Background/Objectives: The present paper investigated anti Candida effect using four types of fractions extracted from Rhizoma Coptidis. Methods/Statistical analysis: Disk diffusion tests were conducted to see the anti-Candida effect, and 70% ethanol and fluconazole (50mg/ml) were used as positive control group. This was to examine sensitivity on the sample by measuring a diameter of the inhibition zone cultured and created after placing a sample-treated disk. Findings: The test result of Hexane, Chloroform, n-butanol (n-BuOH), and ethyl acetate (EtOAc) showed that a diameter of n-BuOH was 32mm, which was 110% higher inhibition control than that of Fluconazole (50mg/ml) 29mm, which was a positive control group. Since existing antifungal agents have many side-effects, a dosage of existing drugs can be reduced through combined modality therapy using n-BuOH fraction or similar natural substances while similar or improved effect can be obtained. As discussed in the study result in this paper, the discovery of medicinal ingredient with antifungal effects as combined modality therapy can be regarded highly important. Improvements/Applications: In summary, the present study searched antifungal agents of new substance and identified the antifungal effect of n-BuOH fraction extracted from Rhizoma Coptidis on C. albicans infection.