Morphological Examination Research Articles

Effect of helium ion and gamma irradiation on the TL and OSL properties of Tb-doped LiF nanophosphors

Lithium Fluoride (LiF) stands out as a extensively researched material in Thermally Stimulated Luminescence Dosimetry (TLD). This study specifically delves into nanocrystalline Tb-doped LiF TL dosimeters, synthesized through the co-precipitation method, and evaluates their efficacy under He ion and gamma rays radiation. Morphological examination via transmission electron microscopy has revealed cubic-shaped particles. These particles were subjected to irradiation by 120 keV He ion beams ranging from 1014 to 1016 ions/cm2 and gamma rays ranging from 10 to 1000 Gy, facilitating a comprehensive comparative analysis. Deconvolution of the glow curves enabled the extraction of trapping parameters. Under gamma irradiation, the nanophosphor exhibited a distinctive, intense single TL glow peak centered at 400 K with a minor hump at 512 K, diminishing with increasing doses. Conversely, under He ion irradiation, the nanophosphor displayed a peak at 492 K, accompanied by a slight shoulder around 600 K. Additionally, gamma doses displayed a highly promising response to Optically Stimulated Luminescence (OSL). Fading studies further underscore its suitability for radiation dosimetry applications. The unique characteristics of the TL glow curve, dose-response behaviours, and OSL responses highlight the versatility and effectiveness of these nanophosphors in radiation dosimetry, making significant contributions to advancements in this field.

Comparison of methods for morphological evaluation of sheep spermatozoa using the SPERM BLUE kit and Romanovsky-Giemse staining

The purpose of this study is to test and comparatively evaluate the method of morphological examination of ram sperm using a differentiated staining Sperm Blue and Romanovsky-Giemsa staining. The sperm of Dorper rams was collected and examined. The obtained samples (n=10) were diluted in a ratio of 1:100 (PBS solution) to conduct a morphological assessment of sperm. Two methods were used for the morphological assessment of spermatozoa: a set of differentiated staining Sperm Blue (Microptic, exposure of the smear in the fixative – 10 minutes, in the main dye - 18 minutes) and Romanovsky-Giemsa staining (exposure of the smear in the fixative - 10 minutes, in the main dye – 20 minutes). After routine staining, the smears were washed with distilled water, dried, and microscopy was performed (magnification using a 100x10 immersion objective, oil immersion), followed by a manual method of counting the number of morphological forms of sperm. According to the results of a comparative analysis of stained smears, staining with Sperm Blue allows for a detailed morphological study and differentiation of teratozoospermic characteristics. When stained with Romanovsky-Giemsa, normal spermatozoa were clearly visible, but the acrosome was poorly visualized. Manual calculation of the percentage of normal sperm using both dyes resulted in the same decimal result. A statistically insignificant difference in head and tail defects was found between the dyes used (p = 0.41 and p = 0.77, respectively): Romanovsky-Giemsa staining (7.8% and 34.1%) detected these defects more often than Sperm Blue (6.3% and 32.9%). Considering the results obtained, the use of the Sperm Blue dyes dye in veterinary andrology is relevant, in particular for assesment the morphology of sperm of different animal species with the further development of appropriate protocols in terms of the duration of exposure of the smears themselves to the main dye.

Synthesis of a 3D Porous Multicomponent Bioceramic Scaffold

In this study, 3D porous multicomponent bioceramic scaffolds were fabricated. Hydroxyapatite (HA) and carbonate-substituted hydroxyapatite (CHA) synthesized from Asian moon scallop shell (Amusium pleuronectes) served as the bioceramics, whereas alginate and chitosan served as the polymeric components. The study was focused on determining whether the presence of polyvinyl alcohol (PVA) in the scaffold exerted an impact. When PVA was not included, the morphological examination revealed that the samples exhibited porous structures characterized by effective pore interconnectivity and a substantial pore size. X-ray diffraction and scanning electron microscopic analyses indicated that the inclusion of PVA led to a decrease in the crystallinity, pore size, and porosity of the scaffolds. Those containing PVA exhibited porosity levels in the range of 56%–60%, and pore sizes ranged from 42 to 90 µm. These properties may provide advantages for the scaffold with respect to the ability for cell migration and cell attachment, thus enhancing new bone formation. Moreover, the compositions of HA and chemically modified HA (CHA) within the scaffold influenced the crystallinity and uniformity of the scaffold morphology. This finding suggests the potential for crafting a customized porous bioceramic scaffold based on specific compositions.

Patagonian partnerships: the extinct Dusicyon avus and its interaction with prehistoric human communities.

The southern Mendoza province, located in the northern region of Patagonia, was inhabited by hunter-gatherer groups until historic times. Previous archaeological studies have reported canid remains among faunal assemblages, which were assumed to be part of the human diet. However, the taxonomic identification and significance of these canids within human groups have raised questions. In this study, we used ancient DNA analysis, morphological examination and stable isotope analysis (δ13Ccol and δ15N) to re-evaluate the taxonomic assignment of a canid discovered at the Late Holocene burial site of Cañada Seca. Previous morphological identifications suggested that it belonged to the genus Lycalopex, but our results conclusively demonstrate that the individual belongs to the extinct fox species Dusicyon avus. This finding expands Dusicyon avus' known geographical distribution to Patagonia's northern extremity. Furthermore, statistical predictions based on genetic divergence undermine the hypothesis that hybridization between Canis and Dusicyon, facilitated by the introduction of domestic dogs, played a role in the extinction of Dusicyon species. On the other hand, our findings indicate that a Dusicyon avus individual shared a similar diet and was probably buried alongside humans, suggesting a close relationship between the two species during their lives and deaths.

Pathogenic Aspergillus Strains Identification and Antifungal Susceptibility Analysis of 452 Cases with Otomycosis in Jingzhou, China.

To study the distribution of pathogenic Aspergillus strains of otomycosis in central China and the identification of their antifungal sensitivity. We collected external ear canal secretions clinically diagnosed as otomycosis from April 2020 to January 2023 from the Department of Otolaryngology-Head and Neck Surgery in central China. The pathogenic Aspergillus strains were identified through morphological examination and sequencing. The antifungal sensitivity was performed using the broth microdilution method described in the Clinical Laboratory Standard Institute document M38-A3. In the 452 clinical strains isolated from the external ear canal, 284 were identified as Aspergillus terreus (62.83%), 92 as Aspergillus flavus (20.35%), 55 as Aspergillus niger (12.17%). In antifungal susceptibility tests the MIC of Aspergillus strains to bifonazole and clotrimazole was high,all the MIC90 is > 16 ug/mL. However, most Aspergillus isolates show moderate greatly against terbinafine, itraconazole and voriconazole. A. terreus is the most common pathogenic Aspergillus strain in otomycosis in central China. The selected topical antifungal drugs were bifonazole and clotrimazole; the drug resistance rate was approximately 30%. If the infection is persistent and requires systemic treatment, terbinafine and itraconazole can be used. The resistance of Aspergillus in otomycosis to voriconazole should be screened to avoid the systemic spread of infection in immunocompromised people and poor compliance with treatment. However, the pan-azole-resistant strain of Aspergillus should be monitored, particularly in high-risk patients with otomycosis.

Psychophysical, electrofunctional, and morphological evaluation in naïve neovascular AMD patients treated with intravitreal anti-VEGF.

The aim of this study was to investigate the retinal morpho-functional characteristics of patients with neovascular wet age-related macular degeneration (nAMD) treated with intravitreal injection (IV) of aflibercept (AFL). The study was conducted on 35 patients previously diagnosed with type 1 nAMD who received a fixed-dosing regimen of aflibercept injections over 12 months. The goal was to assess trends in visual abilities over time by measuring visual acuity (VA), contrast sensitivity (CS), visual evoked potentials (VEPs), and spectral domain-optical coherence tomography (SD-OCT). The same psychophysical, electro-functional, and morphological tests administered at baseline (T0) were repeated 4 to 8 weeks after the last aflibercept injection (Tn), resulting in a total of six examinations. At Tn, all subjects exhibited improved VA for both far and near distances compared to values detected at T0. Similarly, VEP amplitude and latency values at Tn showed a greater P100 improvement than those observed at T0. Additionally, the CS examination at Tn demonstrated improvement, particularly at high spatial stimulation frequencies. The Tn SD-OCT results highlighted a reduction in macular thickness compared to T0 values. This exploratory research indicates that intravitreal injections of AFL, following a fixed-dosing regimen, represent a valuable therapeutic approach for enhancing visual performance. This conclusion is supported by comprehensive statistical analysis of psychophysical, electro-functional, and morphological examinations within the same group of patients with nAMD, as demonstrated for the first time.

Nano therapy for photodynamic cancer treatment of lung, melanoma and cervical cancer stem cells

SignificanceLung cancer is the most dangerous, malignant melanoma is the most aggressive, and cervical cancer has become more common in youngsters. PDT causes light-dependent and ROS-related cell death with non-toxic photosensitizers (PS). PDT can treat many malignancies, but cancer stem cells (CSCs), a subset of tumor cells that can initiate and relapse, may resist treatment. ApproachThis study examined the effects of AlPcS4Cl - nanoPDT using gold nanoparticles (AuNPs) on lung, cervical, and melanoma CSCs. Intracellular localization of the drug compound and biochemical assays including viability, proliferation, cytotoxicity, and Annexin VPI cell death were performed together with morphological examination. ResultsThe results demonstrate increased absorption and localization of the therapeutic molecule in CSCs. A decrease in CSC survival and proliferation, enhanced cytotoxicity, and apoptotic cell death confirmed the drug's efficacy. ConclusionsThe results demonstrated that when AuNPs were utilized as a drug carrier for AlPcS4Cl, the PDT's effects were amplified, culminating in the complete annihilation of CSCs.

A comparative study of the effect of chromium on the oxidation resistance of nano-structured cobalt-aluminium coatings deposited by magnetron sputtering

The experiment studied the effect of chromium on the oxidation resistance of nanostructured cobalt-aluminum coatings, deposited on superalloy Superni-718 by magnetron sputtering. For this, the cobalt-aluminium coated superalloy was subjected to similar conditions of cyclic oxidation at 1173 K as the cobalt-alumimium coated samples with chromium. Further, the influence of substrate temperature on the properties of coatings was examined by comparing the coatings deposited at substrate temperatures of 773 and 973 K during magnetron sputtering. Post-cyclic oxidation tests, the examination of the surface morphology and phase formations was carried using X-Ray Diffraction and Field-Emission Scanning Electron Microscope/Energy Dispersive Spectroscopy. The coating containing chromium displayed the maximum resistance to cyclic oxidation in comparison to the coatings without chromium, as is apparent from the variation in mass per unit area of the superalloy. Further it was observed that increasing the substrate temperature in magnetron sputtering significantly augments the high temperature resistance of the coatings.

Evaluation of Copper Nanoparticle Synthesis by Fusarium oxysporum Antibacterial Activity Against Staphylococcus aureus

The creation of effective, environmentally friendly antimicrobial alternatives is becoming more and more important in light of the development of antibiotic resistance. The objective of the present study was to illustrate the biosynthetic process of copper (II) carbonate nanoparticles, CuCo3 NPs, by using Fusarium oxysporum culture filtrate as an antibacterial activity against multidrug-resistant (MDR) clinical bacterial isolates, Staphylococcus aureus. Fusarium oxysporum isolate was diagnosed by PCR. One hundred and sixty specimens of pathogenic bacteria were collected from different sources (wounds, urine, sputum) then the bacterial isolates were diagnosed as Staphylococcus aureus by conventional morphological examination, biochemical tests, and the Vitek-2 system. Fusarium oxysporum culture filtrate was prepared by a culture of the fungus on Czapek Dox broth media modified by adding cornmeal and incubated for 14 days with shaking at 27±2°C and filtered in the last stages using a Millipore. The biosynthesis of CuCo3 NPs was done by adding 1g of CuCo3 Cu (OH)2 to 10 ml of Fusarium oxysporum culture filtrate. The NPs were diagnosed using modern methods, including UV-VIS, FT-IR, AFM, FE-SEM, and EDX techniques. Multidrug-resistant Staphylococcus aureus was tested on the produced Cu NPs. The results indicated that the prepared CuCo3 NPs inhibited pathogenic bacterial isolates of Staphylococcus aureus at concentration of 500, 250, 125, and 62.5 mg/ml.

Experimental analysis of sugarcane and coconut shell fibre impregnated with glassfiber composite for aircraft application

Natural fibers' adaptability, sustainability, and environmental friendliness make them useful in a variety of sectors and applications. Sugarcane bagasse and coconut shell, which are made from industrial waste, have great promise as a sustainable substitute for traditional materials. How sugarcane bagasse, coconut shell, and glass fiber interact with an epoxy composite matrix is the subject of the present experimental investigation. The Semi Compression Moulding Machine (SCMM) was used to make the following compositions: 50 % matrix, 20 %-30 % sugarcane fiber, 10 %-25 % glass fiber, and 5 %-10 % coconut shell fiber. The ASTM-compliant composite plate structure that was built has a tensile strength ranging from 20 to 25 MPa and a tensile fatigue failure stress of 7 to 8 MPa, all tested over a range of 14,000 to 16,000 cycles. The scanning electron microscope (SEM) was used for morphology examination, while X-ray diffraction (XRD) was employed for crystalline properties. The use of natural fibers impregnated into glass fiber reinforced composites allowed for the creation of low density, very stable materials, which were essential for the skin and control surfaces of aircraft.

Gastric Type Endocervical Adenocarcinoma With Concurrent High-Grade Squamous Intraepithelial Lesion: A Clinicopathologic Study of Three Patients.

We investigate gastric-type endocervical adenocarcinoma (ECA), a prominent HPV-independent adenocarcinoma, and its coexistence with high-grade squamous intraepithelial lesion (HSIL) through the examination of three such tumors. In this study, we conducted an in-depth review of three patients with gastric-type ECA, each associated with high-risk HPV infection as detected on Pap smears. We detailed the clinical and pathological features of each patient and utilized RNAscope for high-risk HPV testing to ascertain HPV status in both gastric-type ECA and HSIL components. Immunohistochemistry with p16, p53, and other biomarkers was also applied. The gastric-type ECA component, characterized by well-differentiated glands with abundant, clear to eosinophilic cytoplasm, distinct cellular borders, and pale nuclei with conspicuous nucleoli, tested negative for both p16 and high-risk HPV, unlike the concurrent HSIL components which were positive. Additionally, two tumors showed aberrant p53 protein expression in the gastric-type ECA areas, and elevated carbohydrate antigen19-9 levels were noted in two patients. Treatment consisted of total abdominal hysterectomy and bilateral salpingo-oophorectomy, supplemented by chemotherapy and/or radiation, with disease-free intervals of 24, 12, and 40 months post-treatment, respectively. This study highlights the critical need for meticulous diagnostic protocols that combine morphological examination, immunohistochemistry, and HPV RNA in situ hybridization. The rarity of gastric-type ECA coexisting with HPV infection underscores the necessity for continuous research and vigilant monitoring in the field of gynecological oncology.

Studies on Anticancer Effect of Methanolic Leaf Extract of Justicia gendarussa on Lung Cancer Cell Line

ABSTRACT Objective: The aim of study’s goal was to look into the anticancer efficacy of a methanolic extract of Justicia gendarussa against a lung cancer cell line. Materials and Methods: Cell viability assays and cell and nuclear morphology examinations were used to evaluate the anticancer efficacy against methanolic extract of Justicia gendarussa on lung cancer cell lines. The IC50 doses were calculated using different concentrations of Justicia gendarussa extract (0, 10, 20, 40, 60, and 80 μg/mL). Results: The results of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay revealed that the percentage of viability in treated cells was significantly lower as compared with untreated control groups, which represented as 100%, and an inhibitory concentration of 40 μg/mL was observed. Under a phase-contrast microscope, morphological changes revealed cell shrinkage and cytoplasmic membrane blebbing. The apoptotic nuclei (intensely colored, broken nuclei, and compacted chromatin) were examined under a fluorescence microscope. Conclusions: The outcome of the research work on Justicia gendarussa was investigated for anticancer properties. The results revealed the proapoptotic and cytotoxic effects of Justicia gendarussa extract on lung cancer cell lines. From the above results and findings, it could be concluded that the Justicia gendarussa methanolic leaf extract exhibited potent anticancer activity against a lung cancer cell line. Further study needs to be conducted to investigate the active chemicals in the extract as well as the molecular mechanisms underlying its anticancer benefits.

A study of morphological, optical, and photoluminescence properties of ZnS thin films doped Mn and ca

In this study, zinc sulfide (ZnS) thin films were deposited via physical vapor deposition (PVD) under high vacuum conditions, incorporating dual dopants manganese (Mn2+) and calcium (Ca2+). Morphological examination by AFM revealed that Mn2+ promoted a uniform and smooth film structure, whereas Ca2+ induced surface roughness. Optical characterization using ultraviolet–visible spectroscopy showed high transparency in the visible range, with doping leading to significant alterations in the bandgap energy. Photoluminescence investigations displayed prominent emission peaks at 385 nm, 448 nm, and 560 nm, characteristic of ZnS bandgap emissions, as well as an additional peak at 600 nm due to the novel dopant's influence. X-ray fluorescence (XRF) and Fourier transform infrared (FTIR) analyses verified the presence of ZnS and other constituents. Doping improved the optical and luminescent characteristics, offering potential for use in optoelectronics, light-emitting diodes (LEDs), and advanced technology development. This customizable approach to ZnS thin films presents innovative opportunities within material science and device manufacturing.

Analysis of Stones in patients presenting with kidney stone disease: A study from Khairpur

The study was comprised of the chemical analysis of certain constituents in the 100 renal calculi samples that were collected from the District Khairpur randomly and the study of the samples was evaluated by the frequency of occurrence on the basis of age and Sex and their morphological composition. A quantitative analysis was derived by the mean of highly sensitive techniques and the ethics was also included. One-hundred calculi samples were obtained from patients admitted in Khairpur Medical college (KMC) and Gambat Institute of Medical Sciences (GIMS) from District Khairpur, upon which 68 were male and 32 were female Urolithaisis patients. The most cases were observed in the age group between 16-30. The calculi samples were subjected for qualitative as well as quantitative analysis, for determination of calcium, phosphorus and oxalate that are the main constituents of the calculi. Morphological examination was also conducted on the basis of color, size, shape and weight.

The effect of preanalytical phase on the stability of the osmotic fragility and morphological changes in erythrocytes in cattle (Bos taurus)

Hereditary spherocytosis in cattle is a disorder that results from a nonsense mutation in SLC4A1 gene that encodes band 3 membrane protein. This membranopathy leads to a production of spherically-shaped RBCs with reduced deformability and shorter lifespan in circulation. The laboratory diagnosis of hereditary spherocytosis commonly rely on erytrocyte osmotic fragility test (OFT), which is time and work consuming and can be influenced by the preanalytical factors. The aim of the study was to determine the effect of the preanalytical phase on the stability of the osmotic fragility and morphological changes of bovine erythrocytes. The blood was collected from the tail vein from Holstein-Friesian cows (n = 20) into tubes with EDTA or lithium heparin. The erythrocyte resistance to hemolysis was measured by osmotic fragility test immediately after blood collection and 24, 48 and 72 hours after storage at 4oC or room temperature. The thin blood smears were also prepared, stained and examined for cell morphology.The results showed that bovine erythrocyte osmotic fragility remains stable during 24 hours storage at 4oC regardless of the anticoagulant used. It suggests the possibility of performing OFT the day after blood collection when storing blood at 4oC. It was also found that bovine erythrocytes stored at room temperature have reduced osmotic resistance. However, heparin better preserves osmotic resistance of bovine erythrocytes at room temperature. Erythrocytes that are stored undergo changes during the storage period. The microscopic examination of cell morphology revealed the transformation of bovine discocytes into different stages of echinocytes, which rapidly occurs during the first three days of storage.

Spectrum of Chronic Gastritis Based on Morphological Examination of Gastric Biopsies

Aim: in the retrospective study to assess the frequency of various gastritis variants based on the results of morphological examination of gastric biopsies over a 5-year observation period.Methods. The study included 3162 individuals who underwent esophagogastroduodenoscopy with biopsy collection between 2017 and 2022. Pathological examination of biopsies was conducted using the updated Sydney system, determining the histological variant and, when possible, the etiology of gastritis. In some cases, chronic gastritis was assessed using the OLGA/OLGIM system.Results. The most frequently diagnosed type was active H. pylori-associated gastritis (36.7 %), followed by chronic atrophic gastritis with intestinal metaplasia in cases where H. pylori infection was not detected (28.4 %), including immunohistochemical examination, classified as gastritis after successful H. pylori eradication. Atrophic forms of chronic gastritis constituted 34.8 %. Minimal or mild changes close to normal histological characteristics were оbserved in 19.2 % of patients. Reactive gastropathy was present in 7.6 % of cases. Autoimmune gastritis ranked fourth in frequency, with a relatively high percentage of observations (8.6 %).Conclusion. According to the analysis of gastric biopsies in the Russian population, a high frequency of H. pylori-associated gastritis was observed, and autoimmune gastritis is not uncommon. The high prevalence of atrophic gastritis emphasizes the importance of dynamic patient monitoring within cancer prevention programs.

Morphological Evaluation and Immunohistochemical Analysis of the Reparative Potential of the Buccal Fat Pad.

Background and Objectives: There are many surgical techniques for oroantral communication treatment, one of which is the buccal fat pad. Of particular interest is the high reparative potential of the buccal fat pad, which may be contributed to by the presence of mesenchymal stem cells. The purpose of this work is to evaluate the reparative potential of BFP cells using morphological and immunohistochemical examination. Materials and Methods: 30 BFP samples were provided by the Clinic of Maxillofacial and Plastic Surgery of the Russian University of Medicine (Moscow, Russia) from 28 patients. Morphological examination of 30 BFP samples was performed at the Institute of Clinical Morphology and Digital Pathology of Sechenov University. Hematoxylin-eosin, Masson trichrome staining and immunohistochemical examination were performed to detect MSCs using primary antibodies CD133, CD44 and CD10. Results: During staining with hematoxylin-eosin and Masson's trichrome, we detected adipocytes of white adipose tissue united into lobules separated by connective tissue layers, a large number of vessels of different calibers, as well as the general capsule of BFP. The thin connective tissue layers contained neurovascular bundles. Statistical processing of the results of the IHC examination of the samples using the Mann-Whitney criterion revealed that the total number of samples in which the expression of CD44, CD10 and CD133 antigens was confirmed was statistically significantly higher than the number of samples where the expression was not detected (p < 0.05). Conclusions: During the morphological study of the BFP samples, we revealed statistically significant signs of MSCs presence (p < 0.05), including in the brown fat tissue, which proves the high reparative potential of this type of tissue and can make the BFP a choice option among other autogenous donor materials when eliminating OAC and other surgical interventions in the maxillofacial region.

Morphological peculiarities of regeneration phases and dynamics of growth factor levels at using 2-ethyl-6-methyl-3-hydroxypyridinium n-acetyl-6-aminohexanoate for rat skin burns repairation

BACKGROUND: It was found that new derivatives of acexamic acid — N-acetyl-6-aminohexanoate of silver and 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate stimulate regeneration and mineralization of bone tissues in osteoporosis. However, to date, the effect of 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate on the features of reparative histogenesis and the relationship of its stages with the levels of growth factors in regenerating tissues during skin restoration after thermal burn has not been studied.&#x0D; AIM: To estimate the morphology dynamics of skin burn healing and its relationship with the levels of vascular endothelial growth factor and basic fibroblast growth factor growth factors in rat regenerating tissues to characterize the reparative properties of 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate.&#x0D; MATERIALS AND METHODS: 63 nonlinear rats were used to establish thermal burn wound model according to standard methodics. Burn wounds of the experimental group animals were treated with 2% ointment of 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate daily, control group 1 — the burns were not treated, control group 2 — ointment base was applied daily. Morphological examinations of regenerating tissues from the burn area were carried out on days 7, 14 and 21 of the experiment after standard sample preparation. The concentrations of growth factors in homogenates of regenerating tissues were determined by Enzyme Immunoassay.&#x0D; RESULTS: The inflammation phase is characterized by vascular endothelial growth factor active production and release from cells and morphologically accompanied by early neovasculogenesis in granulation tissue and significant differences in its thickness in rats of the experimental group. During the proliferation phase, activation of fibroblast reproduction and differentiation was associated with an increase in basic fibroblast growth factor levels and the maintenance of elevated vascular endothelial growth factor levels in all groups, especially high in experimental group rats. The epithelialization phase was accompanied by a statistically significant decrease in the content of basic fibroblast growth factor and vascular endothelial growth factor in regenerating skin tissues by 1.9 and 1.8 times, respectively, in comparison with the control groups.&#x0D; CONCLUSIONS: The obtained experimental data indicate 2% ointment with 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate has a reparative potential, as evidenced by the accelerated reduction both the area of burn defects and the average time for their healing in the experimental group compared to the control groups.

Preparation of the endometrium in the programs of auxiliary reproductive technologies for sterility in women

Background. The most important stage in the treatment of infertility is the introduction into clinical practice of the methods of in vitro fertilization (IVF), which made it possible to implement the function of childbirth in almost all forms of female infertility. The introduction of endoscopic technologies made it possible to significantly expand diagnostic possibilities and clarify the nature of intrauterine pathology when using assisted reproductive technologies. Purpose – of the research should be to increase the effectiveness of the treatment of female infertility when using assisted reproductive technologies and hysteroscopy by creating conditions in the endometrium for embryo implantation. Materials and Methods. A study was conducted in 42 patients with infertility aged 32 to 38 years. In all women, the morphological state of the endometrium was determined after diagnostic hysteroscopy. The patients were divided into 2 clinical groups. Group 1 included 28 women who were in a state of preparation for IVF after hysteroscopy with changes characteristic of endometrial pathology, which were found during ultrasound. The second group included 14 patients in whom, according to ultrasound, no structural changes of the endometrium were noted. These patients had a failed IVF attempt. In the next cycle, the women underwent a diagnostic hysteroscopy due to the lack of pregnancy after the first IVF attempt. Results. Group 1 patients (n = 28) with endometrial changes before IVF showed that the causes of pathology were: endometrial hyperplasia (53.6%); chronic endometritis (21.4%), endometriosis (25%). Therefore, before stimulation of superovulation, a hysteroscopy was performed to confirm the morphological diagnosis. In patients of the 2nd group (n = 14) after hysteroscopy and histological examination, pathological changes of the endometrium were found in all patients. Chronic endometritis occurred in 5 cases (35.7%), micropolyposis of the endometrium – in 6 (42.9%), endometriosis – 3 (21.4%). Clinical pregnancy occurred in 17 patients (60.7%) in the first group, in 9 (64.2%) in the second group. Conclusions. Hysteroscopy with morphological examination of the endometrium remains the main method of diagnosing intrauterine pathology. Diagnosis of infertility by hysteroscopy plays a very important role in the normalization of reproductive function during in vitro fertilization (IVF). After an unsuccessful IVF attempt, in the absence of pathological changes in the endometrium, a diagnostic hysteroscopy should be performed to rule out endometrial pathology.

A Case of Lymphoplasmacytic Cell Lymphoma Characterized by Marked Nuclear Fission and Brain Gyrus Nuclei of Lymphocytes

A 67-year-old male was admitted to the hospital due to fatigue and thrombocytopenia. Peripheral blood cells analysis showed the white blood cells count of 2.99 × 109 /L, red blood cell (RBC) count of 2.08 × 1012/L, platelet count of 15 × 109 /L. β2- microglobulin of serum was 5.50 mg/L. Whole-body positron emission tomography-computed tomography suggested altered reactivity in the lymph nodes of the bilateral neck. Morphological examination of bone marrow (BM) indicated the presence of abnormal smaller lymphocytes, accounting for 12.5% of the total nucleated cells. Strikingly, nuclear fission or brain gyrus nuclei were prominently observed in all abnormal lymphocytes (Figure 1, original magnification 1000×; Wright–Giemsa stain). The proportion of plasma cells was 0.5%, and no morphological abnormalities were observed. Additionally, some RBCs were exhibited a rouleaux arrangement. Flow cytometry analysis of BM identified 16.12% of κ-restricted B lymphocytes expressing CD5- , CD10- , CD19+ , CD20+ . A minimal proportion of 0.03% plasma cells with κ/λ ratio of 1.07. Consistent results were confirmed by immunohistochemistry in the BM biopsy. Immunofixation electrophoresis revealed abnormal monoclonal bands in the IgM and κ lanes. Furthermore, mutations of MYD88 (L265P), CXCR4 (T318fs), and TP53 (R306X) were identified through next-generation sequencing analysis. A diagnosis of lymphoplasmic cell lymphoma/waldenström macroglobulinemia (LPL/WM) was established.