Substrate Movement Research Articles

Precise measurements of chromatin diffusion dynamics by modeling using Gaussian processes

The spatiotemporal organization of chromatin influences many nuclear processes: from chromosome segregation to transcriptional regulation. To get a deeper understanding of these processes, it is essential to go beyond static viewpoints of chromosome structures, to accurately characterize chromatin’s diffusion properties. We present GP-FBM: a computational framework based on Gaussian processes and fractional Brownian motion to extract diffusion properties from stochastic trajectories of labeled chromatin loci. GP-FBM uses higher-order temporal correlations present in the data, therefore, outperforming existing methods. Furthermore, GP-FBM allows to interpolate incomplete trajectories and account for substrate movement when two or more particles are present. Using our method, we show that average chromatin diffusion properties are surprisingly similar in interphase and mitosis in mouse embryonic stem cells. We observe surprising heterogeneity in local chromatin dynamics, correlating with potential regulatory activity. We also present GP-Tool, a user-friendly graphical interface to facilitate usage of GP-FBM by the research community.

Durability of a New Thermal Aerogel-Based Rendering System under Distinct Accelerated Aging Conditions.

The widespread application of innovative thermal enhanced façade solutions requires an adequate durability evaluation. The present work intends to assess the durability of a new aerogel cement-based rendering system through the adaptation of different accelerated aging cycles, such as heating–freezing, freeze–thawing, and heat–cold. Several mechanical properties and also capillary and liquid water absorptions were tested for uncoated and coated specimens. A decrease in the mechanical strength, especially after freeze–thaw cycles, was observed. However, the water action promoted the late hydration of the cement paste contributing to the densification of the matrix and, consequently, the increase of the adhesive strength. Additionally, a decrease in the dynamic modulus of elasticity and an increase in the Poisson’s ratio were observed after aging, which indicates a higher capacity of the render to adapt to substrate movements, contributing to a reduction of cracking.

Testicular disposition of clofarabine in rats is dependent on equilibrative nucleoside transporters.

Acute lymphoblastic leukemia (ALL) is the most common cancer in children and adolescents. Although the 5‐year survival rate is high, some patients respond poorly to chemotherapy or have recurrence in locations such as the testis. The blood–testis barrier (BTB) can prevent complete eradication by limiting chemotherapeutic access and lead to testicular relapse unless a chemotherapeutic is a substrate of drug transporters present at this barrier. Equilibrative nucleoside transporter (ENT) 1 and ENT2 facilitate the movement of substrates across the BTB. Clofarabine is a nucleoside analog used to treat relapsed or refractory ALL. This study investigated the role of ENTs in the testicular disposition of clofarabine. Pharmacological inhibition of the ENTs by 6‐nitrobenzylthioinosine (NBMPR) was used to determine ENT contribution to clofarabine transport in primary rat Sertoli cells, in human Sertoli cells, and across the rat BTB. The presence of NBMPR decreased clofarabine uptake by 40% in primary rat Sertoli cells (p = .0329) and by 53% in a human Sertoli cell line (p = .0899). Rats treated with 10 mg/kg intraperitoneal (IP) injection of the NBMPR prodrug, 6‐nitrobenzylthioinosine 5′‐monophosphate (NBMPR‐P), or vehicle, followed by an intravenous (IV) bolus 10 mg/kg dose of clofarabine, showed a trend toward a lower testis concentration of clofarabine than vehicle (1.81 ± 0.59 vs. 2.65 ± 0.92 ng/mg tissue; p = .1160). This suggests that ENTs could be important for clofarabine disposition. Clofarabine may be capable of crossing the human BTB, and its potential use as a first‐line treatment to avoid testicular relapse should be considered.

Transfer of stabilising mutations between different secondary active transporter families.

Integral membrane transporters play essential roles in the movement of substrates across biological membranes. One approach to produce transporters suitable for structural studies is to introduce mutations that reduce conformational flexibility and increase stability. However, it can be difficult to predict which mutations will result in a more stable protein. Previously, we stabilised the uric acid‐xanthine transporter, UapA, a member of the SLC23 family, through introduction of a single‐point mutation, G411V, trapping the protein in the inward‐facing conformation. Here, we attempted to stabilise the structurally related BOR1 transporter from Arabidopsis thaliana, a member of the SLC4 family, by introducing the equivalent substitution. We identified possible residues, P362 and M363, in AtBOR1, likely to be equivalent to the G411 of UapA, and generated four mutants, P362V or L and M363F or Y. Stability analysis using heated Fluorescent Size Exclusion Chromatography indicated that the M363F/Y mutants were more stable than the WT AtBOR1 and P362V/L mutants. Furthermore, functional complementation analysis revealed that the M363F/Y mutants exhibited reduced transport activity compared to the P362V/L and WT proteins. Purification and crystallisation of the M363F/Y proteins yielded crystals that diffracted better than WT (5.5 vs 7 Å). We hypothesise that the increased bulk of the F and Y substitutions limits the ability of the protein to undergo the conformational rearrangements associated with transport. These proteins represent a basis for future studies on AtBOR1.

Protecting P-type plasma membrane H+-ATPases from ROS.

P-type ATPase are ubiquitous transport proteins across all kingdoms of life. These proteins share a common mechanism involving phosphorylation of an invariant aspartate to facilitate movement of substrates from protons to phospholipids across cellular membranes. In this issue of the Biochemical Journal, Welle et al. identify a conserved cysteine near the functionally critical aspartate of P-type plasma membrane H+-ATPases that protects the protein from reactive oxygen species.

Effect of a Summer Flood on Benthic Macroinvertebrates in a Medium-Sized, Temperate, Lowland River

Floods are naturally occurring extreme hydrological events that affect stream habitats and biota at multiple extents. Benthic macroinvertebrates (BM) are widely used to assess ecological status in rivers, but their resistance and resilience to floods in medium-sized, temperate, lowland rivers in Europe have not been sufficiently studied. In this study, we quantified the effect of a moderate (5-year return period) yet long-lasting and unpredictable flood that occurred in summer 2020 on the BM community of the Jeziorka River in central Poland. To better understand the mechanisms by which the studied flood affected the BM community, we also evaluated the dynamics of hydrological, hydraulic, channel morphology, and water quality conditions across the studied 1300 m long reach. Continuous water level monitoring, stream depth surveying, and discharge measurements. As well, in-situ and lab-based water quality measurements were carried out between March and August 2020. BM communities were sampled three times at eight sites along the reach, once before and twice after the flood. High flow velocities during the flood resulted in stream bed instability leading to sand substrate movement that caused streambed aggradation by up to 0.2 m. Dissolved oxygen and ammonium-nitrogen were major drivers of BM community structure. Taxa richness, abundance, and the BMWP-PL index declined significantly, whereas Shannon evenness and Simpson diversity indices showed no significant change in the first post-flood sampling, as indicated by Kruskal–Wallis and Tukey tests. Non-metric multidimensional scaling (NMDS) analysis showed that community composition was also significantly affected by the flood. Seven weeks after the flood peak (August 2020 sampling), BM communities had fully recovered from the disturbance. The results can serve as a first approximation of the resistance and resilience of BM communities for relevant applications in other medium-sized, low-gradient, temperate rivers.

MODELING OF BIOLOGICAL WASTEWATER TREATMENT ON THE BASIS OF QUICK-COMPUTING NUMERICAL MODEL

Purpose. The scientific paper involves the development of quick computing numerical model for prediction of output parameters of aeration tank. The numerical model may be used in predicting the effectiveness of aeration tank under different regimes of work. Methodology. To simulate the process of biological wastewater treatment in aeration tank numerical models were developed. The flow field in the aeration tank is simulated on the basis of potential flow model. 2-D transport equations are used to simulate substrate and sludge dispersion in the aeration tank. To simulate the process of biological treatment simplified model. For the numerical integration of transport equations implicit difference scheme was used. The difference scheme is built for splitting transport equations. Splitting of transport equation into two equations is carried out at differential level. The first equation of splitting takes into account the sludge or substrate movement along trajectories. The second splitting equation takes into account the diffusive process of substrate or sludge. To solve the splitting equations implicit difference scheme was used. For the numerical integration of potential flow equation the implicit scheme of conditional approximation was used. On the basis of constructed numerical model computer experiment was performed to investigate the process of biological treatment in aeration tank. Findings. Quick computing numerical model to simulate the process of biological treatment in the aeration tank was developed. The model can be used to obtain aeration tank parameters under different regimes of work. The developed model takes into account the geometrical form of the aeration tank. Originality. The numerical model which takes into account the geometrical form of aeration tank and fluid dynamics process was developed; the model takes into account substrate and sludge transport in aeration tank and process of biological treatment. Practical value. Efficient numerical model, so called «diagnostic models» was proposed for quick calculation of biological treatment process in aeration tank.

Fabrication of highly conducting ZnO/Ag/ZnO and AZO/Ag/AZO transparent conducting oxide layers using RF magnetron sputtering at room temperature

Sputter deposition of multilayer ZAZ (ZnO/Ag/ZnO) and AAA (AZO/Ag/AZO) TCO coatings has been done using a magnetron sputtering system. The deposition has been performed by moving the substrate under the desired target (ZnO or AZO and Ag) using a movable arm. This movable arm allows the substrate movement in the required order through a controller in a forward or reverse direction. Using this arrangement, the fabrication of ZAZ and AAA multilayer TCOs has been done on glass substrates at room temperature and the layer properties are found to be comparable with the properties of commercial ITO film. The fabricated ZAZ and AAA multilayers (with thickness around 70 nm) exhibit better electrical properties as compared to the commercial ITO film. The electrical resistivities of the ZAZ 2 and AAA 2 layers are 3.78 × 10−5 and 3.71 × 10−5 Ω cm, respectively which are much lower than the ITO resistivity of 4.56 × 10−4 Ω cm. The sheet resistances of ZAZ 2, AAA 2 and commercial ITO layers are 5.4, 5.3 and 16.8 Ω/□, respectively. The figure of merit (FoM) for AAA 2 multilayer is 45.5 × 10−3 Ω−1, much higher than commercial ITO film (23.7 × 10−3 Ω−1). The high-temperature annealing shows almost stable optical and electrical properties of the fabricated TCO layers up to 200 °C. Therefore, these TCOs can be used as electrodes for the solar cell fabrication where high-temperature annealing is required.

Density Functional Theory (DFT)-enhanced computational fluid dynamics modeling of substrate movement and chemical deposition process in spatial atomic layer deposition

Spatial atomic layer deposition (ALD) has been widely accepted as an effective strategy to improve the throughput in traditional ALD. This paper presents a Density Functional Theory (DFT)-enhanced Computational Fluid Dynamics (CFD) model, which includes substrate movement and a mechanistic chemical kinetics model derived from DFT in an inline spatial ALD. Substrate movement alters the steady flow field, but the disturbance does not damage the effective gas barrier built by N2 flow. Evaluation of deposition rate shows the growth per cycle (GPC) decreases as the substrate velocity increases. However, the growth per second (GPS) increases because of the considerable amount of time saved in faster substrate movements. Bottom and top purging designs are shown comparable in deposition rate. The DFT-enhanced CFD model provides a new route for accurate ALD modeling and simulation, and offers insightful information on reactor design and process optimization in spatial ALD.

The effect of sport and physical activity on transport proteins: implications for cancer prevention and control.

The present contribution briefly overviews the major biological functions of the plasma membrane and of the transport proteins (transporters), which enable the movement of different molecules and substrates (either charged or uncharged) by passive (facilitated diffusion) or active transport. In particular, transporters are overviewed at the level of the skeletal muscles, which represent a highly complex, heterogeneous, plastic and dynamic tissue and are one of the most abundant tissues in humans, accounting for up to 40% of their total weight and containing up to 50%-75% of all body proteins. Moreover, it is shown how sport and physical activity finely tune and modulate human proteome, especially in terms of structural and functional improvements concerning the density of the transport proteins. These changes are among the factors responsible for the positive outcomes of training, which involve mainly the cardiovascular and the endocrine/metabolic systems. Different kinds of training (strength and endurance) enable to achieve such improvements, even though there seems to exist a dose-relationship intensity-dependent effect, with responses after 6-8 weeks of exercise and disappearing in the chronic period (years of training). Finally, exercise-induced changes at the level of transporters can play a role in terms of cancer prevention and management. Regular physical activity and exercise can, indeed, counteract the side-effects of chemotherapy drugs, including doxorubicin and other anthracycline derivatives, which may impair the functions of cardiac and skeletal muscles, probably modulating the expression of multidrug resistance proteins.

Altered Hinge Conformations in APP Transmembrane Helix Mutants May Affect Enzyme-Substrate Interactions of γ-Secretase.

Cleavage of substrates by γ-secretase is an inherently slow process where substrate-enzyme affinities cannot be broken down into specific sequence requirements in contrast to soluble proteases. Nevertheless, despite its apparent sequence tolerance single point mutations in amyloid precursor protein can severely affect cleavage efficiencies and change product line preferences. We have determined by NMR spectroscopy the structures of the transmembrane domain of amyloid precursor protein in TFE/water and compared it to that of four mutants: two FAD mutants, V44M and I45T, and the two diglycine hinge mutants, G38L and G38P. In accordance with previous publications, the transmembrane domain is composed of two helical segments connected by the diglycine hinge. Mutations alter kink angles and structural flexibility. Furthermore, to our surprise, we observe different, but specific mutual orientations of N- and C-terminal helical segments in the four mutants compared to the wildtype. We speculate that the observed orientations for G38L, G38P, V44M, and I45T lead to unfavorable interactions with γ-secretase exosites during substrate movement to the enzyme's active site in presenilin and/or for the accommodation into the substrate-binding cavity of presenilin.

Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit

Activation of the Hedgehog pathway may have therapeutic value for improved bone healing, taste receptor cell regeneration, and alleviation of colitis or other conditions. Systemic pathway activation, however, may be detrimental, and agents amenable to tissue targeting for therapeutic application have been lacking. We have developed an agonist, a conformation-specific nanobody against the Hedgehog receptor Patched1 (PTCH1). This nanobody potently activates the Hedgehog pathway in vitro and in vivo by stabilizing an alternative conformation of a Patched1 "switch helix," as revealed by our cryogenic electron microscopy structure. Nanobody-binding likely traps Patched in one stage of its transport cycle, thus preventing substrate movement through the Patched1 sterol conduit. Unlike the native Hedgehog ligand, this nanobody does not require lipid modifications for its activity, facilitating mechanistic studies of Hedgehog pathway activation and the engineering of pathway activating agents for therapeutic use. Our conformation-selective nanobody approach may be generally applicable to the study of other PTCH1 homologs.

Proposed Mechanism of Action of Topically Applied Autologous Blood Clot Tissue.

Chronic wounds, especially in patients with diabetes, often represent clinical challenges. Recently, the use of a topically applied blood clot has garnered significant interest. This stromal matrix contains viable cells that are autologous, biocompatible, biological, and consistent with a metabolically active scaffold. It has been shown to be safe, effective, and cost-efficient. However, the mechanism of action of this modality remains elusive. We sought to identify a potential mechanism of action of an autologous blood clot. Review of clinical and scientific literature hypothesizes on how autologous blood clots may stimulate healing and facilitate the movement of critical substrates while lowering bioburden and fostering angiogenesis. Blood serves as a carrier for many components: red blood cells, white blood cells, platelets, proteins, clotting factors, minerals, electrolytes, and dissolved gasses. In response to tissue injury, the hemostatic mechanism uses a host of vascular and extravascular responses initiating primary, secondary, and tertiary hemostasis. The scaffold created by the autologous blood clot tissue provides a medium in which the body can transform the wound from a nonhealing chronic condition into a healing acute condition. The autologous blood clot tissue also creates a protective setting for the body to use its own mechanisms to promote wound healing in an organized manner. This transient scaffold recruits surrounding fibroblasts and promotes cell ingrowth to foster granulation tissue remodeling. Cells in this matrix sense not only soluble factors but also their physical environments. This well-orchestrated mechanism includes signals from soluble molecules, from the substrate/matrix to which the cell is adherent, from the mechanical or physical forces acting on it, and from contact with other cells. Topically applied autologous blood clot tissue can lower bacterial bioburden while stimulating angiogenesis and fostering the movement of keratinocytes and fibroblasts. Topically applied autologous blood clot tissue is a formidable cellular and tissue-based therapy that has been shown to be safe and effective. Although the central component of this therapy is blood, the autologous clot tissue creates a scaffold that performs as a biological delivery system that functions to control the release of growth factors and cytokines over several days.

Nanoliquid film flow due to a moving substrate and heat transfer

The present work analyzed the flow and heat transfer of the nanoliquid film flow over a moving inclined substrate. The motion of nanoparticles is induced by the action of both the gravitational force as well as the substrate movement. The hydrodynamic and thermal layers developing along the channel with a constant film thickness are resolved analytically. The corresponding pressure distribution, velocity field, temperature field and the physical quantities of wall shear stress as well as wall heat transfer rate are formulated in closed-form expressions. Seven different types of nanofluids are accounted for. Thermophysical properties of these particles enable us to visualize the flow and thermal development of the nanoliquid films in terms of the clean base fluid by the help of the defined shape factors. The considered mathematical model is validated against the available data in cases of special flow configurations. The carbon nanotubes are shown to have the highest heat transfer rates as compared to the other nanoparticles. On the other hand, the film thickness is much reduced and the wall shear is much amplified in the presence of silver nanoparticles.

Effect of the speed of the flat substrate movement on the air plasma jet transversal spreading at its impinging the surface

Low-temperature plasma jets are used to treat both the motionless and moving objects (targets or substrates). The gas-dynamic aspects of the plasma jet’s interaction with the target are important and have to be taken into account in selecting the most efficient plasma processing parameters. Clearly, the spreading of plasma jet over a fast-moving surface is significantly different from its spreading over a fixed target. This question is investigated by the example of the interaction of an air plasma jet with a surface of fast-rotating dielectric disk. It is shown the change in the shape of the jet striking perpendicularly the moving substrate happens due to the movement of a thin layer of the ambient air adjacent to the fast-moving surface. This effect can have a noticeable influence on the reactive particles transfer from the plasma jet onto the surface to be treated.

Estimation of vital population rates to assess the relative health of mussel assemblages in the Upper Mississippi River

Abstract Native freshwater mussels are a guild of benthic, filter feeding invertebrates that perform important ecological functions in rivers. Because of their long lifespans (30–50 years or longer), mussels are slow to respond to human‐induced alterations. Thus, development of sensitive indicators of mussel population responses to river conditions and management would be beneficial. Compared to marine species, estimation of vital rates (e.g. survival, growth) in freshwater mussels has received little attention. We placed passively integrated transponder tags on 578 mussels of four species (Amblema plicata, Cyclonaias pustulosa, Obliquaria reflexa, and Pleurobema sintoxia) in a well‐studied mussel assemblage in a side channel of the upper Mississippi River. Growth and survival of tagged mussels were assessed annually for 4 years across core (high density) and peripheral (low density) areas of the assemblage. Overall survival was highly variable, ranging from c. 15 to 90%, and was related to life history, habitat quality, and hydrologic events. Survival, which varied significantly among species and over time, was consistently higher in the dense and species‐rich core of the mussel assemblage, relative to the periphery because substrates were consistently more stable in the core of the mussel bed relative to the periphery. Substrate movement during low flows was an order of magnitude lower in the core relative to the periphery, and survival was inversely related to stability of river substrates. Patterns in habitat‐specific survival indicate source–sink population dynamics such that mussels in the core habitat provide recruitment to the periphery, but mussels in the periphery are subject to unsustainably low survival; additional studies to track the source of recruitment in the periphery are needed to test this hypothesis. Growth rate did not vary significantly between core and peripheral areas but did vary by species. Growth rate (proportional change per year) declined with age, and was similar at mean age for A. plicata (0.016 per year), P. sintoxia (0.015 per year), and C. pustulosa (0.013 per year), but much lower for O. reflexa (0.008 per year). Effective management decisions for mussels requires a better understanding of how vital rates govern populations and how they vary across a suite of physical and biological factors. Information on how population vital rates vary among species and over time gives managers another tool to understand how mussels may respond to management actions such as habitat restoration projects. Given the importance of substrate stability inferred from this study, management actions that maintain or increase substrate stability are likely to result in high quality mussel assemblages and may restore a valuable component of ecosystem function in this region.

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley.

Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2, HvTIP2;2, HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action.

Hrd1 forms the retrotranslocation pore regulated by auto-ubiquitination and binding of misfolded proteins.

During endoplasmic-reticulum-associated protein degradation (ERAD), misfolded proteins are polyubiquitinated, extracted from the ER membrane and degraded by the proteasome1-4. In a process called retrotranslocation, misfolded luminal proteins first need to traverse the ER membrane before ubiquitination can occur in the cytosol. It was suggested that the membrane-embedded ubiquitin ligase Hrd1 forms a retrotranslocation pore regulated by cycles of auto- and deubiquitination5-8. However, the mechanism by which auto-ubiquitination affects Hrd1 and allows polypeptides to cross the membrane and whether Hrd1 forms a membrane-spanning pore remained unknown. Here, using purified Hrd1 incorporated into different model membranes, we show that Hrd1 auto-ubiquitination leads to the opening of a pore. Substrate binding increases the pore size and its activity, whereas deubiquitination closes the pore and renders it unresponsive to substrate. We identify two binding sites for misfolded proteins in Hrd1, a low-affinity luminal site and a high-affinity cytoplasmic site formed following auto-ubiquitination of specific lysine residues in Hrd1's RING domain. We propose that the affinity difference between the luminal and cytoplasmic binding sites provides the initial driving force for substrate movement through Hrd1.

Pine Pitch Canker (PPC): Pathways of Pathogen Spread and Preventive Measures

Fusarium circinatum (Nirenberg and O’ Donnell) is the causal agent of pine pitch canker (PPC) disease, one of the most devastating forest diseases worldwide. Long-distance spread occurs mainly through the movement of infected seeds whereas at regional level, the movement of seedlings, substrates, or containers may play an important role in fungal dispersal. Invasion of nurseries takes place via infected seeds and further spread can occur by planting contaminated seedlings, especially due to the possibility of infected plants remaining symptomless. Once established, F. circinatum spreads by rain, wind, and insects. The natural spread of the pathogen is limited due to the short dispersal distances of the spores and the fairly short flight distances of disseminating insects. In this review, we summarize the currently known dispersal pathways of the pathogen, discussing both natural and human-assisted processes. With the purpose of understanding how to best intervene in the disease’s development in nurseries and forests, we outline the epidemiology of the pathogen describing the key factors influencing its spread. Preventive measures to control the spread of F. circinatum locally and globally are described with special emphasis on the challenges in implementing them.

Effect of Pouring Temperature on Microstructure and Properties of A356 Alloy Strip by a Novel Semisolid Micro Fused-Casting for Metal

A novel semisolid micro fused-casting (MFC) for preparing A356 alloy strips is proposed, and the effects of process parameters of pouring temperature on the microstructure and properties of A356 alloy strips are investigated. MFC means that the semisolid metal slurry was pressed out from the outlet of bottom of crucible to the movable plate, and directly solidified and formed layer by layer. The microstructure and properties of A356 semisolid alloy slurry were influenced by the cooling conditions. Results show that the aluminu alloy A356 strip samples fabricated by micro fused-casting had good performances and uniform structures with the pouring temperature at 595 °C and the substrate movement speed at 18 mm/s. The fine grains of the primary α-Al phase with average grain size of 53 μm and shape factor up to 0.72 was obtained, the ultimate tensile strength of the aluminum alloy A356 strip reaches 243.79±3.91 MPa, while the average vickers hardness is 82.65±1.86 HV.