Mungbean Varieties Research Articles

Frequency and spectrum of viable mutations in mungbean (Vigna radiata (L.) Wilczek)

The mutagenic effect of gamma rays (200Gy, 300Gy, 400Gy, 500Gy, and 600 Gy), ethyl methane sulphonate (0.2%, 0.3%, 0.4%, 0.5%, and 0.6%) and sodium azide (1 mM, 2 mM and 3 mM) on frequency and spectrum of viable mutations in two mungbean varieties viz., WGG-42 and LGG-460 were investigated. A wide spectrum of viable mutations for stature, leaf, flowering/maturity duration, cotyledonary leaf, pod, seed size, seed color, and colour stem were identified in the M2 generation. The spectrum of viable mutants includeda total of 94 mutants in gamma gamma-irradiated population, 132 mutants in EMS and 45 mutants in SA treatments in WGG-42. In LGG-460, a total of 91 mutants in gamma gamma-irradiated population, 118 mutants in EMS treated population, and 45 mutants in SA treatments were observed. Viable mutation frequency increased with an increase in dose/concentration of gamma rays, EMS, and SA treatments in both genotypes. In both the genotypes, EMS showed the highest frequency of viable mutations than gamma rays and SA treatments. These viable mutants could be exploited as a variety or donor for bringing desirable traits into the otherwise well-adapted cultivars.

Genotypic Divergences of Important Mungbean Varieties in Response to Salt Stress at Germination and Early Seedling Stage

Genotypic Divergences of Important Mungbean Varieties in Response to Salt Stress at Germination and Early Seedling Stage

Solusi Recycle Sampah Organik dan Anorganik bagi Keluarga di Perumahan Taman Dramaga Permai 2 Bogor

Households are one of the contributors to organic and inorganic waste. Generally, the family needs to manage waste independently. The concept of waste recycling can be applied at the household level. The aim of waste management in the Dosen Mengabdi Reguler 2022 was to provide alternative solutions for processing organic and inorganic waste in the household based on the recycling concept. The activities were carried out from July‒November 2022 in one of the villages around the IPB campus locations, namely the Taman Darmaga Permai 2, Cihideung Ilir, Ciampea, Bogor. The core activities consist of discussions and demonstrations about compost, coenzymes, re-growing vegetable plants, and the culture of mung bean sprouts from Vima 1 and Vimil 1 varieties. About 42.9% of the 14 participants sorted household waste, and 28.6% managed waste. Compost from organic waste was harvested within one month after the process, while coenzymes were harvested after three months. Ecoenzymes were attractive to all participants as a solution for processing waste. About 50% of participants favor growing vegetables and 21.4% re-grow vegetable waste. Katuk and Welsh onion plants have 100% growth and 90% survival rates. All participants had never sprouted mung bean seeds. The sprouts can be harvested 1 to 3 days after sowing as needed. The Vima 1 mung bean variety has a sweet sprout taste and produces 657.9 g of sprouts from 100 g of seeds, while the Vimil 1 taste is slightly astringent with a lower sprout production of 543.8 g.

Genetic diversity and candidate genes for transient waterlogging tolerance in mungbean at the germination and seedling stages.

Mungbean [Vigna radiata var. radiata (L.) Wilczek] production in Asia is detrimentally affected by transient soil waterlogging caused by unseasonal and increasingly frequent extreme precipitation events. While mungbean exhibits sensitivity to waterlogging, there has been insufficient exploration of germplasm for waterlogging tolerance, as well as limited investigation into the genetic basis for tolerance to identify valuable loci. This research investigated the diversity of transient waterlogging tolerance in a mini-core germplasm collection of mungbean and identified candidate genes for adaptive traits of interest using genome-wide association studies (GWAS) at two critical stages of growth: germination and seedling stage (i.e., once the first trifoliate leaf had fully-expanded). In a temperature-controlled glasshouse, 292 genotypes were screened for tolerance after (i) 4 days of waterlogging followed by 7 days of recovery at the germination stage and (ii) 8 days of waterlogging followed by 7 days of recovery at the seedling stage. Tolerance was measured against drained controls. GWAS was conducted using 3,522 high-quality DArTseq-derived SNPs, revealing five significant associations with five phenotypic traits indicating improved tolerance. Waterlogging tolerance was positively correlated with the formation of adventitious roots and higher dry masses. FGGY carbohydrate kinase domain-containing protein was identified as a candidate gene for adventitious rooting and mRNA-uncharacterized LOC111241851, Caffeoyl-CoA O-methyltransferase At4g26220 and MORC family CW-type zinc finger protein 3 and zinc finger protein 2B genes for shoot, root, and total dry matter production. Moderate to high broad-sense heritability was exhibited for all phenotypic traits, including seed emergence (81%), adventitious rooting (56%), shoot dry mass (81%), root dry mass (79%) and SPAD chlorophyll content (70%). The heritability estimates, marker-trait associations, and identification of sources of waterlogging tolerant germplasm from this study demonstrate high potential for marker-assisted selection of tolerance traits to accelerate breeding of climate-resilient mungbean varieties.

Effect of foliar application of micronutrients on summer mungbean varieties on alluvial soil of West Bengal

A field experiment was carried out during the summer seasons of 2020 and 2021 at Jaguli Instructional Farm, BCKV, Nadia (West Bengal), in the Gangetic alluvial soil, to assess the effect of varieties and micronutrient foliar spray on growth parameters, yield components, yield and economics of mungbean. Results revealed that different varieties significantly promoted growth parameters and variety Meha was found to be superior. Growth parameters was significantly higher under molybdenum foliar spray over other micronutrients foliar spray. The maximum number of pods per plant, pod length, seeds per pod and test weight were found in plants receiving sodium molybdenum foliar spray at 0.05%. Maximum seed yield and stover yield were also recorded in the same treatment where 0.05% sodium molybdenum spraying was done at pre flowering and pod development stage. Variety showed significant variation in pods per plant, pod length, seeds per pod and test weight and were maximum in variety Meha. Both seed and stover yield were highest in case of variety Meha over others. The gross returns, net returns and benefit: cost ratio were maximum in Meha variety with foliar application of sodium molybdenum. Hence, variety Meha with foliar spray of molybdenum could be more effective in augmenting mungbean growth, yield and economics in the Gangetic plains of West Bengal in the summer season.

The plasma membrane-localized transporter VrNramp5 of Vigna radiata L. governs cadmium tolerance and accumulation in Arabidopsis thaliana

Cadmium (Cd) is a highly toxic non-essential element, which requires the transporters of essential and beneficial elements to enter the plant, and poses threat to plant growth and development. However, the mechanism of Cd uptake and accumulation in mungbean is limited. Here, we reported a Cd transporter VrNramp5 in mungbean, and its functional roles in response to Cd toxicity were conducted. The expression of VrNramp5 was down-regulated by Cd toxicity. VrNramp5 localized at plasma membrane based on subcellular localization analysis in epidermal cells of Nicotiana benthamiana. Heterologous expression of VrNramp5 in Arabidopsis thaliana promoted Cd accumulation, displayed the Cd sensitive phenotype including decreased root elongation and fresh weight, and VrNramp5 could diminish the Cd tolerance phenotype of AtNramp1-deficient mutant (atnramp1). Non–invasive micro–test showed that VrNramp5-overexpressing lines possessed higher Cd absorption capacity in root tips. Moreover, the Cd fluorescence probe staining revealed that more Cd accumulated in the leaf protoplasts of VrNramp5-overexpressing Arabidopsis. Those were consistent with the plants grown in the absence or presence of Cd (10 mg kg−1 or 20 mg kg−1) in soil. Taken together, VrNramp5 is responsible for plant Cd tolerance via regulating the Cd absorption capacity of roots and altering the intracellular distribution of Cd. Our findings provide a theoretical basis for further exploring the mechanism of Cd tolerance and breeding variety of mungbean with low Cd accumulation.

Deciphering PDH1's role in mung bean domestication: a genomic perspective on pod dehiscence.

Mung bean (Vigna radiata) stands as a crucial legume crop in Asia, contributing to food security. However, our understanding of the underlying genetic foundation governing domesticated agronomic traits, especially those linked to pod architecture, remains largely unexplored. In this study, we delved into the genomic divergence between wild and domesticated mung bean varieties, leveraging germplasm obtained from diverse sources. Our findings unveiled pronounced variation in promoter regions (35%) between the two mung bean subpopulations, suggesting substantial changes in gene expression patterns during domestication. Leveraging transcriptome analysis using distinct reproductive stage pods and subpopulations, we identified candidate genes responsible for pod and seed architecture development, along with Genome-Wide Association Studies (GWAS) and Quantitative Trait Locus (QTL) analysis. Notably, our research conclusively confirmed PDH1 as a parallel domesticated gene governing pod dehiscence in legumes. This study imparts valuable insights into the genetic underpinnings of domesticated agronomic traits in mung bean, and simultaneously highlighting the parallel domestication of pivotal traits within the realm of legume crops.

Impact of Various Levels of Salinity Stress on Growth Attributes of Some Salt-Sensitive and Tolerant Varieties of Mung Bean

Salinity stress poses a significant threat to crop productivity, and understanding the mechanisms of ion uptake and distribution in different cultivars is crucial for developing salt-tolerant varieties. This study investigates the patterns of ion uptake and distribution in salt-sensitive and salt-tolerant cultivars of Vigna radiata, commonly known as mung bean. Hydroponic experiments were conducted under controlled conditions to assess the responses of two contrasting cultivars to varying salt concentrations. The study reveals distinct differences in the ion uptake and distribution between salt-sensitive and salt-tolerant cultivars of V. radiata. Salt-sensitive cultivars exhibited increased sodium (Na+) accumulation in both roots and shoots under saline conditions, leading to disrupted ion homeostasis. In contrast, salt-tolerant cultivars demonstrated efficient ion regulation mechanisms, with a higher capacity for potassium (K+) retention and a lower Na+ to K+ ratio. The study also shed light on the involvement of ion transporters and channels in facilitating differential ion accumulation, highlighting the molecular mechanisms contributing to salt tolerance in Vigna radiata. These findings provide valuable insights into the adaptive strategies of salt-tolerant cultivars, offering a basis for targeted breeding programs aimed at enhancing salinity resilience in this economically significant legume. In summary, this research deepens our understanding of the intricate relationship between ion uptake and distribution in Vigna radiata varieties with varying salt tolerance. It lays the groundwork for developing robust crop varieties capable of thriving in saline environments.

Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach.

Yellow mosaic disease (YMD) remains a major constraint in mungbean (Vigna radiata (L.)) production; while short-duration genotypes offer multiple crop cycles per year and help in escaping terminal heat stress, especially during summer cultivation. A comprehensive genotyping by sequencing (GBS)-based genome-wide association studies (GWAS) analysis was conducted using 132 diverse mungbean genotypes for traits like flowering time, YMD resistance, soil plant analysis development (SPAD) value, trichome density, and leaf area. The frequency distribution revealed a wide range of values for all the traits. GBS studies identified 31,953 high-quality single nucleotide polymorphism (SNPs) across all 11 mungbean chromosomes and were used for GWAS. Structure analysis revealed the presence of two genetically distinct populations based on ΔK. The linkage disequilibrium (LD) varied throughout the chromosomes and at r2 = 0.2, the mean LD decay was estimated as 39.59 kb. Two statistical models, mixed linear model (MLM) and Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) identified 44 shared SNPs linked with various candidate genes. Notable candidate genes identified include FPA for flowering time (VRADI10G01470; chr. 10), TIR-NBS-LRR for mungbean yellow mosaic India virus (MYMIV) resistance (VRADI09G06940; chr. 9), E3 ubiquitin-protein ligase RIE1 for SPAD value (VRADI07G28100; chr. 11), WRKY family transcription factor for leaf area (VRADI03G06560; chr. 3), and LOB domain-containing protein 21 for trichomes (VRADI06G04290; chr. 6). In-silico validation of candidate genes was done through digital gene expression analysis using Arabidopsis orthologous (compared with Vigna radiata genome). The findings provided valuable insight for marker-assisted breeding aiming for the development of YMD-resistant and early-maturing mungbean varieties.

Efficacy of bio-rational insecticide for eco-friendly management of pod borer of mung bean

Pod borer species Maruca vitrata (Geyer) and Euchrysops cnejus (Fab.) are the major limiting constraints in mung bean. The present experiment was conducted in the experimental field of the Department of Entomology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during March to June 2021 to develop an ecofriendly management approach against pod borer attacking mung bean. Four eco-friendly biopesticides (Biomax 1.8 EC (Abamectin) @ 1.2 ml/L, Tracer 45 SC (Spinosad) @ 0.4 g/, Fytomax PM (0.1% Azadirachtin) @ 5.0 ml/L, Neem Seed kernel Extract (NSKE) @ 50.0 g/L) and clean cultivation were tested against pod borers of mung bean variety BU mug 4. An untreated control treatment was maintained for comparison. The marginal benefit-cost ratio of the treatments and the impact of the treatments on the abundance of beneficial arthropods in the experimental plots were recorded. The management approaches did not affect the abundance of beneficial arthropods in the bean field. The findings revealed that the lowest rate of pod infestation (1.9% by n/n and w/w) as well as the highest marketable yield (1.55 MT/ha) and marginal benefit- cost ratio (3.02) were obtained with the foliar spray with Tracer 45 SC (Spinosad) @0.4 g/L of water at 15 days interval. Tracer 45 SC (Spinosad) @ 0.4 g/L of water at 15 day intervals is an effective, suitable, and viable approach for suppressing pod borer complex without harm to beneficial arthropods in the mung bean field. Ann. Bangladesh Agric. (2023) 27 (1): 71-79

Effect of varying levels of nitrogen and phosphorus in combination with Rhizobium in different varieties of mung bean (Vigna radiata L.) Wilczek

Effect of varying levels of nitrogen and phosphorus in combination with Rhizobium in different varieties of mung bean (Vigna radiata L.) Wilczek

Evaluation of Genetic variability and viability association of eight Mungbean (Vigna radiata L.) varieties under different level of salinity stress

An investigation was carried to study the mean performance, variability and the associations between characters in mungbean. For this study eight mungbean genotypes viz., ADT 2, BM-2002-1, ML 5, AKM 4, Vishal, Vamban, TARM 1 and Utkarsh were used. The experiment was conducted in the Seed Science and Technology (sst) Laboratory and at Pot culture yard, Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar, Tamil nadu from September 2016 to December 2017 with four salt treatments (0, 2, 4 and 6 DSM-1) in ramdomized block design with three replications. Observation were on seven seedling characters such as germination percentage, seedling shoot length, seedling root length, seedling shoot fresh weight, seedling root fresh weight, seedling shoot dry weight, seedling root dry weight and seven quantitative characters such as days to first flowering, plant height, number of branches/plant, number of pods/plant, number of seeds/pod, 100 seed weight and seed yield/plant. Four elite genotypes were found to be superior based on the mean performance namely Utkarsh, TARM 1, Vamban and Vishal among the various levels of salinity.

Yield performance of mungbean varieties under different sowing dates

Yield performance of mungbean varieties under different sowing dates

Adaptability Study of Improved Mung bean (Vigna radiata) Varieties in Moisture Stress areas of Guji Zone, Southern Ethiopia

Mung bean (Vigna radiata L.), a green gram, is the most important short-duration legume crop for moisture-stressed areas globally. Because of the prolonged drought in soil stress areas in Ethiopia, drought-tolerant and early matured with little soil moisture crops are of utmost importance, and mung bean crop production is crucial in the lowland areas of the Guji zone. During the 2020–21 cropping season, a study was carried out at the Bore Agricultural Research Center's research mandate regions to assess the adaptation of mung bean cultivars. In order to assess eight characteristics—namely, days to 50% flowering, seed filling period, 90% physiological maturity, plant height, Number of pods per plant, Number of seeds per pod, seed yield, and hundred seed weight—for five mung bean varieties—Rasa, Shewa robit, NVL, Local, and Chinese—under rain-fed conditions at the Bore Agricultural Research Center on farms in the Guji Zone, a field experiment was set up in a randomized complete block design with three replications. With six rows per plot and a distance of 40 cm between rows and 10 cm between plants, each plot measured 2.4 meters by 4 meters. With the exception of plant height, which is not significantly different among varieties, the analysis of variances showed that Days to 50% blooming, Days to 90%maturity, number of pods per plant, number of seeds per pod, hundred seed weight, and grain output per hectare changed as impacted by variety. The highest grain yield (1014.04kg/ha) was recorded from the Rasa variety, followed by the local cultivar (938. 45kg/ha), whereas the minimum grain yield value (715.67kg/ha) was recorded by the variety NVL. Hence, the variety Rasa was best adapted in the study areas and it will be used for production.

Evaluation of Induced Genetic Variability for Yield and Yield Contributing Traits in M4 Generation of Mungbean (Vigna radiata (L.) Wilczek)

The goal of the current study was to determine how much genetic variability there was for yield and traits that contributed to yield in two different mungbean varieties, WGG-42 and LGG-460, following the induction of mutations through chemicals (ethyl methane sulphonate and sodium azide) and physical (gamma rays) mutagens. In Rabi, 2018-19, in RBD with three replications in M4generation, fifty-five mutant lines selected from M3 progenies (36 in WGG-42 and 19 in LGG-460) were assessed alongside the two parents. Number of primary branches per plant, number of clusters per plant and number of pods per plant all showed high GCV and PCV. All the characters under study have high heritability. High heritability coupled with high genetic advance as per cent of mean was recorded for plant height, number of primary branches per plant, number of clusters per plant, number of pods per plant, pod length and seed yield per plant indicating that the genetic variances for these characters are probably owing to their high additive gene effects. Our study indicates promising avenues for future mungbean breeding programs to enhance these characteristics.

Performance of mungbean in Response to zinc and iron through agronomic mechanism of biofortification

A field experiment was conducted at College of Agriculture, Agriculture University, Jodhpur in kharif season during 2020 and 2021 to study the effect of agronomic biofortification of zinc and iron on growth, yield and quality of mungbean (Vigna radiata (L.) Wilczek) varieties. The experiment was laid out in a split plot design, comprised with two varieties namely GM-7 and MH-421 and three treatments of foliar spray of iron (Fe) and urea in main-plots and four treatments included seed inoculation by zinc solubilizing bacteria with soil application of zinc (Zn) in a combinations were assigned to sub-plots. In this way, the experiment had twenty four treatment combinations were replicated thrice. Mung bean variety ‘MH-421’ had performed prominently with respect to all growth and yield parameters, while taller height was recorded under variety ‘GM-7’ during both the years as well as pooled basis of experimentations. Biofortified treatment of iron with urea spray and zinc significantly higher growth and yield attributes were recorded under treatment foliar spray of 0.5% Fe + 2% urea at flower initiation and pod formation stages and seed inoculation with ZSB + soil application of higher dose of zinc sulphate i.e. ZSB (SI) + 25 kg ZnSO4/ha, which remained at par with foliar spray of 0.5% Fe + 2% urea at flower initiation stage and ZSB (SI) + 20 kg ZnSO4/ha at 25, 50 DAS and at harvest stage of mungbean, respectively.

Effect of foliar application of micronutrients and urea on mungbean varieties

Experiment was carried out to at the Agronomy field of Patuakhali Science and Technology University (PSTU). The treatments comprised of four variety and three fertilizer including control. Varieties were V1= BINA Mung-8, V2=BARI mung-2, V3=BARI mung-5 and V4=BARI mung-6 and treatments were T0 = control, T1 =micro nutrient, T2= micronutrient and urea. The experiment was laid-out in the Randomized Complete Block Design (RCBD) with three replications. The maximum plant height was found at BARI mung-6 (29.7). Maximum nodule number was found at BARI mung-6 variety (5.90). The number of effective nodule was highest at BARI mung-6 variety (2.36). In case of no of seed per pod and 1000-seed weight, BARI mung-6 variety gave highest value and those were 11.25 and 70.5 g respectively. Maximum plant height, nodule number, number of effective nodule and number of pod per plant were found at T2 treatment. The pod length, seed per pod and 1000-seed; T2 treatment gave the highest value and those were 5.5 cm, 11.25 and 65.64 g respectively. In case of plant height, chlorophyll content, number of nodule, number of effective nodule, pod per plant, pod length, pod weight/m2, seed per pod and 1000-seed weight, combined effecr of BARI mung-6 x micronutrient and urea (T2) gave highest value and those were (29.95, 47.5, 6.17, 2.40, 6.20, 6.20, 76.75, 11.20) cm and 68.45 g respectively. This result revealed that mungbean variety BARI mung-6 and T2 treatment performed best in combination (V4T2).

Morphophysiological Changes Of Mungbean Under Different Water Regimes

Drought stress can severely affect crop growth and productivity by altering several physiological processes. This experiment was carried out to explore the drought tolerance ability of four mungbean varieties based on their water relation and performance of some growth parameters. The experiment was conducted in pot at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur from 27th March to 15th May 2017, under a semi-controlled (vinylhouse) condition. Three different water regimes 50 to 60% field capacity (FC), 70 to 80% FC, 90 to100% FC and four mungbean varieties namely BARI Mung-5, BARI Mung-6, BU mug 2, BU mug 4 were used as treatment variable. Among the three water regimes, 50%-60% FC was considered as the severe drought stress. Results indicated significant variations in different traits of both water and growth parameters of the varieties under severe drought stress (50%-60% FC). Among the four mungbean varieties BARI Mung-6 showed superior performance with higher xylem exudation, chlorophyll content, shoot dry matter and lower water uptake capacity at 50%-60% FC (severe drought stress) whereas BU mug 2 showed the lowest performance. Results of this experiment conclude that BARI Mung-6 can be considered as a more water stress tolerant variety than the other three and recommended for cultivation under water limited conditions.
 Bangladesh J. Agril. Res. 47(3): 257-267, September 2022

Yield and Mineral Composition among Mungbean [Vigna radiata (L.) R. Wilczek] Genotypes Grown in Different Agroecologies in East Africa

Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] also referred to as green gram is a key crop especially in the marginal areas of East Africa. It is rich in micronutrients and protein and thus can help ameliorate malnutrition if incorporated into diets. This study was conducted to evaluate the performance of 26 fixed elite mungbean breeding genotypes for yield and micronutrient composition across different locations in East Africa (Uganda, Kenya, and Tanzania). The genotypes displayed significant variability for nutritional, phenology, and yield-related traits across test environments. Significant genotype effects were observed for most of the traits except for average yield per plant and dry matter content ( P < 0.05). Random effect of environment was significant for all traits, and significant GXE was observed for all traits except for dry matter and iron content ( P < 0.05). Moderate to high broad-sense heritability (H2) was found among traits except for dry matter content which was low (H2 = 10.4%). There was a significant and positive correlation between Fe and Zn (r = 0.58), and Fe and Ca (r = 0.46), indicating the potential to enhance these traits simultaneously through breeding and/or selection. However, the correlation between yield and nutrients (iron, zinc, and calcium) was negative. Environments KAT_SR_2019, KYM_LR_2020, and KYM_LR_2020 were found to be discriminating (informative) and representative of grain yield, calcium, and zinc content, respectively. Genotypes AVMU 1679, AVMU 1685, and AVMU1686 combined both stability and high micronutrient content, while the high yielding and stable genotypes were AVMU 1689, AVMU 1681, and AVMU 16102. The identified genotypes need to be assessed for farmer preference in on-farm trials before they can be recommended for release as new varieties. Additionally, these genotypes will be useful in future breeding efforts as donors aimed at developing nutrient-dense and high yielding mungbean varieties.

Integration of comparative transcriptomics and WGCNA characterizes the regulation of anthocyanin biosynthesis in mung bean (Vigna radiata L.).

Mung bean is a dual-use crop widely cultivated in Southeast Asia as a food and medicine resource. The development of new functional mung bean varieties demands identifying new genes regulating anthocyanidin synthesis and investigating their molecular mechanism. In this study, we used high-throughput sequencing technology to generate transcriptome sequence of leaves, petioles, and hypocotyls for investigating the anthocyanins accumulation in common mung bean variety as well as anthocyanidin rich mung bean variety, and to elucidate their molecular mechanisms. 29 kinds of anthocyanin compounds were identified. Most of the anthocyanin components contents were significantly higher in ZL23 compare with AL12. Transcriptome analysis suggested that a total of 93 structural genes encoding the anthocyanin biosynthetic pathway and 273 regulatory genes encoding the ternary complex of MYB-bHLH-WD40 were identified, of which 26 and 78 were differentially expressed in the two varieties. Weighted gene co-expression network analysis revealed that VrMYB3 and VrMYB90 might have enhanced mung bean anthocyanin content by inducing the expression of structural genes such as PAL, 4CL, F3'5'H, LDOX, and F3'H, which was consistent with qRT-PCR results. These findings are envisaged to provide a reference for studying the molecular mechanism of anthocyanin accumulation in mung beans.