Recently in Poland there has been an intensive process of transforming the economic structure of rural areas, which manifests itself, inter alia, in changing the occupational structure of its inhabitants. Development of non-agricultural ways of farming in rural areas leads to creation of rural areas of multifunctional character, with population growth, where the role of agriculture as a source of income of inhabitants is decreasing. On the other hand, in the marginal areas, with still dominant agricultural function, there is a significant population loss. One of the effects of the observed process are changes in rural settlements. The aim of the presented research is to deepen the existing knowledge on the evolution of spatial distribution of rural population depending on the level of socio-economic development of the region and structural features of the level achieved. The authors used research conducted within the framework of monitoring rural development in Poland. They compared them with the statistical database of the Central Statistical Office, which made it possible to distinguish growing and depopulating villages. The results of the study confirmed that the spatial differentiation of population in rural areas undergoes constant evolution and its effect is a decrease of population in areas located far from larger cities and an increase in suburban areas.