The effect of variant concentrations of multiwalled carbon nanotubes (MWCNTs) on the thermal transport, phase transition temperatures, and mechanical properties of polyisoprene rubber have been studied in the present novel research. MWCNTs were incorporated into the natural rubber (NR) using shear mixing techniques. Microscopy results reveal the uniform dispersion of the nanotubes within the polymer matrix. Thermal conductivity and thermal insulation of the fabricated composites were evaluated according to ASTM standards. Thermal transport through the nanocomposite specimens is restricted by the nanotubes network developed within the polymer matrix. Differential scanning calorimetric study elucidates the reduction of crystallization (T c ) and glass transition (T g ) temperatures, while melting temperature (T m ) enhances with increasing the nanotubes concentration in the rubber matrix. A remarkable enhancement in mechanical properties of MWCNT/NR composites was observed with increasing nanotube to matrix ratio. techniques to fabricate nanocomposites for engineering applications, i.e., automobile industry, sports industry, membrane technology, aerospace industry, energy storage and many more. In order to fabricate an affective composite, aspect ratio of the nanotube should not be diminished. The CNTs/polymer nanocomposites have also shown outstanding thermal stability in nitrogen as well as in oxygen atmospheres. Uniformly dispersed nanotubes develop a network in a polymer matrix that restricts the thermal mobility of polymeric chains in the heat atmosphere (3). Polyisoprene rubber (NR) with superb thermal/mechanical properties, oil/hot air ageing and swelling resistance has extensively used in automobile and oil industries. Selection and designing of a composite for a specific application necessitate its thermal transport/endurance data. Evaluation of thermal properties for a polymer nanocomposite has great importance to allocate relevance area of application. This paper reports the novel investigation of thermal impedance, thermal conductivity, thermal composition, heat flow response, glass transition/crystallization/melting temperatures, and specific enthalpies of NR nanocomposites with five diverse loadings of pristine multiwalled carbon nanotubes (MWCNTs).