population for measurement. The third method improved resolution by shifting the operating frequency during isolation. The resolving power was optimized with the shift frequency to yield a device limited resolving power of 400 (m/Δm). It is the temporal control of the duration of the isolation process that sets digital waveform based isolation apart from the current technology and that minimizes ion loss even when the mass is very large. Preconcentration by repeated trapping and isolation of an individual charge state was also demonstrated to saturate the ion guide with that charge state. These digital isolation and preconcentration techniques will permit the same isolation resolution (m/Δm) at any value of mass or m/z without significant ion loss as long as the secular frequencies do not significantly overlap while in the trapping mode. It is therefore ideal for the isolation and preconcentration of single charge states of large proteins and complexes.

Global Post-Translational Modification Discovery.

A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications with high confidence in complex unenriched samples. The G-PTM-D strategy represents a major advance to the previously reported G-PTM strategy and provides a powerful new capability to the proteomics research community.

A study of the effect of rotational mixing on massive stars evolution: surface abundances of Galactic O7-8 giant stars

Context. Massive star evolution remains only partly constrained. In particular, the exact role of rotation has been questioned by puzzling properties of OB stars in the Magellanic Clouds. Aims. Our goal is to study the relation between surface chemical composition and rotational velocity, and to test predictions of evolutionary models including rotation. Methods. We have performed a spectroscopic analysis of a sample of fifteen Galactic O7-8 giant stars. This sample is homogeneous in terms of mass, metallicity and evolutionary state. It is made of stars with a wide range of projected rotational velocities. Results. We show that the sample stars are located on the second half of the main sequence, in a relatively narrow mass range (25–40 M⊙). Almost all stars with projected rotational velocities above 100 km s-1 have N/C ratios about ten times the initial value. Below 100 km s-1 a wide range of N/C values is observed. The relation between N/C and surface gravity is well reproduced by various sets of models. Some evolutionary models including rotation are also able to consistently explain slowly rotating, highly enriched stars. This is due to differential rotation which efficiently transports nucleosynthesis products and allows the surface to rotate slower than the core. In addition, angular momentum removal by winds amplifies surface braking on the main sequence. Comparison of the surface composition of O7-8 giant stars with a sample of B stars with initial masses about four times smaller reveal that chemical enrichment scales with initial mass, as expected from theory. Conclusions. Although evolutionary models that include rotation face difficulties in explaining the chemical properties of O- and B-type stars at low metallicity, some of them can consistently account for the properties of main-sequence Galactic O stars in the mass range 25–40 M⊙.

Primeval very low-mass stars and brown dwarfs – II. The most metal-poor substellar object

SDSS J010448.46+153501.8 has previously been classified as an sdM9.5 subdwarf. However, its very blue $J-K$ colour ($-0.15 \pm 0.17$) suggests a much lower metallicity compared to normal sdM9.5 subdwarfs. Here, we re-classify this object as a usdL1.5 subdwarf based on a new optical and near-infrared spectrum obtained with X-shooter on the Very Large Telescope. Spectral fitting with BT-Settl models leads to $T_{\rm eff}$ = 2450 $\pm$ 150 K, [Fe/H] = $-$2.4 $\pm$ 0.2 and log $g$ = 5.5 $\pm$ 0.25. We estimate a mass for SDSS J010448.46+153501.8 of 0.086 $\pm$ 0.0015 M$_{\odot}$ which is just below the hydrogen-burning minimum mass at [Fe/H] = $-$2.4 ($\sim$0.088 M$_{\odot}$) according to evolutionary models. Our analysis thus shows SDSS J0104+15 to be the most metal-poor and highest mass substellar object known to-date. We found that SDSS J010448.46+153501.8 is joined by another five known L subdwarfs (2MASS J05325346+8246465, 2MASS J06164006$-$6407194, SDSS J125637.16$-$022452.2, ULAS J151913.03$-$000030.0 and 2MASS J16262034+3925190) in a 'halo brown dwarf transition zone' in the $T_{\rm eff}-$[Fe/H] plane, which represents a narrow mass range in which unsteady nuclear fusion occurs. This halo brown dwarf transition zone forms a 'substellar subdwarf gap' for mid L to early T types.

Interpreting 750 GeV diphoton excess with R-parity violating supersymmetry

We propose a supersymmetric explanation of the diphoton excess in the Minimal Supersymmetric Standard Model (MSSM) with the leptonic R-parity violation. In our model, sneutrino serves as the 750 GeV resonance and produced through quark–antiquark annihilation. With introducing appropriate trilinear soft parameters, we show that the diphoton branching ratio is significantly enhanced compared with the conventional MSSM. For current dijet and W-pair LHC constraints, we can successfully fit the observed diphoton signal rate in sizeable parameter regions, the resulting parameter space strongly favor the masses of light smuon and stau within the range from 375–500 GeV, which depends on the choice of electroweakino masses and soft trilinear terms. While after taking into account the compatibility of diphoton excess between the 8 TeV and 13 TeV LHC, only the coupling involved with the second generation quarks is survived. In this case, the corresponding parameter space favors a narrow mass range of smuon and stau with [Formula: see text]. Even if the 750 GeV diphoton excesses were not confirmed by the ATLAS and CMS experiments, we point out that our proposal can still be used to explain the current and future tentative diphoton excesses.

Rapid determination of 30 bioactive constituents in XueBiJing injection using ultra high performance liquid chromatography-high resolution hybrid quadrupole-orbitrap mass spectrometry coupled with principal component analysis

Xuebijing injection (XBJ) is a traditional Chinese herbal prescription widely used in the treatment of sepsis. Extensive chemical studies revealed that XBJ injection contains amino acids, phenolic acids, flavonoid glycosides, terpeneglycosides and phthalides. In this study, the applicability of ultra high performance liquid chromatography coupled with high resolution hybrid quadruple-orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) for the simultaneous quantitative analysis of 30 bioactive constituents in XueBiJing injection (XBJ) was investigated. The mass spectrometer was operated in full MS scan mode. The use of 70,000FWHM mass resolution and narrow mass windows (5ppm) could effectively improve the selectivity of the method. Separation was achieved on a Waters ACQUITY UPLC® HSS C18 column (2.1mm×100mm, 1.8μm) with a gradient mobile phase consisting of acetonitrile-water (containing 10mM ammonium acetate) at a flow rate of 0.2mL/min. Satisfactory linearity was achieved within wide linear range and all correlation coefficients (r) of analytes were more than 0.9996. The limits of detection (LODs) were in the range of 0.1180–27.82ng/mL for different analytes. The relative standard deviations (RSDs) of inter- and intra-day precisions were less than 3.0% and the recoveries of the assay were in the range of 98.5%–101.5%. The validated method was successfully applied for simultaneous determination of 30 bioactive compounds in XueBiJing injection from 10 batches samples by UHPLC-Q-Orbitrap MS within 10min. Moreover, the results were evaluated principal component analysis and two compounds might be the most important chemical markers for chemical quality control of XBJ injection. The novel Q-Orbitrap mass spectrometry has been proved to be a very promising and powerful tool for routine screening of bioactive compounds in traditional Chinese medicine injection, ensuring drug safety and public health.