This study focuses on measuring the process dynamics for the multistage flash desalination process (MSF) in an industrial unit with a capacity of 4546 m 3/d. This is a novel addition to the literature because previous studies are limited to theoretical analysis of process dynamics or controller tuning, as well as conceptual design of conventional or advanced control systems. The measurements evaluate the performance of seven control loops, which include the pressure, temperature, and flow rate of the heating steam; the pressure of the vacuum ejector; and flow rates of the brine recycle, make-up seawater, and cooling seawater. All measurements start from steady-state conditions. The system is then set on manual where all control units are disengaged. Subsequently, only one control valve is adjusted by ± 15% of its steady-state setting. A total of 14 experiments were performed involving simultaneous measurements of the system variables. Measurements showed non-linear behavior where increasing or decreasing the valve settings did not provide similar trends. Analysis of results shows that one of the most sensitive variables is the distillate level in the last stage: the distillate trays either were flooded or became dry. The brine level in the last flashing stage was also found to be sensitive to valve settings where level increase resulted in higher product salinity. The results and analysis presented provide a better understanding in system fault analysis which could be caused by improper operating conditions. These data are essential to propose, design, and evaluate advanced/comprehensive control systems for the MSF process.