Morphometric Traits Research Articles

Principal component analysis of body biometric traits in Marathwadi buffaloes

The identification of livestock breed is a necessity for its long-term maintenance and utilisation. Principal component analysis of morphometric traits has proved successful for reduction in the number of features needed for morphological evaluation in livestock species, which keeps costs down and saves time and efforts. Eighteen body biometric traits, viz. Height at withers, Leg length, Neck length, Neck circumference, Body length, Chest girth, Abdominal girth, Face length, Face width, Ear length, Horn length, Horn base circumference, Distance between horns, Hip-bone distance, Pin-bone distance, Distance between hip and Pubis bone, Rump length and Tail length of 103 Marathwadi buffaloes were analysed by using Promax rotated PCA with Kaiser Normalization to explain body conformation. Highest correlation was observed between HW × LEG (0.77), KMO Measure of Sampling Adequacy was 0.794 while Bartlett’s test of Sphericity was significant with chi-square value of 640.494. PCA revealed five components which explained about 61.91% of the total variation. First component explained 31.05% describing general body conformation with highest loadings for BH, CG, LEG and HB. The communality ranged from 0.43 (HC) to 0.78 (FW). Total variance explained by second, third, fourth and fifth component was 10.83%, 7.34%, 6.75% and 5.92% respectively. The rotated pattern matrix showed higher loadings of NC, PG, FL for Marathwadi buffaloes. Traits having high loadings in pattern matrix had high correlation with the components under structure matrix. Present study suggested that PCA can successfully reduce the dimensionality and first PC can be used in the evaluation and comparison of body conformation in Marathwadi buffaloes.

Effect of genotype on hatchability, growth, morphometric and carcass traits of Chicken

Effect of genotype on hatchability, growth, morphometric and carcass traits of Chicken

Sexual size and shape dimorphism, and allometric scaling in the pupal and adult traits of Eristalis tenax.

The patterns and amount of variation in size, shape, and/or life history traits between females and males are fundamentally important to gain the comprehensive understanding of the evolution of phenotypic diversity. In addition, the covariation of phenotypic traits can significantly contribute to morphological diversification and sexual dimorphism (SD). Using linear and geometric morphometrics, 237 Eristalis tenax specimens sampled from five populations were, therefore, comparatively assessed for the variation in sexual size dimorphism (SSD), sexual shape dimorphism (SShD), and life history traits, as well as for trait covariation (ontogenetic and static allometry). Pupal body, adult wing, and body mass traits were analyzed. Female-biased SSD was observed for pupal length, width, and centroid size, adult wing centroid size, mass, wing loading, and wing area. Conversely, pupal length/width ratio, developmental time, and mass were not found to be sexually dimorphic. Next, wing SShD, but not pupal body SShD was revealed, while allometry was found to be an important "determinant of SD" at the adult stage, with only a minor impact at the pupal stage. By comparing the patterns of covariance (based on allometric slope and intercept) between respective body mass and morphometric traits of pupae and adults, greater variation in allometric slopes was found in adult traits, while static allometries of the two stages significantly differed, as well. Finally, the results indicate that changes in the allometric intercept could be an important source of intraspecific variation and SD in drone fly adults.

MORPHOLOGICAL CHARACTERISTICS OF THE CARP (CYPRINUS CARPIO) FROM THE WESTERN PART OF LAKE BALKASH UNDER CONDITIONS OF MODERN ANTHROPOGENIC PESSURE

Balkash Lake is one of the largest reservoirs in South-Eastern Kazakhstan, taking the 2nd place in terms of area after the Caspian Sea. The current environmental problems are damaging the ichthyofauna of the lake, where many populations of commercial fish species are significantly affected. Fish, being part of an ecosystem, are able to show adaptive properties by changing their biological (species) characteristics in response to environmental changes. With the increase in pollution of aquatic ecosystems, qualitative changes in the external and internal structure of fish occur. The chosen object of study, carp (Cyprinus carpio), is a good example of manifestations of adaptations in response to changes in the quality of the habitat. The study of the variability of morphometric traits in fish is an important direction in identifying anomalies and monitoring the general health of fish. In this regard, we studied 28 plastic features and 16 meristic (countable) features, as well as the presence of deviations in the appearance of the carp (external malformations). The study using the method of multivariate statistical analysis made it possible to determine the load and direction of changes in individual signs of carp. Based on the results of morphological studies, the phenotypic plasticity of carp correlates with the negative environmental conditions of the western part of Lake Balkash.

Morphological variability and genetic potential of henna (Lawsonia inermis) germplasms in semi-arid regions of Rajasthan

Henna (Lawsonia inermis L.) is a commercial and export-oriented important perennial shrub used in the ornamentation of palm, feet, dyeing of hair, etc. Developing high-yielding varieties and elite plant materials are the best method to increase the productivity and quality in henna. An experiment was conducted at research farm of ICAR-Central Arid Zone Research Institute, Regional Research Station, Pali-Marwar, Rajasthan during 2018–22 in Randomized block design to assess variability among morphometric traits and genetic parameters for dry leaf yield and attributing characters in Henna. The results indicated that among 19 germplasms maximum dry leaf yield and minimum number of inflorescence was recorded in CZ-RSPH-8 followed by CZ-RSPH-15. The accession CZ-RSPH-9 (2.33%) exhibited the highest lawsone content (%) followed by CZ-RSPH-8 and CZ-RSPH-15. The estimates of phenotypic co-efficient variation (PCV) were higher than the respective genotypic co-efficient variation (GCV). The number of inflorescences registered high heritability with high genetic advance. Path analysis exhibited the direct effect of the number of inflorescence with dry leaf yield. Cluster analysis revealed two major clusters classified into 6 and 13 germplasm which depicts the diverse degree of relatedness among different accessions.

CRISPR/Cas9 editing of wheat Ppd-1 gene homoeologs alters spike architecture and grain morphometric traits.

Mutations in Photoperiod-1 (Ppd-1) gene are known to modify flowering time and yield in wheat. We cloned TaPpd-1 from wheat and found high similarity among the three homoeologs of TaPpd-1. To clarify the characteristics of TaPpd-1 homoeologs in different photoperiod conditions for inflorescence architecture and yield, we used CRISPR/Cas9 system to generate Tappd-1 mutant plants by simultaneous modification of the three homoeologs of wheat Ppd-1. Tappd-1 mutant plants showed no off-target mutations. Four T0-edited lines under short-day length and three lines under long-day length conditions with the mutation frequency of 25% and 21%, respectively. These putative transgenic plants of all the lines were self-fertilized and generated T1 and T2 progenies and were evaluated by phenotypic and expression analysis. Results demonstrated that simultaneously edited TaPpd-1- A1, B1, and D1 homoeologs gene copies in T2_SDL-8-4, T2_SDL-4-5, T2_SDL-3-9, and T2_LDL-10-9 showed similar spike inflorescence, flowering time, and significantly increase in 1000-grain weight, grain area, grain width, grain length, plant height, and spikelets per spike due to mutation in both alleles of Ppd-B1 and Ppd-D1 homoeologs but only spike length was decreased in T2_SDL-8-4, T2_SDL-4-5, and T2_LDL-13-3 mutant lines due to mutation in both alleles of Ppd-A1 homoeolog under both conditions. Our results indicate that all TaPpd1 gene homoeologs influence wheat spike development by affecting both late flowering and earlier flowering but single mutant TaPpd-A1 homoeolog affect lowest as compared to the combination with double mutants of TaPpd-B1 and TaPpd-D1, TaPpd-A1 and TaPpd-B1, and TaPpd-A1 and TaPpd-D1 homoeologs for yield enhancement. Our findings further raised the idea that the relative expression of the various genomic copies of TaPpd-1 homoeologs may have an impact on the spike inflorescence architecture and grain morphometric features in wheat cultivars.

Genetic association analysis of rs662799 ( − 1131A > G) polymorphism of APOA5 gene with morphometric and physio-metric traits using multiplex PCR

BackgroundThe apolipoprotein A5 (APOA5) gene, significantly expressed in liver, has been involved in regulation of triglyceride metabolism, plasma lipid levels, serum adipokine levels and cardiovascular traits. A single-nucleotide polymorphism rs662799 ( − 1131A > G), 2 Kb upstream in the promoter region of this gene, causes decrease in the concentration of the product coded by this gene; hence, it may be responsible for impairments in normal function of the gene, ultimately leading to disease condition. Keeping in view the importance of APOA5 gene, the aim of the present study was to examine the association of genetic variant rs662799 of APOA5 gene with two quantitative traits simultaneously, viz. body mass index and blood pressure.ResultsThe study involved a population of 246 subjects from North Indian region. Measurements of morphometric and physio-metric parameters were recorded using standard measures. Genotyping of APOA5 gene polymorphism (rs662799) using Tetra-primer amplification refractory mutation system PCR was performed. Statistical analyses were carried out using MS-Excel and SigmaPlot, and significance level was setup as p < 0.05. The allelic distribution of rs662799 polymorphism in this population was 77% for major allele (A) and 23% for minor allele (G). Significant association of rs662799 with increased body mass index and blood pressure was observed, with the presence of allele G. Under recessive genetic model, rs662799 polymorphism conferred a 17.71-fold risk of elevated body mass index (OR = 17.71, p < 0.001, CI (95%) = 4.05–77.46), and for increase in blood pressure, 3.79- and 3.83-fold risk of systolic blood pressure and diastolic blood pressure (OR = 3.792, p = 0.023, CI (95%) = 1.25–11.509 and OR = 3.83, p = 0.012, CI (95%) = 1.375–10.68, respectively) was observed. Under dominant genetic model, it showed a 3.060-fold risk of increase in body mass index (OR = 3.060, p < 0.001, CI (95%) = 1.78–5.25).ConclusionsG allele of rs662799 of APOA5 gene showed significant susceptible association with BMI and BP. This study may be helpful for clinicians and researchers to investigate the diagnostic and prognostic value of the gene in question.

Morphometric Traits of the Sagittae of Big Scaled Silverside (Atherina boyeri (Risso, 1810)) Captured from the East Coast of Libya

Morphometric Traits of the Sagittae of Big Scaled Silverside (Atherina boyeri (Risso, 1810)) Captured from the East Coast of Libya

Effects of temperature and subspecies during critical early life history stages of largemouth bass (Micropterus salmoides)

Largemouth bass (LMB), Micropterus salmoides, is the most popular sportfish in the United States, with an expanding global food market. Farmers traditionally raise LMB in earthen ponds that are often plagued with high mortality during the early life history stages. Replacing initial pond stages with intensive indoor culture would streamline production and minimize losses. Our objectives were to (i) identify an optimal thermal regimen for rearing LMB in an indoor recirculation aquaculture system (RAS), (ii) assess the performance of Florida vs. Northern LMB for RAS culture, and (iii) elucidate thermally induced phenotypic changes and inter-linked expression of targeted genes involved in early development. Using RAS, Florida and Northern LMB were raised at 21 °C, 24 °C, and 27 °C. Fish were randomly sampled at 2 to 28 days post-hatch (dph) for total length (TL), body area (BA), myotome height (MH), jaw length (JL), eye diameter (ED), oil droplet area (ODA), and yolk area (YA). Condition index, yolk utilization efficiency (YUE), and yolk utilization rate (YUR) were calculated. Wet weight and survival were taken at 29 dph with expression of targeted genes [growth hormone (gh), insulin-like growth factor 1 (igf1), heat shock protein 70 (hsp70)] determined at 8 and 28 dph. There was a temperature effect on all morphometric traits, where both subspecies increased in size over the temperature gradient, with the largest morphometric traits (TL, BA, MH, JL, ED) detected at 27 °C. Larvae had smaller remaining ODA and YA reserves as temperatures increased. Larvae reared at 27 °C utilized their yolk at faster rates and were most efficient at converting yolk reserves to body size. Northern LMB had higher YUE than Florida LMB and were typically larger and heavier at 29 dph. LMB reared at 21 °C had higher survival than those at 24 or 27 °C, while Northern LMB had higher survival than Florida LMB. For Florida LMB, no differences in gh and igf1 were detected across temperatures at 8 dph. However, at 28 dph, these genes were upregulated at 27 °C, while expression of hsp70 was downregulated at 24 and 27 °C. Northern LMB showed similar expression patterns, with no significant differences detected. In conclusion, the present study suggests that 1) rearing LMB at 27 °C may improve growth performance during early ontogeny, and 2) Northern LMB may be selected for faster growth when reared in an indoor RAS.

A new species of Phyllurus leaf-tailed gecko (Lacertilia: Carphodactylidae) from Scawfell Island, mid-east Queensland, Australia

A recent targeted reptile survey of Scawfell Island, in the South Cumberland Group, revealed a species of Phyllurus gecko that could not be morphologically assigned to any described species. Here I describe this as a new species, Phyllurus fimbriatus sp. nov., based on differences in a number of morphometric and scalation traits from congeners. Phyllurus fimbriatus sp. nov. is restricted to deeply-piled boulder habitat under rainforest canopy on Scawfell Island, approximately 50 km offshore from Mackay in mid-east Queensland. A survey in rocky, rainforest habitat on nearby Carlisle Island failed to find the species, and other nearby islands appear to lack sufficiently deep rock outcropping to support the species. Phyllurus fimbriatus sp. nov. is known from two small patches of habitat on Scawfell Island, but it is common within these, and is likely to be found in other suitable habitat patches on the island. Based on assessment of imagery, the total area of habitat occupied may be < 1 km2. The island is protected within South Cumberland National Park, but fire encroachment from adjacent dry sclerophyll habitats, climate change, competition from introduced Asian House Geckos (Hemidactylus frenatus Duméril & Bibron, 1836), and poaching are potential threats.

The Effect of Water Stress on the Glucosinolate Content and Profile: A Comparative Study on Roots and Leaves of Brassica oleracea L. Crops

Drought is one of the major challenges of global crop production, and its severity is increasing because of climate change. This abiotic stress is an important target for Brassica species, which are generally grown in arid and semi-arid climates. This study was conducted to investigate the effects of water deficit on a set of accessions belonging to the Brassica core collection of the EU H2020 BRESOV project, represented by Brassica oleracea L. crops and Brassica oleracea complex species (n = 9). In particular, the variation in the amount and profile of the glucosinolates (GLSs) compounds was analyzed on the root and the leaf tissues. The plant morphometric traits and GLSs amount and profile were detected for the plants grown in cold greenhouse in Catania (Sicily) during the autumn–winter season for ten weeks. The results showed a wide qualitative and quantitative variation among the Brassica accessions. The GLSs profile varied qualitatively and quantitively among both genotypes and portions of the plants (hypogenous-root and epigeous-leaf). Plants grown under drought stress, for the last two weeks of the growing cycle under consideration, showed a higher amount of GLS in their leaves (190.1 ± 8.9 µmol. g−1 d.w.) compared to their roots (17.3 ± 1.9 µmol. g−1 d.w.). Under water stress conditions, the highest increase in the glucosinolate amount was detected in broccoli (the accession BR1) with 85.4% and in cauliflower (the accession CV1) with 72.8% in the roots and leaves, respectively. Positive correlations were found between the major leaf and root GLSs identified. The selection of chemotypes allows for an important time reduction during the breeding programs after crossing accessions with the specific profiles of glucosinolates.

Determination of optimum dose based of biological responses of lethal dose (LD25, 50, 75) and growth reduction (GR25, 50, 75) in ‘Yaghouti’ grape due to gamma radiation

Ionizing radiations are a helpful technique and have improved financial potential in developing new and unique commercially important fruit tree varieties. The ‘Yaghouti’ grape cuttings were treated with 0 (control), 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy gamma ray (γ) doses. The objectives of this study were to (1) investigate the effects of γ radiation on the survival rate, leaves, shoots, and root morphometric traits after γ irradiation; (2) Measurement of the 25, 50, and 75% lethal dose and 25, 50, and 75% growth reduction dose based on leaves, shoots, and root morphometric characteristics to estimate grape radiosensitivity; (3) Ultimately, determining the optimum dose of γ irradiation based biological responses (LD25, 50, 75 and GR25, 50, 75) in Yaghouti grape. The findings demonstrate that the lethal dose of the ‘Yaghouti’ was 18 Gy for LD25, 30 Gy for LD50, and 48 Gy for LD75, respectively. Furthermore, our findings showed that increasing the γ dose had a harmed ngative effect on vine growth, as evidenced by a decrease in plant height, root number, root volume, leaf area, aerial biomass, root biomass, and internode number of the ‘Yaghouti’ grape plants. Our results showed that between the aerial parts of vines, the leaf area and aerial biomass had higher radiosensitivity than plant height and other aerial parts of the plants based on data from GR25, GR50, and GR75. Moreover, GR studies of root characteristics revealed that root number and biomass root had higher radiation sensitivity than root volume. According to biological responses (LD25, 50, 75 and GR25, 50, 75) in the ‘Yaghouti’ grape, 30 Gy of γ radiation is the optimum dose for preliminary mutagenesis investigations.

PRINCIPAL COMPONENT ANALYSIS OF KAIL SHEEP BASED ON BODY MEASUREMENTS

The information on principal component analysis (PCA) of kail sheep was generated using 16 different morphomteriacal traits. A total of 368 Kail sheep individuals were selected from different ecological zone of Azad Jammu Kahmir during the summer of 2020. The coefficient of correlation between these traits were found highly correlate with other morphometric traits of this breed. The PC extract two components and explaning total variance of 67 % and 60% respectively. PC1 has high variance while PC2 high association eith these traits. The application of PC analysis in kail sheep reduce the number of factor and variable a draw a more informative information about morphometric traits. These component can be used in breeding and conservation programme of this breed.

Weight Prediction of Landlly Pigs from Morphometric Traits in Different Age Classes Using ANN and Non-Linear Regression Models

The present study was undertaken to identify the best estimator(s) of body weight based on various linear morphometric measures in Landlly pigs using artificial neural network (ANN) and non-linear regression models at three life stages (4th, 6th and 8th week). Twenty-four different linear morphometric measurements were taken on 279 piglets individually at all the stages and their correlations with body weight were elucidated. The traits with high correlation (≥0.8) with body weight were selected at different stages. The selected traits were categorized into 31 different combinations (single, two, three, four and five) and subjected to ANN modelling for determining the best combination of body weight predictors at each stage. The model with highest R2 and lowest MSE was selected as best fit for a particular trait. Results revealed that the combination of heart girth (HG), body length (BL) and paunch girth (PG) was most efficient for predicting body weight of piglets at the 4th week (R2 = 0.8697, MSE = 0.4419). The combination of neck circumference (NCR), height at back (HB), BL and HG effectively predicted body weight at 6 (R2 = 0.8528, MSE = 0.8719) and 8 (R2 = 0.9139, MSE = 1.2713) weeks. The two-trait combination of BL and HG exhibited notably high correlation with body weight at all stages and hence was used to develop a separate ANN model which resulted into better body weight prediction ability (R2 = 0.9131, MSE = 1.004) as compared to age-dependent models. The results of ANN models were comparable to non-linear regression models at all the stages.

Morphometric characters of Platanus × acerifolia (Aiton) Willd. leaves under the heterogeneous urban environments in the Republic of Srpska

Urban environments are often polluted with numerous contaminants originating from extensive traffic, industry and heating systems. London planetree (Platanus × acerifolia (Aiton) Willd.) is considered as a tree species resistant to air pollutants and climate changes in urban environments. Its resistance is based on the traits of adaptation to dry and warm conditions, bioindication of the degree of urban pollution and plant species with an important role in the nature-inspired solutions for urban areas sustainability. Therefore, this study aims to determine the differences in the morphometric traits of the leaf blade of London planetree, regarding the urban conditions of several cities in the Republic of Srpska. The study was carried out during May-June 2019. Trees for leaf sampling were selected in urban parks and alleys surrounded by high-traffic density streets. At the morphological level, the following leaf blade traits were analyzed: leaf blade length (L), maximum width of the axis of the midrib (W), distance from the midrib to the right margin (X), distance from the midrib to the left margin (Y), distance from the right lateral vein to the main vein (WR), distance from the left lateral vein to the main vein (WL), length of the right lateral vein (RL) and length of the left lateral vein (LL). The results showed that foliar comparisons have been useful in the determination of morphological differences of urban trees influenced by contrasting urban environments. Our results indicate that, based on leaf blade morphometric characters, Bijeljina was distinguished as a separate urban area, Doboj and Prijedor were grouped, while Banja Luka and Trebinje formed the second group. The results indicate the importance of applied analysis in the biological monitoring of the pollution level of urban environments.

Mothers of small-bodied children and fathers of vigorous sons live longer.

Life-history traits (traits directly related to survival and reproduction) co-evolve and materialize through physiology and behavior. Accordingly, lifespan can be hypothesized as a potentially informative marker of life-history speed that subsumes the impact of diverse morphometric and behavioral traits. We examined associations between parental longevity and various anthropometric traits in a sample of 4,000-11,000 Estonian children in the middle of the 20th century. The offspring phenotype was used as a proxy measure of parental genotype, so that covariation between offspring traits and parental longevity (defined as belonging to the 90th percentile of lifespan) could be used to characterize the aggregation between longevity and anthropometric traits. We predicted that larger linear dimensions of offspring associate with increased parental longevity and that testosterone-dependent traits associate with reduced paternal longevity. Twelve of 16 offspring traits were associated with mothers' longevity, while three traits (rate of sexual maturation of daughters and grip strength and lung capacity of sons) robustly predicted fathers' longevity. Contrary to predictions, mothers of children with small bodily dimensions lived longer, and paternal longevity was not linearly associated with their children's body size (or testosterone-related traits). Our study thus failed to find evidence that high somatic investment into brain and body growth clusters with a long lifespan across generations, and/or that such associations can be detected on the basis of inter-generational phenotypic correlations.

Uganda chicken genetic resources: I. phenotypic and production characteristics.

The extent of diversity in the indigenous chicken breeds of Uganda was assessed for their potential utilisation in breeding programmes. A total of 293 indigenous-chicken-keeping households in villages across 35 districts forming 12 sub-regions of the four regions were randomly sampled for 586 mature chickens of both sexes. While only 20.8% of households were headed by women, 40.6% of indigenous chicken keepers were women. The production objectives mainly focused on chickens as sources of income from sales and household food. The chickens were predominantly managed in a scavenging (94.2%) feeding system in a mainly (96.9%) mixed crop-livestock system. The average flock size was 19.80 ± 1.21 chickens with 3.83 ± 0.29 laying hens, each producing an average of 13.41 ± 0.20 eggs/clutch and 40.3 ± 0.6 eggs/annum of 5.20 ± 0.03-month egg production age. Normal-feather strains predominated (>90%), with scattered incidences of naked neck, frizzles, polydactyl, and ptilopody traits in both sexes. Dark (49.0% hen; 43.8% cock) and white (38.3% hen; 42.4% cock) skin colours were most common among the chickens compared to yellow skin. However, yellow-coloured shanks were proportionally the most observed (41% cock; 29% hen). Orange and brown iris (eye) colours were the most common in both sexes. The hens commonly had small round earlobes with varying colours, while cocks had large oval-shaped, mainly red (70%) earlobes. The single-comb type was dominant in both sexes, with wattles almost universally present. Frizzle and polydactyl allele frequencies were significantly lower (p < 0.05) than the expected Mendelian proportions, indicating a possible state of endangerment. Meanwhile, the estimated allele frequencies of ptilopody, tufted-crest, and rose comb alleles in the population were similar (p > 0.05) to the expected Mendelian frequencies. However, these strains did not show any significant (p > 0.05) influence on the body weight or the linear morphometric estimates except for being marginally higher than the normal strains. The phenotypic correlations of body weight and morphometric traits ranged from 0.457 to 0.668 and 0.292 to 0.454 in cocks and hens, respectively. These findings provide hints about the prospects for improved performance with modifications in the production environment. The wide phenotypic diversity would support management efforts for their sustainable utilisation and preservation.

Impact of anthropogenic noise on the survival and development of meagre (Argyrosomus regius) early life stages

The growth of human populations has been driving an unprecedent and widespread increase in marine traffic, posing a real threat to marine biodiversity. Even though we are now aware of the negative effects of shipping noise exposure on fish, information about the impact on their early life stages continues to lack. Meagre (Argyrosomus regius) is a vocal fish that uses estuaries with high levels of anthropogenic noise pollution as both breeding areas and nurseries. Here, the effects of boat noise exposure on the development and survival of meagre larvae were studied.Embryos and larvae were exposed to either noise (boat noise playback) or control treatments (coils producing a similar electric field to the speakers) and hatching rate, survival rate, morphometric traits and stress-related biomarkers, at hatching and at 2 days-post-hatching (dph) were analyzed. Results showed no conclusive effects of the impact of boat noise playback, even though there was an increased lipid droplet consumption and a decrease in body depth at 2dph larvae under this stressor. The assessment of oxidative stress and energy metabolism-related biomarkers at hatching showed a marginal decrease in superoxide dismutase (SOD) activity and no changes in DNA damage or electron transport system activity (ETS), although it cannot be disregarded that those effects could only be visible at later stages of larval development. Whether these morphological and developmental results have implications in later stages remains to be investigated. Further studies with longer exposure and wild meagre could help deepen this knowledge and provide a better understanding of how anthropogenic noise can impact meagre early stages.

Species richness under a vertebral stripe: integrative taxonomy uncovers three additional species of Pholidobolus lizards (Sauria, Squamata, Gymnophthalmidae) from the north-western Colombian Andes.

The systematic study of biodiversity underlies appropriate inference in most other fields of biological research, yet it remains hampered by disagreements on both theoretical and empirical issues such as the species concept and the operational diagnosis of a species. Both become particularly challenging in those lineages where morphological traits are evolutionarily constrained by their adaptive value. For instance, cryptic organisms often conserve or converge in their external appearance, which hinders the recognition of species boundaries. An integrative approach has been adopted to study microgeographic variation in the leaf-litter lizard Pholidobolusvertebralis and test three predictions derived from the evolutionary species concept. Molecular data provided unambiguous evidence of divergence among the three recovered new clades and a common evolutionary history for each of them. The broadly sympatric clades were indeed diagnosable from externally visible traits, such as head scales, adult size, and sexually dimorphic ventral colouration. Also, they barely overlapped on the phenotypic space that summarised 39 morphometric and meristic traits. These clades are described as three species and an available name is suggested for a recovered fourth clade. The geographic distribution of the new and proximate species suggests a role for elevation on evolutionary divergence; it also raises interesting questions on the speciation pattern of an otherwise underestimated cryptic lineage.

Morphostructural Differences between the Historical Genetic Lines of the Spanish Merino Sheep.

The Merino breed, which originates from Spain, is the most emblematic livestock breed in the world, since it is the first with a worldwide extension and has had an important impact on the genetic origin of several of the main current sheep populations. For this reason, it is of vital importance to typify the historical genetic lines of the original Spanish Merino breed and thereby ensure the conservation of its variability. In the present study, we used 337 purebred animals (males and females) registered in the Genealogical Book of the Native Merino Breed. All the animals were descendants of herds from six ancestral genetic lines (Maesso, Egea, Granda, López-Montenegro, Hidalgo, and Donoso). Significant differences were found in all the morphometric traits and indexes between the different genetic lines. Using discriminant analysis, 84% of the animals were classified correctly into their historical genetic lines. Furthermore, the distances between the lines, calculated by a cluster test, showed that Hidalgo, Maesso, and Donoso had the most clearly defined lines, while the Granda, López-Montenegro, and Egea lines were more similar to each other. All this demonstrates the rich genetic variability existing in the genuine gene pool of the Merino sheep breed.