This study aims to examine how and in what ways pre-service teachers’ identities are constructed and developed in student-teaching experiences. In this study, two Taiwanese EFL university pre-service teachers participated in a year-long 2019-2020 case study at Southern University. Data consisted of pre-service teacher multicultural attitudes, the transcriptions of course documents, and open-ended interviews. The study suggests that the pre-service teachers’ beliefs play a significant role in shaping their understanding of language learning and teaching. Additionally, the experiences pre-service teachers have in diverse cultural settings shape their identities and inform their future teaching practices as they develop the ability to connect with their students and offer valuable insights. The results suggest that future pre-service teacher education programs should nurture pre-service teachers’ multicultural awareness and cultural teaching strategies before and during teaching the students.