We demonstrate a femtosecond all-polarization-maintaining Nd fiber laser working at 920 nm mode locked by a biased non-linear loop mirror. The broadest spectral width of the pulse is 25.2 nm and the output power is 8 mW with 320 mW pump power. The measured pulse width is 109 fs with extra-cavity compression. The laser configuration of all-polarization-maintaining fiber can directly enhance the environmental stability of generated pulses. The seed pulses of the oscillator were amplified over 400 mW, which served as the light source for a two-photon microscope. To the best of our knowledge, this is the first demonstration of a 920 nm femtosecond Nd polarization-maintaining fiber laser based on a non-linear loop mirror.