Moringa Leaf Extract Research Articles

Folk floral preservatives extend postharvest longevity of Eustoma grandiflorum L.

Lisianthus (Eustoma grandiflorum L.) is one of the most popular cut flowers worldwide due to its multi-flowered stems having single, semi-double and double rose-like flowers in a variety of colors. However, its postharvest life can be short if not handled properly. A study was conducted to evaluate the effectiveness of various homemade folk floral preservative solutions applied as pulses or vase solutions on cut lisianthus stems. Both experiments were laid out individually in completely randomized design, with five replications of two stems each and evaluated in a vase life evaluation room set at 20±2 °C temperature and 50±10% of relative humidity along with 12 h of light from white fluorescent tubes. Results revealed significant differences among different pulsing treatments as 2% sucrose + 150 mg L−1 citric acid had longest vase life (10.3 days), whereas, 2% sucrose + 3 mL L−1 lime juice, 2% sucrose + 5% aloe vera gel, 2% sucrose + 30 mL L−1 moringa leaf extract (MLE) and 2% sucrose + 15 mL L−1 bleach had best flower quality (8.3) and were statistically similar. Moreover, (50:50) lemon/lime soda and water had highest water uptake (98 mL) and ion leakage (326.8%), whereas, 2% sucrose had highest (735 µs cm−1) change in electric conductivity. Among vase treatments when compared to commercial solutions, Floralife Clear Professional Flower Food, Chrysal Clear Universal Flower Food and 2% sucrose + 4 mL L−1 lime juice had longest vase life (16.6, 15.9 and 15.7 days, respectively) and were statistically similar, whereas, highest microbial colonies (6.14 CFU mL−1) were observed in solution having 5% sucrose only and the lowest with the commercial solutions. In summary, lisianthus stems placed in vase treatments had longest vase life, while those pulsed ones had better visible quality and use of sugar with lemon/lime juice as a vase solution produced a similar vase life as commercial preservatives.

Application of natural and synthetic growth promoters improves the productivity and quality of quinoa crop through enhanced photosynthetic and antioxidant activities

Modern agriculture is primarily concerned with enhanced productivity of field crops linked with maximum resources use efficiency to feed the increasing population of the world. Exogenous application of biostimulants is considered a sustainable and ecofriendly approach to improve the growth and productivity of agronomic and horticultural field crops. The present study was carried out to explore the comparative growth enhancing potential of plant biostimulants (moringa leaf extract at 3% and sorghum water extract at 3%) and synthetic growth promoters (ascorbic acid at 500 μM and hydrogen peroxide at 100 μM) on growth, productivity and quality of quinoa crop (cultivar UAF-Q7) because it has gained significant popularity among agricultural scientists and farmers throughout the world due to its high nutritional profile. A field experiment was carried out at the Research Area of Directorate of Farms, University of Agriculture, Faisalabad, Pakistan during quinoa cultivation season of 2016–2017 and repeated during next year (2017–2018). All the foliar treatments enhanced the physiological, biochemical, quality, growth and yield attributes of quinoa as compared to control group. However, maximum improvement was observed in chlorophyll a and b contents, photosynthesis and respiration rates, and water use efficiency by moringa leaf extract (MLE) application. MLE application was also found more responsive regarding the improvement in activities of peroxidase, catalase, superoxide dismutase, phenolics and glycine betaine as compared to other treatments. Mineral elements i.e. K, Ca and N in root as well as in shoot were found the highest in response to MLE application. Similarly, growth (plant fresh and dry biomass, plant length and grain yield) and grain quality parameters (protein, K and Ca) were also significantly enhanced. Application of MLE was found to be a viable approach to improve the growth and quality of produce as compared to synthetic compounds.

Utilization of Nanogold and Nanosilver in Kelor (Moringa Oleifera Lam.) Leaf Extract for Pandemic of Covid-19

This research has the purpose to determine the characteristics of the synthesis of 20 ppm nanogold using TEM, determine the effect of adding nanogold on the antioxidant activity of Moringa leaves, and determine the best concentration that supports increasing antioxidant activity in Moringa leaves. Nanogold and nanosilver were synthesized using bottom-up methods. The concentrations used for Moringa leaf extract and nanogold were 5, 10, 15, 20, 25, and 30 ppm. The concentration of the nanosilver used is 20 ppm. Nanosilver at a concentration of 20 ppm had the best inhibition of antibacterial activity. Testing of antioxidant activity was carried out using the DPPH method which was analyzed with a UV-Vis spectrophotometer. The results of the TEM nanogold test have a dominant cluster size of 22.17 nm. The best test results for the antioxidant activity of Moringa leaf extract at a concentration of 30 ppm was 60.7258 ppm. The addition of nanogold with a greater concentration of 30 ppm Moringa leaf extract resulted in greater antioxidant activity. The results showed that the best concentration of nanogold as a supporter of antioxidant activity in Moringa leaves was 30 ppm with a percent reduction of 79.288 ppm (very strong category).

FORMULASI DAN EVALUASI SEDIAAN MASKER GEL PEEL-OFF EKSTRAK ETANOL DAUN KELOR (Moringa oleifera L.) DENGAN BASIS Na-CMC DAN CARBOPOL

Moringa leaves (Moringa oleifera L.) contain flavonoid compounds, alkaloids, saponins, and tannins which are used as quality parameters of antioxidant preparations. Peel-off facial masks have several benefits, including being able to clean dirt on the face while removing dead skin cells, preventing skin damage caused by exposure to free radicals, such as acne and black spots, removing fine wrinkles due to premature aging, and moisturizing and nourishing the skin. The purpose of this study was to identify the chemical content of Moringa leaf (Moringa oleifera L.) and to formulate and evaluate the peel-off gel mask of the ethanol extract of Moringa leaf (Moringa oleifera L.) which was good and stable using a comparison of Na-CMC and Carbopol bases. . This research method is experimental which was carried out using Na-CMC base: Carbopol from three different formulations with a concentration ratio of F1 (3%: 0.1 %), F2 (4%: 1%), F3 (5%: 1.5 %), each formulation contains 3% Moringa leaf extract (Moringa oleifera L.). Evaluation of the peel-off gel mask preparation was carried out after 2 weeks of storage. The results showed an evaluation test of the peel-off gel mask preparation of Moringa leaf ethanol extract using Na-CMC and Carbopol bases on the homogeneity test of all homogeneous concentrations, the pH test showed that all concentrations of the preparation met the pH requirements, which ranged from 5-6, on the irritation test all the preparation did not cause redness and in the dry time test all preparations met the requirements, namely under 30 minutes. The conclusion of Moringa leaf ethanol extract using Na-CMC and Carbopol bases shows that it can be used as an alternative to cosmetics and can be used as a reference for use as natural cosmetics.

Coconut Milk and Moringa Leaf Extracts Effect on Seedling Production of Ceiba (Ceiba Pentandra L.) in the Nursery

Coconut Milk and Moringa Leaf Extracts Effect on Seedling Production of Ceiba (Ceiba Pentandra L.) in the Nursery

Antioxidant Activity of Moringa oleifera and Olive Olea europaea L. Leaf Powders and Extracts on Quality and Oxidation Stability of Chicken Burgers.

Oxidation is the main cause of quality deterioration in meat-based foods, such as burgers. Antioxidants inhibit the oxidation process; recently, natural antioxidants have gained interest, due to safety concerns. In this study, the effects of leaf powder and crude extracts of both Moringa oleifera and olive in chicken burgers were studied for their antioxidant potential in preventing fat oxidation during storage. Antioxidant activities were evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl). The results showed the highest DPPH radical scavenging with IC50 values of 2.397 ± 0.10 mg/mL in the Moringa leaf. Total phenolic content (TPC) was crude olive extract > crude Moringa extract > olive leaf > Moringa leaf. The total flavonoid content (TFC) was significantly higher in the olive leaf and its crude extract than in the Moringa leaf and its crude extract. The pH, total volatile nitrogen, and sensory properties were affected by the addition of olive and Moringa (leaf and crude extracts) to chicken burgers refrigerated for 20 days. The addition of Moringa and olive leaf powder decreased lipid oxidation and PV after 10 days of storage. In general, Moringa and olive leaf treatments slowed the deterioration of meat, suggesting their use as preservatives to extend the shelf-life of chicken burgers.

Assessment of the Anti-inflammatory Activities of the Moringa Leaf Extract in Periodontitis Cases through IL-6 Cytokine Analysis in Wistar (Rattus novergicus)

BACKGROUND: Periodontitis is a chronic inflammatory disease that causes damage to the supporting structures of the teeth, and if left untreated, it can lead to impaired function, appearance, pain, and tooth loss. Periodontitis is caused by bacteria that adhere to and grow on the tooth surface. The “red complex” bacteria consisted of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. These bacteria will trigger an inflammatory response in the body. Interleukin-6 (IL-6) is an IL that acts both as a pro-inflammatory cytokine, IL-6 is a stimulator for MMP production. To treat the periodontal disease can be through non-surgical therapy as well as surgical therapy, to maximize therapy, it is accompanied by antimicrobial therapy. However, because of the frequent use of antimicrobials, causing antimicrobial resistance in patients, the use of natural ingredients as additional therapy is very necessary for this study using Moringa leaves as a substitute for antimicrobials. AIM: This study aimed to determine the effectiveness of Moringa leaf in influencing the anti-inflammatory cytokine IL-6. The first benefit of this research is to provide scientific information in the field of dentistry regarding the effectiveness of Moringa leaves against red-complex bacteria P. gingivalis as a cause of chronic periodontitis through anti-inflammatory cytokine analysis. METHODS: The type of research that will be used is quasi-experimental with a post-test research design with a control group design. This study used a sample consisted of 30 Wistars (Rattus novergicus) and was divided into two groups based on periodontal tissue sampling as follows treatment group which was treated with extracts Moringa and control group with aquadest irrigation after bacterial induction in the gingival sulcus. Blood samples were taken on days 0, 1, 3, 5, and 7 and centrifuged obtain blood serum and serum cytokine levels (pg/mL) were quantified using a commercial ELISA IL-6 kit. RESULTS: This study obtained the results that there was a decrease in IL-6 in both groups on the 3rd day of observation (D3) where the treatment group given Moringa extract showed a greater decrease in IL-6 levels compared to the control group. There is a significant value in the comparison of IL-6 levels between the two groups with p-value: 0.000 (p < 0.05). CONCLUSION: Moringa oleifera leaf extract showed the anti-inflammatory effect on inflammation induced by the bacterium P. gingivalis. M. oleifera extract can reduce the production of the pro-inflammatory cytokine IL-6 induced by P. gingivalis bacteria in periodontitis.

Alkaloids, Flavonoids, Tannins and Saponins Contents in Moringa Oleifera Leaves

Various studies have shown that Moringa oleifera leaves have benefits in preventing disease. It also has the potential to solve environmental problems as mosquito larvasides. This is presumably because Moringa leaves contain potentially beneficial compounds. Objective: The aim of this study was to analyze the content of bioactive compounds in Moringa oleifera leaf extract qualitatively and quantitatively. This study was an descriptive study. The study was conducted in Kupang East Nusa Tenggara at September 2019. Moringa leaf extraction was carried out using maceration method with 96% ethanol solvent. The identification of compounds was qualitatively carried out using Thin Layer Chromatography (TLC) methods, while quantitative using spectrophotometric methods. Data was analyzed descriptively to describe the content of flavonoid, tannin, alkaloid, saponin. Study results showed that Moringa leaf extract had flavonoid, alkaloid, tannin and saponin components which were measured qualitatively. Quantitative measurement results showed that Moringa leaves have compounds Total alkaloid Equivalent Quinine 0,3% b/b, Total Flavonoid Equivalent Quercetin17,40 % b/b, Tannin Total Equivalent Tannic Acid 14,68 % b/b, Saponin 7,41% b/b. It is concluded that Moringa oleifera leaf extract has compounds that are beneficial for many things, both in disease prevention, larvasides and other benefits.

The Impact of Bio-Stimulants on Cd-Stressed Wheat (Triticum aestivum L.): Insights Into Growth, Chlorophyll Fluorescence, Cd Accumulation, and Osmolyte Regulation.

It has been established that wheat (Triticum aestivum L.) has a higher Cd absorption capacity than other cereal crops causing an excess daily Cd intake and a huge threat for public health. Therefore, the reduction of Cd accumulation in wheat from the soil is a crucial food-security issue. A pot trial was performed on Cd-stressed wheat seedlings to evaluate the morphological and physio-biochemical responses via foliage spray of two different bio-stimulants, i.e., ascorbic acid (AsA) and moringa leaf extract (MLE). Two wheat cultivars (Fsd-08 and Glxy-13) were exposed to cadmium (CdCl2.5H2O) stress (0, 500, and 1,000 μM), along with foliar spray of AsA (0 and 50 mM) and MLE (0 and 3%). The most observable growth reduction was documented in plants that are exposed to a higher Cd concentration (1,000 μM), followed by the lower Cd level (500 μM). The wheat growth attributes, such as number of leaves per plant, number of tillers per plant, biomass yield, shoot/root length, and leaf area, were greatly depressed under the Cd stress, irrespective of the cultivar. Under the increasing Cd stress, a significant diminution was observed in maximum photochemical efficiency (Fv/Fm), photochemical quenching (qP), and electron transport rate (ETR) accompanied with reduced gas exchange attributes. However, Cd-induced phytotoxicity enhanced the non-photochemical quenching (NPQ) and internal carbon dioxide concentration (Ci), which was confirmed by their significant positive correlation with Cd contents in shoot and root tissues of both cultivars. The contents of proline, AsA, glycine betaine (GB), tocopherol, total free amino acid (TFAA), and total soluble sugar (TSS) were greatly decreased with Cd stress (1,000 μM), while MLE and AsA significantly enhanced the osmolytes accumulation under both Cd levels (especially 500 μM level). The Cd accumulation was predominantly found in the root as compared to shoots in both cultivars, which has declined after the application of MLE and AsA. Conclusively, MLE was found to be more effective to mitigate Cd-induced phytotoxicity up to 500 μM Cd concentration, compared with the AsA amendment.

REKAYASA TABLET EFFERVESCENT DARI TOMAT DAN DAUN KELOR DENGAN PERBEDAAN RASIO ASAM DAN BASA

This study aimed to produce the best formulation in the manufacture of effervescent derived from tomatoes and Moringa leaves with different acid-base ratio treatments. Tomato and Moringa leaves were converted into powdered extracts to be formulated with citric acid, sodium bicarbonate, PVP, mannitol, and aspartame. The ratios of citric acid and sodium bicarbonate as treatments were 3:5, 4:5, and 5:5. The results showed that the formulation with an acid:base ratio (5:5) had the potential to be developed and refined before being commercialized. This formulation was chosen because it has the sensory profile with a sweet, slightly sour and fresh taste profile, the lowest Moringa leaf aroma, the color of the clear solution was slightly green and slightly orange. This formulation was made from a composition of 12.5% tomato extract powder, 7.5% Moringa leaf extract powder, 34% citric acid, 34% sodium bicarbonate, 7.5% mannitol, 3% PVP, and 1.5% aspartame. Tablet was carried out in a room with a temperature of 18℃ with a RH of 45-58%.

Foliar application of potassium and moringa leaf extract improves growth, physiology and productivity of kabuli chickpea grown under varying sowing regimes.

Chickpea (Cicer arietinum L.) is of prime importance because of vital source of protein as major food legume. Globally, it is cultivated on large area to meet dietary requirements of humans. Climatic extremes (erratic rainfall, extreme high and low temperature) are key restrains for its production. Optimum sowing time is considered as an important factor to address climatic variations and to attain maximum yield. Foliar application of potassium (K) has also been reported to increase resistance against abiotic stresses. Similarly, exogenous application of plant based growth substances (bio-stimulants) like moringa leaf extract (MLE) are extensively used to enhance productivity of field crops. Therefore, current study was planned to evaluate the impact of foliar applied K and MLE on growth, physiology and productivity of kabuli chickpea grown under varying sowing dates. There were two sowing dates (normal sown; November 15 and late sown; December 15, 2020). Experiment was comprised of treatments i.e. control, water spray, foliar application of K at 1%, foliar application of MLE at 3% and combined application of K and MLE. Foliar applied K and MLE significantly improved physiological, biochemical and yield attributes of kabuli chickpea cultivated under normal and late sown conditions. Increase in growth and yield attributes like plant height, number of nodules per plant, nodules dry weight, branches and pods per plant, 100- grain weight, biological and grain yield were recorded in case of combined foliar application of K and MLE in normal and late sown chickpea. Maximum improvement in gas exchange attributes (stomatal conductance and transpiration rate), chlorophyll contents, antioxidants (catalase, superoxide dismutase and ascorbate peroxidase) and osmolytes (proline) were recorded with combined application of K and MLE in both sowing dates. Thus, combined applied K and MLE can be used to enhance productivity of kabuli chickpea.

Alleviation of temperature stress in maize by integration of foliar applied growth promoting substances and sowing dates.

Temperature is a key factor influencing plant growth and productivity, but its sudden rise can cause severe consequences on crop performances. Early sowing and application of growth promoting agents as a foliar spray can be a sustainable approach to cope with high temperature stress at grain filling stage of cereal crops. Therefore, a test was designed to explore the potential of different growth helping agents including sorghum water extract (SWE, 10 ml L-1), moringa leaf extract (MLE, 3%), hydrogen peroxide (H2O2, 2 μM), salicylic acid (SA, 50 mg L-1) and ascorbic acid (ASA, 50 mg L-1) as foliar agents at different sowing dates (early and optimum) to cope with temperature stress in maize. The results stated that foliar application of growth promoting substances successfully persuaded high temperature tolerance at reproductive phase of maize in early and optimum sowings when compared to control. However, SWE + ASA, MLE + H2O2 and SWE + ASA + SA + H2O2 were the best combinations for improving growth, development, and physiological variables under both sowing dates even under suboptimal temperature. All foliar applications significantly increased maize grain and biological yields while maximum was observed in SWE + ASA followed by SWE + ASA + SA + H2O2 or MLE + H2O2 that were statistically at par with ASA + SA + H2O2 but plants without spray or distilled water application did not improve grain and biological yields. Overall, the foliar applications of growth promoting substances enable the plant to enhance its growth, development, morphology, yield and biochemical variables.

Application of Moringa Leaf Extract as a Seed Priming Agent Enhances Growth and Physiological Attributes of Rice Seedlings Cultivated under Water Deficit Regime.

Population growth, food shortages, climate change and water scarcity are some of the frightening challenges being confronted in today’s world. Water deficit or drought stress has been considered a severe limitation for the productivity of rice, a widely popular nutritive cereal crop and the staple food of a large portion of the population. A key stage in crop growth is seed emergence, which is mostly constrained by abiotic elements such as high temperatures, soil crusting and low water potential, which are responsible for poor stand establishment. Seed priming is a pre-sowing treatment of seeds that primes them to a physiological state that allows them to emerge more proficiently. The purpose of this study was to investigate the potential of leaf extracts from local and exotic moringa landraces as seed priming agents in rice cultivated under water deficit (75% field capacity) and control conditions (100% field capacity). Rice seeds were placed in an aerated solution of moringa leaf extract (MLE) at 3% from three obtained landraces (Faisalabad, Multan and an exotic landrace of India). The results obtained from the experimentation show that the water deficit regime adversely affected the studied indicators including emergence and growth attributes as well as physiological parameters. Among the priming agents, MLE from the Faisalabad landrace significantly improved the speed and spread of emergence of rice seedlings (time to start emergence at 23%, emergence index at 75%, mean emergence time at 3.58% and final emergence percentage at 46%). All the priming agents enhanced the growth, photosynthetic pigments, gas exchange parameters and antioxidant activities, particularly under the water deficit regime, but the maximum improvement was recorded by the MLE from the Faisalabad landrace. Therefore, the MLE of the Faisalabad landrace can be productively used to boost the seedling establishment and growth of rice grown under normal and water deficit conditions.

Micronutrient and redox homeostasis contribute to Moringa oleifera-regulated drought tolerance in wheat

Global food security is being severely affected by the rapid increase in population and drastic climate change. Drought stress is the most important limiting factor for the sustainable production of several important crops, including wheat. The gradual temperature rise and reduced precipitations are likely to cause the frequent onset of droughts around the world. Therefore, alleviation of drought stress in crop plants has become an essential requirement to meet the increasing food demand. The present study explored the role of foliar application of Moringa leaf extract (MLE) in conferring drought tolerance in wheat during the anthesis stage. A wheat genotype of Indo-Gangetic Plains (HI1544) was exposed to drought stress during the anthesis of the spikes and simultaneously foliar sprayed with MLE for 10 days. The results showed the MLE treatment to improve the concentrations of macro- (K, Ca) and micronutrients (B, Cu, Fe, Mn, Zn, Si) in flag-leaves of wheat under non-stressed conditions. Application of MLE also maintained the flag-leaf nutritional contents under drought stress. The micro-nutrients, including Cu, Fe, Mn, and Zn being the co-factors of the enzymes also stimulated the antioxidant enzyme activities; eventually leading to a significant reduction in the reactive oxygen species and malondialdehyde accumulations under drought stress. Furthermore, micronutrients played a crucial role in osmotic adjustment and sustainable plant growth under drought stress. Overall, the study provided insights into the functional role of micronutrients in improving drought tolerance and also indicated the potential to commercialize MLE as an effective bio-stimulant for sustainable agriculture in drought-prone regions.

How Does the Addition of Biostimulants Affect the Growth, Yield, and Quality Parameters of the Snap Bean (Phaseolus vulgaris L.)? How Is This Reflected in Its Nutritional Value?

Recently, the use of biostimulants as natural and eco-friendly fertilizers has received increasing attention because of their efficiency in terms of improving crops’ qualitative and quantitative parameters, i.e., growth, yield, and chemical composition. We studied the effect of four biostimulants—humic acid (20 g/L), vermicompost tea (15 mL/L), moringa leaf extract (1:30 v/v), and yeast extract (5 g/L), with tap water as a control treatment—on the qualitative and quantitative characteristics of snap beans. The experiment was designed using a complete randomized block with triplicates. The results showed a significant improvement in treated plant performance (growth and yield), chlorophyll, and chemical composition compared to untreated plants. Using moringa leaf extract increased the plant height, number of leaves and branches/plant, and fresh and dry weight. Additionally, the diameter of the treated plant stems and the quality of the crop and pods were also significantly higher than those of plants treated with vermicompost or humic acid extract. It is also noted that the profile of amino acids was improved using all tested biostimulants. This leads to the conclusion that the addition of moringa leaf extract and vermicompost tea not only positively affects the qualitative and quantitative properties of snap bean but is also reflected in its nutritional value as a plant-based food.

The Effects of a Gum Arabic-Based Edible Coating on Guava Fruit Characteristics during Storage

Guava is a nutritious fruit that has perishable behavior during storage. We aimed to determine the influences of some edible coatings (namely, cactus pear stem (10%), moringa (10%), and henna leaf (3%) extracts incorporated with gum Arabic (10%)), on the guava fruits’ properties when stored under ambient and refrigeration temperatures for 7, 14, and 21 days. The results revealed that the coating with gum Arabic (10%) only, or combined with the natural plant extracts, exhibited a significant reduction in weight loss, decay, and rot ratio. Meanwhile, there were notable increases in marketability. Moreover, among all tested treatments, the application of gum Arabic (10%) + moringa extract (10%) was the superior treatment for most studied parameters, and exhibited for the highest values for maintaining firmness, total soluble solids, total sugars, and total antioxidant activity. Overall, it was suggested that coating guava with 10% gum Arabic combined with other plant extracts could maintain the postharvest storage quality of the cold-storage guava.

PHYSICOCHEMICAL PROPERTIES AND NUTRITION OF MORINGA OLEIFERA LAM. LEAF EXTRACT: A PRELIMINARY STUDY ON PREPARATION PHYTOSOMES AS HERBAL SUPPLEMENT FOR CHILDREN

Objective: The study aimed to evaluate the physicochemical properties and nutrition of Moringa leaf extract. In addition, the preliminary study for the preparation of Moringa leaf extract-loaded phytosomes for a supplement. Methods: Extraction of Moringa leaf made using microwave-assisted extraction, followed by evaluation of proximate analysis (water, total ash, acid-insoluble of ash contents, and residual n-hexane), phytochemical screening, and nutrition such as crude protein, amino acids, and minerals (iron, zinc, and calcium). The phytosomes were prepared by the anti-solvent precipitation method and assessed for the morphology, particle size, zeta potential, polydispersity index (PDI), entrapment efficiency (EE), and Fourier-transform infrared spectra. Results: The nutrition contents of crude protein, iron, zinc, and calcium were 19.61±0.07%, 3.47±0.00 mg/100g, 5.46±0.05 mg/100g, and 747.40±4.89 mg/100g, respectively. The amino acids with the highest concentrations were glutamic acid, phenylalanine, aspartic acid, alanine, and arginine in the extract. The best preparation using sonication 10 min by morphology was a spherical included particle size, PDI, zeta potential, and EE of arginine was 87.16±1.73 nm, 0.22±0.04, −23.07±0.76 mV, and 108.94±0.52%, respectively. Conclusion: These preliminary results provide evidence of the nutritional benefit of Moringa leaf extract-loaded phytosomes as a promising supplement to prevent stunting in children.

EFFICACY OF SOME BOTANICAL EXTRACTS AGAINST INFESTATION OF INSECT PESTS OF OKRA (ABELMOSCHUS ESCULENTUS L.)

The experiment was conducted to determine the efficacy of some botanical extracts using Neem leaf extracts, Azadiracta indica, Moringa leaf extract Moringa oleifera, Jatropha leaf extract Jatropha gossypifolia and systemic insecticides (Cypermethrin) as treatments. The treatments were arranged in Completely Randomized Design (CRD), replicated three times. Data were collected on parameters such as, number of leaf, plant height, days to 50% flowering, fresh fruit weight and yield weight were subjected to Analysis of Variance (ANOVA). Results showed that Moringa leaf extracts had the least insect pests’ damage (0.33), tallest plant height (44.00 cm), highest leaf number (8.00), heaviest fruits weight (46.94 g) and highest fruit yield among the botanicals used. Hence, the leaf of Moringa (Moringa oleifera) can be recommended to be used for managing insect pests of okra.

Effectiveness of Methanol Extract of Moringa oleifera Lam. Leaf as Antibacterial Drug to Bacterial Triggers of Urinary Tract Infections In vitro

Background: Urinary tract infections (UTIs) are a severe public health problem and are caused by a range of pathogens, but most commonly by Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterococcus faecalis and Staphylococcus saprophyticus. High recurrence rates and increasing antimicrobial resistance among uropathogens threaten to greatly increase the economic burden of these infections. This study aimed to measure the lowest concentration of Moringa oleifera Lam. Leaf that did not show any growth of the tested microorganisms.
 Methods: The antibacterial activity test was adapted from the CLSI broth microdilution assay, with minor modifications, were used for the evaluation of the antibacterial activity of M. oleifera extracts against tested bacteria. Three concentrations higher than the MIC standard were cultured on the MHA. The lowest concentration on agar which was not found any growth of the bacterial colonies was determined as the MBC.
 Results: The antibacterial activity test results showed that Moringa leaf extract could inhibit bacterial growth. Inhibition of bacterial growth based on extract concentration. For E. coli bacteria, the results showed the highest absorbance value at 100mg/ml concentration (2.86±0.02); even when diluted to 3,13 mg/ml (1.08±0.01), the moringa leaf extracts could inhibit the E. coli growth, although the value did not differ greatly from that of the negative control (0.13±0.06). The same as E. coli, the antibacterial activity test results showed that moringa leaf extract could inhibit others bacteria growth of UTIs.
 Conclusions: Methanol extract of Moringa oleifera leaf can inhibit and bactericide the growth of urinary tract infections bacteria.

Formulation, Evaluation of Physical Properties, and In Vitro Antioxidant Activity Test of Moringa Leaf (Moringa oleifera L.) Ethanolic Extract Capsules

BACKGROUND: Supplements that contain antioxidants may enhance prevention and treatment effects of a wide range of diseases including COVID-19. Quercetin, a flavonoid compound, is a natural antioxidant that can neutralize free radicals. The current study was conducted to formulate Moringa leaf (Moringa oleifera L) ethanol extract capsules and to determine the quercetin antioxidant activity levels of Moringa ethanol extract capsule formulations. 
 MATERIALS AND METHODS: We tested the total flavonoid levels in solutions with concentrations of 20, 50, 60, 70 and 100 ppm using Thin Layer Chromatography Densitometric method. Evaluation of physical properties of 96% Moringa leaf ethanol extract capsules included moisture content test, granule angle of repose test, granule flow property test, capsule weight uniformity test and capsule disintegration time test. Antioxidant activity test using the DPPH method with two samples, namely 96% Moringa leaf ethanol extract capsules with formulas I, II, III, quercetin as a comparison. 
 RESULT: The results of the evaluation of 96% Moringa leaf ethanol extract capsules showed that formula II (PVP 50 mg) had good physical properties. Testing the antioxidant activity of capsules of ethanol extract of Moringa leaves formulas I, II, III, quercetin obtained IC50 values of 44.0 ppm, 40.2 ppm, 46.4 ppm, and 4.80 ppm, respectively. 
 CONCLUSION: The evaluation of the ethanol extract capsules of Moringa leaf formula II, met the parameters of a good capsule evaluation test requirement and had very strong antioxidant activity seen from the acquisition of the IC50 value. The antioxidant properties of Moringa leaf extract capsules may be able to improve the immune system and clinical trials need to be carried out on patients to become candidates for prevention and therapeutic supplement fora range of diseases including COVID-19.