The objective of this work is to evaluate the influence of over-ageing on the high cycle fatigue behaviour of the AlSi7Cu0.5Mg0.3 cast aluminium alloy. It is generally accepted that over-ageing has a large influence on the behaviour of these materials, however its effect on the fatigue strength is less understood. This work provides an experimental investigation of the effect of over-ageing on high cycle fatigue strength in both tensile and torsional loading conditions. It is shown that the torsional fatigue strength is more sensitive to over-ageing when compared to the tensile fatigue strength and that the fatigue defect sensitivity is negligible for torsional loads, in contrast to tensile loading conditions. The experimental results indicate that this is due to a change in the crack initiation mechanism, going from initiation in the matrix for torsion loads to initiation from casting defects for tensile loads. An original two-steps approach is proposed to rationalise the effect of over-ageing on the fatigue strength, with particular emphasis on decoupling the respective contributions from defects and the mechanical properties. This approach is used to build normalised Kitagawa-Takahashi diagrams that highlights the reduced defect sensitivity under tensile loads for the most severe over-ageing condition.