The objective of this study was to determine the movement patterns, heart rate recordings and work-to-rest ratios for different refereeing panels of South African Rugby Union referees during match-refereeing. The referees were monitored during play by means of a video camera in a total of 16 matches within a tournament. The frequency and duration of the different movement patterns were analysed using different functions of the Dartfish 5.5 TeamPro software package. Heart rates (HR) were recorded by a Suunto Team pack HR monitor system to determine the intensity of the movement patterns. The mean frequency of movement activities revealed a moderate practical significant difference between referee panels for jogging (d=0.51) and the mean duration indicated a moderate practical significant difference for jogging (d=0.43) and sprinting (d=0.43). The intensity of movement activities indicated a large practical significant difference for the anaerobic threshold (d=3.68) and sub-threshold (d=1.36) heart rate zones. A work-to-rest ratio of 1:4 was revealed for both referee panels during match-refereeing. The study revealed that contender panel referees completed more jogging activities and spent more time on jogging and sprinting activities than the provincial referees, and regarding intensities the contender panel referees also spent more time in low intensity heart rate zones.