Purpose The purpose of this paper is to develop a new simple technique to synthesize graphene film on a flexible polyethylene terephthalate (PET) substrate and applied as a strain sensor. Design/methodology/approach Graphene film was synthesized using laser treatment of graphene oxide (GO) film deposited on PET substrate. A universal laser system was used to simultaneously reduce and pattern the GO film into laser reduced graphene oxide (LRGO) film. Findings The laser treatment synthesizes a multilayer graphene film with overlapped flakes, which shows structure integrity, mechanical flexibility and electrical conductivity of 1,330 S/m. The developed LRGO/PET film was used to fabricate a high sensitivity strain sensor. The sensitivity and temperature dependency of its gauge factor (GF) was examined at applied strains up to 0.25 per cent and operating temperatures up to 80°C. The fabricated sensor shows stable GF of approximately 78 up to 60°C with standard error of the mean not exceeding approximately ± 0.2. Originality/value The proposed method offers a new simple and productive technique of fabricating large-scale graphene-based flexible devices at a low cost.