Meditation and other alternative practices have long been known to promote various health benefits, presumably by fostering a blood and tissue environment that enhances resilience to stress. Here, we used an integrated, multidisciplinary approach that couples quantitative electroencephalography (qEEG), biometrics, molecular, and biochemical data at multiple time points to investigate the impact of mind-based interventions on the body in a cohort of twins during an intensive week-long meditation retreat. We aim to show the feasibility of a novel design aimed to address individual changes controlling for intersubject trait variation (via twin control) and explore the role of genetic background on multi-omic factors during meditation. Interestingly, similar patterns of change in brain activity, network properties, and complexity were observed in the twin sets. These similarities were not observed in mismatched twin pairs. Heart rate dynamics studies showed alignment among twin pairs that was not present between unmatched pairs. In addition, changes in gene expression, metabolites, and cytokines present in blood plasma and associated with specific meditative states showed patterns of change associated by timepoint. Twin sets were similar in multiple domains before the start of the retreat, showed larger divergence at the mid-point, and looked more similar by the end of the retreat. To our knowledge, this study is novel within the twin research paradigm and is a first step towards exploring the effects of meditation in twins. InnerScience Research Fund. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.