As renewable energy grows in the heavy-duty truck sector, exploration of diverse solutions with varied energy types and powertrain configurations becomes a necessity. While most studies focus on powertrain configurations in tractors, this study takes a different approach: propose and evaluate new plug-in hybrid configurations with multiple power sources for heavy-duty trucks. The tractor retains its engine powertrain, while the trailer is equipped with electric axle(s), forming a fuel-electricity hybrid propulsion system. The specifications of the electric propulsion system are optimized, using a multi-objective particle swarm optimization algorithm and dynamic programming control algorithm to evaluate the energy consumption and total cost of ownership (TCO). The results highlight the potential of the configuration with three electric axles and single reduction gear in regard to vehicle energy consumption. Conversely, the configuration with one electric axle and a two-speed transmission exhibits the lowest TCO. Under the China World Transient Vehicle Cycle, the fuel consumption of the diesel truck is reduced by up to 44.59 % with the use of the optimized hybrid configuration. The truck's TCO can be then reduced by up to 832 thousand CNY in one-million-kilometer operation. For an actual road operation, these figures change to 34.79 % and 284 thousand CNY, respectively.