Film Mulching Research Articles

Combined effects of biochar and biodegradable mulch film on chromium bioavailability and the agronomic characteristics of tobacco

Biochar (BC) and biodegradable mulch film (BMF) are both commonly used means of production in agriculture. In recent years, most studies have focused on the effects of BC or BMF on soil heavy metal pollution, while they have neglected the combined effects. In this study, a pot experiment was conducted to examine the impacts of BMF, BC, and combined BMF and BC (CMB) on the mobility of chromium (Cr) and the agronomic characteristics of flue-cured tobacco. Compared with the control, BMF, BC, and CMB significantly reduced the concentrations of diethylenetriamine pentaacetic acid (DTPA) extractable Cr in soils by 29.07–29.75%, 45.35–48.54%, and 34.21–37.92%, respectively. In comparison to the application of BMF and BC alone, co-application reduced the availability of Cr in soil via increasing the adsorption of soil Cr and soil enzyme activity, which resulted in the decrease of Cr content and bioconcentration factor and in plants. Moreover, the combined application increased the plant height, stem diameter, leaf area, total root area, root tip number, and root activity of tobacco, which leaded to increase in leaf and root biomass by 11.40–67.01% and 23.91–50.74%, respectively. Therefore, the application of CMB can reduce the heavy metal residues in tobacco leaves and improve tobacco yield and quality.

Corrigendum to “Macro- and microplastic accumulation in soil after 32 years of plastic film mulching” [Environ. Pollut. 300, (2022), 118945]

Corrigendum to “Macro- and microplastic accumulation in soil after 32 years of plastic film mulching” [Environ. Pollut. 300, (2022), 118945]

Biodegradable and multifunctional black mulch film decorated with darkened lignin induced by iron ions for “green” agriculture

High-value utilization of bleached lignin has been widely used in different fields, whereas the investigation on darkened lignin in composite materials was often ignored. In this work, a sort of eco-friendly and structurally robust sodium carboxymethyl cellulose (CMC)/polyvinyl alcohol (PVA)/sodium lignosulfonate (SLS) black composite mulch film was elaborately designed. The chelation and redox reaction effect between Fe ions and SLS lead to the formation of a more quinones structure on lignin, darkening both lignin and the mulch films. The chelation effect between Fe ions and biopolymer formed three-dimensional structures, which can be used as sacrifice bonds to dissipate energy and improve the mechanical properties of the composite films. In particular, the maximum elongation at break and toughness increased from 48.4 % and 1141 kJ/m3 for the CMC/PVA film to 210.9 % and 1426 kJ/m3 for the optimized CMC/PVA/SLS/Fe black mulch film, respectively. In addition, the optimized black mulch film also possesses good soil water retention, thermal preservation effect, controlled urea release, and well biodegradability. This work offered a novel strategy for designing eco-friendly black mulch with reinforced mechanical strength, slow-release urea, soil moisture retention, and heat preservation performances.

Fabrication of a straw-based degradable mulch film with magnetically controlled-release glyphosate

To solve three main problems in agriculture (plastic mulch pollution, difficult straw recycling, and low utilization efficiency of pesticides), a multifunctional mulch film (MF) was fabricated. In this study, corn straw was mixed with xanthan gum, glyphosate (Gly), Fe3O4, and MIL-100(Fe) to obtain a MF named as SMFG with magnetically controlled-release Gly. To improve the hydrophobicity and stability of SMFG, the surface was coated by amino silicone oil (ASO) to get SMFG@ASO. SMFG@ASO had well insulation and moisture retention properties close to polyethylene MF. Meanwhile, the weight loss ratio of SMFG@ASO increased with time and could reach 59% underground in 120 d, indicating the good biodegradation behavior of SMFG@ASO. Importantly, pot experiment showed that SMFG@ASO could significantly promote growth of crop and inhibit grass. Besides, earthworm experiment illustrated that SMFG@ASO possessed a high biosafety and could increase the relative abundance of beneficial bacteria (Flavobacterium) from 0.08 to 0.21 and soil quality. Thus, this study provides a new method to prepare a straw-based degradable MF for resolving the problems of plastic pollution and waste of straw resources simultaneously to realize the integration of herbicide and MF.

Study on the Hole-Forming Performance and Opening of Mulching Film for a Dibble-Type Transplanting Device

In order to improve the quality of transplanting devices and solve the problems of the poor effect on soil moisture conservation and more weeds easily growing due to the high mulching-film damage rate with an excessive number of hole openings, we developed a dibble-type transplanting device consisting of a dibble-type transplanting unit, a transplanting disc, and a dibble axis. The ADAMS software Adams2020 (64bit) was used to simulate and analyze the kinematic track of the transplanting device. The results of the analysis show that, when the hole opening of the envelope in the longitudinal dimension was the smallest, the transplanting characteristic coefficient was 1.034, the transplanting angle was 95°, and the transplanting frequency had no influence. With the help of the ANSYS WORKBENCH software Ansys19.2 (64bit), an analysis of the process of the formation of an opening in the mulching film and a mechanical simulation of this process were completed. The results indicate that, when the maximum shear stress of the mulching film was the smallest, the transplanting characteristic coefficient was 1.000, the transplanting frequency was 36 plants·min−1, and the transplanting angle was 95°. In addition, the device was tested in a film-breaking experiment on a soil-tank test bench to verify the hole opening in the mulching film. The bench test showed that, when the longitudinal dimension was the smallest, the transplanting characteristic coefficient was 1.034, the transplanting frequency was 36 plants·min−1, and the transplanting angle was 95°. When the lateral dimension was the smallest, the transplanting characteristic coefficient was 1.034, the transplanting frequency was 36 plants·min−1, and the transplanting angle was 90°. The theoretical analysis, kinematic simulation, and soil-tank test results were consistent, verifying the validity and ensuring the feasibility of the transplanting device. This study provides a reference for the development of transplanting devices.

Fungal necromass carbon contributes to organic carbon sequestration within soil macroaggregates under manure application combined with plastic film mulching

Fungal necromass carbon contributes to organic carbon sequestration within soil macroaggregates under manure application combined with plastic film mulching

Revalorization of Yerba Mate Residues: Biopolymers-Based Films of Dual Wettability as Potential Mulching Materials.

Biodegradable mulching films are a very attractive solution to agronomical practices intended to achieve more successful crop results. And, in this context, the employment of agricultural and industrial food residues as starting material for their production is an alternative with economic and environmental advantages. This work reports the preparation of bilayer films having two different wettability characteristics from three bio-derived biopolymers: TEMPO-oxidized cellulose nanofibers isolated from infused Yerba Mate residues, Chitosan and Polylactic acid. The infused Yerba Mate residues, the isolated and oxidized cellulose nanofibers, and the films were characterized. Nanofibrillation yield, optical transmittance, cationic demand, carboxyl content, intrinsic viscosity, degree of polymerization, specific surface area and length were studied for the (ligno)cellulose nanofibers. Textural and chemical analysis, thermal and mechanical properties studies, as well as water and light interactions were included in the characterization of the films. The bilayer films are promising materials to be used as mulching films.

A three-year record of CO2, CH4 and N2O emissions in maize fields influenced by mulching methods on the Loess Plateau, China

Exploring optimal mulching methods for maize production is critical to achieving a balance between maximizing yield and reducing negative environmental impacts in dryland agriculture. Therefore, a 3-yr field experiment was conducted to investigate the effects of three mulching methods—biodegradable plastic film mulching (BM), maize straw mulching (SM), and no mulching (NM)—on spring maize yield, greenhouse gas (GHGs) emissions (CO2, CH4, and N2O), soil factors, the net ecosystem GHG balance and greenhouse gas emission intensity (GHGI) on the Loess Plateau of China. The result showed indicated that CO2 and N2O are the primary sources of GHGs in dryland maize production. The yield of BM significantly increased 1.43–1.99 t ha−1 compared to NM, CO2 emissions (on average 32.83%) compared to the NM. And BM showed suitability for N2O emissions (3.7–11.2%). SM increased CO2 and N2O emissions (by 9.43–13.17% and 24.96–33.64%, respectively) compared to the NM. Structural equation model analysis showed that soil temperature was the most direct factor to affect GHGs. SM was the sink of net ecosystem GHGs, BM and NM were the source of net ecosystem GHGs. These results indicated that mulching emerges as a promising strategy for dryland agriculture. BM method can be used when the primary goal was to improve yield, while SM method is a better choice when the primary goal was to improve soil organic carbon sequestration and reduce net GHGs emissions. This study clarified the impacts of mulching methods on three GHGs emissions to provide information support for sustainable agriculture on the Loess Plateau.

Plastic film mulching increases crop yields and reduces global warming potential under future climate change

Identifying suitable cropping systems and planting areas with high yields and low greenhouse gas (GHG) emissions to adapt to climate change is essential for sustainable agricultural development. The DeNitrification-DeComposition (DNDC) model and the Coupled Model Inter-comparison Project Phase 6 (CMIP6) dataset were used to evaluate the impact of climate scenarios on the crop yields and global warming potential (GWP) of maize, winter wheat, and potato under plastic film mulching (PM) and without mulching (CK) systems on the Loess Plateau for 2020–2100. The result showed that the DNDC model could simulate crop yields and carbon dioxide, nitrous oxide, and methane emissions (adjusted R2 > 0.55, normalized root mean square error (nRMSE) < 0.32). Under multiple emission scenarios, the yields of maize and winter wheat increased linearly with time (p < 0.05), the GWP decreased linearly with time (p < 0.05), and the coefficients of variation of both were lower for the PM than CK, indicating that the impact of future climate change on PM system was small and can achieve synergy between food security and carbon reduction. The results of regional divergence characteristics suggested that areas with precipitation below 400 mm were suitable for maize cultivation, especially the PM system, while areas with precipitation above 400 mm were suitable for winter wheat cultivation – both of which could achieve the goals of high yield and low GHG emissions. This study presents an overview of spatiotemporal changes in production and ecological effects, providing a basis for developing agricultural management practices and cultivation areas to address climate change, and could promote the development of dryland agroecosystems in China in a green and sustainable direction.

Effect of Film Mulching Age and Organic Fertilizer Application on the Distribution Characteristics of Microplastics in the Soil of a Peanut Field

The large input of mulch film and organic fertilizer have led to increasingly serious microplastic pollution in farmland soil of China. In this study, the microplastic pollution of peanut farmland in Dezhou City, Shandong Province was investigated. The effects of different mulching years (0, 3, 5, and 8 years) and organic fertilizer application on the abundance, particle size, color, and shape of microplastics in farmland soil were analyzed. The results showed that the average abundances of microplastics in peanut soil were 65.33, 316.00, 1 098.67, and 1 346.34 n·kg-1, respectively, after 0, 3, 5, and 8 years of film mulching. The abundance of microplastics decreased with the increase in soil depth. The abundance of microplastics in 0-10, 10-20, and 20-30 cm topsoil was 1 076.00, 603.5, and 440.25 n·kg-1, respectively, and the abundance of microplastics increased significantly with increasing years of film mulching and organic fertilizer application (P<0.05). The particle size of microplastics in the sample plot <1 mm accounted for 77.30% of the total content, and with the increase in film mulching age, the proportion of microplastics with small particle size (<1 mm) increased significantly (P < 0.05). With the increase in soil depth, the proportion of microplastics with small particle size also gradually increased, whereas the application of organic fertilizer had no significant effect on the particle size of microplastics. The color of microplastics in the plot was mainly transparent (49.77%), followed by black (16.35%) and white (16.27%). The planting age and organic fertilizer application had no significant effect on the color of microplastics in the soil (P > 0.05), but the mulching age significantly increased the proportion of transparent microplastics. The abundance proportion of the five types of microplastics were 49.77%, 25.41%, 19.15%, 3.26%, and 2.41%, respectively. These field soil microplastics were mainly composed of polyethylene (PE), polypropylene (PP), and polystyrene (PS) polymers, accounting for 21.37%, 18.57%, and 19.77% of the total, respectively. Therefore, microplastics were widely present in the soil of the peanut field cultivated layer in Dezhou, Shandong, and the applications of mulch film and organic fertilizer were the main source. This study provides an important basis for the prevention and control of soil microplastic pollution in peanut fields.

Polyhydroxybutyrate (PHB) bioplastic characterization from the isolate Pseudomonas stutzeri PSB1 synthesized using potato peel feedstock to combat solid waste management

The global fossil-based plastic industry is at a crucial point, requiring bio-based polymers. Polyhydroxybutyrate (PHB) is formed under nutritional stress conditions; however, efficient bacteria and cheap substrates are needed. This study found that potato peel, a cheap and abundant carbon source, can improve PHB production. PHB crotonic assay screened 11 Sudan Black B and Nile red positive isolates for PHB production. PSB1, a Pseudomonas stutzeri PSB1 (MN539619) yields 2.39 g/L maximal PHB. PHB generation from high-carbon agro-wastes using Pseudomonas stutzeri PSB1was 3.09 g/L for potato peel. Plackett-Burman and CCD, a statistical optimization method, increased PHB production. After optimizing all CCD variables, potato peel, Urea, and RPM were found to increase PHB production (6.1 g/L). The optimized parameters extracted 3.98 g/L of PHB from 6.36 g/L of dry biomass of Pseudomonas stutzeri PSB1. FT-IR, NMR, TGA, and DSC were used to characterize and compare the extracted PHB biopolymer to Sigma Aldrich PHB powder. Extracted PHB-bioplastic was tested for tensile strength and Elongation at break. Based on the above criteria, the recovered PHB biopolymer from Pseudomonas stutzeri PSB1 is a good alternative to agricultural mulch films and non-biodegradable plastics.

Occurrence, potential sources, and ecological risks of traditional and novel organophosphate esters in facility agriculture soils: A case study in Beijing, China

Although traditional organophosphate esters (OPEs) in soils have attracted widespread interest, there is little information on novel OPEs (NOPEs), especially in facility agriculture soils. In this work, we surveyed 11 traditional OPEs, four NOPEs, and four corresponding organophosphite antioxidant precursors (OPAs) for the NOPEs in soil samples collected from facility greenhouses and open fields. The median summed concentrations of traditional OPEs and NOPEs were 14.1 μg/kg (range: 5.38–115 μg/kg) and 702 μg/kg (range: 348–1952 μg/kg), respectively, in film-mulched soils from greenhouses. These concentrations were much higher than those in soils without mulch films, which suggests that OPEs in soils are associated with plastic mulch films. Tris(2,4-di-tert-butylphenyl) phosphate, which is a NOPE produced by oxidation of (2,4-di-tert-butylphenyl) phosphite, was the predominant congener in farmland soils, with concentrations several orders of magnitude greater than those of traditional OPEs. Comparisons of OPEs in different mulch films and the corresponding mulched soils revealed that degradable and black films caused more severe pollution than polyethylene and white films. Traditional OPEs, including tris(2-ethylhexyl) phosphate and tricresyl phosphate, exhibited moderate risks in farmland soils, especially in film-mulched soils. NOPEs, including trisnonylphenol phosphate, posed high ecological risks to the terrestrial ecosystem. Risk evaluations should be conducted for a broad range of NOPEs in the environment.

Response of garlic (Allium sativum L.) to the combined toxicity of microplastics and arsenic

The extensive utilization of mulch films in agricultural settings, coupled with the persistence of microplastic remnants in soil following the natural degradation of plastics, has given rise to detrimental microplastic impacts on crops. Arsenic (As) contamination in the environment is known to accumulate in crops through aquatic pathways or soil. Garlic (Allium sativum L.), a globally popular crop and seasoning, contains alliin, a precursor of its flavor compounds with medicinal properties. While alliin exhibits antimicrobial and antioxidant effects in garlic, its response to microplastics and arsenic has not been thoroughly investigated, specifically in terms of microplastic or As uptake. This study aimed to explore the impact of varied stress concentrations of microplastics on the toxicity, migration, and accumulation of As compounds. Results demonstrated that polystyrene (PS) fluorescent microspheres, with an 80 nm diameter, could permeate garlic bulbs through the root system, accumulating within vascular tissues and intercellular layers. Low concentrations of PS (10 and 20 mg L−1) and As (2 mg L−1) mitigated the production and accumulation of reactive oxygen species (ROS) and antioxidant enzymes in garlic. Conversely, garlic exhibited reduced root vigor, substance uptake, and translocation when treated with elevated As concentrations (4 mg L−1) in conjunction with PS concentrations of 40 and 80 mg L−1. An escalation in PS concentration facilitated As transport into bulbs but led to diminished As accumulation and biomass in the root system. Notably, heightened stress levels weakened garlic's antioxidant defense system, encompassing sulfur allicin and phytochelatin metabolism, crucial for combating the phytotoxicity of PS and As. In summary, PS exerted a detrimental influence on garlic, exacerbating As toxicity. The findings from this study offer insights for subsequent investigations involving Liliaceae plants.

Effects of different colors of plastic-film mulching on soil temperature, yield, and metabolites in Platostoma palustre

Platostoma palustre is an annual herb and an important medicinal and edible plant in southern China. Plastic-film mulching is an effective agronomic practice in the cultivation system of P. palustre, of which black-film mulching is the most common. However, fewer researches have been focused on the use of other colors of plastic films in P. palustre cultivation. In this study, different colors (white, black, red, and green) of plastic film were adopted, and the effects of different colors of plastic film mulching on the soil temperature, yield, and metabolites of P. palustre were investigated. The results showed that the fresh weight of a single plant of the green film treatment was significantly higher than that of the white film treatment (n = top 28). Based on the results of three temperature measurements, the soil temperature was almost the highest in the red film treatment and lowest in the white film treatment. The metabolomic analysis revealed that a total of 103 differential metabolites were identified. Among these, the gluconic acid, deoxyribose, and N-Acetylmannosamine in the red film treatment presented the highest abundance compared with the other treatments, meanwhile, the abundances of the five monosaccharides in the red film treatment were significantly higher than those of the green film treatment. Moreover, the sucrose, trehalose, and D-(+)-trehalose in the green film treatment exhibited the highest abundance, and the abundances of eight different amino acids in the red film treatment were almost the lowest while those in the black film treatment were almost the highest. Further analysis of the membership function values indicated that the black and red film treatments might be more suitable for the cultivation and quality production of P. palustre in comparison with the other two treatments. This study will provide a theoretical basis for improving the efficient cultivation technology of P. palustre and forming a theoretical system of P. palustre film mulching cultivation.

Effects of Biodegradable Plastic Film Mulching on the Global Warming Potential, Carbon Footprint, and Economic Benefits of Garlic Production

This paper clarifies the farm applicability and feasibility of spreading biodegradable plastic film mulching for garlic production to ensure the green and sustainable development of the garlic industry. We set up a field trial of garlic planting with biodegradable plastic film mulching (BM) and plastic film mulching (PM), using no film mulching (CK) as the control, and measured CH4 and N2O emissions in the garlic fields. The yield-scaled global warming potential, carbon footprint, and net ecosystem economic benefit (NEEB) were used to assess the comprehensive impact of the different treatments. Compared to the CK, film mulching significantly increased CH4 absorption, with significantly higher seasonal cumulative CH4 emissions (20.5%) in BM than PM, but significantly increased N2O emissions, with significantly lower seasonal cumulative N2O emissions (23.53%) in BM than PM. Both BM and PM improved garlic yield, with PM significantly increasing garlic yield by 18.86% compared to the CK. Moreover, film mulching significantly decreased the yield-scaled global warming potential (by 52.06% and 40.82% in PM and BM, respectively). PM had a significantly higher carbon footprint than BM. Film mulching improved NEEB by 9.29–11.78%. Considering crop yields and environmental benefits, we propose BM as an effective method for a green and efficient garlic production.

Soil Salt and Water Regulation in Saline Agriculture Based on Physical Measures with Model Analysis

Enhancing crop production in the saline regions of the Yellow River Delta (YRD), where shallow saline groundwater is prevalent, hinges on optimizing water and salt conditions in the root zone. This study explored the effects of various physical methods on soil water and salt dynamics during the cotton growing season in these saline areas. Three approaches were tested: plastic film mulching (FM), plastic film mulching with an added compacted soil layer (FM+CL), and ridge-furrow planting (RF). The HYDRUS-2D model (Version 3.02) was used to analyze changes in soil water and salt content in the root zone over time. The results showed that subsoil compaction significantly lowered salt build-up in the root zone, especially in the top 20 cm. Film mulching was crucial for reducing water loss in the Yellow River Delta. Crop transpiration increased by 7.0% under FM and 10.5% under FM+CL compared to RF planting. Additionally, FM+CL reduced soil salinity in the top 10 cm by 11.5% at cotton harvest time compared to FM alone. The study concludes that combining film mulching with a soil compaction layer is a promising strategy for local farmers, addressing soil water retention, salt management, and boosting cotton yields.

Optical properties of cotton and mulching film and feature bands selection in the 400 to 1120 nm range

Cotton is prone to being mixed with mulching film during the harvesting and packing process in China, which can significantly decrease the quality of cotton fiber and impact the quality of subsequent textile products. Mulching film is a translucent material that makes it challenging to detect optically, which presents an urgent challenge for cotton quality testing due to the lack of research on its detection mechanism. In this study, the absorption coefficient (μa), reduced scattering coefficient (μs'), and scattering anisotropy factor (g) of cotton lint and mulching film in the spectral region of 400–1120 nm were obtained using an integrating sphere-based spectroscopic measurement system. The accuracy of the system was validated using liquid phantoms made from polystyrene particles and absorbing dyes. The interval random frog (iRF) algorithm was used to select feature bands, and multivariate analysis of variance (MANOVA) was employed to assess the differences between cotton and mulching film in terms of μa and μs' in the selected feature bands. Support vector machine (SVM) and linear discriminant analysis (LDA) were utilized to classify the concatenation of μa, μs', μa and μs' of cotton and mulching film in full wavelengths and feature bands. The results demonstrated that there were significant differences in μa, μs' and g between cotton and mulching film due to their different chemical compositions, surface morphology, and tensile properties. The selected feature bands were in the blue band (456.1 nm), red bands (605.8 nm, 628.9 nm, 685.6 nm), and near-infrared bands (805.4 nm, 837 nm, 1006.3 nm). The SVM classification results of the concatenation of μa, μs', μa and μs' for cotton and mulching film were 100 % in both full wavelengths and feature bands. This study makes a significant contribution by reporting the optical properties of cotton and mulching film, which were used to determine the detection sensitive bands. These findings will serve as a theoretical foundation and data set for the development of detection equipment to identify mulching film in the cotton production process.

Enhancing Faba Bean Yields in Alpine Agricultural Regions: The Impact of Plastic Film Mulching and Phosphorus Fertilization on Soil Dynamics

Plastic film mulching is widely used in water and temperature-limited regions to enhance crop yields. Phosphorus (P) fertilization can address deficiencies in soil P availability. In this four-year field experiment conducted in an alpine agricultural area, we explored the effects of nitrogen (N) and P supply imbalance on faba bean cultivation, particularly examining intensified N competition between soil microbes and plants. The randomized block design comprised three film mulching treatments—no film mulching with flat planting (NMF), double ridges and furrows mulched with one plastic film (DRM), and three ridges and furrows mulched with one plastic film (TRM)—and three P levels—P0 (0 kg P ha−1), P1 (9.10 kg P ha−1), and P2 (18.2 kg P ha−1). The results indicated that NMF enhanced soil available N and microbial biomass N (MBN) during early growth stages, consequently improving faba bean yield, nodule weight, total N, and microbial biomass carbon (MBC) compared to DRM and TRM. DRM and TRM exhibited higher soil available N and MBN during later growth stages and higher soil temperature and water content, soil water storage (SWS), soil organic C (SOC), and soil C/N ratio than NMF. In NMF and DRM, P fertilization increased grain yield, nodule weight, SOC, total N, soil C/N ratio, soil available N, and MBC but decreased MBN during early growth stages, and decreased soil water content and SWS. TRM exhibited the opposite trend. P fertilization increased soil total P and available P. Overall, NMF combined with P fertilization (~18.2 kg P ha−1) significantly improved faba bean yield. However, it may also accelerate SOC decomposition, highlighting the need to consider N fertilizer application in this alpine agricultural region.

Effects of inorganic mulching on soil hydrothermal environment and tomato characters in the presence of unheated greenhouse cultivation

The present study was conducted by cultivating tomato (Solanum lycopersicum ‘Provence’) using varied inorganic mulching to investigate soil hydrothermal environment and tomato characters under unheated greenhouse cultivation in the cold zone of China. A total of 6 different treatments were adopted: no mulching (control), white film mulching (white film), black film mulching (black film), the white film with hole mulching (white hole), the black film with hole mulching (black hole), and snake skin bag mulching (snake skin). Inorganic mulching treatment significantly improved soil temperature and moisture, water use efficiency, tomato yield, and reduced soil water consumption. There was no significant difference observed in the variation of daily mean soil temperature between different mulching treatments, and the variation was in the range of 1.95–2.20 °C, which was significantly lower compared with the control (3.42 °C). The daily mean soil moisture varied significantly after different mulching treatments, with the highest level achieved by snake skin (23.37%), followed by black hole (22.55%), white hole (22.08%), white film (21.48%), black film (20.12%), and control (18.78%) in descending order. According to the research results, plastic-hole mulching, which include white hole and black hole treatments, performed better in maintaining soil temperature and moisture.

Co-exposure of butyl benzyl phthalate and TiO2 nanomaterials (anatase) in Metaphire guillelmi: Gut health implications by transcriptomics

During the COVID-19 pandemic, an abundance of plastic face masks has been consumed and disposed of in the environment. In addition, substantial amounts of plastic mulch film have been used in intensive agriculture with low recovery. Butyl benzyl phthalate (BBP) and TiO2 nanomaterials (nTiO2) are widely applied in plastic products, leading to the inevitable release of BBP and nTiO2 into the soil system. However, the impact of co-exposure of BBP and nTiO2 at low concentrations on earthworms remains understudied. In the present study, transcriptomics was applied to reveal the effects of individual BBP and nTiO2 exposures at a concentration of 1 mg kg−1, along with the combined exposure of BBP and nTiO2 (1 mg kg−1 BBP + 1 mg kg−1 nTiO2 (anatase)) on Metaphire guillelmi. The result showed that BBP and nTiO2 exposures have the potential to induce neurodegeneration through glutamate accumulation, tau protein, and oxidative stress in the endoplasmic reticulum and mitochondria, as well as metabolism dysfunction. The present study contributes to our understanding of the toxic mechanisms of emerging contaminants at environmentally relevant levels and prompts consideration of the management of BBP and nTiO2 within the soil ecosystems.