White oyster mushrooms is one of the commercial edible mushrooms, high and prospective economy value as a source of the income of farmers. This fungus can also grow on media that is given the addition of sucrose (sand sugar). Sucrose have the ability in increase of conidia germination and fungal growth. The purpose of this study was to determine the effect of adding the right dose of sucrose sugar to the growth of white oyster mushroom mycelium. This study used the experiment method. The results obtained from this study were effective doses of sugar in the long growth of oyster mushroom mycelium in F2 seedlings were 40 grams from 4% of growing media with an average growth of 0.67 cm per 2 days, while in seed F3 were 40 grams from 4% of growing media with an average growth of 0.36 cm per 2 days.