Myoglobin Concentration Research Articles

Application of Lactiplantibacillus plantarum SCH1 for the Bioconservation of Cooked Sausage Made from Mechanically Separated Poultry Meat

The aim of the research was an assessment of the effect of the Lactiplantibacillus plantarum SCH1 strain isolated from ecological raw fermented pork roast on pH, redox potential, nitrites, and nitrates content, L a* b* color parameters, total heme pigments content, nitrosyl myoglobin concentration, as well as the microbiological quality and sensory traits of cooked sausages produced from mechanically separated poultry meat (MSPM), cured with a lower sodium nitrite level (NaNO2 50 mg/kg) after production as well as after storage (1 and 3 weeks of storage). The biochemical identification of the Lactobacillus bacteria after storage was also performed. Tests were performed in two sausage treatments: C—control sausage made from MSPM and L—sausage made from MSPM inoculated with L. plantarum at approx. 107 cfu/g. No negative effect of using the L. plantarum SCH1 strain on the physical and chemical MSPM sausage features was found. The treatment with L. plantarum SCH1 was of better microbiological quality after 3 weeks of storage. The sausages with L. plantarum SCH1 kept good sensory quality while the control treatment was spoiled after storage.

Rhabdomyolysis is Associated with In-Hospital Mortality in Patients with COVID-19.

Rhabdomyolysis (RM) has been associated with many viral infectious diseases, and associated with poor outcomes. We aim to evaluate the clinical features and outcomes of RM in patients with coronavirus disease 2019 (COVID-19). This was a single-center, retrospective, cohort study of 1,014 consecutive hospitalized patients with confirmed COVID-19 at the Huoshenshan Hospital in Wuhan, China, between February 17 and April 12, 2020. The overall incidence of RM was 2.2%. Compared with patients without RM, those with RM tended to have a higher risk of deterioration. Patients with RM also constituted a greater percentage of patients admitted to the intensive care unit (90.9% vs. 5.3%, P < 0.001) and a greater percentage of patients undergoing mechanical ventilation (86.4% vs. 2.7% P < 0.001). Moreover, patients with RM had laboratory test abnormalities, including the presence of markers of inflammation, activation of coagulation, and kidney injury. Patients with RM also had a higher risk of in-hospital death (P < 0.001). Cox's proportional hazard regression model analysis confirmed that RM indicators, including peak creatine kinase levels > 1,000 IU/L (HR = 6.46, 95% CI: 3.02-13.86) and peak serum myoglobin concentrations > 1,000 ng/mL (HR = 9.85, 95% CI: 5.04-19.28), were independent risk factors for in-hospital death. Additionally, patients with COVID-19 that developed RM tended to have delayed viral clearance. RM might be an important contributing factor to adverse outcomes in COVID-19 patients. The early detection and effective intervention of RM may help reduce mortality among COVID-19 patients.

Reduced Scattering Coefficient During Incremental Exercise Is Constant Without Being Affected by Changes in Muscle Oxygenation or Hemodynamics.

Previous studies have reported that the reduced scattering coefficient (μs') in the vastus lateralis changes during ramp-incremental exercise due to blood volume changes or accumulation of metabolic by-products. We aimed to clarify the influences of deoxygenation and blood volume changes during exercise on μs' dynamics in subjects with various aerobic capacities. Twenty-three healthy young men participated in this study. All subjects performed a ramp-incremental cycling exercise until exhaustion and were divided into two groups: lower (Low: n=12; peak pulmonary oxygen uptake per kg of fat-free mass (VO2peak), 54.2±5.3mL/kg/min) and higher aerobic capacity group (High: n=11; VO2peak, 69.7±5.2mL/kg/min) by median of VO2peak. Deoxygenated hemoglobin and myoglobin concentrations (deoxy[Hb+Mb]) and total [Hb+Mb] (total[Hb+Mb]) in the vastus lateralis were monitored during the exercise by three-wavelength (760, 800, and 830nm) time-resolved NIRS. Similarly, μs' at each wavelength was continuously monitored. With increasing exercise intensity, deoxy[Hb+Mb] and total[Hb+Mb] significantly increased in both groups, and the average values of the peak amplitudes of deoxy[Hb+Mb] and total[Hb+Mb] during exercise showed a 106.4% increase and a 17.9% increase from the start of the exercise, respectively. Furthermore, the peak amplitude of total[Hb+Mb] was significantly greater in High. Conversely, there were no changes in μs' at any wavelength during exercise and no differences between two groups, suggesting that the great deoxygenation and blood volume changes during incremental exercise have little effect on μs' dynamics.

Prolonged preoperative fasting induces postoperative insulin resistance by ER-stress mediated Glut4 down-regulation in skeletal muscles.

Preoperative fasting aims to prevent pulmonary aspiration and improve bowel preparation, but it may induce profound systemic catabolic responses that lead to protein breakdown and insulin-resistant hyperglycemia after operation. However, the molecular mechanisms of catabolic reaction induced by prolonged preoperative fasting and surgical stress are undetermined. In this study, anesthetized rats were randomly assigned to receive a sham operation or laparotomy cecectomy. Fasting groups were restricted from food and water for 12 h before operation, while the feeding group had free access to food throughout the study period. Twenty-four hours after operation, the animals were sacrificed to collect blood samples and soleus muscles for analysis. Postoperative blood glucose level was significantly increased in the fasting group with elevated serum insulin and C-peptide. Continuous feeding reduced serum myoglobin and lactate dehydrogenase concentrations. Preoperative fasting activated inositol-requiring transmembrane kinase/endoribonuclease (IRE)-1α and c-Jun N-terminal kinase (JNK) mediated endoplasmic reticulum (ER)-stress, and reduced glucose transporter type 4 (Glut4) expression in the soleus muscle. Phospholamban phosphorylation was reduced and intracellular calcium levels were increased in the isolated skeletal muscle cells. Similar results were found in ER stress-induced C1C12 myoblasts. The expression of Glut4 was suppressed in the stressed C1C12, but was potentiated following inhibition of ER stress and chelation of intracellular free calcium. This study provides evidence demonstrating that prolonged preoperative fasting induces ER stress and generates insulin resistance in the skeletal muscle through suppression of Glut4 and inactivation of Ca2+-ATPase, leading to intracellular calcium homeostasis disruption and peripheral insulin resistance.

366 The Ham Halo condition: Characterization and a possible approach for mitigation

Abstract The Halo condition is a meat quality defect characterized by very pale lean tissue on the superficial portion of fresh ham muscles. This tissue does not allow proper cured color development and has been the subject of customer complaints for ham processors. Surveys of raw materials revealed an issue affecting many suppliers and genetic lines. Further investigation indicated that the condition was most prominent in the distal portion of the biceps femoris muscle and was present in pigs of all ages and stages of production. Halo-affected tissue had much higher proportion of white muscle fibers. Consequently, Halo-affected tissue exhibited much lower myoglobin concentration, as well as much greater lightness, and much lower redness values, compared to normal tissue in the deeper portion of the muscle. These differences are consistent with increased expression of genes coding for white muscle fiber specific proteins in the Halo-affected tissue relative to tissue from the Inside portion of the biceps femoris muscle. Virtually all muscles evaluated have exhibited some degree of the Halo condition, but significant variation exists in the size of the affected portion of the muscle as well as the severity of the condition. Sire has a substantial impact on variation in Halo condition severity. Thus, genetic selection should help mitigate the condition. We will provide an overview of efforts to characterize the Halo condition and discuss genetic selection as a means to mitigate the condition.

Myoglobin clearance with continuous veno-venous hemodialysis using high cutoff dialyzer versus continuous veno-venous hemodiafiltration using high-flux dialyzer: a prospective randomized controlled trial

BackgroundMyoglobin clearance in acute kidney injury requiring renal replacement therapy is important because myoglobin has direct renal toxic effects. Clinical data comparing different modalities of renal replacement therapy addressing myoglobin clearance are limited. This study aimed to compare two renal replacement modalities regarding myoglobin clearance.MethodsIn this prospective, randomized, single-blinded, single-center trial, 70 critically ill patients requiring renal replacement therapy were randomized 1:1 into an intervention arm using continuous veno-venous hemodialysis with high cutoff dialyzer and a control arm using continuous veno-venous hemodiafiltration postdilution with high-flux dialyzer. Regional citrate anticoagulation was used in both groups to maintain the extracorporeal circuit. The concentrations of myoglobin, urea, creatinine, β2-microglobulin, interleukin-6 and albumin were measured before and after the dialyzer at 1 h, 6 h, 12 h, 24 h and 48 h after initiating continuous renal replacement therapy.ResultsThirty-three patients were allocated to the control arm (CVVHDF with high-flux dialyzer) and 35 patients to the intervention arm (CVVHD with high cutoff dialyzer). Myoglobin clearance, as a primary endpoint, was significantly better in the intervention arm than in the control arm throughout the whole study period. The clearance values for urea and creatinine were higher in the control arm. There was no measurable albumin clearance in both arms. The clearance data for β2-microglobulin and interleukin-6 were non-inferior in the intervention arm compared to those for the control arm. Dialyzer lifespan was 57.0 [38.0, 72.0] hours in the control arm and 70.0 [56.75, 72.0] hours in the intervention arm (p = 0.029).ConclusionsMyoglobin clearance using continuous veno-venous hemodialysis with high cutoff dialyzer and regional citrate anticoagulation is better than that with continuous veno-venous hemodiafiltration with regional citrate anticoagulation.Trial registrationGerman Clinical Trials Registry (DRKS00012407); date of registration 23/05/2017. https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00012407.

Relationships between Training Loads and Selected Blood Parameters in Professional Soccer Players during a 12-Day Sports Camp.

The main purpose of this study is to assess the relations between training loads and selected blood parameters in professional soccer players during a preseason sports camp. Fifteen professional soccer players (age: 24.3 ± 5.25 year; height: 182.6 ± 6.75 cm; weight: 76.4 ± 6.72 kg) participated in the 12-day training camp. All the training sessions and friendly games were accurately analyzed with a GPS system. Blood samples were taken from the players and analyzed before the camp (PRE), in the middle (MID), and one day after the camp (POST). Mean total distance covered by the players during the camp was 85,205 ± 2685 m, high-intensity running 12,454 ± 1873 m, and sprinting 639 ± 219 m. The highest aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and C-reactive protein (CRP) values were observed after six days of the camp. The application of intensive training during a 12-day sports camp can be associated with chronic muscle pain with high activity of some blood enzymes (CK, AST) and a high concentration of myoglobin (Mb). During training camps longer than 10 days, it would be necessary to apply, every second or third day, one day of rest, and the training load should not exceed two units every day.

Changes in Optical Path Length Reveal Significant Potential Errors of Muscle Oxygenation Evaluation during Exercise in Humans.

Near-infrared spectroscopy (NIRS), performed with a commonly available noninvasive tissue oxygenation monitoring device, is based on the modified Beer-Lambert law (MBLL). Although NIRS based on MBLL (NIRSMBLL) assumes that the optical path length (PL) is constant, the effects of changes in PL during exercise on muscle oxygenation calculated by MBLL are still incompletely understood. Thus, the purposes of this study were to examine the changes in optical properties during ramp incremental exercise and to compare muscle oxygen dynamics measured by time-resolved NIRS with those calculated based on MBLL. Twenty-two healthy young men performed ramp incremental cycling exercise until exhaustion. Optical properties (reduced scattering coefficient and PL) and absolute oxygenated, deoxygenated, and total hemoglobin and myoglobin concentrations (oxy[Hb + Mb], deoxy[Hb + Mb], and total[Hb + Mb], respectively) at the vastus lateralis were continuously monitored by a three-wavelength (763, 801, and 836) time-resolved NIRS device. The values of oxy-, deoxy-, and total[Hb + Mb] were then recalculated by assuming constant PL. PL at all wavelengths statistically significantly shortened during exercise. In particular, PL at 763 nm was greatly shortened, and the average changes during exercise were a 9.8% ± 3.1% reduction. In addition, significant differences in the kinetics of oxy-, deoxy-, and total[Hb + Mb] between directly measuring PL and assuming constant PL were found. The average changes in measured PL and assuming constant PL-deoxy[Hb + Mb] were increases of 28.8 ± 16.0 μM and increases of 16.4 ± 9.3 μM, respectively. Assuming constant PL in NIRSMBLL significantly underestimated actual muscle oxy/deoxygenation as compared with measurements obtained by real-time PL determination. The percent degree of the underestimated oxy/deoxygenation was greater than the percent degree of the changes in PL.

Sire Variation in the Severity of the Ham Halo Condition

A study was conducted to examine genetic variation in the ham halo condition. The distal portion of the biceps femoris was sampled by taking cores (2.54-cm diameter) from progeny (n = 1,016) from a Duroc meat quality–focused line. Commission Internationale de l ́Éclairage (CIE; “International Commission on Illumination”) color-space values (L*, a*, and b*) and myoglobin concentration were measured on the halo (“Halo”) and inside (“Inside”) portion of each core. The Halo portion of the biceps femoris had greater L* and b* and lesser a* and myoglobin content (all P &amp;lt; 0.001) than the Inside portion. Sires with 11 or more progeny were compared. The sire × muscle-location interaction affected (P &amp;lt; 0.001), L*, a*, and myoglobin concentration. Sire progeny groups differed for each trait in both portions of the muscle, but differences in the Halo portion of the muscle were not mirrored in the Inside portion of the muscle. Similarly, sire group affected the magnitude of the difference in L* (P = 1.4 × 10−4) and a* (P = 9.0 × 10−6) between the Halo and Inside portions of the muscle and tended (P = 0.08) to affect myoglobin content. However, the largest sire-group differences were not necessarily seen in the sires with the highest means for these attributes. Thus, selecting for myoglobin concentration, L*, or a* content in the Halo portion of the biceps femoris muscle would be an effective strategy for reducing the severity of the ham halo condition.

Effect of the exposure to oxidation and malondialdehyde on turkey and rabbit meat protein oxidative stability.

The present study aimed at evaluating the effect of the exposure to a strong oxidative environment (100mM NaClO) and the concurrent incubation with different malondialdehyde (MDA) concentrations (0 to 5mM) on protein carbonylation, free thiol groups, total heme pigments, and on the relative concentration of the different myoglobin (Mb) derivatives in turkey thigh and rabbit hind leg meat to elucidate their eventual role in inducing oxidative modifications on the protein fraction. With regard to turkey meat, the addition of a strong oxidant resulted in remarkably higher (P < 0.001) carbonyls along with a reduction in free thiol groups (which become undetectable). The relative concentration of MbO2 and MetMb was significantly affected (P < 0.001) and total heme pigment was reduced by 62% when fresh meat is compared to its oxidized counterpart. The addition of MDA 2.5mM (or greater) resulted in a 1.4-fold increase in carbonyls and a tendency (P = 0.07) has been observed for free thiol groups whose content decreased as the MDA level increased. Overall, the aforementioned results are in agreement with those obtained on rabbit hind leg meat in which a three-fold increase (P < 0.001) in carbonyls and a remarkably higher (P < 0.001) MetMb content coupled with a 53% reduction in free thiol groups were found. No significant differences ascribable to MDA addition were found. In conclusion, exposing turkey and rabbit meat to the same environment resulted in distinctive oxidative changes that might be related to the compositional traits and a species-specific susceptibility of the meat toward oxidation. PRACTICAL APPLICATION: Protein and lipid oxidation have been investigated in different meat types; nevertheless, the knowledge concerning the interaction between these two phenomena is limited. According to their compositional traits (high Polyunsaturated fatty acids (PUFA) and heme pigment content), turkey and rabbit leg meats are particularly susceptible to oxidation. Providing information concerning the oxidative stability of turkey thigh and rabbit hind leg meat exposed to strong oxidative conditions and to a concurrent increasing content of a secondary product of lipid oxidation, the findings of the present study can be useful when proper processing strategies and storage conditions have to be implemented for manufacturing processed products.

External Validity of the T-SAFT90: A Soccer Simulation Including Technical and Jumping Activities.

To examinethe physiological, muscle-damage, endocrine, and immune responses to a modified soccer-simulation protocol to include technical and jumping activities characteristic of match play (the Technical Soccer-Specific Aerobic Field Test; T-SAFT90). Eighteen university players (age23 [2]y, stature175 [5]cm, body mass74 [11]kg) performed the 90-minute protocol, with acute physiological responses monitored via heart rate, ratings of perceived exertion (6-20 scale), and body mass changes. Creatine kinase, myoglobin, cortisol, and leukocyte subset concentrations were measured at baseline, immediately (0h), and 24 hours post T-SAFT90. T-SAFT90 incurred an average heart rate equivalent to 87% (5%) of maximum, 16 (2)a.u. ratings of perceived exertion, and a 1.5% (1.0%) body mass deficit. Moderate to large proliferation of leukocyte subsets (P ≤ .01; leukocytes: 6.4-fold; neutrophils: 5.5-fold; lymphocytes: 2.0-fold) and increases in cortisol (2.3-fold) were observed at 0 hours (effect size = 1.13-3.52), each returning to baseline by 24 hours (P > .45; effect size = 0.05-0.50). Myoglobin peaked immediately post T-SAFT90 (4.8-fold), whereas creatine kinase (24h: 6.0-fold) showed a delayed time course (both P ≤ .001; very large effects; effect size = 2.66 and 3.43, respectively). The magnitude and time course of the physiological, immune, endocrine, and muscle-damage markers observed during and following T-SAFT90 are similar to values reported in match-play literature, demonstrating external validity of the simulation.

Clinical characteristics and outcome of 64 patients with severe COVID-19

Objective: To investigate the causes of death in patients with severe COVID-19. Methods: A retrospective analysis was performed on 64 patients with severe COVID-19 admitted to Wuhan Pulmonary Hospital from January 12, 2020 to February 28, 2020. There were 36 males and 28 females, aging from 44 to 85 years[median 68 (62, 72)]. Fifty-two patients (81%) had underlying comorbidities. The patients were divided into the death group (n=40) and the survival group (n=24) according to the treatment outcomes. In the death group, 24 were male, and 16 were female, aging from 49 to 85 years [median 69 (62, 72)], with 31 cases (78%) complicated with underlying diseases. In the survival group, there were 12 males and 12 females, aging from 44 to 82 years[median 66 (61,73)], with 21 cases (88%) with comorbidities. Clinical data of the two groups were collected and compared, including general information, laboratory examinations, imaging features and treatments. For normally distributed data, independent group t test was used; otherwise, Mann Whitney test was used to compare the variables. χ(2) test and Fisher exact test was used when analyzing categorical variables. Results: The median of creatine kinase isozyme (CK-MB) in the death group was 19.0 (17.0,23.0) U/L, which was higher than that in the survival group 16.5 (13.5,19.6) U/L. The median level of cTnI in the death group was 0.03 (0.03, 0.07) μg/L, which was significantly higher than that in the survival group (0.02, 0.03) μg/L, with a statistically significant difference between the two groups (P=0.007). The concentration of myoglobin in the death group was 79.5 (28.7, 189.0) μg/L, which was higher than 33.1 (25.7, 54.5) μg/L in the survival group. The level of D-dimer in the death group was 2.0 (0.6, 5.2) mg/L, which was higher than 0.7 (0.4, 2.0) mg/L in the survival group. The LDH level of the death group was 465.0 (337.5,606.5) U/L, which was higher than that of the survibal group, 341.0 (284.0,430.0) U/L, the difference being statistically significant (P=0.006). The concentration of alanine aminotransferase in the death group was 40.0 (30.0, 48.0) U/L, which was higher than 32.5 (24.0, 40.8) U/L in the survival group, and the difference was statistically significant (P=0.047).Abnormal ECG was found in 16 cases (62%) in the death group, which was significantly higher than that in the survival group (29%), the difference being statistically significant (P=0.024) .The main causes of death were severe pneumonia with acute respiratory distress syndrome (ARDS, n=20), acute heart failure(n=9), atrial fibrillation(n=3) and multiple organ dysfunction syndrome (MODS, n=3). Conclusions: ARDS caused by severe pneumonia and acute heart failure and atrial fibrillation caused by acute viral myocarditis were the main causes of death in severe COVID-19 patients. Early prevention of myocardial injury and treatment of acute viral myocarditis complicated with disease progression may provide insights into treatment and reduction of mortality in patients with severe COVID-19.

Cu-Doped ZnO Nanoparticles as an Electrochemical Sensing Electrode for Cardiac Biomarker Myoglobin Detection

Cu-doped ZnO nanoparticles were used in the form of a screen-printed electrode on a prefabricated gold-plated electrode for electrochemically determining the concentration of nanomolar myoglobin (Mb), a cardiac biomarker. Initially, Cu-doped ZnO nanoparticles were synthesised through the sol gel method by using three Cu-doping concentrations (varying number of Cu atoms). Then, the synthesised material was analysed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy, and field emission secondary electron microscopy. Analysis revealed a mixed phase of ZnO and CuO, whereas the vibration band in the range of 650-700 cm -1 indicated metal oxide formation. Morphological observation showed uniform-sized spherical particles of approximately 15-20 nm (as nanoparticles) that were evenly distributed, which were also confirmed based on the average particle size estimated using XRD data. The developed electrodes were tested for biomarker concentration from 3 to 15 nM based on cyclic voltammogram and impedance spectroscopic curves, where the redox potential/current/charge transfer resistance changed linearly with Mb and dopant (Cu) concentration. The sensitivity was estimated as 2.13-10.14~μAnM -1 cm -2 with dopant concentration of approximately 0.46 nM as the limit of detection.

Effect of adding pumpkin and flax oil to diets on the meats physical and chemical traits of broilers

This study was conducted in the fields of Animal Production Department/Faculty of Agriculture/Tikrit University during the period from 9th April, 2019 to 14th May, 2019 to know effect of adding pumpkin and flax oil to the diets on physical and chemical traits of the broiler meat. 480 day old chicks (Ross-308) were randomly distributed to eight treatments, each group was divided to three replicates(20 birds/replicate). T1 was a standard feed without supplementation, T2 was standard feed with 50 mg/kg feed of Neomycin antibiotic (positive control), while T3, T4 and T5 diets which sunflower Oil in the control diets replaced with pumpkin seed oil at concentrations 0.5, 1 and 1.5 %, respectively, otherwise T6, T7 and T8 diets replaced with flaxseed oil at concentrations 0.5, 1 and 1.5 %, respectively. The results showed significant decrease(P≤0.05) in T1, T3, T5, T6 and T8 compared to T2 in the drip loss but the treatments did not differ significantly in the thawing loss, all treatments decreased significantly in the loss during cooking and water holding capacity compared with T2. All treatments increased significantly(P≤0.05) in the myoglobin concentration compared with T1 except T4 and T7. The cholesterol concentration of meat decreased in all treatments compared to T1. T5 and T8 increased significantly compared with other treatments in pH of meat. All treatments decreased significantly (P≤0.05) except T4 and T5 compared to T2 in the percent of meat moisture. T6 increased significantly in protein percent compared with T2. All treatments decreased significantly compared with T2 in the fat percent. Ash percent in meat did not differ significantly among treatments. All oxidation indicators such as Thiobarbituric acid (TBA), Peroxide Value(PV) and Free Fatty Acids(FFA) increased significantly (P≤0.05) compared to T1 for meat stored in the freeze for one and half month. The percentage of all fatty acids increased significantly(P≤0.05) compared to T1 and T2.

Short-Term Resistance Training Supported by Whole-Body Cryostimulation Induced a Decrease in Myostatin Concentration and an Increase in Isokinetic Muscle Strength.

The study aimed to determine whether combining cryostimulation with resistance training would effectively increase muscle strength, and if so, whether this adaptation would be related to changes in circulating levels of exerkines (i.e., mediators of systemic adaptation to exercise). Twenty-five students completed 12 sessions of resistance training, each followed by either cryostimulation (n = 15, 3 min exposure at −110 °C) or passive recovery (n = 10). Prior to and post this intervention, participants performed two eccentric cycling bouts (before and after training). At these points, serum concentrations of muscle damage marker (myoglobin), exerkines (interleukin 6 (IL-6), interleukin 15 (IL-15), irisin, brain-derived neurotrophic factor), hypertrophy-related factors (myostatin, insulin-like growth factor 1), and muscle strength were measured. The applied procedure reduced the physiological burden of the second eccentric cycling bout and myoglobin concentrations only in the group subject to cryostimulation. The same group also exhibited decreased levels of myostatin (from 4.7 ± 1.7 to 3.8 ± 1.8 ng·mL−1, p < 0.05). A significant and large interaction between the group × time was noted in IL-15 concentration (p = 0.01, ). Training and cryostimulation induced a positive and likely significant improvement of isokinetic muscle strength. Altogether, obtained results support the claim that resistance training combined with cold exposure modified muscle strength through modulation of myostatin and IL-15 concentrations.

Characterization Of Normal Biomarkers Of Muscle Damage In Collegiate Athletes

Exertional rhabdomyolysis is a serious clinical condition in which skeletal muscle is rapidly broken down, potentially leading to life-threatening systemic complications. Clinicians often diagnose rhabdomyolysis based on elevations in circulating creatine phosphokinase (CPK) and symptomology. Normal CPK and other biomarker concentrations following intense exercise are unknown. PURPOSE: This study aimed to determine reference concentrations for selected biomarkers that suggest muscle damage in athletes and examine the time-course of muscle damage biomarker responses after strenuous exertion. METHODS: 20 collegiate NCAA I football players were enrolled in the study. Serum and urine samples were collected immediately and 24 hours post strenuous practice (0h and 24h, resp). Serum samples were analyzed for biomarkers of muscle damage including a Chem26 metabolic and chemistry panel and myoglobin. Urine samples were analyzed for creatinine and myoglobin concentrations. Participants were also given Physical Symptoms Questionnaires to obtain subjective measures of symptoms that may be related to severe muscle damage, or rhabdomyolysis. RESULTS: A total of 28 samples were collected with 9 repeats. CPK levels were elevated at 0h (958.2 ± 544.1 IU/L), but trended down 24h post practice (751.0 ± 410.5IU/L, p=0.059). LDH was acutely elevated at 0h (217.9 ± 30.6 IU/L) compared to the 24h timepoint (170.3 ± 35.5 IU/L, p<0.0001). Average serum myoglobin was higher post-practice (1.31 ± 1.57 ng/mL), but decreased 24 h post-exercise (0.36 ± 0.71 ng/mL, p<0.001). Similarly, urine creatinine concentrations were higher 0h post practice (390.1 ± 164.2 mg/mL), and decreased 24h post (214.2 ± 99.4 mg/dL, p<0.0001). Urine myoglobin was also elevated at 0h post practice (18.18 ± 36.7 ng/mL), but decreased 24h post (0.66 ± 1.43 ng/mL, p=0.001). The Physical Symptoms Questionnaires did not reveal any symptoms related to severe muscle damage. CONCLUSION: Biomarkers of muscle damage were elevated immediately post strenuous exercise, but dropped 24 hours post-practice. Serum and urine biomarkers of muscle damage collected immediately post strenuous exercise are unlikely to be specific markers of rhabdomyolysis, but rather of transient exercise-induced muscle damage in a highly trained athletic population.

P0635MYOGLOBIN CLEARANCE IN CVVHD USING HIGH CUT-OFF FILTER VERSUS CVVHDF WITH REGIONAL CITRAT ANTICOAGULATION: A PROSPECTIVE RANDOMIZED CONTROLLED TRIAL

Abstract Background and Aims Acute kidney injury (AKI) is frequent among critically ill patients and it is associated with high mortality. Rhabdomyolysis is a distinct cause of AKI. As myoglobin has renal toxic effects, clearance via continuous renal replacement therapy (CRRT) may be favourable. Current international guidelines recommend regional citrate anticoagulation (RCA). Clinical data comparing different modalities of CRRT addressing myoglobin clearance are lacking. Hemofiltration is recommended to eliminate myoglobin, which requires systemic anticoagulation. RCA would be impractical due to the high citrate load as a result of the higher blood flow. Continuous veno-venous hemodiafiltration (CVVHDF) is one opportunity to realize lower citrate load and maintain middle molecule clearance. Another approach may be the application of hemodialysis with high cut-off (HCO) membranes with a pore size larger than 0.01 μm in CVVHD (CVVHD-HCO). We have previously shown that CVVHD-HCO is superior to conventional high-flux dialyser regarding the clearance of β2-microglobulin. Based on this finding, we analyzed of various etiologiesre Filter lilifespan mit dem efonnummer der Virologie zum Befunderfragenmyoglobin clearance with CVVHDF compared to CVVHD-HCO with RCA. Method The present study is a prospective, randomized, single-blinded, monocentric trial. We enrolled patients from May 2017 to September 2018 in our 28-bed medical ICU at the University Hospital Leipzig. We screened 430 patients with AKI and indication for RRT for eligibility. A total of 70 patients underwent randomisation 1:1 into the intervention (CVVHD-HCO, high cut-off dialyzer Ultraflux EMiC2) and control group (CVVHDF, high-flux dialyzer Ultraflux AV1000S). The concentrations of myoglobin (17053 Dalton (Da), urea (60 Da), creatinine (113 Da), β2-microglobulin 11800 Da), interleukin 6 (IL-6, 26000 Da) and albumin (66470 Da) were measured before (pre) and after (post) the dialyzer 1, 6, 12, 24 and 48 h after initiating CRRT. We hypothesized non-inferiority of CVVHD-HCO versus CVVHDF regarding myoglobin clearance (primary end-point). Results Myoglobin clearance showed non-inferiority in the intervention group for all time points. The dialyzer in the intervention group resulted in even a better myoglobin clearance during the whole study period. (Table 1). Clearance values for urea and creatinine were equal and there was no albumin loss in both groups.β2-microglobulin clearance was significantly better in the intervention group than in the control group at all time points. The clearance of IL-6 was also better at T0 in the intervention group. The filter lifespan was 51.5 hours (CI: 44.24; 58.76) in the control group and 62.3 hours (CI 56.83; 67.7) in the intervention group (log-rank, p=0,029). Conclusion Myoglobin can be reliably cleared using both CVVHD-HCO and CVVHDF. Nevertheless, the plasma specific clearance rate of myoglobin was higher with CVVHD-HCO. High myoglobin levels are associated with AKI and higher mortality rates and on this account clearance of myoglobin represents a relevant clinical issue.

Effects of turmeric extract supplementation on inflammation and muscle damage after a half-marathon race: a randomized, double-blind, placebo-controlled trial.

Strenuous exercise induces inflammation and muscle damage. Turmeric (Curcuma longa L.) is a widely used spice that exhibits potent anti-inflammatory response and appears to decrease indirect markers of muscle damage. A randomized, double-blind, placebo-controlled trial was conducted to evaluate the effects of Curcuma longa L. extract (CLE) on inflammation and muscle damage after a half-marathon race. Twenty-eight healthy, normal-weight men were randomly assigned to one of two groups: (1) CLE (3 capsules per day, 500mg each); or (2) placebo (PLA, 3 capsules per day, 500mg of microcrystalline cellulose). Participants received the intervention for 4weeks and immediately before and after the half-marathon race. Creatine kinase, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, myoglobin, interleukins 6 and 10 were assessed at baseline, immediately before, after, and at 2, 24, and 48h after the half-marathon race. The half-marathon race increased markers of inflammation and muscle damage. A greater increase in interleukin-10 was observed in the CLE group immediately after the competition compared to the PLA group(7.54 ± 1.45 vs 5.25 ± 0.59pg/mL; p < 0.05; d = 0.55). Myoglobin concentration was lower 2h after the race in participants from the CLE group compared to the PLA group(62.10 ± 8.26 vs 107.85 ± 18.45ng/mL; p = 0.01; d = 0.86). Curcuma longa L. extract supplementation leads to an increase in IL-10 and decreased myoglobin in recreational male runners after a half-marathon race. U1111-1179-6335, February 13, 2016.

Polygenic Profile and Exercise-Induced Muscle Damage by a Competitive Half-Ironman.

Del Coso, J, Salinero, JJ, Lara, B, Gallo-Salazar, C, Areces, F, Herrero, D, and Puente, C. Polygenic profile and exercise-induced muscle damage by a competitive half-ironman. J Strength Cond Res 34(5): 1400-1408, 2020-To date, it is still unknown why some individuals develop higher levels of muscle damage than other individuals, despite participating in exercise with comparable levels of physical intensity. The aim of this investigation was to analyze 7 single-nucleotide polymorphisms (SNPs) that are candidates to explain individual variations in the level of muscle damage attained during a half-ironman competition. Using the model of Williams and Folland (2, 1, and 0 points for optimal, intermediate, and suboptimal genotype), we determined the total genotype score from the accumulated combination of 7 SNPs (ACE = 287bp Ins/Del; ACTN3 = p.R577X; creatine kinase, muscle type = NcoI; insulin-like growth factor 2 = C13790G; interleukin-6 = 174G>C; myosin light chain kinase = C37885A; and tumor necrosis factor-α = 308G>A) in 22 experienced triathletes. Before and after the race, a sample of venous blood was obtained to measure serum markers of muscle damage. Two groups of triathletes were established according to their postcompetition serum CK concentration: low CK responders (n = 10; 377 ± 86 U·L) vs. high CK responders (n = 12; 709 ± 136 U·L). At the end of the race, low CK responders had lower serum myoglobin concentrations (384 ± 243 vs. 597 ± 293 ng·ml, p = 0.04). Although the groups were similar in age, anthropometric characteristics, and training habits, total genotype score was higher in low CK responders than in high CK responders (7.7 ± 1.1 vs. 5.5 ± 1.1 point, p < 0.01). A favorable polygenic profile can contribute to reducing the level of muscle damage developed during endurance exercise.

The Relationship Between muscle Damage and Activity Profiles During Team Handball Matches

Background: Anaerobic activities often require explosive muscle power; it is therefore possible that players’ skeletal muscles sustain damage during the game, which leads to a performance decrease as the game progresses. Objective: This study investigated the relationship between muscle damage and activity profiles during team handball matches. Methods: This study conducted two handball games to examine the relationship between muscle damage and impacts against the body during the games. We studied one handball match between members of the same university team (Game I: 12 male court players) and a practice match between a Japanese handball league team and the university student team (Game II: nine male court players and six controls). Results: Plasma myoglobin concentration and plasma creatine kinase activity, both of which are biomarkers for muscle damage, increased to above their normal ranges after both games. The magnitudes of the changes in both plasma myoglobin (p&lt;.05) and plasma creatine kinase activity (p&lt;0.05) from before to after the game were significantly different between the players and controls in Game II. There were significant correlations between the number of shots taken in Game II and biomarkers for muscle damage; the changes in plasma myoglobin concentrations (p&lt;0.01) and plasma creatine kinase (p&lt; 0.01) activity levels. Conclusion: These results suggest that team handball matches involve high-intensity exercise that is sufficient to cause muscle damage. Additionally, our findings suggest that the severity of muscle damage is related to the specific actions associated with taking shots, such as jumping and colliding with a defender.