Moringa Leaves Extract Research Articles

Effect of Bacterial Biofertilizer, Buffalo’s Organic Manure and Moringa Leaves Extract Spray in Vegetative Growth Characteristics of Local Orange Transplants

Effect of Bacterial Biofertilizer, Buffalo’s Organic Manure and Moringa Leaves Extract Spray in Vegetative Growth Characteristics of Local Orange Transplants

The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone

The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone

Response of "Salakhani" Pomegranate Trees to Spraying with Moringa Leaves Extract, Garlic, and Turmeric

The present study during seasons in 2022 to study the extracts effect of moringa (Moringa oleifera) leaves at (2-, 4-, and 6-ml l-1), garlic (Allium sativum) cloves (5, 10, and 15 ml l-1), and turmeric (Curcuma longa) at (5, 10, and 15 ml l-1) sprayers on Salakhani pomegranate. Thirty uniform trees were selected, and each tree was considered as an experimental unit using a randomized complete block design (RCBD) with three replicates. The results showed that spraying pomegranate trees with moringa leaves extract with 2 and 6 ml l-1 and all doses of turmeric extract significantly increased surface area and dry weight of leaf, except 6-ml L-1 and 15 ml l-1 of morenga leaves extract of turmeric extract increased leaf dry weight non-significantly compared to the control. As well as all garlic clove extract levels increased the surface area and dry weight of the leaf significantly compared to the control. Moreover, all plant extract treatments were effective in increasing the number of fruits and yield per tree compared to the control. Additionally, most plant extract treatments improved all chemical parameters including total soluble solids, titratable acidity, pH, total sugar, and anthocyanin content as compared to the control.

The Effect of Organic and Chemical Fertilizers and Moringa Leaves Extract on the Content of some Compounds and Nutrients in Olive Leaves

The study was Conducted in the Orchard of the Department of Horticulture and Landscape Engineering at the College of Agriculture-University of Anbar for the season 2021-2022 on Olive Trees, cv. K18, at the age of 8 years, to find out the effect of adding Organic fertilizer at levels H0 without addition and H1, adding Humic acid at a Concentration of 10 g L-1 and H2, adding poultry waste at a level of 8 kg Tree-1 and the addition of NPK in three Concentrations N0 without addition and N1 concentration of 50g Tree-1 and N2 Concentration of 100g Tree-1 and spraying with Moringa Leaves extract with two concentrations M0 without spraying and M1 spraying with a concentration of 4% in the Content of Olive tree Leaves of some compounds and nutrients. A factorial experiment was carried out according to the Randomized Complete Block Design RCBD with three Replications. The results showed the significant superiority of the Treatment of adding poultry waste at the level of 8kg Tree-1 in the Leaves Content of Total Phenols 105.59%, Phenol Oleuropein 92.03%, Nitrogen 1.585%, Phosphorus 0.119%, Potassium 0.831% and Magnesium 0.234%. and Calcium amounted to 1.239%, and the Treatment of adding Chemical fertilizer with a concentration of 100g Tree-1 excelled in all studied traits. As for spraying with Moringa Leaves extract, there were significant differences in the Leaves’ Content of compounds and nutrients. As for the binary and triple interactions between the study factors, they were There are significant differences in most of the studied traits.

Evaluation of the effect of chemical fertilization and some natural extracts on the production of freesia (Freesia refracta L.) plants: a sustainable approach

Effects of different types of fertilizers; traditional NPK (1 g plant−1), 2, 4, 6 and 8 gm L−1 of moringa leaves extract (MLE), 10, 20, 40 and 60 mL L−1 of aloe leaves extract (AL) on vegetative growth, flowering Corms production and chemical constituents of Freesia refracta L. were evaluated during two successive seasons (2020–2021 and 2021–2022). Treating plants with MLE caused increases in plant height, number if leaves and number of flowers by 35.2%, 8.2% and 16.3%, respectively, while treating plants with Al caused increases by 33.2%, 7.9% and 15.4% in the same parameters, respectively, averaged between different concentrations and growth seasons. The results revealed that, treating plants with 8 gm L−1 MLE caused a significant increase in the vegetative growth, flowering, and corms production. These improvement fits with the many goals of the 17 United Nation Sustainable Goals (UNSDGs); especially SDG2 (Zero hunger), SDG13 (climate action) and SDG15 (Life on land). Moreover, the chemical composition of plant was improved significantly.

Antidiabetic Effect of Noni Fruit and Moringa Leaves Extract on Blood Glucose Level, Hepatic and Pancreatic Histological Features of Diabetic Mice

One of the chronic diseases with high blood glucose levels is a characteristic of Diabetes Mellitus. Utilization of herbal ingredients needs to be developed to reduce blood sugar levels in diabetes, and one of them is by using moringa leaf extract and noni fruit. This study aimed to analyze the effect of a combination of moringa leaf extract and noni fruit on blood glucose levels and tissue cytology in diabetic mice. The study was a randomized pretest-posttest control group design using 28 male mice divided into seven groups. The data analysis showed the lowest decrease in blood glucose levels in the group of diabetic mice given noni fruit and moringa leaf extract at a dose of 150mg/20g BW/oral/day for two weeks. The results of histological examination of liver and pancreas tissue showed no tissue is damaged.

Biosynthesis and Characterization of CdO: Ag NPs using Moringa Leaves Extract for Use as Anti-microbial Activity

The current research focuses on synthesizing cadmium oxide (CdO), CdO: Ag nanoparticle (NPs) by green method using the Moringa leaves Extract (MLE) and its anti-microbial activity. Using UV-vis spectroscopy to determine the formation of CdO: Ag NPs where the energy gap of the prepared samples was 2.79, 2.67, and 2.46 for CdO, CdO: Ag 3% and CdO: Ag 5%, respectively. The biological substances in responsibility of capping the produced NPs were identifi ed using FTIR. The NPs were further characterized by X-ray diff ractograms (XRD). The average grain size for CdO, CdO: Ag (3 and 5%) were 15.322, 15.5, and 36.84 nm, respectively, scanning electron microscope (SEM), energy dispersive X-ray (EDX) and atomic force microscope (AFM), were obtained the average diameter 18.88, 43.48, 99.15 nm for CdO, CdO: Ag 3% and CdO: Ag 5%, respectively. The anti-bacterial activity of green synthesized CdO: Ag(NPS) was investigated against (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia) at diff erent concentrations, showing clear inhibition zones from 30 to 38 mm, thereby indicating its inhibitory activity.

THE INHIBITION EFFECT OF MORINGA (Moringa oleifera L.) LEAVES AND RED GINGER RHIZOME (Zingiber officinale Rosc.) PT. "P" ETHANOL EXTRACT ON ENHANCEMENT SERUM CREATININE LEVELS OF MALE WISTAR RATS DYSLIPIDEMIA MODEL

The elevation of serum creatinine levels can be used as a biomarker of decreased kidney function. Dyslipidaemia is one of the causes of decreased kidney function. Long-term consuming statins has been shown to lower cholesterol levels but can increase serum creatinine levels. Moringa leaves (Moringa oleifera L.) and red ginger rhizomes (Zingiber officinale Rosc.) have antioxidant effects. The aim of this study was to determine the inhibitory effect on increasing serum creatinine levels of the concoction Moringa Leaves and Red Ginger Rhizome extract (CML-RGRE). This True Laboratory Experimental Study with Completely Randomized Design was conducted on 30 male Wistar rat dyslipidemia models, induced by high-fat feeding (HFF) and Propylthiouracil (PTU) 0.01% for 21 days. The subjects were divided into 6 groups (n=5), during treatment all subjects were still given HFD and PTU. Groups I, II, III were treated with CML-RGRE dose 135, 202.5, 270 mg/kg BW, IV as dyslipidemia control no added any treatment, comparison control groups V treated with 1.8 mg/kg BW and VI with fenofibrate 5.4 mg/kg BW. Serum creatinine levels (mg/dL) of all subjects were measured after 7 days acclimatization, after creating dyslipidemia models, and after 21 days treatment by using a spectrophotometer with colorimetric kinetic methods. Data of decreased serum creatinine levels were analyzed using ANOVA test, followed by Fischer's LSD, ? = 0.05. All doses of CML-RGRE were very effectively inhibited the increase of serum creatinine levels (p < 0.01). The concoction Moringa leaves, and Red Ginger Rhizome ethanol extract has effectively inhibited the increase of serum creatinine levels of animal dyslipidemia models.

Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef.

Consumers nowadays are becoming more aware of the importance of using only meat products containing safe and natural additives. Hence, using natural food additives for extending the shelf life of meat along with delaying microbial growth has become an urgent issue. Given the increasingly popular view of Moringa oleifera leaves as a traditional remedy and also the scarcity of published data concerning its antimicrobial effect against foodborne pathogens in meat and meat products, we designed the present study to investigate the antimicrobial effect of Moringa oleifera leaves aqueous extract (0.5%, 1%, and 2%) on ground beef during refrigerated storage at 4 °C for 18 days. MLE revealed potent antimicrobial properties against spoilage bacteria, such as aerobic plate count and Enterobacteriaceae count. MLE 2% showed a significant (p < 0.01) reduction in the counts of E. coli O157:H7, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus artificially inoculated to ground beef by 6.54, 5.35, and 5.40 log10 CFU/g, respectively, compared to control, by the 18th day of storage. Moringa leaves extract (MLE) had no adverse effect on the overall acceptability and other sensory attributes; moreover, it induced a slight improvement in the tenderness and juiciness of treated ground beef, compared to the control. Therefore, MLE can be used as a healthy, natural, and safe preservative to increase meat products' safety, quality, and shelf stability during cold storage. A promising approach for using natural food additives rather than chemical preservatives could begin new frontiers in the food industry, as they are more safe and do not constitute health risks to consumers.

Antioxidant Stability of Moringa Leaves Extract Powders Obtained by Cocrystallization, Vacuum Drying, and Plating

Cocrystallization, vacuum drying, and plating are three potential applications to preserve the antioxidant activity of moringa leaves. Moringa leaves extract was incorporated with sucrose at the same concentration (7 : 100, solid : solid) for all applications and stored for 30 days. This study aims to examine the effects of each application on the antioxidant stability of moringa leaves extract powders. Morphological properties by SEM showed that cocrystallized powders exhibited porous, agglomerated crystals, vacuum dried powders exhibited agglomerated crystals, and plated powders exhibited layered crystals. Based on XRD and hygroscopicity results, cocrystallization produced powders with the highest crystallinity, i.e., 69.11%, and the lowest hygroscopicity, i.e., 0.26 × 10−4 ± 0.02 × 10−4 g H2O/g solid/minute due to the slow water intake of the crystalline structure. Powders with the strongest initial antioxidant activity were obtained from cocrystallization, i.e., 3647.96 ± 20.29 ppm and followed by vacuum drying, i.e., 4378.51 ± 26.29 ppm. The least antioxidant activity was obtained from plating, i.e., 4733.46 ± 31.91 ppm. During 30 days of storage, powders obtained by cocrystallization maintained the most stable antioxidant activity (91.81–91.12%). The results indicated that the high temperature used in the process was likely to impact crystalline structure through the pore formation, which entrapped bioactive compounds and resulted in strong antioxidant activity. While, vacuum drying resulted in powders with a lower but increased antioxidant activity (84.06%–86.43%). In contrast to the other two applications, plating resulted in a decreased antioxidant activity (83.77–82.25%). This study suggests that application of cocrystallization produced moringa leaves extract powders with the strongest and most stable antioxidant activity during storage. Preserving the antioxidant stability of plant extract has been one of the major drives in the development of food encapsulation technology. Cocrystallization and vacuum drying are two relatively novel, less common techniques offering a simpler and more cost-effective method, but their effect on the antioxidant stability of moringa leaves extract has not yet been studied. This study discloses the effects of cocrystallization, vacuum drying, and plating (alternative extract incorporation method) on the antioxidant stability of moringa leaves extract powders. The results indicated that the three methods produced powders with high crystallinity and stable antioxidant stability during storage. Among the three methods, cocrystallization was the method that resulted in powders with the strongest and most stable antioxidant activity.

Pre- Sowing Seed Treatment with Panchagavya, Beejamruth and Leaf Extract of Moringa and Neem on Growth, Yield and Yield Attributing Traits of Cowpea (Vigna unguiculata L.) cv-Bali265

The present experiment entitled Pre-Sowing Seed Treatment with Panchagavya,Beejamruth and Leaf Extract of Moringa and Neem on Growth, Yield and Yield Attributing Characters of Cowpea (Vigna unguiculata L.) cv-Bali265. with the aim to identify better pre-sowing treatment for cowpea. During Kharif, 2021, present trail was carried out at the field experimentation center of the Genetics and Plant Breeding, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad. It has 13 priming treatments including Control on Cowpea seeds variety were used to study under field conditions. Field experiment was laid out in Randomized Block Design (RBD) with three replications respectively. T0- Control, T1 Moringa Leaves Extract 2% (8 hrs), T2 Moringa Leaves Extract 4% (8 hrs), T3 Moringa Leaves Extract 6% (8 hrs), T4 Neem Leaves Extract 2% (8 hrs), T5 Neem Leaves Extract 4% (8 hrs), T6 Neem Leaves Extract 6% (8 hrs), T7 Panchagavya 2% (8 hrs), T8 Panchagavya 4% (8 hrs), T9 Panchagavya 6% (8 hrs), T10 Beejamruth 2% (8 hrs), T11 Beejamruth 4% (8 hrs), T12 Beejamruth (8 hrs). To find out Influence of different seed treatment on growth, yield and seed quality parameters of cowpea showed that significant treatment on rate of field emergence, days to 50% flowering, plant height, number of primary branches, days to maturity, number of pods per plant, number of seeds per pod, 100 seed weight, seed yield per plant, seed yield per plot, biological yield, harvest index. Pre-sowing treatment which is concluded from the present study that the seeds of Cowpea (Variety – Bali 265) were treated with T9 Panchagavya 6% (8 hrs) enhanced the above-mentioned characters.

Response of Monarda citriodora L. plant to foliar spraying with extracts of moringa leaves and peels of the pomegranate

Summary Introduction: Monarda citriodora L. plant belongs to the Lamiaceae family. It is a medicinal and aromatic plant, an annual herb, one of important sources of thymol compound. This plant has various purposes such as medicinal properties, food industry, cosmetics, pharmaceuticals industry, a powerful antioxidant and antimicrobial activity. Objective: There is an ongoing need to find safe, natural sources of plant nutrients. Moringa leaves and pomegranate peels extracts are being used as a secure source of natural nutrients, and natural growth regulators on the growth and productivity of plants. Applying these bio stimulants helps avoid the excessive use of industrial materials in agriculture. So, this experiment was carried out to determine the effect of both moringa leaves extract, and pomegranate peels extract as a bio regulator on the productivity of M. citriodora plant. Methods: This experiment was carried out during two seasons (2017/2018 and 2018/2019) at the Experimental Station of Adlya Farm, Sekem Company, ElSharkia Governorate, Egypt, to study the influence of some natural extracts of moringa leaves and pomegranate peels on growth, yield as well as some chemical constituents of the dry herbs, such as total carbohydrate, total flavonoids compounds, total phenolic content, NPK content and essential oil contents and its components and determination of free radical scavenging activity of M. citriodora L. plant. Results: Both extracts of moringa leaves and pomegranate peels positively affected growth, yield, and chemical constituents compared to untreated plants. Moringa leaves extract had more effect compared with pomegranate peels extract. The main essential oil constituents were thymol, p-cymene, and carvacrol compounds. Conclusion: The application of bio-stimulants of moringa leaves extract (MLE) and pomegranate peels extract (PPE) positively affect the growth and production of M. citriodora plant.

Microscopic Identification and Determination of Total Flavonoid Content of Moringa Leaves Extract and Ethyl Acetate Fraction (Moringa oleifera L.)

This research was conducted to identify simplicia microscopically, phytochemical screening and determination of total flavonoid content of extract and ethyl acetate fraction from Moringa (Moringa oleifera L.) leaves using UV-Visible Spectrophotometry method. The experimental design used in this study was to perform microscopic identification of Moringa leaf powder simplicia, make 96% and 70% ethanol extract and ethyl acetate fraction of Moringa leaves from 70% ethanol extract, then carry out phytochemical screening and determination of total flavonoid content with quercetin standards. Phytochemical screening on the ethyl acetate fraction of Moringa leaves included tests for the content of flavonoids, saponins, tannins and alkaloids. The results of microscopic identification of Moringa leaf simplicia showed the presence of calcium oxalate crystals in the form of rosettes, mesophyll and stomata. The result of determination of total flavonoid content in 96% ethanol extract was 16.69 ± 0.74% (w/w), 70% ethanol extract was 10.84 ± 0.49% (w/w), Moringa leaf ethyl acetate fraction 14 .45 ± 0.90% (w/w). The highest total flavonoid content was found in the 96% ethanol extract of Moringa leaves in accordance with the 2017 Indonesian Herbal Pharmacopoeia, that the thick extract of Moringa leaves containing no less than 6.30% (w/w) total flavonoids was calculated as quercetin.

Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

The success of artificial insemination in dogs is significantly influenced by the quality of the semen used. Furthermore, during storage at 5oC, the semen is attacked by free radicals, which adversely affect its quality. Moringa leaves, and carrot extracts are natural ingredients with high antioxidant content, expected to overcome free radical attacks and oxidative stress experienced by sperm during storage. Therefore, this study aims were to examine the effect of adding Moringa leaf extract or carrot juice in coconut water-egg yolk diluent on the sperm quality of Kintamani dogs. The method used a completely random design. Three treatment groups exist for each diluent. The base diluent consists of young coconut water-egg yolk (D1), the base diluent plus moringa leaves extract (D2), and the base diluent plus carrot juice (D3). The life spermatozoa were observed in eight sections, from 0 (control) to 84 hours with 12 hourly observations. Each treatment group was examined for spermatozoa progressive motility, viability, abnormalities, and intact plasma membrane. The results showed that D2 maintained semen quality significantly better (P&lt;0.05) than D1 and D3. However, D3 was better than D1 (P&lt;0.05). It was concluded that the addition of moringa leaves extract in coconut water-egg yolk diluent maintained the best quality of spermatozoa for 60 hours, with motility, viability, abnormality, and intact plasma membrane of 56.67±19.98, 60.83±18.63, 11.83±2, 64, and 55.83±17.45%, respectively.

Effective Treatment of Curry (Murraya koenigii) and Moringa (Moringa oleifera) Leaves Extracts on Melanosis of Pacific White Shrimp (Litopenaeus vannamei) during Chilled Storage

ABSTRACT This study investigated the treatment effect of curry leaves extract (CLE) and moringa leaves extract (MLE) on the melanosis of Pacific white shrimp during 15 days of chilled storage. These extracts have a high number of phytochemicals that have various therapeutic potentials. In this study, shrimp samples were divided into 4 groups; 1% CLE treated, 1% MLE treated, 1:1 (CLE+MLE) treated and control, and stored for 15 days. Biochemical indices and microbiological analysis wereperformedat 3 days interval. In this study, both extracts showed significant results on chemical indices, but highest qualitative results were obtained by the 1:1 (CLE+MLE) treated group. Among the treated groups, 1:1 (CLE+MLE) had a greater effect on microbial spoilage in comparison with the control group. This study revealed that CLE and MLE contain a variety of phytochemicals withdifferent properties that could be utilized as a natural substitute for synthetic antimelanosic agents used to control melanosis in shrimp.

Response of Seeds, Oil Yield and Fatty Acids Percentage of Jojoba Shrub Strain EAI to Mycorrhizal Fungi and Moringa Leaves Extract

Jojoba seeds have a unique storage lipid wax which is suitable as a basic feedstock in the chemical industry. For saving both human health and the environment, there is a continuous need to search for alternative safe natural sources of plant nutrients. Therefore, in this study the effect of mycorrhizal fungi and Moringa oleifera leaves extract on growth, flowering, fruits set, yield and the chemical composition of the jojoba shrub was studied. The application of a combination of treatments of 20 g L−1 mycorrhizal fungi plus 30 g L−1Moringa oleifera leaves extract recorded the maximum mean values of main branch length, length of secondary branches, number of branched nodes, number of secondary branches, flowering percentage, final fruit set percentage, seeds yield per shrub and per hectare, percentage of minerals, proteins as well as oil yield per shrub and per hectare, chlorophyll a and b, N, P, K percentage with a minimum mean value of the number of days until full bloom in both seasons. The maximum percentage of Gadoleic fatty acid was found with the combination treatment of uninoculation plus 10 g L−1Moringa oleifera leaves extract.

Foliar spray of moringa leaves extract enhanced the chemical contents of Lemon Bee balm plant (Monarda ciriodora L.)

Monarda citriodora L. plant is an important aromatic and medicinal plant (Fam. Lamiaceae). It is economical importance due to its rich in Thymol compound in the volatile oil. The dependency on the use of chemical fertilizer as a source of plant nutrients and their high cost is further associated with land soil degradation and environmental pollution. The objective of this study was to investigate the tested of three levels of chemical fertilizers (recommended dose 100% NPK, 300:200:100 kg/fed., 75% and 50% NPK). As well as foliar spray of moringa leaves extract (MLE) as growth promoting at 0.0, 1:40, 1:30 and 1:20 and their interactions between them on essential oil production, total phenolic content, total flavonoids content and antioxidant activity of Monarda citriodora L. plant. The highest level of NPK 100% (RD) improved the most chemical contents followed by 75% and then 50%. The different concentrations of MLE significantly increased the chemical contents as compared to the control treatment. These constituents increased gradually with increasing the concentration of MLE up to (1:20). Under the same level of NPK, MLE application increased the chemical constituents comparing to NPK without MLE spray. Also, it was observed that the use of the natural extract (MLE) combined with low levels of chemical fertilization lead to increase these constituents compared to high levels of the chemical fertilization (NPK) without spraying the MLE extract. So, applying the natural extract of MLE can be partial substitution of the chemical fertilizers and produced a high quality of Monarda citriodora L., plants.

Tannin Contents of Jackfruit Leaves (Artocarpus heterophyllus) Extract and Moringa Leaves (Moringa oleifera) Extract as Functional Additive Feed in Ruminan Livestock

Tannin compounds are polyphenolic compounds that function as antioxidants and inhibit tumor growth in plants, foods and drinks that can dissolve in water and organic solvents. This research’s objective is to identify tannin contents of Jackfruit leaf extract and Moringa leaf extrack as functional additive feed for ruminant. Detecting simple phenolic compounds the extract with 1% FeCl3 solution in water, which gives rise to strong green, red, purple, blue or black colors. The formation of a blackish green or blackish blue color in the extract after adding FeCl3 because tannins will form complex compounds with Fe3 ions. The research’s result shows that phytochemical test of tannins on moringa and jackfruit leaf extracts showed positive results and generated sediment. The conclusion of this study is the presence of tannin compounds in jackfruit and moringa leaves as a functional feed additive to increase the productivity of ruminants.

Influence of Moringa Leaves Extract on Hb Response to Radiation Dose Rates’ Changes: I. Permittivity and Its Thermodynamic Driven State Functions

The harm caused by ionizing radiation is well known and is performed by depositing energy from the radiation source into the irradiated material. Biosystems fight back to maintain their integrity and functions. Doing so requires external help where important supplements are provided to enable the biosystem to challenge destruction done by irradiation in the form of ionization and free radicals. This work investigates the effect of Moringa oleifera leaves extract, MOLE, on two different gamma radiation dose rates’ damages on the biosystem. This is done by estimating physical parameters that belong to dielectric factors, static permittivity, or based upon them as thermodynamic state functions; changes in Helmholtz free energy, internal energy, and entropy of the sample under assessment. Good sensitivity to factors causing changes in the studied system's net dipole moment is noticed, especially within the 1st week. The help of MOLE, a natural supplement, to the biosystem repairing the damage caused by irradiation was tested. Data indicated that MOLE has a powerful influence that could help the biosystem, based on its vast nutrients, trace elements, and antioxidants to fight radiation damage.

Facial Mask Formulation Enriched with Moringa Leaves (Moringa oleifera) Extract and Their Activity as Antioxidants and Antibacterials

Moringa leaves (Moringa oleifera) are useful for skin health as an additional ingredient for cosmetics, such as facial mask. This study aims to determine the optimum formula of Moringa leaves extract in powder mask, evaluate its activity as an antioxidant and antibacterial, while also ascertaining its characteristics, based on the quality requirements of SNI 16-6070-1999 and SNI 16-4399-1996 for cosmetics and skin moisturizers, respectively. Facial mask formula was made through various concentrations of Moringa leaves methanol extract 12.5%, 17.5%, 25%, and 35%, with its optimum formulation determined by panelists’ preference level, based on organoleptic test results. Furthermore, the antioxidant activity was carried out, using the 1,1-diphenyl-2-picrylhydrazil (DPPH) method, while that of the antibacterial towards Staphylococcus aureus bacteria was performed, through the use of the diffusion technique, with the active compound also being analyzed, using GC-MS (Gas Chromatography-Mass Spectrophotometry). The results however, showed that the antioxidant and antibacterial activities in the facial mask formula were lower than when in their extract form. The facial mask formula with the addition of 35% Moringa leaves extract (F4), had the highest inhibitory value of 66,04%, while the antibacterial activity against S. aureus occured at concentrations ≥ 0.125 g/mL. Also, the result of GC-MS analysis of Moringa leaves extract, showed quinic (peak 7, Rt = 21,165 minutes) and linoleic acid as the highest peak (peak 12, Rt = 29,439 minutes). Based on panelists' preference level, the result of Anova and Duncan Test analysis showed that the most optimum facial mask product was with 17.5% Moringa leaves extract (F2), with a score of 3.29. Therefore, all characterization of mask formula with 12.5%, 17.5%, 25%, and 35% additional amount of Moringa leaves extract, have met up with the requirements for SNI 16-6070-1999 and SNI 16-4380-1996 criterias, together with the pH value of 5.45 - 6.02, specific gravity 1 g/mL, emulsion stability 96.57 - 97.05%, and negative microbial contamination.