Selenium Nanocomposites Research Articles

Design, fabrication, and evaluation of keratin and pectin incorporated supramolecular structured zero-oxidation state selenium nanogel blended 3D printed transdermal patch

This study investigates the synthesis of selenium nanoparticles (SeNPs), owing to the low cost and abundance of selenium. However, the toxicity of SeNP prompts the development of a selenium nanocomposite (SeNC) containing pectin, keratin, and ferulic acid to improve the bioactivity of Se[0]. Further, incorporating the SeNC in a suitable formulation for drug delivery as a transdermal patch was worth studying. Accordingly, various analytical techniques were used to characterize the SeNPs and the SeNC, confirming successful synthesis and encapsulation. The SeNC exhibited notable particle size of 448.2 ± 50.2 nm, high encapsulation efficiency (98.90 % ± 2.4 %), 28.1 ± 0.45 drug loading, and sustained drug release at pH 5.5. Zeta potential and XPS confirmed the zero-oxidation state. The supramolecular structure was evident from spectral analysis endorsing the semi-crystalline nature of the SeNC and SEM images showcasing flower-shaped structures. Further, the SeNC demonstrated sustained drug release (approx. 22 % at 48 h) and wound-healing potential in L929 fibroblast cells. Subsequently, the SeNC loaded into a gelling agent exhibited shear thinning properties and improved drug release by nearly 58 %. A 3D printed reservoir-type transdermal patch was developed utilizing the SeNC-loaded gel, surpassing commercially available patches in characteristics such as % moisture uptake, tensile strength, and hydrophobicity. The patch, evaluated through permeation studies and CAM assay, exhibited controlled drug release and angiogenic properties for enhanced wound healing. The study concludes that this patch can serve as a smart dressing with tailored functionality for different wound stages, offering a promising novel drug delivery system for wound healing.

Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites

BackgroundBacterial contamination poses a high risk to the successful establishment and maintenance of plant tissue cultures. The aim of this study was to identify the isolates representing the frequent bacterial contaminants of Prunus rootstock tissue cultures and to determine the most effective concentration of nanomaterials for Curtobacterium sp. strain A7_M15 elimination without a negative impact on explants.ResultsSix Curtobacterium sp. strains were isolated and identified, and the whole-genome sequence was obtained for strain A7_M15. Two nanocomposites, reduced graphene oxide–copper–silver and silver–selenium, with the highest bactericidal activity were selected for elimination of Curtobacterium sp. contamination in Gisela 5 rootstock tissue cultures. Both nanocomposites showed 100% inhibition of bacterial plaque formation on culture medium at concentrations of 100, 200 and 400 mg L-1 Ag (2 ×–8 × MBC). The quantity of Curtobacterium sp. on culture medium assessed using cfu enumeration was reduced by 92% and 74% in comparison to the positive control after treatment with reduced graphene oxide–silver–copper and silver–selenium at a concentration of 200 mg L-1 Ag, respectively. None of the tested concentrations resulted in a decrease in Curtobacterium sp. quantity in explants. Curtobacterium sp. was detected in donor Gisela 5 plants, indicating an endophytic character of this bacterium. The dry weight of explants was not negatively affected by the application of nanocomposites regardless of concentration, and no detrimental effect of either nanocomposite at 100 or 200 mg L-1 Ag on the surface covered by plants was observed.ConclusionsReduced graphene oxide–silver–copper and silver–selenium nanocomposites at 200 mg L-1 Ag effectively limited the Curtobacterium sp. presence in micropropagated Prunus rootstock without causing phytotoxicity; therefore, those treatments could be offered as prevention with a high activity against bacterial contamination in plant tissue cultures.Graphical

Effect of Biogenic Selenium Nanocomposite on Okra and Metagenomic Analysis of Bacterial Community Associated with Rhizosphere and Phyllosphere

Effect of Biogenic Selenium Nanocomposite on Okra and Metagenomic Analysis of Bacterial Community Associated with Rhizosphere and Phyllosphere

Anticancer activity of lactoferrin-coated biosynthesized selenium nanoparticles for combating different human cancer cells via mediating apoptotic effects

The present study aims to develop a novel nanocombination with high selectivity against several invasive cancer cells, sparing normal cells and tissues. Bovine lactoferrin (bLF) has recently captured the interest of numerous medical fields owing to its biological activities and well-known immunomodulatory effects. BLF is an ideal protein to be encapsulated or adsorbed into selenium nanocomposites (Se NPs) in order to produce stable nanocombinations with potent anticancer effects and improved immunological functions. The biosynthesis of the functionalized Se NPs was achieved using Rhodotorula sp. strain MZ312359 via a simultaneous bio-reduction approach to selenium sodium salts. The physicochemical properties of Se NPs using SEM, TEM, FTIR, UV Vis, XRD, and EDX confirmed the formation of uniform agglomerated spheres with a size of 18–40 nm. Se NPs were successfully embedded in apo-LF (ALF), forming a novel nanocombination of ALF-Se NPs with a spherical shape and an average nanosize of less than 200 nm. The developed ALF-Se NPs significantly displayed an effective anti-proliferation efficiency against many cancer cells, including MCF-7, HepG-2, and Caco-2 cell lines, as compared to Se NPs and ALF in free forms. ALF-Se NPs showed a significant selectivity impact (> 64) against all treated cancer cells at IC50 63.10 ≤ μg/mL, as well as the strongest upregulation of p53 and suppression of Bcl-2, MMP-9, and VEGF genes. Besides, ALF-Se NPs were able to show the maximum activation of transcrition of key redox mediator (Nrf2) with suppression in reactive oxygen species (ROS) levels inside all treated cancer cells. This study demonstrates that this novel nanocombination of ALF-Se NPs has superior selectivity and apoptosis-mediating anticancer activity over free ALF or individual form of Se NPs.

Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of Pectobacterium carotovorum

In recent decades, the use of nanocomposites (NCs) in crop production has been actively studied. We have previously shown that chemically synthesized selenium nanocomposites (Se NCs) based on natural polymeric matrices of arabinogalactan (Se/AG NCs), carrageenan (Se/CAR NCs), and starch (Se/ST NCs) stimulated the growth and development of potatoes in vitro and had an antibacterial effect against the phytopathogen Clavibacter sepedonicus. It is important to confirm that the results obtained in the laboratory are also reproducible in the field conditions of crop cultivation. In addition, the applications of NCs can be expanded if a wider range of their biological activity is revealed, and the effect on other types of cultivated plants and phytopathogens is shown. In this regard, the purpose of this research was to study the effect of nanopriming with Se NCs on the productivity of potatoes in a field experiment, the germination of soybeans, and the viability of the phytopathogen Pectobacterium carotovorum under in vitro conditions. The study included the use of traditional methods of conducting a field experiment in natural growing conditions, also using microbiological cultures and studying the bacterial profile from the endosphere of potato tubers by the imprint method, germinating Glycine max L. soybean seeds after nanopriming in Petri dishes, followed by the measurement of morphometric and biochemical parameters such as the activity of antioxidant enzymes and content of diene conjugates. Based on the results of field experiments, it was found that the preplant treatment of tubers with Se/AG and Se/CAR NCs stimulated a significant increase in the number of stems in potato plants and tubers per plant, but for Se/AG NC, the increase was observed only in 2020 and 2022, and the stimulating effect of Se/AG and Se/CAR NCs on the weight of tubers was observed only in 2020. In the yield structure, the proportion of marketable seed tubers was increased under the influence of all Se NCs. Endosphere cultures from tubers showed that bacteria were present in only 56% of the tubers in the control, while 78% of the tubers obtained from plants grown from seed tubers treated with Se/AG NC and 100% of the tubers from plants grown from seed tubers treated with Se/ST and Se/CAR NCs contained bacteria. The bacteria were represented by both spore-forming Gram-positive and Gram-negative bacteria of various sizes. The results presented in this article and previously published data showed that the maximum diversity of bacteria was observed after treatment with Se/ST NC. Experiments on potatoes showed that Se/AG NC had the greatest biological effect. In addition, the growth-stimulating effect of these NCs on the biomass of the roots of soybean seedlings was shown. It activated the antioxidant enzymes and reduced the level of diene conjugates (DCs), the amount of which increased significantly after seed biopriming with the phytopathogen Pectobacterium carotovorum. The antibacterial effect of Se/AG NC in relation to the P. carotovorum was observed as a reduction in the growth of the bacterial culture, biofilm formation, and dehydrogenase activity of cells. Thus, it has been shown that Se/AG NC has a stimulating effect on such agriculturally important crops as potatoes and soybeans, as well as an antibacterial effect against Gram-negative and Gram-positive phytopathogenic bacteria.

Mushroom-Derived Novel Selenium Nanocomposites' Effects on Potato Plant Growth and Tuber Germination.

Multicomponent materials, where nanosized selenium (Se) is dispersed in polymer matrices, present as polymer nanocomposites (NCs), namely, selenium polymer nanocomposites (SeNCs). Selenium as an inorganic nanofiller in NCs has been extensively studied for its biological activity. More ecologically safe and beneficial approaches to obtain Se-based products are the current challenge. Biopolymers have attained great attention with perspective multifunctional and high-performance NCs exhibiting low environmental impact with unique properties, being abundantly available, renewable, and eco-friendly. Composites based on polysaccharides, including beta-glucans from edible and medicinal mushrooms, are bioactive, biocompatible, biodegradable, and have exhibited innovative potential. We synthesized SeNCs on the basis of the extracellular polysaccharides of several medicinal mushrooms. The influence of bio-composites from mushrooms on potato plant growth and tuber germination were studied in two potato cultivars: Lukyanovsky and Lugovskoi. Bio-composites based on Grifola umbellata demonstrated the strongest positive effect on the number of leaves and plant height in both cultivars, without negative effect on biomass of the vegetative part. Treatment of the potato tubers with SeNC from Gr. umbellata also significantly increased germ length. Potato plants exposed to Se-bio-composite from Ganoderma lucidum SIE1303 experienced an increase in the potato vegetative biomass by up to 55% versus the control. We found earlier that this bio-composite was the most efficient against biofilm formation by the potato ring rot causative agent Clavibacter sepedonicus (Cms). Bio-composites based on Pleurotus ostreatus promoted increase in the potato root biomass in the Lugovskoi cultivar by up to 79% versus the control. The phytostimulating ability of mushroom-based Se-containing bio-composites, together with their anti-phytopathogenic activity, testifies in favor of the bifunctional mode of action of these Se-biopreparations. The application of stimulatory green SeNCs for growth enhancement could be used to increase crop yield. Thus, by combining myco-nanotechnology with the intrinsic biological activity of selenium, an unexpectedly efficient tool for possible applications of SeNCs could be identified.

Effect of Selenium Nanocomposites Based on Natural Polymer Matrices on the Biomass and Storage of Potato Tubers in a Field Experiment

The effect of chemically synthesized selenium (Se) nanocomposites (NCs) based on the natural polymer matrices arabinogalactan (AG), carrageenan (CAR), and starch (ST) on potato tuber traits, storage, and crop structure was studied in a field trial. Parental potato tubers were sprayed by Se/AG NC, Se/ST NC, and Se/CAR NC 14 days before planting in the field. The results showed that Se/AG NC and Se/CAR NC increased the number and weight of tubers in the first generation (F1) obtained from the plants grown from the treated tubers. It was found that Se/AG NC and Se/ST NC decreased the median weight of shoots after 230 days of storage of the F1 tubers, preventing their premature germination, and Se/AG NC decreased the number of rotten tubers. All three Se NCs significantly improved the storage by increasing the number of healthy scab-, dry-pitted-rot-, and wireworm-free tubers in the F1 after 230-day-long storage, except Se/CAR NC regarding dry pitted rot. Selenium/ST NC significantly increased the number of tubers, and Se/CAR NC their mass, and both decreased the number of rotten tubers in the second generation (F2). Selenium NCs affected crop structure in both generations.

Comparing The Effects of Selenium Nanoparticles and Selenium Nanocomposites on Food Intake and Anxiety-like Behaviors

Background & Objective: Selenium nanoparticles (Se -NPs) and selenium nanocomposites (Se -NCs) have different biological effects. Therefore, the present study aimed to compare the effects of selenium nanoparticles and selenium nanocomposites (Se –NCs) on anxiety, food intake, and brain histology of rats. Materials & Methods: Thirty-two male adult Wistar rats were randomly and equally divided into four groups. The control group received saline, the Selenium powder group received 1 mg/kg /day selenium powder orally for 21 days, and the selenium nanoparticle group received three weeks of oral gavage of Se –NPs. The selenium nanocomposites group received three weeks of oral gavage of nanocomposites. Finally, cumulative food consumption and anxiety-like behaviors were assessed, and, after that, rats were bled and sacrificed for further biochemical and histopathological investigations. Results: Oral administration of Se powder at a dose of 1 mg/kg /day for 21 days had no significant effect on the brain superoxide dismutase (SOD), catalase (CAT) activities. There was also no significant change in the levels of brain glutathione concentration (GSH), brain MDA, and behavioral parameters. Selenium nanoparticles also showed no significant alterations in brain biochemical parameters, behavioral effects, and brain histology. The oral administration of Selenium nanocomposites significantly increased brain superoxide dismutase, catalase, and reduced glutathione content and had positive effects on behavioral parameters. Conclusion: The present study showed that Se -NCs have behavioral effects and could induce significant biochemical changes in brain oxidative status.

СЕЛЕН В НАНОФОРМЕ: ТОКСИЧНОСТЬ И БЕЗОПАСНОСТЬ

The review is devoted to the compilation of data on the toxicity and safety of using nanosized forms of selenium. It is shown that in nature selenium exists mainly in the form of selenate (Se6+), selenite (Se4+), selenide (Se2–) and elemen-tal selenium (Se0), while the latter is insoluble in aqueous media, less toxic and biologically inert. Elemental selenium in the form of nanosized particles is not only biocompatible, but also has antitumor and antimicrobial activity. It was shown that elemental selenium in the form of nanoparticles can modulate the activity of the antioxidant and detoxification systems. A data on the dose-dependent effect of selenium in nanosized form was presented. It was demonstrated that selenium nanoparticles in high concentrations (above 2 mg of Se per 1 kg of animal weight) can cause the development of selenium-induced toxicity in mammals. It was shown that elemental selenium in the form of nanoparticles can affect immunoregulation, reproductive func-tion, kidney and liver function, can modulate the activity of the antioxidant and detoxification systems, and in high concentrations (above 2 mg of Se per 1 kg of animal weight) it can cause the development of selenium-induced toxicity both in mammals and fish. At the same time, it was shown that for fish selenium nanoparticles are more toxic than inorganic selenium and cause a more acute reaction of the body to exposure to even low concentrations, which is possibly associated with hyperaccumulation of selenium in tissues. This fact once again reminds us of the need to take into account the problems of ecotoxicity of selenium nanocomposites.

Morphofunctional changes in the tissue of the brain, liver and kidneys of white rats under the influence of selenium nanocomposite encapsulated in the polymer matrix of arabinogalactan

Introduction. Due to their high biocompatibility, substances based on nanosized selenium particles, encapsulated in natural or synthetic polymer matrices, are promising materials for the creation of biomedical preparations of diagnostic and therapeutic value. Selenium nanoparticles are successfully used in the diagnosis of various types of cancer. In addition to the diagnostic value, selenium nanoparticles have their own prophylactic and oncological effect. This paper presents the results of a study of the toxicity of the Se nanocomposite encapsulated in the polymer matrix of arabinogalactan (SeAG). The emergence and development of the pathological process in the tissue of the brain, liver and kidneys during subacute administration of this nanocomposite was studied.Materials and methods. Twenty white outbred male rats weighing 200–220 g were used in the work. Animals were orally administered a solution of the selenium nanocomposite at a dose of 500 μg per kilogram of animal body weight for 10 days. Then, using the methods of histological analysis, the severity of the biological response of the organism to the introduction of this nanocomposite was assessed. An analysis of the state of the tissue of the liver, kidneys and the sensorimotor cortex of the brain was carried out.Results. With the intragastric administration of this drug, there is stasis of blood in the portal tracts, a pronounced macrophage reaction and diapedesis of leukocytes in the liver tissue. There is a decrease in the number of normal neurons per unit area, a decrease in the number of astroglia cells and an increase in the number of degeneratively altered neurons in the tissue of the sensorimotor cortex. There is also an increase in connective tissue in the cortex of the kidney, with the formation of fibrosis and a decrease in the area of the Shumlyansky – Bowman capsule.Conclusion. The effect of the investigated nanocomposite is characterized by the development of a pronounced pathological process in the central nervous and hepatorenal systems of the body.

Comparing the Histopathological Effects of Selenium Nanoparticles and Selenium Nanocomposites in Rat Models

Background: Selenium nanoparticles (Se NPs) and selenium nanocomposites (Se NCs) have different biological effects. The current study aimed to compare the effects of newly synthesized Se NPs and Se NCs on biochemical and histopathological parameters of rats. The synthesized Se NPs were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDAX), and scanning electron microscopy (SEM) techniques. Materials and Methods: Adult male Wistar rats were divided into four equal groups to examine the biological effects of Se NPs. Control rats received saline intraperitoneally while experimental rats were received four-week intraperitoneal injections of Se powder, Se NPs, and Se NCs at the dose of (0.4 mg/kg). After four weeks, serum was obtained by the conventional methods, and then rats were sacrificed to separate liver, kidney, and testis tissues for histopathological examinations. Results: The intraperitoneal injection of Se powder caused significant elevations in serum liver enzyme levels, serum blood urea nitrogen (BUN) lipid peroxidation, and serum creatinine levels (P<0.05). The histopathological investigations showed necrosis and fatty change in liver. Kidney sections showed cytoplasmic vacuolation and hyaline casts, and the testis sections showed degeneration of seminiferous tubules. Se NPs intraperitoneal injections at a dose of 0.4 mg/kg caused no significant effects on liver enzymes, malondialdehyde (MDA) content, and histopathological changes while significantly increased serum BUN and creatinine levels (P<0.05). The group treated with Se NCs showed normal biochemical and histopathological parameters (P<0.05). Conclusion: The current study proved the toxicity of Se powder; however, nano-formulations of Se showed fewer side effects.

Selenium Nanocomposites in Natural Matrices as Potato Recovery Agent.

The paper presents a study of the effect of chemically synthesized selenium nanocomposites (Se NCs) in natural polymer matrices arabinogalactan (AG) and starch (ST) on the viability of the potato ring rot pathogen Clavibacter sepedonicus (Cms), potato plants in vitro, and the soil bacterium Rhodococcus erythropolis. It was found that the studied Se NCs have an antibacterial effect against the phytopathogenic Cms, reducing its growth rate and ability to form biofilms. It was revealed that Se NC based on AG (Se/AG NC) stimulated the growth and development of potato plants in vitro as well as their root formation. At the same time, Se did not accumulate in potato tissues after the treatment of plants with Se NCs. The safety of the Se NCs was also confirmed by the absence of a negative effect on the growth and biofilm formation of the soil bacterium R. erythropolis. The obtained results indicate that Se NCs are promising environmentally safe agents for the protection and recovery of cultivated plants from phytopathogenic bacteria.

Co-delivery of ampicillin and β-lactamase inhibitor by selenium nanocomposite to achieve synergistic anti-infective efficiency through overcoming multidrug resistance

Bacterial infective diseases due to antibiotic resistance remain a great challenge in clinic. Design of novel antibacterial drugs with high efficiency and low side effects to overcome antibiotic resistance is urgently needed. Herein, M-Cs (mannose- modified chitosan) modified selenium (Se) nanoparticles to co-deliver ampicillin (Ap) and β-lactamase inhibitor were synthesized and characterized (SAMCP NPs). Mannose modification endowed SAMCP NPs with enhanced recognition of bacteria by FimH lectin. Co-delivery of ampicillin and β-lactamase inhibitor (3-carboxyphenylboronic acid, Pba) by selenium nanocomposite (30 μg/mL) effectively inhibited β-lactamase activity, which synergistically enhanced ampicillin-induced antibacterial activity against ampicillin-resistant Escherichia coli (E. coli (R)) in vitro by suppression of AcrAB-TolC efflux pump system through eliminating ROS. SAMCP NPs (5 mg/kg) in vivo also showed synergistic anti-infective efficiency by inhibiting E. coli (R) infection-induced inflammatory response, cell apoptosis and regulating multiple signal pathways, and eventually promoted wound healing of mice. Importantly, SAMCP NPs displayed low toxicity and low side effects in vivo. Our findings validated our rational design that co-delivery of ampicillin and β-lactamase inhibitor by selenium nanocomposite to overcome multidrug resistance could be a highly efficient way to achieve antibacterial synergy.

A Selenium Nanocomposite Protects the Mouse Brain from Oxidative Injury Following Intracerebral Hemorrhage.

BackgroundIntracerebral hemorrhage (ICH) is a common neurological crisis leading to high mortality and morbidity. Oxidative stress-induced secondary injury plays a critical role in neurological deterioration. Previously, we synthesized a porous Se@SiO2 nanocomposite and identified their therapeutic role in osteonecrosis of the femoral head. Whether this nanocomposite is neuroprotective remains to be elucidated.MethodsA porous Se@SiO2 nanocomposite was synthesized, and its biosafety was determined using a CCK-8 assay. The neuroprotective effect was evaluated by TUNEL staining, and intracellular ROS were detected with a DCFH-DA probe in SH-SY5Y cells exposed to hemin. Furthermore, the effect of the nanocomposite on cell apoptosis, brain edema and blood–brain barrier permeability were evaluated in a collagenase-induced ICH mouse model. The potential mechanism was also explored.ResultsThe results demonstrated that Se@SiO2 treatment significantly improved neurological function, increased glutathione peroxidase activity and downregulated malonaldehyde levels. The proportion of apoptotic cells, brain edema and blood–brain barrier permeability were reduced significantly in ICH mice treated with Se@SiO2 compared to vehicle-treated mice. In vitro, Se@SiO2 protected SH-SY5Y cells from hemin-induced apoptosis by preventing intracellular reactive oxygen species accumulation.ConclusionThese results suggested that the porous Se@SiO2 nanocomposite exerted neuroprotection by suppressing oxidative stress. Se@SiO2 may be a potential candidate for the clinical treatment of ICH and oxidative stress-related brain injuries.

Влияние нанокомпозитов селена в природных полимерных матрицах на антиоксидантный статус растений картофеля in vitro

In the present work researches on influence of chemically synthesized nanocomposites of selenium (NC) in natural polymeric matrices - arabinogalactan (selenium content 6.4%) and starch (selenium content 1.2%) on a condition of antioxidant system of potato plants are presented. It is known that during abiotic or biotic stress, lipid peroxidation products (POL) are formed in plant tissues and the content of reactive oxygen species (ROS) in plant tissues increases, which leads to dysfunction of the plant cell membrane, inhibition of biochemical processes in the plant cell and, as a consequence, cell death. Therefore, the main task of this work was the study of NC for antioxidant activity. Studies have been conducted on the effect of NC on the content of reactive oxygen species (ROS) in potato root tissues, the activity of peroxidase enzyme in the tissues of roots and leaves, and the content of lipid peroxidation products (LPO) - diene conjugates (DC) and malondialdehyde (MDA) in the tissues of roots and leaves of potatoes. Studies were conducted on uninfected potato plants of Lukyanovsky variety (susceptible variety) and infected with phytopathogenic bacterium Clavibacter sepedonicus (Cms) in vitro. The results of the study showed that NC affect the antioxidant system of plants during infection. NC action reduced the release of ROS, reduced the content of LPO products, and also reduced the activity of the enzyme peroxidase. Studies of the primary stress indicator – DC, it was found that only NC Se/Ag had a negligible effect when processing non-infected potato plants, but when at the same time, both studied substances reduced the indicator in the variant with infected plants. Thus, it was found that NC reduces the stress load on the infected plants, reducing cell damage by products of oxidative stress. Perhaps this effect is related to the nature of polysaccharide matrices and the size of encapsulated selenium nanoparticles in them and its toxic effect. Combining all the data obtained, we can talk about the effectiveness of NC in the fight against bacterial diseases of cultivated plants and assume its use as an effective and safe remedy against them.

Growth-Stimulating Activity of Natural Polymer-Based Nanocomposites of Selenium during the Germination of Cultivated Plant Seeds.

The growth-stimulating activity of three selenium nanocomposites (NCs) in various matrices based on arabinogalactan (NC Se/AG, 6.4% Se), starch (NC Se/St, 2% Se), and carrageenan (NC Se/Car, 12% Se) with respect to plants of radish, soybean, and potato was investigated. It was shown that the treatment of plant seeds with NCs stimulated root growth during germination. It was found that the studied NCs affected both the level of lipid peroxidation and the activity of the antioxidant enzyme glutathione peroxidase (GPX). The treatment of radish seeds with NCs stimulated root growth during their germination and reduced the content of diene conjugates (DC) in root tissues. It was shown that soaking seeds in NC Se/AG solution increased the GPX activity in the tissues of the radish root by 40%. Stimulation of soybean root growth under the influence of NC Se/Car may also be associated with the activation of GPX. Furthermore, in potato plants, this NC led to the stimulation of germination; however, this was probably due to the activation of other antioxidant enzymes. The results obtained allow us to consider Se NCs as potential plant growth stimulants.

ВЛИЯНИЕ НАНОСЕЛЕНА НА СОДЕРЖАНИЕ ИОНОВ K+, Na+ И Cl- В ПРОРОСТКАХ ПШЕНИЦЫ В УСЛОВИЯХ КОМБИНИРОВАННОГО ДЕЙСТВИЯ ЗАСОЛЕНИЯ И ЗАСУХИ

Soil salinization is one of the most important problems for agriculture. In most cases, the effect of salinization is manifested in conjunction with water deficiency, reinforcing each other. One of the most dangerous types of salinization is sodium chloride, in which both sodium and chlorine ions have a damaging effect on plants. The physiological processes taking place under stressful conditions with the participation of enzymes and antioxidants are closely related to each other. The content of some enzymes is significantly affected by selenium. The least toxic and bioavailable is selenium in the form of nanoparticles stabilized by natural biologically active substances. The aim of the work was to study the effect of the tread action of the original water-soluble composition of nanosalen stabilized with sodium alginate on the content of K+, Na+ and Cl— ions in wheat seedlings under the combined effects of salinization and drought. The objects of study were winter wheat seeds (Triticum aestivum L.) and a water-soluble composition of selenium nanoparticles. Wheat seeds were soaked for 4 hours in a solution of selenium nanocomposition at a concentration of 10.0; 20.0 and 30.0 mg/l. The control was seeds soaked in distilled water. Plants were grown for 10 days under controlled conditions in a climatic chamber in vessels with a capacity of 1.0 L., a 16-hour photoperiod, a temperature of 25/20 °C (day / night) and a relative humidity of 60±5 %. Well washed river sand was used as a substrate. On day 10, the mass of raw and dry matter and the content of K+, Na+ and Cl— ions in the leaves and roots, as well as on the water content of the leaf tissues and the relative water content (RWC) were determined. For the first time, a study was made of the protective effect of selenium nanocomposite on the content of K+, Na+ and Cl— in leaves and roots, as well as their relationship with the water status of leaves in wheat seedlings under the combined effect of chloride salinity and drought. The action of nanoselen increases the absorption of K+ ions by both roots and leaves. In this case, the absorption of Na+ ions are significantly reduced. The protective effect of nanoselen is also manifested in maintaining a high-water status.

Synthesis and antimicrobial activity of arabinogalactan-stabilized selenium nanoparticles from sodium bis(2-phenylethyl)diselenophosphinate

Water-soluble elemental selenium nanocomposites containing 0.8-3.9% Se were synthesized by oxidation of sodium bis(2-phenylethyl)diselenophosphinate in aqueous medium in the presence of the natural polysaccharide arabinogalactan as a stabilizer for nanoparticles. Using a complex of physicochemical research methods (TEM, XRD, IR spectroscopy), it was found that the nanocomposites were formed as spherical particles of amorphous selenium of 31–78 nm in size, which are dispersed in the polysaccharide matrix. For the nanocomposite containing 3.9% Se, a pronounced antimicrobial activity against the bacterial phytopathogen Clavibacter michiganensis subsp. sepedonicus was demonstrated.

Selenium nanocomposites with polisakharidny matrixes stimulate growth of in vitro potatoes infected with the ring rot disease

In article for the first time investigated influence of nanocomposites of selenium in matrixes of an arabinogalaktan (Se/Ag) and starch (Se/St) on vegetation of in vitro plants potatoes, peroxidase activity and reactive oxygen species. Earlier it was shown that these nanocomposites of selenium have antimicrobial effect to a phytopathogenic bacterium of Clavibacter michiganensis ssp. sepedonicus (Cms). In this real work it is shown that the Se/Ag (6,4% of Se) and Se/St (12% of Se) have no negative impact on the healthy and infected with Cms potatoes, stimulating its growth, quantity of leaves and mass of a vegetative part. NC Se/Ag has a positive effect on potato plants by increasing its immune status by increasing ROS content and increasing peroxidase activity. With application of the element analysis it is shown that the studied nanocomposites do not collect in potatoes fabrics after its processing by them. The Se/Ag and Se/St as potential agents for improvement of potatoes from pathogenic bacteria allow to consider the received results.

Selenium Nanocomposites Having Polysaccharid Matrices Stimulate Growth of Potato Plants in Vitro Infected with Ring Rot Pathogen.

The influence of nanocomposites (NC) of selenium in matrices of arabinogalactan (Se/AG) and starch (Se/St) on in vitro vegetation of potato plants, peroxidase activity, and reactive oxygen species has been thoroughly studied. It has been shown that these nanocomposites of selenium have antimicrobial effect to the phytopathogenic bacterium Clavibacter michiganensis ssp. sepedonicus (Cms). In the present investigation, it has been shown that Se/AG NC (6.4% of Se) and Se/St NC (12.0% of Se) have no negative impact on the potato plants healthy and Cms infected, while stimulating their growth, number of leaves and weight of the vegetative part. Se/AG NC has shown a positive effect on potato plants by increasing its immune status by increasing the ROS content and increasing the peroxidase activity. With the use of the element analysis technique, it has been shown that scrutinized nanocomposites are not accumulated in potato plants after the bactericidal processing with the nanocomposites. Se/AG NC and Se/St NC as potential agents used for treatment of potato plants against pathogenic bacteria.