Spermatozoa Quality of Kintamani Dogs in Coconut Water-Egg Yolk Diluent with Addition of Moringa Leaves and Carrot Extract

Abstract

The success of artificial insemination in dogs is significantly influenced by the quality of the semen used. Furthermore, during storage at 5oC, the semen is attacked by free radicals, which adversely affect its quality. Moringa leaves, and carrot extracts are natural ingredients with high antioxidant content, expected to overcome free radical attacks and oxidative stress experienced by sperm during storage. Therefore, this study aims were to examine the effect of adding Moringa leaf extract or carrot juice in coconut water-egg yolk diluent on the sperm quality of Kintamani dogs. The method used a completely random design. Three treatment groups exist for each diluent. The base diluent consists of young coconut water-egg yolk (D1), the base diluent plus moringa leaves extract (D2), and the base diluent plus carrot juice (D3). The life spermatozoa were observed in eight sections, from 0 (control) to 84 hours with 12 hourly observations. Each treatment group was examined for spermatozoa progressive motility, viability, abnormalities, and intact plasma membrane. The results showed that D2 maintained semen quality significantly better (P<0.05) than D1 and D3. However, D3 was better than D1 (P<0.05). It was concluded that the addition of moringa leaves extract in coconut water-egg yolk diluent maintained the best quality of spermatozoa for 60 hours, with motility, viability, abnormality, and intact plasma membrane of 56.67±19.98, 60.83±18.63, 11.83±2, 64, and 55.83±17.45%, respectively.