Bioanalytical techniques play an important role in monitoring the effects of environmental stress factors on fundamental life processes. In terms of space flight and extraterrestrial research, radiation, altered and microgravity are known to induce changes in gene expression. We report the use of an on-line transverse shear mode (TSM) acoustic wave biosensor to detect the initiation of gene transcription and DNA — drug binding. Since this biosensor offers real-time, label free monitoring of biological processes, it is possible to detect sequential binding steps as demonstrated in this paper. Furthermore, this sensor responds to several factors in the liquid phase such as viscosity, elasticity, surface tension, charge distribution and mass loading, which can in turn be influenced by specific gravity. The sensing device is a piezoelectric quartz crystal onto which the probe molecule (DNA in this case) is immobilized. Change in resonance frequency of the crystal in response to the binding of the target molecule(s), RNA polymerase and actinomycin-D, is fit to an equivalent circuit model from which multidimensional data is extracted. By performing multivariate analysis on this data we are able to observe interactions between several of these data series representing parameters such as motional resistance and capacitance. As well, we are able to observe the dominating parameters (for instance, frequency vs. motional resistance, which in turn can correspond to mass loading vs. energy dissipation) during the course of the experiment, as they vary between the different steps. Such advantages offered by the TSM sensor along with multivariate analysis are indispensable for biotechnological work under the influence of microgravity as several variables come into play.