Mouth Disease Virus Infection Research Articles

Susceptibility to foot and mouth disease virus infection in vaccinated cattle, and host BoLA A and BoLA DRB3 genes polymorphism.

The online version contains supplementary material available at 10.1007/s13337-021-00754-8.

Effect of Experimental Foot and Mouth Disease Virus Infection on the Activation and Polarization of Bovine Monocyte-Derived Macrophages 

Effect of Experimental Foot and Mouth Disease Virus Infection on the Activation and Polarization of Bovine Monocyte-Derived Macrophages 

Ribavirin as a curative and prophylactic agent against foot and mouth disease virus infection in C57BL/6 suckling and adult mice model.

The online version contains supplementary material available at 10.1007/s13337-021-00746-8.

Foot and Mouth Disease Virus Infection Seroprevalence Study in Dairy Cattle Reared by Smallholder Farmers in Welmera District, Central, Oromiya Ethiopia

Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals and one of the endemic diseases in Ethiopia. A study was conducted to determine foot and mouth disease virus seroprevalence and potential risk factors in welmera district, central Oromiya, Ethiopia. Purposive sampling was performed in the respective district and kebeles where there was recent history of outbreaks. A total of 126 sera samples were collected from randomly selected cattle and tested using ELISA for antibodies against nonstructural proteins of foot and mouth disease virus. The seroprevalence of foot and mouth disease virus infection in Welmera district was 49.2%. The result indicated that seropositivity of FMD infection FMD was higher in females (54%) than in males (46.1%) and this variation was statistically significant (χ2= 12.93, p = 0.001). The associations of seropositivity of FMD in relation to body condition score and management system were statistically significant (χ2= 5.34, p = 0.023, χ2= 6.7, p = 0.003) respectively. Age related seropositivity was not statistically significant (P>5%). Cross bred cattle were 2.17 (OR = 2.17, 95% CI: 2.10 – 5.74) times more likely to be infected by FMD than local breeds. This showed that FMD infection is widely prevalent in the district. Therefore, foot and mouth disease control will depend on identifying virus serotypes circulating in the area and regular vaccination program.

Seroprevalence of Foot and Mouth Disease Virus Infection in Some Wildlife and Cattle in Bauchi State, Nigeria.

Foot and mouth disease (FMD) is an important transboundary viral disease of both domestic and wild cloven-hoofed animals characterized by high morbidity with devastating consequence on the livestock worldwide. Despite the endemic nature of FMD in Nigeria, little is known about the epidemiology of the disease at the wildlife-livestock interface level. To address this gap, blood samples were collected between 2013 and 2015 from some wildlife and cattle, respectively, within and around the Yankari Game Reserve and Sumu Wildlife Park in Bauchi State, Nigeria. Wild animals were immobilized using a combination of etorphine hydrochloride (M99® Krüger-Med South Africa) at 0.5–2 mg/kg and azaperone (Stresnil®, Janssen Pharmaceuticals (Pty.) Ltd., South Africa) at 0.1 mg/kg using a Dan-Inject® rifle (Dan-Inject APS, Sellerup Skovvej, Denmark) fitted with a 3 ml dart syringe and for reversal, naltrexone (Trexonil® Kruger-Med South Africa) at 1.5 mg IM was used, and cattle were restrained by the owners for blood collection. Harvested sera from blood were screened for presence of antibodies against the foot and mouth disease virus (FMDV) using the PrioCHECK® 3ABC NSP ELISA kit, and positive samples were serotyped using solid-phase competitive ELISA, (IZSLER Brescia, Italy). Out of the 353 sera collected from cattle and wildlife 197 (65.7%) and 13 (24.5%) (P < 0.05), respectively, tested positive for antibodies to the highly conserved nonstructural 3ABC protein of FMDV by the FMDV-NS blocking ELISA. Classification of cattle into breed and sex showed that detectable antibodies to FMDV were higher (P < 0.05) in White Fulani 157 (72.8%) than in Red Bororo 23 (39.7%) and Sokoto Gudali 17 (33.3%) breeds of cattle, whereas in females, detectable FMDV antibodies were higher (P < 0.05) 150 (72.8%) than in males 47 (50.0%). In the wildlife species, antibodies to FMDV were detected in the waterbucks 2 (28.6%), elephant 1 (25.0%), wildebeests 4 (33.3%), and elands 6 (25.0%). Four serotypes of FMDV: O, A, SAT 1, and SAT 2 were detected from the 3ABC positive reactors in waterbucks, elephants, wildebeests, and elands. The results showed presence of antibodies to FMDV in some wildlife and cattle and suggested that wildlife could equally play an important role in the overall epidemiology of FMD in Nigeria. FMD surveillance system, control, and prevention program should be intensified in the study area.

Bioinformatics Analysis of Key micro-RNAs and mRNAs under the Hand, Foot, and Mouth Disease Virus Infection.

To clarify crucial key micro-RNAs and mRNAs associated with hand, foot, and mouth disease (HFMD) virus infection, we conducted this bioinformatics analysis from four GEO datasets. The following datasets were used for the analysis: GSE85829, GSE94551, GSE52780, and GSE45589. Differentially expressed genes (DEGs) were acquired, and the analysis of functional and pathway enrichment and the relative regulatory network were conducted. After screening common differentially expressed miRNAs (DE-miRNAs), five key miRNAs were acquired: miR-100-3p, miR-125a-3p, miR-1273g-3p, miR-5585-3p, and miR-671-5p. There were three common enriched GO terms between miRNA-derived prediction and mRNA-derived analysis: biosynthetic process, cytosol, and nucleoplasm. There was one common KEGG pathway, i.e., cell cycle shared between miRNA-based and mRNA-based enrichment. Using TarBase V8 in DIANA tools, we acquired 1,520 potential targets (mRNA) from the five key DE-miRNAs, among which the159 DE-mRNAs also included 11 DEGs. These common DEGs showed a PPI network mainly connected by SMC1A, SMARCC1, SF3B3, LIG1, and BRMS1L. Together, changes in five key miRNAs and 11 key mRNAs may play crucial roles in HFMD progression. A combination of these roles may benefit the early diagnosis and treatment of HFMD.

Kinetics of Interferon gamma and Interleukin-21 response following foot and mouth disease virus infection

Foot and mouth disease (FMD) is one of the most contagious diseases of cloven footed animals causing significant economic impediment in livestock production system. The immune response to FMD virus (FMDV) infection is regulated by a complex interplay between various cells, cytokines and other immune components. Based on the well established role of Interferon-gamma (IFN-γ) and Interleukin-21 (IL-21) in viral infections, this study aimed to determine expression level of these cytokines in clinically infected adults and calves; and the results were compared with those in the subclinically infected animals up to 120 days post outbreak (DPO) in a vaccinated cattle herd. The expression level of IFN-γ and IL-21 was assayed on 0, 7, 14, 28, 60, 90, and 120 DPO by enzyme linked immunosorbent assay (ELISA) with simultaneous assessment of FMDV structural protein-antibody titer against serotype ‘O’ by liquid phase blocking ELISA (LPBE) and nonstructural protein-antibody, a differential marker of infection, using r3AB3 indirect ELISA (r3AB3 I-ELISA). Although, the peak expression of IFN-γ was observed on 14 DPO across all categories of animals, the clinically infected animals registered a significant increase in IFN-γ level as compared to the subclinically infected population possibly due to the difference in the extent of virus replication and inflammation. The IL-21 level increased significantly during 14˗28 DPO and highest expression was noticed on 28 DPO. The increase in the expression level of IFN-γ and IL-21 at 28 DPO correlated with the increase in antibody titer as determined by LPBE suggesting the role of these cytokines in augmenting immune response to FMDV infection.

Sero Survey of Foot and Mouth Disease Virus Infection in Cattle Crossing Some Major Border States in Northwestern Nigeria

Abstract Foot and mouth disease (FMD) poses a major constraint to international trade in animals and animal products in sub-Saharan Africa. A retrospective and serological survey was conducted in two major Border States of Sokoto and Kebbi in north-western Nigeria. This study was aimed at determining the sero-prevalence of FMD virus (FMDV) antibodies in cattle at international animal control posts and to examine cattle population movement across the border area for a period of one year (January to December 2014) from the available records. Eight hundred and eighty (880) sera samples were collected and screened for the presence of antibodies to FMDV using the competitive enzyme linked immunosorbent assay (ELISA) technique. The data were subjected to chi-square and relative risk to check for independence and association. An overall seropositive rate was found to be 55.2 % (486/880). A 79.9 % (359/450) sero-positive rate was obtained from the Kamba border, while 29.5 % (127/430) was found at the Illela border. Kamba showed a statistically significant (P &lt; 0.05) higher sero-prevalence when compared with cattle that are crossing the Illela border (Relative Risk 2.70; 95 % Confidence Interval 2.317—3.149). Retrospective data from the control posts revealed that an average number of 2019 and 2747 of cattle, respectively, crossed the Kamba and Illela international borders monthly. The highest influx of animals from the Niger Republic through the Illela international border was encountered between the month of March and April 2014. The magnitude of the presence of FMDV Non-structural protein (NSP) antibodies in the study areas is an indication of the infection and the presence of the virus in the study areas and the neighbouring countries.

Epidemiological Insights of Foot and Mouth Disease Virus Infection among Cattle and Buffaloes in Sharkia Governorate, Egypt

Epidemiological Insights of Foot and Mouth Disease Virus Infection among Cattle and Buffaloes in Sharkia Governorate, Egypt

Epidemiological Insights of Foot and Mouth Disease Virus Infection among Cattle and Buffaloes in Sharkia Governorate, Egypt

Foot-and-mouth disease (FMD) is endemic in Egypt and in most parts of Africa causing huge economic losses. Control of FMD using vaccination requires information on the occurrence of various FMDV serotypes. This study aimed to determine the prevalence of FMDV serotypes in Sharkia Governorate, Egypt. A total number of 643 different samples, within ten different localities, were collected from both cattle and buffaloes (n = 283) of different, age, sex, immune status against FMD, and health status. Field samples (n = 360) have been screened for FMDV by RT-PCR using universal primers and were further subtyped using serotype-specific primers. Additionally, serum samples (n = 283) have been analyzed by applying FMDV serotype-specific antibody ELISA. The RT-PCR screening revealed that a total number of 39/283 (13.8%), 61/283 (21.6%) and 17/38 (44.7%) animals were positive for FMDV serotype O, A and SAT2, respectively. While, by ELISA, neutralizing antibodies directed against FMDV serotype O, A, and SAT2, were found in 177/283 (62.5%), 171/283 (60.4%) and 27/38 (71.1%) serum samples, respectively. These results indicated the endemic status of the FMDV serotypes O, A and SAT2 in Sharkia Governorate despite routine FMD vaccination programs. Although many variations of disease prevalence were recorded between animals of different, age, sex and immune and health status but it was obvious that FMD was more prominent and prevalent in buffaloes (47.1%) than in cattle (34.1%). Therefore, control efforts should focus on reducing the circulation of FMDV among susceptible livestock with special attention towards water buffaloes. Continuous surveillance, at molecular and immunological levels, of FMDV serotypes is needed for the effectiveness of any adopted control strategy targeting FMD including vaccination.

Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

Seroprevalence of foot and mouth disease virus infection in pigs from Zuru, Nigeria.

Aim:This study was conducted to determine the seroprevalence and distribution of foot and mouth disease virus (FMDV) infection in pigs from Zuru, Kebbi State, Nigeria.Materials and Methods:Cross-sectional serological surveys were conducted between May and December 2013 using the immunochromatography assay technique. Structured questionnaires were administered to households identified at pig rearing areas to obtain the population structures and some information on managemental practices.Results:A total number of 849 pigs were enumerated at 37 pigs rearing households. Tudun wada had the largest concentration of pigs (237 pigs), while Dabai has the least (38 pigs). A total of 250 blood samples were collected, of which 45 (18%) were positive; Zango has the highest seroprevalence (1.6%), while Dabai recorded the least (0.4%). Based on sex and age, the infection was higher in female (10.4%) and young pigs (11.6%) than male (7.6%) and adult pigs (6.4%), respectively. There was no significant (p>0.05) association between infection and pig rearing areas, sex, and age. Furthermore, none of the 250 pigs examined for classical FMDV lesions was positive.Conclusion:The results of this study showed that FMD is an important disease of pigs in the study areas. This result justifies the need for more attention and subsequent molecular study to identify the circulating FMDV in the area, which will help in the implementation of effective control measures.

Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.

Indirect ELISA using recombinant nonstructural protein 3D to detect foot and mouth disease virus infection associated antibodies

Foot-and-mouth disease (FMD) is a highly infectious disease of transboundary importance. Routine biannual vaccination along with surveillance activities is seen as the principal to control FMD in India. Non-structural protein (NSP) based immunoassays are the test of choice for the differentiation between infected and vaccinated population. In this study, 3D protein of FMD virus was expressed in Escherichia coli and an indirect ELISA (I-ELISA) was developed to detect 3D-antibodies in the infected bovines. 3D I-ELISA demonstrated comparable diagnostic sensitivity (97.6%) but lower specificity (80.8%) as compared to the in-house r3AB3 I-ELISA. However, the specificity values varied significantly for naïve and vaccinated samples and were observed to be 98.42% and 76.93%, respectively. A moderate degree of concordance (88.5%) was observed between the overall results of two ELISAs. 3D I-ELISA displayed a considerably lower specificity in uninfected vaccinated samples, thereby suggesting against its application for serosurveillance in intensively vaccinated population. However by virtue of its high diagnostic sensitivity and longer duration of persistence of 3D-antibody post-infection, 3D I-ELISA could be adopted for seroepidemiological investigations in regions not practicing vaccination and could be extended to susceptible species which are generally not covered by vaccination.

Hematological Variations Associated with Bovine Foot and Mouth Disease Virus Infection

Hematological analysis of bovine blood was carried out to establish possible variations associated with foot and mouth disease (FMD) infection. Random sampling of FMD infected bovine blood from the reported outbreaks in Bode, Bode II and Buakaru-rotuwa wards in ilesha baruba, Baruten LGA in kwara state was collected forth nightly in December, 2010, January, February, and March, 2011. Serum antibodies detection using NS-ELISA revealed out of the ninety cattle sampled in the fifteen outbreaks, 64 showed positive evidence of FMD with percentage distribution of 37.5% and 62.5% for bulls and cows respectively. This was significant (p 0.05) association with FMD sero-positive cattle by chi square analysis. Therefore, this finding provides preliminary information on hematological variation observed during FMD outbreak. Further experimental case-control study on the hematology of FMD infected cattle is thus suggested.

Selection of Highly Susceptible Cell Lines to Foot and Mouth Disease Virus Infection

Different non-established cultures were examined to find those that showed high sensitivity to Foot and Mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The level of detection in this system was up to ten times higher than in BHK21 cells, which were commonly used for FMDV isolation and production. Viral production levels in ovine kidney cultures ranged from similar to twice as high as in BHK21. Ovine kidney cultures maintained these characteristics for at least 18 passages, allowing their use as an alternative system for FMDV diagnoses.

Antibody response against three widespread bovine viruses is not impaired in Holstein cattle carrying bovine leukocyte antigen DRB3.2 alleles associated with bovine leukemia virus resistance

Due to the wide dissemination of bovine leukemia virus (BLV) infection among dairy cattle, control and eradication programs based on serological detection of infected cattle and subsequent culling face a major economic task. In Argentina, genetic selection of cattle carrying alleles of the bovine leukocyte antigen (BoLA) DRB3.2 gene associated with BLV-infection resistance, like *0902, emerges as the best additional tool toward controlling virus spread. A potential risk in expanding or segregating BoLA selected populations of cattle is that it might increase susceptibility to other common viruses. Special concern raises the strong association found between low proviral load and low antibody titer against major BLV structural proteins. This phenomenon might depend on host genetic factors influencing other viruses requiring, unlike BLV, strong and long-lasting humoral immune response to prevent infection. In this study, we demonstrate that there is no association among neutralizing antibody titers against foot and mouth disease virus, bovine viral diarrhea virus, or bovine herpesvirus type 1 and polymorphism of the BoLA DRB3.2 gene. Conversely, there is strong association between BoLA DRB3.2*0902 and low antibody titers against 2 BLV structural proteins—env gp51 and gag p24—to date, the best BLV resistance marker. There is also significant association between low antibody titers against gp51 and p24 and BoLA DRB3.2*1701 and low antibody titers against p24 and BoLA DRB3.2*1101 or 02. Our data suggest that increasing BoLA-selected BLV-resistant cattle or segregating BoLA-associated alleles to BLV susceptibility would not affect the resistance or the predisposition to bovine viral diarrhea virus, bovine herpesvirus type 1, or foot and mouth disease virus infection.

Recombinant fusion protein and DNA vaccines against foot and mouth disease virus infection in guinea pig and swine.

In this study, we provide evidence that a recombinant fusion protein containing beta-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.