The present study focuses on the development of a self-powered, label-free, and portable potentiometric sensor for precise detection of residual chlorpyrifos (CPY) in natural samples using copper oxide-infused chitosan-polypyrrole (CuO-in-CHIT-g-PPy) nanocomposite. The design and delicacy of the sensing setup have been described along with the structure, morphology, and physical properties of CuO-in-CHIT-g-PPy after using Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), and Scanning electron microscopy (SEM). The analytical result reveals the enhanced electrical conductivity, porosity, and responsiveness of the nanocomposite matrix for sensing applications. The proposed CPY sensor exhibits self-powered nature, a broader sensing range of i.e. 1.0–100.0 μM, high sensitivity of 2.44 mV µM−1 cm−2, Limit of Detection (LOD) of 0.75 μM, 50 s response time, 10 s recovery time and stability for 70 days at ambient condition. Additionally, the study also describes the sensor’s mechanism, based on the interaction between the electrode materials and CPY, to generate induced electrode potential with 99.7 % validity to the standard spectroscopy method.
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