Rapid growth of renewable energy sources (RES) in the generation capacity mix poses substantial challenges on the operation of power systems in various time scales. Particularly in the intra-hour time scale, the interplay among variability and uncertainty of RES, unexpected transmission/generation outages, and short dispatch lead time cause difficulties in generation-load balancing. This paper proposes a method to quantify the intra-hour flexibility region. A robust security-constrained multiperiod optimal power flow model is first constructed to quantify the frequency, magnitude, and intensity of insufficient flexibility. The randomness of RES is captured by uncertainty sets in this model. The N-k contingency, spinning reserve, and corrective control limit constraints are included. This model is then cast into a two-stage robust optimization model and solved by the column-and-constraint generation method. The emergency measures with a least number of affected buses are derived and subsequently assessed by the postoptimization sensitivity analysis. Finally, the operational flexibility region is determined by continuous perturbation on the RES penetration level and the forecast error. The IEEE 14-bus system and a realistic Chinese 157-bus system are used to demonstrate the proposed method.