This study aims to 1) describe mean changes in muscular and motor fitness components in sibships over 2 yr, 2) analyze individual tracking of fitness within sibships, 3) investigate sibling resemblance in fitness over time, and 4) examine the joint influence of biological, behavioral, and familial characteristics on fitness. The sample comprises 166 Portuguese biological sibling pairs (brother-brother, sister-sister, brother-sister) age 9-17 yr assessed at baseline and 2 yr later. Physical fitness components were measured with standardized tests. Percentage body fat and biological maturation were assessed, and physical activity, diet, screen time, and familial characteristics were obtained by questionnaires. Multilevel models were used to analyze the clustered longitudinal data. Crude results showed that, on average, all sib-types increased their muscular and motor fitness components from baseline to follow-up (except sister-sister pairs in standing long jump and shuttle run). When adjusted for covariates, the mean changes were no longer significant. Individual tracking was moderate to high for the muscular component but low to moderate for the motor component. Consistency in sibling resemblance was higher in sister-sister pairs than in brother-brother and brother-sister pairs. More mature sibs were fitter in both components, whereas siblings with higher body fat percentage were less fit. Screen time, physical activity, and parental occupation were not associated with fitness components. Biological characteristics were more strongly associated with fitness components than individual behaviors and familial characteristics. Furthermore, the muscular component tracked better than the motor component. Sister-sister pairs had greater resemblance in fitness over time compared with brother-brother or sister-brother pairs.
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