Murraya Koenigii Leaf Extract Research Articles

Screening of Antimicrobial Activity of Murraya koenigii Leaf Extracts Against Pathogenic Bacterial Strains Staphylococcus aureus and Escherichia coli Isolated from Contaminated Water

Aim: Murraya koenigii is a widely used plant both as a potential medicinal agent and also for common cooking purposes. Aim of this present study was to determine the antimicrobial activity of Murraya koenigii leaf extracts on Staphylococcus aureus and Escherichia coli. 
 Study Design: Screening and isolation of pathogenic bacterial strains from contaminated water. Preparation of Murraya koenigii leaf extracts using petroleum ether, acetone and ethyl acetate by using serial extraction method with Soxhlet apparatus.
 Place and Duration of Study: Department of microbiology, Agro biotec research centre Ltd, Poovanthuruthu, Kottayam, Kerala, India, between 2014 January to 2014 May.
 Methodology: Staphylococcus aureus and Escherichia coli were the bacterial strains used in this study. Morphological and biochemical analysis of microorganisms were conducted to identify the strains. Leaf extracts (petroleum ether, acetone and ethyl acetate) of Murraya koenigii were screened using MHA disc diffusion methods.
 Results: Various concentration of plant extracts were used to check its activity against isolated pathogens. Acetone extract of curry leaves exhibit maximum zone of inhibition against Staphylococcus aureus and petroleum ether extracts shown maximum inhibition against Escherichia coli.

Biogenic synthesis, characterization and photocatalytic activity of cerium nanoparticles for treatment of dyes contaminated water

In this study, Murraya Koenigii leaf extract mediated cerium oxide nanoparticles (Ce-NPs) were synthesized using an eco-friendly approach. The surface morphology, elemental composition, surface functional groups, crystal structure and thermal stability of the synthesized Ce-NPs were examined using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-Ray diffraction (XRD) and thermogravimetric (TGA) analysis. The synthesized Ce-NPs were evaluated for their photocatalytic activity with respect to degradation of methylene blue dye (MBD) and acid orange-7 dye (AO7D) aqueous solution in the presence of sunlight. The dye degradation activity of the developed Ce-NPs was analyzed by the analysis of absorbance in treated and untreated aqueous dyes solution using UV–Vis spectrophotometer. The result of the study inferred that Ce-NPs have promising photocatalytic degradation ability (>98 %) for both AO7D and MBD (concentration = 10 mg L−1 of each dye) within 100 min exposure to sunlight.

Green Synthesis of Silver Nanoparticles using Coriandrum sativum and Murraya koenigii Leaf Extract and its Thrombolytic Activity

Background: Plants have been used for ages in traditional medicine, and it is exciting to perceive how recent research has recognized the bioactive compounds liable for their beneficial effects. Green synthesis of metal nanoparticles is a hastily emergent research area in nanotechnology. This study describes the synthesis of silver nanoparticles (AgNPs) using Coriandrum sativum and Murraya koenigii leaf extract and its thrombolytic activity. Objective: The aim of the study was to determine the clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles. Method: Leaves of Coriandrum sativum and Murraya koenigii were collected. Methanolic extraction of the plant sample was done through a Soxhlet extractor. The methanolic extract obtained from both the leaves was subjected to GC-MS analysis. The synthesized NPs from leaf extracts were monitored for analysis, where the typical X-ray diffraction pattern and its diffraction peaks were identified. 3D image of the NPs was analysed by Atomic Force Microscopy. The surface charge of nanoparticles was identified by Zeta potential. The Clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles were analysed by the modified Holmstorm method. Result: The thrombolytic property of the methanolic extract of plants Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 45.99% activity, and Murraya koenigii extract with 66.56% activity. The nanoparticles (Nps) from Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 58.29% activity, and NPs from Murraya koenigii with 54.04 % activity. Coriandrum sativum in GC-MS exhibited 3 peaks, whereas Murraya koenigii extract showed five peaks with notable bioactive compounds. conclusion: These NPs were further used for biomedical applications after being fixed by an organic encapsulation agent. The present research reveals the usefulness of Coriandrum sativum and Murraya koenigii for the environmentally friendly manufacture of silver nanoparticles.

Evaluation of Synergistic Hypolipidemic Activity of Murraya koenigii and Atorvastatin suspension in Hyperlipidemic rats

The purpose of this study is to assess the hypolipidemic potential of formulated suspensions of atorvastatin, alcoholic extract of Murraya koenigii and their combination on hyperlipidemic Wistar rats. Hyperlipidemia was induced in all groups except negative control group, using High Fat Diet (HFD). Formulated suspensions of Murayya koenigii leaf extract, atorvastatin and its combination were administered to HFD fed rats for 28 days. Treatment related changes in body weights were recorded. Extraction of Murraya koenigii was carried out by maceration. After completion of treatments, blood samples were collected immediately and serum separated was used for the estimation of lipid profile and glucose levels. Treatment with all the formulated suspensions shows significant normalization of body weight compared with positive control group (62.5±3.14) in HFD fed animals. The effect of combined suspension was more pronounced (40.08±4.86) and shows significant reduction in body weight gain, P<0.01 than that of Murraya koenigii leaf extract (57.94 ±4.36) or atorvastatin suspensions (45.97± 4.48) alone. Combined suspension also exhibits significant reduction, P<0.001 in serum TC, TG, LDL and VLDL. It also enhanced the cardio protective lipid HDL after 28 days of treatment. A significant reduction in serum glucose levels were noted with suspension of Murraya koenigii leaf extract alone, P<0.05 compared with positive control. It is possible to draw the conclusion from the current study that combining atorvastatin and Murraya koenigii leaf extract in a suspension may have had a synergistic effect on lowering body weight and hypolipidemic activity.

Green synthesis of silver nanoparticles using Murraya koenigii leaf extract with efficient catalytic, antimicrobial, and sensing properties towards heavy metal ions

Green synthesis of silver nanoparticles using Murraya koenigii leaf extract with efficient catalytic, antimicrobial, and sensing properties towards heavy metal ions

Green synthesis and characterization of mixed-phase Fe2O3 nanorods as a novel magnetically recoverable heterogeneous catalyst for Biginelli synthesis

For the first time, mixed-phase (Hematite and Maghemite) magnetic Fe2O3 nanorods were successfully biosynthesis by sol-gel auto-combustion method using the 1:1 mixture of Eucalyptus citriodora and Murraya koenigii leaf extract as a capping agent, and its catalytic effect on synthesis of 6-(chloromethyl)-1,2,3,4-tetrahydro-2-pyrimidinone (THPMs) derivatives were investigated. Further, the phase formation, surface topography, and crystallinity of biosynthesized Fe2O3 nanorods (NRs) were explored using powder XRD (X-Ray Diffraction), UVDRS (UV–Visible Reflectance Spectroscopy), FTIR (Fourier Transform Infrared Spectroscopy), FESEM (Field Emission Scanning Electron Microscopy), EDX (Energy Dispersive X-Ray), and VSM (Vibrating Sample Magnetometry). Furthermore, the catalytic activity of biosynthesized Fe2O3 NRs (C1, C2, and C3) was examined for one-pot synthesis of ethyl 6-(chloromethyl)-1,2,3,4-tetrahydro-2-oxo-4-arylpyrimidine-5-carboxylate via Biginelli reaction. To achieve high yields (93–99 %) of 6-(chloromethyl)-1,2,3,4-tetrahydro-2-pyrimidinone derivatives, this heterogeneous catalytic method is used with a wide range of aromatic aldehydes within a minimum reaction time, simple reaction work-up, and easily recoverable catalyst by an external magnet. The recovered catalyst is then employed for five successive cycles without non-noticeable loss of catalytic activity. We believe that this protocol presents a broad scope for Biginelli reaction through greenly produced and magnetically separable heterogeneous catalysts.

Photocatalytic Organic Contaminant Degradation of Green Synthesized ZrO2 NPs and Their Antibacterial Activities

The green synthesis of metal oxide nanoparticles is an efficient, simple, and chemical-free method of producing nanoparticles. The present work reports the synthesis of Murraya koenigii-mediated ZrO2 nanoparticles (ZrO2 NPs) and their applications as a photocatalyst and antibacterial agent. Capping and stabilization of metal oxide nanoparticles were achieved by using Murraya koenigii leaf extract. The optical, structural, and morphological valance of the ZrO2 NPs were characterized using UV-DRS, FTIR, XRD, and FESEM with EDX, TEM, and XPS. An XRD analysis determined that ZrO2 NPs have a monoclinic structure and a crystallite size of 24 nm. TEM and FESEM morphological images confirm the spherical nature of ZrO2 NPs, and their distributions on surfaces show lower agglomerations. ZrO2 NPs showed high optical absorbance in the UV region and a wide bandgap indicating surface oxygen vacancies and charge carriers. The presence of Zr and O elements and their O=Zr=O bonds was categorized using EDX and FTIR spectroscopy. The plant molecules’ interface, bonding, binding energy, and their existence on the surface of ZrO2 NPs were established from XPS analysis. The photocatalytic degradation of methylene blue using ZrO2 NPs was examined under visible light irradiation. The 94% degradation of toxic MB dye was achieved within 20 min. The antibacterial inhibition of ZrO2 NPs was tested against S. aureus and E. coli pathogens. Applications of bio-synthesized ZrO2 NPs including organic substance removal, pathogenic inhibitor development, catalysis, optical, and biomedical development were explored.

Synergism effect of Murraya koneigii and Piper betle leaves extracts on antibacterial activity against Gram Positive bacteria

Antimicrobial resistance has become the major threat to the health worldwide. It has lead to the ineffective impact of the ability of the substance or antibiotics to kill the bacteria. Therefore,there is a need to find a new alternative to overcome the problem by using natural products. In this study Piper betle (P. betle) and Murraya koenigii (M. koenigii) leaf extracts were used to measure their synergism effect towards antimicrobial treatments. Previous study has shown that both plants has shown their ability in inhibiting the growth of bacteria separately. Three different concentration ratios of the synergised leaf extracts were used with 1:1(P.betle:M. koenigii) ratios, 2:1 (P.betle:M. koenigii) ratios and 1:2 (P.betle:M.koenigii) ratios.The synergised leaf extracts was at the highest inhibition zone with combination of 2:1 ratio of P.betle and M. koenigii with 22.6mm. However, it does not have the greater inhibition ability as compared to the P.betle leaf extract with 28.1mm . Therefore, from the study, antagonism effect was discovered when both plant extracts were combined.

Murraya koenigii mediated synthesis of cobalt doped NiO nanoparticles: Evaluation of structural, optical properties and anti-bacterial activity

Recently, the synthesis of nanoparticles using phytoextracts has been focused worldwide due to their reduced chemical toxicity. We prepared NiO and Co-doped NiO nanoparticles using Murraya Koenigii leaf extracts to replace synthetic bactericides. The prepared samples were characterized by XRD, UV–Vis, FTIR, SEM, and EDAX analytical techniques. According to XRD analysis, the green synthesised NiO and Co-doped NiO nanoparticles have a crystalline cubic structure. NiO and Co-doped NiO nanoparticles showed a characteristic absorption peak at 348 nm. FTIR spectroscopy confirmed the functional groups of biomolecules present in the synthesised materials. The size of the prepared nanoparticles was found to be 80–250 nm as observed through SEM analysis. Enhanced antibacterial activity was observed for Co doped NiO against gram negative (Klebsiella pneumonia, Shigella dysenteriae ), gram positive ( Staphylococcus aureus , and Bacillus) bacterial strains. • Murraya Koenigii leaf extracts assisted green synthesis was adopted to prepare NiO and Co doped NiO nanoparticles. • The effect of Co doping on structural, optical and morphological properties of NiO nanoparticles were analysed. • The excellent antimicrobial activity of pure and doped NiO nanoparticles were reported.

Efficacy of some indigenous products against cowpea aphid, Aphis craccivora Koch

An experiment was conducted in field during 2017-18 for efficiency evaluation of seven indigenous products along with a check (imidacloprid 17.8 SL at 20g a.i /ha) to manage Aphis craccivora Koch on cowpea. In the bio-efficacy study, leaf extract of Polygonum hydropiper at 5% was found to be most effective and efficient in aphid population reduction (up to 77.48%), followed by neem oil at 1% (74.11%), Ocimum sanctum leaf extract at 5% (74.00%), Murraya koenigii leaf extract at 5% (70.96%), ash at 10 kg/ha (68.04%), river sand + neem leaf powder at 10 kg/ha (3:1 ratio) (44.48%) and ash + river sand at 10 kg/ha (5:1 ratio) (42.53%) after 7 days of spraying.

Catalytic degradation of Methylene blue dye and Antibacterial study of silver nanoparticles obtained using Murraya Koenigii leaf Extract

Introduction: The development of eco-friendly, cost-effective green protocol for the synthesis of nanomaterials is a significant feature of nanobiotechnology connecting synthesis of nanomaterials using microorganisms, including bacteria, viruses, fungi as well as plant and animal based products (1). There is much room of more development in the synthesis of nanoparticles using different biological resources as the source of reducing and stabilizing agents and their potential applications. Silver nanoparticles (AgNPs) are of great interests owing to their optical, electrical, catalytic, and antibacterial properties (1). For waste-water treatment, nanoparticles can exhibit effectiveness in catalytic dye degradation due to the characteristic catalytic properties of nanoparticles (2). In this paper, we have demonstrated an eco-friendly green approach for the room temperature synthesis of biocompatible AgNPs by the reduction of aqueous silver ions using Murraya koenigii (Mk) leaf extract. Methods: The aqueous plant extract was prepared by taking 10 g of pulverized Mk leaves in a 500 mL Erlenmeyer flasks with 60 mL of Milli-Q water and refluxed it for 15 min with constant stirring using magnetic stirrer (2). 9 mL (0.625%) NH3 solution was added to 2 L of 10-3 M aqueous AgNO3 solution followed by the addition of 90 mL of aqueous Mk leaf extract at controlled pH = 7 and homogenized by using a magnetic stirrer. Then the reaction mixture was kept for 5 h at room temperature followed by centrifugation at 6000 rpm for 30 min. The envisaged color change from transparent yellow to dark brownish indicates the formation of AgNPs (2). Results & Discussions: Synthesized AgNPs were well characterized by UV-Visible absorption spectroscopy, FTIR spectroscopy, XRD, DLS, HRTEM and EDX studies. AgNPs would exhibit SPR band at 402 nm. FTIR studies confirmed the presence of different biomolecules responsible for the stabilization as well as formation of AgNPs. Surface morphologies and size distribution of synthesized AgNPs were determined by HRTEM and DLS techniques, respectively. The average particle size was found 20 nm. XRD analysis confirmed the formation of face-centered cubic (FCC) crystalline lattice of AgNPs. EDX studies confirmed the presence of elemental silver (Ag). AgNPs can act as photocatalyst for the degradation of methylene blue (MB) used as a model cationic dye. AgNPs were nontoxic in nature as concluded from cytotoxicity studies and also exhibited substantial antibacterial activities towards gram-positive and gram-negative bacteria. Conclusions: In the present study, stable AgNPs were successfully prepared by a green route using aqueous Murraya koenigii leaf extract in simple, cost-effective, eco-friendly way using less hazardous reagents and solvents, and thereby non-toxic towards both human health and the environment. AgNPs were almost spherical in shape, crystalline in nature, with an average diameter of 20 nm. AgNPs showed catalytic activity for the degradation of a cationic dye MB. AgNPs were nontoxic in nature and can exhibit considerable antibacterial activity towards both gram-positive and gram-negative bacteria. Keywords: Biosynthesis, AgNPs, photocatalysis, methylene blue, antibacterial activity Acknowledgment: UKS would like to acknowledge financial support from the projects funded by the DHESTBT (Department of Higher Education, Science, Technology and Biotechnology), Government of West Bengal (Memo no. 161(sanc)/ST/P/S&T/9G-50/2017.

Evaluation of Toxicity and Antiangiogenic Activity of Murraya koenigii Leaf Extracts in Zebrafish

Abstract Introduction This study aimed to evaluate the toxicity and the antiangiogenic activity of Murraya koenigii leaf extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of M. koenigii leaves were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antiangiogenic activity of the M. koenigii leaf extracts was evaluated in a transgenic zebrafish (Tg [fli1: GFP]) that express GFP under the control of fli1, a transcription factor involved in vasculogenesis. Results The LC50 value of the aqueous extract of M. koenigii leaves was much larger (2,450 ppm) compared with that of ethanol extract (9.85 ppm), suggesting a lower toxicity of the aqueous extract. The antiangiogenic activity was evident only in aqueous extract, with significant effects in formation of intersegmental vessels and subintestinal veins in treated Tg (fli1: GFP) zebrafish embryos. Conclusion This study demonstrated the biological activity of M. koenigii leaf extracts as angiogenesis suppressor, suggesting its therapeutic potential as an angiogenesis inhibitor. Identification of active components from natural sources with potent antiangiogenic activity could lead to the development of anticancer agents.

Murraya koenigii Derived Phytochemicals against Dysentery

Phytochemicals are any of various biologically active compounds found in plants. These are produced by plants and have biological activity. It has been reported that Murraya koenigii leaf extract is traditionally used to cure dysentery. Dysentery is an intestinal inflammation, primarily of the colon. It can lead to mild or severe stomach cramps and severe diarrhoea with mucus or blood in the faeces. It is the infection in the intestinal tract. One enzyme, which is involved in its biochemical pathway, is known as alcohol dehydrogenase (which have pdb id.1Y9A). The molecular docking was studied by biovia discovery studio. In which the interaction is done between the phytochemical of the plant with the enzyme. The stability of the interaction was evaluated based on –CDocker energy and –CDocker interaction energy.

Green synthesis of calcium oxide nanoparticles at different calcination temperatures

PurposeThe purpose of this paper is to prepare calcium oxide (CaO) nanoparticles by green synthesis technique and study its structural, morphological and optical properties. The prepared samples were calcined at, 200°C, 300°C and 500°C, and the variation in the properties at different temperatures were investigated.Design/methodology/approachGreen approach has been used in the present work to synthesise the CaO nanoparticles using Murraya Koenigii leaf (curry leaves) extract as a capping agent. This technique involves the use of nontoxic reagents and natural products derived from various parts of plants.FindingsStudies reveal that CaO nanoparticles with good optical properties can be synthesized successfully by green approach. The optical absorbance spectra show a broad absorption peak around 400–500 nm. FTIR studies confirm the presence of different functional groups that help in the stabilization of CaO nanoparticles. PL emission spectra show a high intensity emission peak at around 231 nm in addition to peaks at 375 nm and 400 nm. XRD studies show planes of CaO cubic phase. SEM images show a nonuniform distribution of spherical particles along with some clusters. EDX spectra confirm the presence of calcium and oxygen.Practical implicationsCaO nanoparticles have wide applications in optical devices as well as in phototherapy. It is thus interesting to devise new and eco-friendly techniques to synthesise these nanoparticles to suit these applications.Originality/valueThis work would provide a new insight into the preparation of different metal oxide nanoparticles by a very simple and cost-effective green technique without the use of toxic reagents.

Phytoreduced copper oxide nanoparticles by using Murraya koenigii leaf extract and its antibacterial activity

The present study reported an eco-friendly, cost-effective and simple method for the synthesis of phytoreduced copper oxide nanoparticles using Murraya koenigii leaf extract. The synthesized CuO NPs wear confirmed by the electronic spectroscopy and morphological verification by XRD, FT-IR, EDS, UV–Vis, SEM, and TEM. The surface plasmon resonance peak at 320 nm in UV–VIS spectrum which favours phytochemically reduction of CuO NPs. While results of TEM observed average particles size 40–50 nm. Furthermore, antibacterial activity of synthesized CuO NPs was tested against E.coli, K. pneumoniae, S. aureus, and E. faecalis. These results showed effective antibacterial activity against K. pneumoniae and E. faecalis.

Fabrication and characterization of silver nanostructures using spherical silver nanoparticles released from Murraya koenigii leaf extract

Fabrication and characterization of silver nanostructures using spherical silver nanoparticles released from Murraya koenigii leaf extract

Immobilization of xylose reductase enzyme on cysteine-functionalized Murraya koenigii mediated magnetite nanoparticles

Murraya koenigii leaf extract mediated cysteine-functionalized magnetite nanoparticles (Cys-FeO NPs) were synthesized by the precipitation method. Cys-FeO NPs were activated using glutaraldehyde cross-linker (Glu-Cys-FeO NPs), and were used to immobilize xylose reductase (XR-FeO NPs). The effects of immobilization process parameters such as concentration of NPs, enzyme and cross-linking time were examined. The physicochemical properties of free XR, NPs and XR-FeO NPs were investigated. Results showed that Cys-FeO NPs have cubic spinel phase structures with spherical shape and sizes ranging from 90 to 100 nm. The successful binding of XR on functionalized NPs was confirmed through functional and structural studies. Minor structural rearrangements were observed in XR-FeO NPs compared to free XR enzyme. Under optimized conditions, the residual activity of Free XR and immobilized enzyme was found to be 80 and 85% respectively. Kinetic constant (Km) of free XR depicted that the immobilized enzyme had an increased binding affinity for the substrate.

Polyphenols-Induced Apoptosis by Murraya Koenigii against Lymphatic Filarial Parasite and Its Therapeutic Implication

ABSTRACTThe present study was designed to explore and validate the antifilarial activity of Murraya koenigii leaf extracts against Brugia malayi parasite. Anti-microfilarial activity of M. koenigii leaves extracted in methanol and ethanol (along with polyphenols depleted fractions) was determined. Ethanolic extract showed higher polyphenols content and anti-microfilarial activity compared to methanolic extract. Polyphenols depletion reduced such activity of ethanolic but not of methanolic extract. Ethanolic extract had lower alkaloids than methanolic extract. Ethanolic extract of M. koenigii leaves caused oxidative stress with concomitant apoptosis along with mitochondrial damage.Abbreviations: DEC: Diethyl carbamazine; DNPH: 2, 4 dinitro phenyl hydrazine reagent; DTNB: 5,5-dithio-bis-(2-nitrobenzoic acid); EB/AO: Ethidium bromide/Acridine orange; MDA: Mass Drug Administration; Mf: Microfilariae; PBS: Phosphate buffered saline; TCA: Trichloroacetic acid; WHO: World Health Organization

Growth-disrupting Murraya koenigii leaf extracts on Anopheles gambiae larvae and identification of associated candidate bioactive constituents

Plant-based constituents have been proposed as eco-friendly alternatives to synthetic insecticides for control of mosquito vectors of malaria. In this study, we first screened the effects of methanolic leaf extracts of curry tree (Murraya koenigii) growing in tropical (Mombasa, Malindi) and semi-arid (Kibwezi, and Makindu) ecological zones of Kenya on third instar An. gambiae s.s. larvae. Extracts of the plant from the semi-arid region, and particularly from Kibwezi, led to high mortality of the larvae. Bioassay-guided fractionation of the methanolic extract of the leaves of the plants from Kibwezi was then undertaken and the most active fraction (20 fold more potent than the crude extract) was then analyzed by Liquid chromatography quadruple time of flight coupled with mass spectrometry (LC-QtoF-MS) and a number of constituents were identified, including a major alkaloid constituent, Neplanocin A (5). Exposure of the third instar larvae to a sub-lethal dose (4.43 ppm) of this fraction over 7-day periods induced gross morphogenetic abnormalities in the larvae, with reduced locomotion, and delayed pupation. Moreover, the few adults that emerged from some pupae failed to fly from the water surface, unlike in the untreated control group. These results demonstrate subtle growth-disrupting effects of the phytochemical blend from M. koenigii leaves on aquatic stages An. gambiae mosquito. The study lays down some useful groundwork for the downstream development of phytochemical blends that can be evaluated for integration into eco-friendly control of An. gambiae vector population targeting the often overlooked but important immature stages of the malaria vector.

Green Synthesis of Silver Nanoparticles Using Murraya koenigii Leaf Extract and Their Antibacterial Potential

The eco-friendly and green synthesis of silver nanoparticles (AgNPs) was done by using the extract of young leaves of medicinally important plant Murraya koenigii (Curry Leaves). This method was very simple and rapid multiplication. The green synthesized silver nanoparticles were characterized by FTIR and X-ray diffraction (XRD). To identify the compounds responsible for reduction of silver ions, the functional groups present in plant extract were investigated by FTIR analysis and to observe the crystalline nature, structure was analyzed in XRD. Antibacterial activity of Ag NPs was performed by disc diffusion method against E. coli, Bacillus subtilis, Klebsiella pneumonia, Enterococcus faecalis and Staphylococcus aureus. The highest antimicrobial activity of silver nanoparticles synthesized by Murraya koenigii extracts was found against Klebsiella pneumoniae (20 mm). The result revealed that the plant has potent antibacterial activity and it can be used in drug preparation in future. Keywords: Murraya koenigii, FT-IR, Pseudomonas aeruginosa, green synthesis, silver nanoparticle Cite this Article Leon Stephan Raj T, Vijayakumari J, Kavitha S. Green Synthesis of Silver Nanoparticles Using Murraya Koenigii Leaf Extract and Their Antibacterial Potential. Research & Reviews: A Journal of Life Sciences . 2018; 8(3): 76–81p.