Nanoscale Vibration Research Articles

Developing and Investigating a Nanovibration Intervention for the Prevention/Reversal of Bone Loss Following Spinal Cord Injury.

Osteoporosis disrupts the fine-tuned balance between bone formation and resorption, leading to reductions in bone quantity and quality and ultimately increasing fracture risk. Prevention and treatment of osteoporotic fractures is essential for reductions in mortality, morbidity, and the economic burden, particularly considering the aging global population. Extreme bone loss that mimics time-accelerated osteoporosis develops in the paralyzed limbs following complete spinal cord injury (SCI). In vitro nanoscale vibration (1 kHz, 30 or 90 nm amplitude) has been shown to drive differentiation of mesenchymal stem cells toward osteoblast-like phenotypes, enhancing osteogenesis and inhibiting osteoclastogenesis simultaneously. Here, we develop and characterize a wearable device designed to deliver and monitor continuous nanoamplitude vibration to the hindlimb long bones of rats with complete SCI. We investigate whether a clinically feasible dose of nanovibration (two 2 h/day, 5 days/week for 6 weeks) is effective at reversing the established SCI-induced osteoporosis. Laser interferometry and finite element analysis confirmed transmission of nanovibration into the bone, and microcomputed tomography and serum bone formation and resorption markers assessed effectiveness. The intervention did not reverse SCI-induced osteoporosis. However, serum analysis indicated an elevated concentration of the bone formation marker procollagen type 1 N-terminal propeptide (P1NP) in rats receiving 40 nm amplitude nanovibration, suggesting increased synthesis of type 1 collagen, the major organic component of bone. Therefore, enhanced doses of nanovibrational stimulus may yet prove beneficial in attenuating/reversing osteoporosis, particularly in less severe forms of osteoporosis.

Regional differences in cochlear nonlinearity across the basal organ of Corti of gerbil: Regional differences in cochlear nonlinearity

Auditory sensation is based in nanoscale vibration of the sensory tissue of the cochlea, the organ of Corti complex (OCC). Motion within the OCC is now observable due to optical coherence tomography. In a previous study (Cooper et al., 2018), the region that includes the electro-motile outer hair cells (OHC) and Deiters cells (DC) was observed to move with larger amplitude than the basilar membrane (BM) and surrounding regions and was termed the "hotspot." In addition to this quantitative distinction, the hotspot moved qualitatively differently than the BM, in that its motion scaled nonlinearly with stimulus level at all frequencies, evincing sub-BF activity. Sub-BF activity enhances non-BF motion; thus the frequency tuning of the OHC/DC region was reduced relative to the BM. In this work we further explore the motion of the gerbil basal OCC and find that regions that lack significant sub-BF activity include the BM, the medial and lateral OCC, and the reticular lamina (RL) region. The observation that the RL region does not move actively sub-BF (already observed in Cho and Puria 2022), suggests that hair cell stereocilia are not exposed to sub-BF activity in the cochlear base. The observation that the lateral and RL regions move approximately linearly sub-BF indicates that linear forces dominate non-linear OHC-based forces on these components at sub-BF frequencies. A complex difference analysis was performed to reveal the internal motion of the OHC/DC region and showed that amplitude structure and phase shifts in the directly measured OHC/DC motion emerge due to the internal OHC/DC motion destructively interfering with BM motion.

Nanoscale vibration could promote tenogenic differentiation of umbilical cord mesenchymal stem cells.

Regulation of mesenchymal stem cell (MSC) fate for targeted cell therapy applications has been a subject of interest, particularly for tissues such as tendons that possess a marginal regenerative capacity. Control of MSCs' fate into the tendon-specific lineage has mainly been achieved by implementation of chemical growth factors. Mechanical stimuli or 3-dimensional (D) scaffolds have been used as an additional tool for the differentiation of MSCs into tenocytes, but oftentimes, they require a sophisticated bioreactor or a complex scaffold fabrication technique which reduces the feasibility of the proposed method to be used in practice. Here, we used nanovibration to induce the differentiation of MSCs toward the tenogenic fate solely by the use of nanovibration and without the need for growth factors or complex scaffolds. MSCs were cultured on 2D cell culture dishes that were connected to piezo ceramic arrays to apply nanovibration (30-80nm and 1kHz frequency) over 7 and 14 d. We observed that nanovibration resulted in significant overexpression of tendon-related markers in both gene expression and protein expression levels, while there was no significant differentiation into adipose and cartilage lineages. These findings could be of assistance in the mechanoregulation of MSCs for stem cell engineering and regenerative medicine applications.

Integrated transparent surface acoustic wave technology for active de-fogging and icing protection on glass

There have been great concerns on poor visibility and hazardous issues due to fogging and ice/frost formation on glass surfaces of windshields, windows of vehicles/airplanes, and solar panels. Existing methods for their monitoring and removal include those active ones (such as using resistance heating) or passive ones (such as using surface icephobic treatments), which are not always applicable, effective or reliable. In this study, we proposed a novel strategy by implementing transparent thin film surface acoustic wave (SAW) devices by directly coating ZnO films onto glass substrate and studied their de-fogging, active anti-icing and de-icing mechanisms using the SAW technology. Effects of powers and wavelengths of SAW devices were investigated and influences of acousto-heating and surface hydrophobic treatments were evaluated. Results showed that de-fogging time was dramatically decreased with the increase of SAW powers when the thin film-based SAW devices were exposed to humid air flow for different durations. The icing accretion was significantly delayed under the applied SAW agitation, and SAW application has also effectively promoted de-icing on glass substrate, due to the interfacial nanoscale vibration and localized heating effect.

Vibration-induced nanoscale friction modulation on piezoelectric materials

Mechanical vibration, as an alternative of application of solid/liquid lubricants, has been an effective means to modulate friction at the macroscale. Recently, atomic force microscopy (AFM) experiments and model simulations also suggest a similar vibration-induced friction reduction effect for nanoscale contact interfaces, although an additional external vibration source is typically needed to excite the system. Here, by introducing a piezoelectric thin film along the contact interface, we demonstrate that friction measured by a conductive AFM probe can be significantly reduced (more than 70%) when an alternating current (AC) voltage is applied. Such real-time friction modulation is achieved owing to the localized nanoscale vibration originating from the intrinsic inverse piezoelectric effect, and is applicable for various material combinations. Assisted by analysis with the Prandtl—Tomlinson (P—T) friction model, our experimental results suggest that there exists an approximately linear correlation between the vibrational amplitude and the relative factor for perturbation of sliding energy corrugation. This work offers a viable strategy for realizing active friction modulation for small-scale interfaces without the need of additional vibration source or global excitation that may adversely impact device functionalities.

Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures

Models of bone remodelling could be useful in drug discovery, particularly if the model is one that replicates bone regeneration with reduction in osteoclast activity. Here we use nanovibrational stimulation to achieve this in a 3D co-culture of primary human osteoprogenitor and osteoclast progenitor cells. We show that 1000 Hz frequency, 40 nm amplitude vibration reduces osteoclast formation and activity in human mononuclear CD14+ blood cells. Additionally, this nanoscale vibration both enhances osteogenesis and reduces osteoclastogenesis in a co-culture of primary human bone marrow stromal cells and bone marrow hematopoietic cells. Further, we use metabolomics to identify Akt (protein kinase C) as a potential mediator. Akt is known to be involved in bone differentiation via transforming growth factor beta 1 (TGFβ1) and bone morphogenetic protein 2 (BMP2) and it has been implicated in reduced osteoclast activity via Guanine nucleotide-binding protein subunit α13 (Gα13). With further validation, our nanovibrational bioreactor could be used to help provide humanised 3D models for drug screening.

Reduction of Ice Adhesion Using Surface Acoustic Waves: Nanoscale Vibration and Interface Heating Effects.

Ice accumulation causes great risks to aircraft, electric power lines, and wind-turbine blades. For the ice accumulation on structural surfaces, ice adhesion force is a crucial factor, which generally has two main sources, for exampple, electrostatic force and mechanical interlocking. Herein, we present that surface acoustic waves (SAWs) can be applied to minimize ice adhesion by simultaneously reducing electrostatic force and mechanical interlocking, and generating interface heating effect. A theoretical model of ice adhesion considering the effect of SAWs is first established. Experimental studies proved that the combination of nanoscale vibration and interface heating effects lead to the reduction of ice adhesion on the substrate. With the increase of SAW power, the electrostatic force decreases due to the increase of dipole spacings, which is mainly attributed to the SAW induced nanoscale surface vibration. The interface heating effect leads to the transition of the locally interfacial contact phase from solid-solid to solid-liquid, hence reducing the mechanical interlocking of ice. This study presents a strategy of using SAWs device for ice adhesion reduction, and results show a considerable potential for application in deicing.

Acoustic-Excitation Optical Coherence Vibrometer for Real-Time Microstructure Vibration Measurement and Modal Analysis

An acoustic-excitation optical coherence vibrometer (AE-OCV) is proposed for noncontact microstructure vibration measurement and modal analysis. The vibration dynamic parameters of the microstructure can be captured with a subnanometer displacement resolution by the proposed AE-OCV using spectral wavelength calibration algorithm and spectral center correction method. The natural frequency of the microstructure could also be obtained by the developed noncontact AE-OCV system using the swept-frequency acoustic excitation method. Furthermore, a home-made spectrometer based on a high-speed linear camera could provide the system with a frequency measurement range of 0–50 kHz. The developed AE-OCV has an excellent performance in microstructures vibration measurement and modal analysis, especially for high-frequency and nanoscale vibration measurements. It is of great significance to the design of the microelectromechanical system (MEMS), such as the noncontact dynamic measurement of cantilevered beams in atomic force microscopy.

Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration

Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (nanokicking) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60nm) were imposed on P.aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 and 4000Hzat 24h. The optimal reduction of biofilm formation was observed at 1kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24hat 1kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.

Homodyne Laser Vibrometer With Detectability of Nanoscale Vibration and Adaptability to Reflectivity

Homodyne laser vibrometer plays an essential role in the precision vibration measurement, but its application is restricted when measuring subfringe vibration and targets with various reflectivities. In order to achieve the nanoscale vibration measurement and to adapt to various reflectivities, an enhanced homodyne laser vibrometer is presented in this paper. First, an active vibration mechanism (AVM) based on a flexure hinge stimulated by a piezoelectric transducer (PZT) is introduced into the reference arm, with which the parameters for nonlinear error correction can be predetermined, and thereby the measurement of vibration less than quarter wavelength is feasible. Second, an automatic gain module is employed to optimize the intensity of the interference signals, which significantly improves the adaptability to target reflectivity. The experimental results show that the laser vibrometer proposed is capable of measuring vibration with amplitude down to 2 nm and vibration with surface reflectivity down to 0.08%.

Large-range nanoscale self-mixing interferometer based on multiple reflections and even-power fast algorithm

We propose a novel integrated measuring technique (IMT) in self-mixing interference (SMI) for measuring the nanoscale vibration, which is realized by combining multiple reflections with the even-power fast algorithm Theoretical analysis show that the fringe precision could be improved to λ/(2n+3∗G). The experiments with amplitudes of 680 nm and 100 nm have been carried out to verify the feasibility of IMT. The minimum measurement error is 1.34 nm, which is within the theoretical error range. Based on the experimental results, the alignment of the external mirror and the repeatability of the experiment are also discussed. Given its high precision, compact structure, and large measurement range, the proposed method has potential applications in the field of high precision nanometer measurement.

Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans.

Physical forces are transduced into chemical reactions, thereby ultimately making a large impact on the whole-animal level phenotypes such as homeostasis, development and behavior. To understand mechano-chemical transduction, mechanical input should be quantitatively delivered with controllable vibration properties–frequency, amplitude and duration, and its chemical output should be noninvasively quantified in an unconstrained animal. However, such an experimental system has not been established so far. Here, we develop a noninvasive and unconstrained mechanochemical imaging microscopy. This microscopy enables us to evoke nano-scale nonlocalized vibrations with controllable vibration properties using a piezoelectric acoustic transducer system and quantify calcium response of a freely moving C. elegans at a single cell resolution. Using this microscopy, we clearly detected the calcium response of a single interneuron during C. elegans escape response to nano-scale vibration. Thus, this microscopy will facilitate understanding of in vivo mechanochemical physiology in the future.

Multiple self-mixing interferometry algorithm based on phase modulation for vibration measurement

A novel algorithm based on a phase modulation and demodulation technique for multiple self-mixing interferometry was proposed in this paper. This algorithm can measure nanoscale vibration in real time and enhance the measurement resolution of a multiple self-mixing interferometer twofold compared with that of a self-mixing interferometer. In this paper, the principles of the method were described in detail, and a phase equation for multiple self-mixing interferometry was derived. Experiments were confirmed by conducting a series of experiments with different amplitudes, different signal-to-noise ratios, and different vibration waveforms. The experimental results showed that the algorithm can rapidly demodulate vibration in real time and indicated good agreement between theory and experiment.

Nonlocal vibration and buckling analysis of single and multi-layered graphene sheets using finite strip method including van der Waals effects

Detailed studies on the nanoscale vibration and buckling characteristics of rectangular single and multi-layered graphene sheets (SLGSs and MLGSs) are carried out using semi-analytical finite strip method (FSM), based on the classical plate theory (CPT). The displacement functions of the sheets are evaluated using continuous harmonic function series which satisfy the boundary conditions in one direction and a piecewise interpolation polynomial in the other direction. Nonlocal continuum mechanics is employed to derive the differential equation of the system. The weighted residual method is employed to obtain stiffness, stability and mass matrices of the graphene sheets. The effects of van der Waals (vdW) forces which are present as bonding forces between the layers are considered in the stiffness matrix of the system. The analysis of MLGSs is much more complex due to the influence of vdW forces. The mechanical properties of the graphene sheet are assumed in two ways as orthotropic or isotropic materials. A matrix eigenvalue problem is solved to find the natural frequency and critical stress of GSs subjected to different types of in-plane loadings including uniform and non-uniform uniaxial loadings. The accuracy of the proposed model is validated by comparing the results with those reported by the available references. Furthermore, a comprehensive parametric study is performed to investigate the effects of various parameters such as boundary conditions, nonlocal parameter, aspect ratio and the type of loading on the results.

Nanoscale vibration characteristics of multi-layered graphene sheets

A continuum-based plate model is proposed to study the nanoscale vibration characteristics of multi-layered graphene sheets (MLGSs) that are increasingly being proposed for important engineering applications such as THz resonators. Generalized Differential Quadrature (GDQ) method is used to predict the natural frequencies and their associated vibration modes of single-layered and triple-layered graphene sheets, as well as general MLGSs. Numerical simulations are carried out to examine the effects of van der Waals (vdW) interactions, which are present as bonding forces between the layers, on nanoscale vibration natural frequencies and their mode shapes. The results show that for a general MLGSs, vibration modes can be classified into 3 families—lower classical synchronized modes which are independent of van der Waals forces, middle van der Waals enhanced modes which are largely determined by the presence of van der Waals interactions and higher mixed modes which are combinations of the classical synchronized modes and van der Waals enhanced modes. Detailed characterizations of these modes from their derived mode shapes have been achieved for the typical case of triple-layered GSs, as well as general MLGSs. Effects of different boundary conditions, aspect ratios and the number of layers on nanoscale vibration properties have been examined in detail. The results presented in this paper, for the first time, provide accurate and wholesome studies and characterizations on the interesting nanoscale vibration properties of multi-layered graphene sheets and the results obtained will certainly be useful to those who are concerned with the dynamics of graphene sheets which are increasingly being deployed for various innovative engineering applications.

Multiscale Analysis of Cutting Force During Nano-Scale Vibration Assisted Machining

Multiscale Analysis of Cutting Force During Nano-Scale Vibration Assisted Machining

Nanoscale vibration characterization of multi-layered graphene sheets embedded in an elastic medium

Detailed studies on the nanoscale vibration characteristics of multi-layered graphene sheets (MLGSs) that are embedded in an elastic medium are carried out using continuum-based modelling and Generalized Differential Quadrature (GDQ) method. Natural frequencies and their associated vibration modes of practical interest of single-layered and triple-layered graphene sheets, as well as general MLGSs that are embedded in an elastic medium are established. Numerical simulations are conducted to examine the effects of van der Waals (vdW) interactions, which are present as bonding forces between the layers, on nanoscale vibration natural frequencies and their mode shapes. The results show that for a general MLGSs embedded in an elastic medium, vibration modes can in general be classified into three families – lower classical synchronized modes which are independent of van der Waals forces and are somewhat sensitive to the surrounding elastic medium, middle van der Waals enhanced modes which are largely determined by the presence of van der Waals interactions and are hence less sensitive to the changes of the surrounding elastic medium, and higher mixed modes which are combinations of classical synchronized modes and van der Waals enhanced modes. Detailed characterizations of these modes from their derived mode shapes have been achieved for the typical case of an embedded triple-layered GSs, as well as general embedded MLGSs. Effects of Winkler modulus K W , the shear layer modulus G b , different boundary conditions, aspect ratio β and the number L of graphene layers on nanoscale vibration properties have been examined in detail. The results presented in this paper, for the first time, provide accurate and wholesome studies and characterizations on the interesting nanoscale vibration properties of multi-layered graphene sheets embedded in an elastic medium and the results obtained will certainly be useful to those who are concerned with the dynamics of embedded graphene sheets which are increasingly being deployed for various innovative engineering applications such as nano-electro-mechanical systems (NEMS).

Nanoscale vibration analysis of embedded multi-layered graphene sheets under various boundary conditions

The present article deals with the vibrational analysis of multi-layered graphene sheets with different boundary conditions amongst sheets. An elastic multiple-plate model is utilized in which the nested plates are coupled with each other through the van der Waals interlayer force. The interaction of van der Waals forces between adjacent and non-adjacent layers and the reaction from the surrounding media are included in the Reissner–Mindlin-type field equations on which the theoretical formulation is based. The set of coupled equations of motion for the multi-layered graphene sheets is then solved by the generalized differential quadrature method. The numerical analysis presented herein provides the possibility of considering various combinations of layerwise boundary conditions in a multi-layered graphene sheet. Based on exact solution, explicit formulas for the frequencies of a double-layered graphene sheet with all edges simply supported are also obtained. The results of the present numerical solution are shown to be in excellent agreement with those of exact solution for simply supported graphene sheets.

Cu-Al-Ni-SMA-Based High-Damping Composites

Recently, absorption of vibration energy by mechanical damping has attracted much attention in several fields such as vibration reduction in aircraft and automotive industries, nanoscale vibration isolations in high-precision electronics, building protection in civil engineering, etc. Typically, the most used high-damping materials are based on polymers due to their viscoelastic behavior. However, polymeric materials usually show a low elastic modulus and are not stable at relatively low temperatures (≈323 K). Therefore, alternative materials for damping applications are needed. In particular, shape memory alloys (SMAs), which intrinsically present high-damping capacity thanks to the dissipative hysteretic movement of interfaces under external stresses, are very good candidates for high-damping applications. A completely new approach was applied to produce high-damping composites with relatively high stiffness. Cu-Al-Ni shape memory alloy powders were embedded with metallic matrices of pure In, a In-10wt.%Sn alloy and In-Sn eutectic alloy. The production methodology is described. The composite microstructures and damping properties were characterized. A good particle distribution of the Cu-Al-Ni particles in the matrices was observed. The composites exhibit very high damping capacities in relatively wide temperature ranges. The methodology introduced provides versatility to control the temperature of maximum damping by adjusting the shape memory alloy composition.

Mechanical spectroscopy measurements on SMA high-damping composites

High-damping materials have attracted much attention to solve problems such as acoustic pollution, nano-scale vibration isolations in electronic industry, vibration damping in civil engineering, etc. Shape memory alloys (SMAs), which intrinsically present high-damping capacity, are considered as alternative materials to the traditionally used polymeric ones, because they present better mechanical properties at moderate temperatures. A new kind of high-damping metal matrix composites has been produced by embedding a relatively high amount (approximately 60 vol.%) of Cu–Al–Ni SMA particles with metallic matrices (In, In + Sn). The damping properties have been characterized by mechanical spectroscopy, using an inverted torsion pendulum, as a function of temperature (150–400 K), frequency (0.01–3 Hz), and strain amplitude (2 × 10 −5 to 8 × 10 −5). The materials exhibit internal friction higher than 0.5 in a relatively wide temperature range. The ability of matching the temperature of maximum damping, through the composition of the SMA, opens new possibilities for designing high-damping materials for specific applications.